FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 36h_33.34100000005da5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 36h_33.34100000005da5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences98142
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7460.7601230869556357No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7300.7438201789244157No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGTC7100.7234415438853905No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4630.47176540115343074No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3550.36172077194269525No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2800.2853008905463512No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1290.1314421960017118No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1210.12329074198610178No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1150.11717715147439424No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1060.10800676570683296No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTAATG207.001746E-445.02
TAGATCT207.001746E-445.04
CGACCTA650.041.53846431
GACCTAT650.041.53846432
ATCTCGT800.039.37537
GCCCACG800.039.37518
CGTATGC750.039.041
CTCGTAT750.039.039
TATGCCG750.039.043
TCTCGTA750.039.038
CGAGCCC750.039.015
TCTCCGA750.039.011
ACGAGAC750.039.022
TGCCGTC750.039.045
TGCGACC700.038.5714329
CGAGACT700.038.5714323
CCCACGA850.037.05882319
CTATCTC800.036.562535
TCCGAGC800.036.562513
ACCTATC800.036.562533
Produced by FastQC (version 0.11.2)