##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename H23NVBCXX l02n01 36h_34.34100000005dce.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 70426 Sequences flagged as poor quality 0 Sequence length 51 %GC 36 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.339093516598986 38.0 38.0 38.0 32.0 38.0 2 36.33679322977309 38.0 38.0 38.0 32.0 38.0 3 36.27755374435578 38.0 38.0 38.0 32.0 38.0 4 36.36727912986681 38.0 38.0 38.0 32.0 38.0 5 36.39415840740636 38.0 38.0 38.0 32.0 38.0 6 38.31418794195326 40.0 38.0 40.0 38.0 40.0 7 38.18774316303638 40.0 38.0 40.0 38.0 40.0 8 38.17815863459518 40.0 38.0 40.0 38.0 40.0 9 38.16797773549541 40.0 38.0 40.0 38.0 40.0 10 38.09478033680743 40.0 38.0 40.0 38.0 40.0 11 38.08796467213813 40.0 38.0 40.0 38.0 40.0 12 37.97992218782836 40.0 38.0 40.0 32.0 40.0 13 37.98503393633033 40.0 38.0 40.0 32.0 40.0 14 37.87757362337773 40.0 38.0 40.0 32.0 40.0 15 37.92509868514469 40.0 38.0 40.0 32.0 40.0 16 37.92106608354869 40.0 38.0 40.0 32.0 40.0 17 37.977110726152276 40.0 38.0 40.0 32.0 40.0 18 37.98595689092097 40.0 38.0 40.0 32.0 40.0 19 38.0252321585778 40.0 38.0 40.0 32.0 40.0 20 38.018132507880615 40.0 38.0 40.0 32.0 40.0 21 38.02428080538437 40.0 38.0 40.0 32.0 40.0 22 38.03761394939369 40.0 38.0 40.0 32.0 40.0 23 38.00633288842189 40.0 38.0 40.0 32.0 40.0 24 38.013262147502346 40.0 38.0 40.0 32.0 40.0 25 37.997486723653196 40.0 38.0 40.0 32.0 40.0 26 37.931715559594466 40.0 38.0 40.0 32.0 40.0 27 37.8713401300656 40.0 38.0 40.0 32.0 40.0 28 37.88198960611138 40.0 38.0 40.0 32.0 40.0 29 37.81404594893931 40.0 38.0 40.0 32.0 40.0 30 37.78062079345696 40.0 38.0 40.0 32.0 40.0 31 37.79098628347485 40.0 38.0 40.0 32.0 40.0 32 37.749240337375404 40.0 38.0 40.0 32.0 40.0 33 37.73269815125096 40.0 38.0 40.0 32.0 40.0 34 37.70303581063812 40.0 38.0 40.0 32.0 40.0 35 37.68774316303638 40.0 38.0 40.0 32.0 40.0 36 37.61972850935734 40.0 38.0 40.0 32.0 40.0 37 37.58924260926362 40.0 38.0 40.0 32.0 40.0 38 37.565472978729446 40.0 38.0 40.0 32.0 40.0 39 37.507042853491605 40.0 38.0 40.0 32.0 40.0 40 37.45052963394201 40.0 38.0 40.0 32.0 40.0 41 37.42568085650186 40.0 38.0 40.0 32.0 40.0 42 37.38988441768665 40.0 38.0 40.0 32.0 40.0 43 37.305781955527785 40.0 38.0 40.0 32.0 40.0 44 37.225513304745405 40.0 38.0 40.0 32.0 40.0 45 37.165350864737455 40.0 38.0 40.0 32.0 40.0 46 37.05764916366115 40.0 38.0 40.0 32.0 40.0 47 36.98695084201857 40.0 38.0 40.0 32.0 40.0 48 36.909010876664865 40.0 38.0 40.0 32.0 40.0 49 36.82588816630222 40.0 38.0 40.0 32.0 40.0 50 36.77979723397609 40.0 38.0 40.0 32.0 40.0 51 36.41224831738278 38.0 38.0 40.0 27.0 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2201 1 -0.257690591761488 2201 2 0.15453485260176336 2201 3 0.31745382319707005 2201 4 0.28657137892174944 2201 5 0.4742600096897007 2201 6 0.4454002556715153 2201 7 0.08658414092270306 2201 8 0.10714133775239532 2201 9 -0.41088312538783356 2201 10 -0.6432525254664583 2201 11 -0.20392855849375735 2201 12 -0.041312243127549664 2201 13 -0.12596269501114676 2201 14 -0.6227425740978063 2201 15 0.465495421796426 2201 16 0.020354577885242975 2201 17 0.5317966800285916 2201 18 0.011026419918920283 2201 19 0.4188751540811282 2201 20 0.32670932563481614 2201 21 -0.17770378323581326 2201 22 -0.4372095360384094 2201 23 -0.09861717453635777 2201 24 0.1549001035433264 2201 25 -0.11934558922948924 2201 26 0.15850313017126183 2201 27 -0.45657833825428895 2201 28 -0.5286790916521795 2201 29 0.330183673168051 2201 30 -1.0727195297446954 2201 31 -0.4369939454980525 2201 32 -0.6031140202713914 2201 33 -0.8823751279323488 2201 34 -0.47486762032563234 2201 35 -0.49683934108866623 2201 36 0.5457075451534408 2201 37 -0.36234586748170017 2201 38 0.5390884338234798 2201 39 0.7594160873096669 2201 40 0.10312227173106692 2201 41 0.38810377926616724 2201 42 -0.43771411905007795 2201 43 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42 -0.09858368426746722 1212 43 0.3186920920281082 1212 44 -0.38943282009299907 1212 45 0.35481244523663946 