FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 36h_35.34100000005ddb.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 36h_35.34100000005ddb.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences328500
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGTC17270.5257229832572298No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGAA6290.19147640791476406No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCAT5950.18112633181126334No Hit
GAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGAAACATCCACTTGA4570.13911719939117198No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGCC3360.10228310502283106No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCTA2450.038.5703831
CACGAGA2600.037.2105321
GCGACCT2550.037.06345730
TGCGACC2550.037.06345729
TATGCCG2700.036.66567643
ACGAGAC2650.036.50844622
TGCCGTC2650.036.50844645
TATCCCC250.00210520335.99902334
ATGCCGT2700.035.83236344
CCACGAG2700.035.83236320
ACTGCGA2650.035.6648427
CTGCGAC2650.035.6648428
TCTCGTA2750.035.18086638
CGAGCCC2750.035.18086615
TCTCCGA2750.035.18086611
GACTGCG2700.035.0043826
CGAGACT2700.034.99905423
CGTATGC2800.034.55263541
GACCTAT2750.034.36270532
GCCCACG2850.033.9464518
Produced by FastQC (version 0.11.2)