FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 36h_36.34100000005de8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 36h_36.34100000005de8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences327706
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGTC22370.6826240593702892No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGAA9870.3011845983900203No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCAT7490.22855852501937712No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGCC4380.13365638712748623No Hit
GAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGAAACATCCACTTGA3660.11168547417502273No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTAG3610.11015971633110165No Hit
GTCCTTCAGTGTGCATTTCTCATTTTTCACGTTTTTTAGTGATTTCGTCAT3390.10344638181784892No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTCT356.2349172E-638.57116314
TGCCGTC2550.037.0585745
ACTGCGA2700.036.66641627
CTGCGAC2700.036.66641628
TGCGACC2700.036.66641629
GCGACCT2750.035.99975630
ACGAGAC2750.035.99975622
TATGCCG2650.035.66013743
CGACCTA2800.035.356931
CCCACGA2800.035.356919
ATGCCGT2700.034.99976344
GACCTAT2900.034.91355532
CCACGAG2850.034.73660720
ATCTCGT2950.034.321837
GCCCACG2900.034.137718
TCTCGTA2900.034.137738
TCGTATG2900.034.137740
CGAGACT2900.034.137723
GACTGCG2900.034.137726
CTCGTAT2950.033.55909339
Produced by FastQC (version 0.11.2)