1212 46 0.3745096097504259 1212 47 0.06123795481294536 1212 48 0.33588790064600715 1212 49 -0.32147660118159394 1212 50 0.863751973518049 1212 51 0.3275619073836751 1213 1 -0.31609723887780916 1213 2 -0.2320731630385069 1213 3 -0.34530277504340035 1213 4 0.14189884715244716 1213 5 -0.12960118288116718 1213 6 0.3852829536187272 1213 7 0.006427738185657006 1213 8 -0.5404637453365524 1213 9 -0.4243239856028893 1213 10 0.1771287062050959 1213 11 -0.02015534246248052 1213 12 -0.3343229958157252 1213 13 -0.37254138513822 1213 14 0.02901695669398663 1213 15 -0.5638300914000567 1213 16 0.1682040402508349 1213 17 -0.2489951088277138 1213 18 -0.8115542252423751 1213 19 -0.365582323924734 1213 20 0.004617439026802117 1213 21 -0.6048787587978879 1213 22 -0.007835146986600705 1213 23 -0.8403082791306886 1213 24 -0.6693911965544288 1213 25 -0.4292185120056402 1213 26 -0.4082124123507356 1213 27 0.09425255128628152 1213 28 -0.07120108578708084 1213 29 0.39787673279659685 1213 30 0.09492465402851735 1213 31 -0.27106041666129954 1213 32 -0.48336817471909654 1213 33 0.3104401213345156 1213 34 0.06130051261669678 1213 35 0.5041381760178822 1213 36 0.15470070253369528 1213 37 0.38032275421917916 1213 38 -0.20040325728111696 1213 39 0.04240728965570639 1213 40 0.7433959765209011 1213 41 -0.14244363031350105 1213 42 0.4183464870105311 1213 43 0.1471505900518224 1213 44 0.758656771474854 1213 45 0.5331391909546923 1213 46 0.47312621054093995 1213 47 0.8345581129157083 1213 48 -0.26279457629733827 1213 49 0.38267359644686394 1213 50 0.45571508287246587 1213 51 -0.23113374479023463 >>END_MODULE >>Per sequence quality scores pass #Quality Count 15 2.0 16 3.0 17 5.0 18 6.0 19 11.0 20 9.0 21 26.0 22 33.0 23 63.0 24 131.0 25 204.0 26 337.0 27 500.0 28 627.0 29 881.0 30 1096.0 31 1387.0 32 1679.0 33 2098.0 34 2531.0 35 3138.0 36 4128.0 37 5977.0 38 10835.0 39 34719.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 37.42790578742506 24.830241213739807 14.691593033497172 23.050259965337954 2 12.222932197373092 25.57046503372382 40.79801206957757 21.408590699325526 3 18.280180615113736 29.802913696646126 31.494050492715758 20.42285519552438 4 11.68176525714935 23.1917189674268 36.57314060148241 28.553375173941443 5 11.718683440774715 39.06653792633402 35.317922358219974 13.896856274671284 6 27.843410104222873 42.409053474569056 15.80098259165649 13.946553829551586 7 21.07886291994434 37.97319171896743 21.114361173430268 19.83358418765797 8 21.225115724306363 40.72217646891773 20.702581432993497 17.35012637378241 9 21.7732087581291 21.696532530599494 22.535711243006844 33.994547468264564 10 14.088546843495301 33.980348166870186 31.361996989748103 20.569107999886405 11 27.52005679801207 27.47887823926163 25.79481718139865 19.206247781327654 12 20.664243319228696 29.579984664754495 30.585295203475987 19.170476812540823 13 24.63720784937381 26.363842898929374 29.18524408599097 19.813705165705848 14 18.09558969698691 27.07522789878738 29.682219634793967 25.146962769431745 15 19.969897481043933 34.135120552068834 26.514355493709708 19.38062647317752 16 18.689385871494498 33.95669151579695 28.475683351082715 18.878239261625843 17 16.48680884900463 35.17024962371851 30.441882259392838 17.90105926788402 18 18.034788782392617 30.217962371317004 35.31274405395811 16.43450479233227 19 17.467980575355693 31.11492914548604 32.77198761820919 18.64510266094908 20 17.107318319938656 30.82952318745918 33.29878169994036 18.764376792661803 21 15.803822451935364 31.728338965722884 33.048873995399425 19.418964586942323 22 16.20424275125664 34.79680799704655 33.03041490358674 15.968534348110072 23 15.36364410870985 32.06202254849062 33.86391389543634 18.71041944736319 24 18.474711044216622 31.969727089427202 34.56819924459717 14.98736262175901 25 16.469769687331386 33.8468747337631 33.736120182887 15.947235396018517 26 17.383031593894213 32.44728434504792 34.6283280085197 15.54135605253816 27 16.022491693408682 34.17771845625195 33.82557578167154 15.974214068667822 28 15.298327322295743 32.02936415528356 33.8355152926476 18.836793229773093 29 16.704058160338512 31.61758441484679 35.36052026240309 16.317837162411607 30 17.823469271839144 31.822958082471885 33.541122344655236 16.81245030103374 31 17.165535455655583 31.66728196972709 32.864283077272596 18.30289949734473 32 17.82580297049385 32.75920824695425 33.32576037258967 16.08922840996223 33 15.407661943032402 34.61221707891971 34.21321670973788 15.76690426831 34 15.833640984863544 32.11597989378922 34.004486979240625 18.04589214210661 35 15.772583988867748 32.63425439468378 33.58560758810666 18.007554028341804 36 15.221651094766136 32.85718342657542 35.94979127026951 15.971374208388948 37 15.60787209269304 34.37366881549428 33.17666770794877 16.841791383863914 38 16.01965183312981 32.75778831681481 34.91466219861983 16.30789765143555 39 16.476869338028568 31.716979524607392 33.17666770794877 18.629483429415274 40 16.817652571493483 31.239882997756514 35.768040212421546 16.174424218328458 41 16.388833669383466 31.271121460824126 33.8738534064124 18.466191463380003 42 18.186465225910887 31.82915400562292 33.658024025217955 16.326356743248233 43 15.488597960980318 32.11597989378922 35.87169511260046 16.523727032629996 44 15.362224178570413 34.15215971374209 33.09289182972198 17.392724277965524 45 15.485758100701446 32.08332150058217 35.124811859256525 17.306108539459856 46 18.09416976684747 31.417374265186155 32.443983755999206 18.04447221196717 47 16.41581234203277 31.47843126118195 32.35026836679636 19.755488029988925 48 15.587993070740918 31.759577428790504 32.221054724107574 20.431374776361004 49 18.125408229915088 31.38897566239741 32.632834464544345 17.852781643143157 50 15.745605316218441 32.6924715304007 33.71198137051657 17.849941782864285 51 15.20525964528634 32.17272765999745 32.54050523266546 20.08150746205075 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2251.0 1 1254.5 2 258.0 3 329.5 4 401.0 5 396.5 6 392.0 7 460.0 8 528.0 9 501.0 10 474.0 11 772.5 12 1071.0 13 1517.0 14 1963.0 15 2136.0 16 2309.0 17 1802.0 18 1295.0 19 1030.5 20 766.0 21 957.0 22 1148.0 23 928.0 24 708.0 25 754.0 26 1197.0 27 1594.0 28 1902.0 29 2210.0 30 3019.0 31 3828.0 32 4588.0 33 5348.0 34 5591.0 35 5834.0 36 5604.5 37 5375.0 38 4919.5 39 4464.0 40 3972.5 41 3481.0 42 3225.5 43 2970.0 44 2887.5 45 2805.0 46 2690.0 47 2575.0 48 2523.0 49 2471.0 50 2401.5 51 2332.0 52 2341.0 53 2350.0 54 2845.5 55 3341.0 56 2390.0 57 1439.0 58 1262.0 59 1085.0 60 993.0 61 901.0 62 758.0 63 615.0 64 516.5 65 418.0 66 351.0 67 284.0 68 229.0 69 174.0 70 125.0 71 76.0 72 64.0 73 52.0 74 39.0 75 16.0 76 6.0 77 4.0 78 2.0 79 3.0 80 4.0 81 3.0 82 2.0 83 1.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.045437764461988474 2 0.0014199301394371398 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0014199301394371398 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0014199301394371398 17 0.0 18 0.0014199301394371398 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0014199301394371398 27 0.0 28 0.0 29 0.0 30 0.0028398602788742796 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.004259790418311419 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 70426.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 72.29988426225619 #Duplication Level Percentage of deduplicated Percentage of total 1 90.97852506818754 65.77736832780732 2 6.326225843419249 9.147707925922115 3 1.3352466373411678 2.8961453202399956 4 0.5489290362597342 1.5875002315908242 5 0.23644800270138286 0.8547581614675809 6 0.13134305533679871 0.5697652619700994 7 0.05992827202718905 0.3032964992121941 8 0.05294247994211074 0.30621881378971405 9 0.05255578539127933 0.34197994809913235 >10 0.21189185340556752 3.241919883976743 >50 0.02716323225813868 1.3690928570715122 >100 0.027160513714422074 3.520637136997294 >500 0.005820110007688222 3.033919548435588 >1k 0.005820110007688222 7.04969008341991 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2149 3.051429869650413 No Hit CTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGTC 1503 2.134154999574021 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1374 1.95098401158663 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 843 1.1970011075455087 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 710 1.0081503990003693 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 610 0.8661573850566552 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 301 0.427398971970579 No Hit GCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 251 0.3564024649987221 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 250 0.35498253485928494 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 232 0.32942379234941643 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 219 0.3109647005367336 No Hit TTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGTCT 205 0.29108567858461365 Illumina Paired End PCR Primer 2 (95% over 21bp) GCTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 153 0.21724931133388237 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 150 0.21298952091557094 No Hit GCGTTGATACCACTGCTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 149 0.21156959077613383 No Hit ATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 139 0.1973702893817624 No Hit CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 123 0.17465140715076818 No Hit CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 115 0.16329196603527107 No Hit GCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 114 0.16187203589583393 No Hit GATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 109 0.15477238519864822 No Hit AAAAAGTACTCTGCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAA 89 0.12637378240990543 No Hit AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 89 0.12637378240990543 No Hit GCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 86 0.122113991991594 No Hit CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGAA 79 0.11217448101553404 No Hit ACTCTGCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAA 77 0.10933462073665977 No Hit GATACCACTGCTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 73 0.1036549001789112 No Hit GCGTTGATACCACTGCTTCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 72 0.10223497003947406 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0 0.0 0.0 2 0.0 0.0 0.0 3 0.0 0.0 0.0 4 0.0 0.0 0.0 5 0.0 0.0 0.0 6 0.0 0.0 0.0 7 0.0 0.0 0.0 8 0.0 0.0 0.0 9 0.0 0.0 0.0 10 0.0 0.0 0.0014199301394371396 11 0.0 0.0 0.0014199301394371396 12 0.0 0.0 0.0014199301394371396 13 0.0 0.0 0.0014199301394371396 14 0.0 0.0 0.0014199301394371396 15 0.0 0.0 0.0014199301394371396 16 0.0 0.0 0.0014199301394371396 17 0.0 0.0 0.0014199301394371396 18 0.0 0.0 0.0014199301394371396 19 0.0 0.0 0.002839860278874279 20 0.0 0.0 0.002839860278874279 21 0.0 0.0 0.004259790418311419 22 0.0 0.0 0.012779371254934257 23 0.0 0.0 0.022718882230994233 24 0.0 0.0 0.036918183625365635 25 0.0 0.0 0.03975804390423991 26 0.0 0.0 0.058217135716922726 27 0.0 0.0 0.06673671655354557 28 0.0 0.0 0.09513531934228836 29 0.0 0.0 0.14057308380427683 30 0.0 0.0 0.1959503592423253 31 0.0 0.0 0.3564024649987221 32 0.0 0.0 0.5338937324283646 33 0.0 0.0 0.7085451395791327 34 0.0 0.0 0.9101752193792065 35 0.0 0.0 1.1402039019680232 36 0.0 0.0 1.442649021668134 37 0.0 0.0 1.787692045551359 38 0.0 0.0 2.2094112969641895 39 0.0 0.0 2.6708885922812597 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATCTCGT 180 0.0 33.750004 37 GCCCACG 180 0.0 33.750004 18 CGTATGC 180 0.0 33.750004 41 TCCGAGC 180 0.0 33.750004 13 GTATGCC 180 0.0 33.750004 42 GCGACCT 160 0.0 33.75 30 CGACCTA 160 0.0 33.75 31 GACCTAT 160 0.0 33.75 32 CTCGTAT 185 0.0 32.837837 39 TATGCCG 185 0.0 32.837837 43 TCTCGTA 185 0.0 32.837837 38 CCGAGCC 185 0.0 32.837837 14 TCGTATG 185 0.0 32.837837 40 TGCCGTC 185 0.0 32.837837 45 TGCGACC 165 0.0 32.727272 29 GACTGCG 165 0.0 32.727272 26 TATCTCG 180 0.0 32.500004 36 CGAGACT 180 0.0 32.500004 23 ACCTATC 175 0.0 32.14286 33 ATCTCCG 190 0.0 31.973684 10 >>END_MODULE