##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename H23NVBCXX l02n01 36h_33.34100000005da5.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 98142 Sequences flagged as poor quality 0 Sequence length 51 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.324723360029346 38.0 38.0 38.0 32.0 38.0 2 36.33777587577184 38.0 38.0 38.0 32.0 38.0 3 36.306596564162135 38.0 38.0 38.0 32.0 38.0 4 36.41242281591979 38.0 38.0 38.0 32.0 38.0 5 36.45698070143262 38.0 38.0 38.0 32.0 38.0 6 38.354048215850504 40.0 38.0 40.0 38.0 40.0 7 38.27357298608139 40.0 38.0 40.0 38.0 40.0 8 38.24103849524159 40.0 38.0 40.0 38.0 40.0 9 38.238470787226674 40.0 38.0 40.0 38.0 40.0 10 38.173442562817144 40.0 38.0 40.0 38.0 40.0 11 38.136322879093555 40.0 38.0 40.0 38.0 40.0 12 38.09873448676408 40.0 38.0 40.0 38.0 40.0 13 38.09324244462106 40.0 38.0 40.0 38.0 40.0 14 38.022284037415176 40.0 38.0 40.0 32.0 40.0 15 38.09091928022661 40.0 38.0 40.0 38.0 40.0 16 38.10957592060484 40.0 38.0 40.0 38.0 40.0 17 38.097990666585154 40.0 38.0 40.0 38.0 40.0 18 38.11371278351776 40.0 38.0 40.0 38.0 40.0 19 38.12414664465774 40.0 38.0 40.0 38.0 40.0 20 38.12967944407084 40.0 38.0 40.0 38.0 40.0 21 38.15210613193128 40.0 38.0 40.0 38.0 40.0 22 38.130677997187746 40.0 38.0 40.0 38.0 40.0 23 38.115862729514376 40.0 38.0 40.0 38.0 40.0 24 38.119327097471015 40.0 38.0 40.0 38.0 40.0 25 38.11412035621854 40.0 38.0 40.0 38.0 40.0 26 38.053228994721934 40.0 38.0 40.0 38.0 40.0 27 38.02218214423998 40.0 38.0 40.0 32.0 40.0 28 37.9837582278739 40.0 38.0 40.0 32.0 40.0 29 37.9594770842249 40.0 38.0 40.0 32.0 40.0 30 37.954830755436 40.0 38.0 40.0 32.0 40.0 31 37.935104236718225 40.0 38.0 40.0 32.0 40.0 32 37.91287114589065 40.0 38.0 40.0 32.0 40.0 33 37.88747936663202 40.0 38.0 40.0 32.0 40.0 34 37.88273114466793 40.0 38.0 40.0 32.0 40.0 35 37.84567259684946 40.0 38.0 40.0 32.0 40.0 36 37.77406207332233 40.0 38.0 40.0 32.0 40.0 37 37.76209981455442 40.0 38.0 40.0 32.0 40.0 38 37.74392207209961 40.0 38.0 40.0 32.0 40.0 39 37.67296366489373 40.0 38.0 40.0 32.0 40.0 40 37.6404189847364 40.0 38.0 40.0 32.0 40.0 41 37.5727415377718 40.0 38.0 40.0 32.0 40.0 42 37.49774816082819 40.0 38.0 40.0 32.0 40.0 43 37.43712172158709 40.0 38.0 40.0 32.0 40.0 44 37.347781785576004 40.0 38.0 40.0 32.0 40.0 45 37.27953373683031 40.0 38.0 40.0 32.0 40.0 46 37.1459008375619 40.0 38.0 40.0 32.0 40.0 47 37.06095249740172 40.0 38.0 40.0 32.0 40.0 48 36.95978276375048 40.0 38.0 40.0 32.0 40.0 49 36.85686046748589 40.0 38.0 40.0 32.0 40.0 50 36.82494752501477 40.0 38.0 40.0 32.0 40.0 51 36.39363371441381 38.0 38.0 40.0 27.0 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2201 1 0.3601996978337141 2201 2 0.47847428467563446 2201 3 -0.24236878553318064 2201 4 0.5669247131504562 2201 5 -0.11017977110702759 2201 6 0.3278223604761408 2201 7 0.17001881765780524 2201 8 0.23425072104425482 2201 9 0.5465005565544914 2201 10 0.4359705475948772 2201 11 0.38908351124371165 2201 12 0.6503340197487546 2201 13 0.4156400243622187 2201 14 0.004766954434380466 2201 15 0.4296193970293487 2201 16 0.4765848741264094 2201 17 -0.13925171294816607 2201 18 0.4658415859436147 2201 19 0.32679728559938326 2201 20 0.541599905567125 2201 21 0.5283902788957988 2201 22 0.1534188970757242 2201 23 -0.08416800539581004 2201 24 -0.14891653401316063 2201 25 0.3091765969930691 2201 26 0.0321571399519911 2201 27 -0.058172458918519965 2201 28 0.11296957584099232 2201 29 0.17631797802407334 2201 30 0.6233918552872737 2201 31 0.15287876942551293 2201 32 0.2619900192926963 2201 33 0.24984010007808877 2201 34 0.34879271086610686 2201 35 0.003478709151494286 2201 36 0.05589618661706197 2201 37 0.14624327001754978 2201 38 0.4556747517668782 2201 39 0.015808090293546684 2201 40 0.19450470495781502 2201 41 -0.253095903960201 2201 42 -0.1981544328262288 2201 43 0.2305631809028128 2201 44 0.6115729013956752 2201 45 0.054740777768969906 2201 46 -0.2103866120585849 2201 47 -0.21412989561371631 2201 48 0.2154116976213487 2201 49 0.21273128384520135 2201 50 0.18469545733777437 2201 51 0.14337193592245256 1101 1 0.7849488617133176 1101 2 0.07820064527155779 1101 3 0.889231498241017 1101 4 0.7769682927592498 1101 5 0.21382955290229688 1101 6 0.47807066289835376 1101 7 0.397544914749119 1101 8 0.2434733824408326 1101 9 -0.1384594110141677 1101 10 0.5947320759216268 1101 11 0.17974936712695921 1101 12 0.3429964298024686 1101 13 0.39613054833100847 1101 14 0.5689201925006557 1101 15 0.45206796295617835 1101 16 0.6508526353506952 1101 17 -0.08254754754835147 1101 18 0.12237345986679316 1101 19 0.05807325600579105 1101 20 0.07124417434182817 1101 21 -0.2299455881357204 1101 22 0.374306704920059 1101 23 0.3883160323056174 1101 24 -0.023802517594795347 1101 25 0.5936602293027846 1101 26 0.6839256612857341 1101 27 0.46528958628265826 1101 28 0.6463637179307895 1101 29 0.18396974654540799 1101 30 0.4402561504124378 1101 31 0.14679789377942143 1101 32 0.5802731854019498 1101 33 0.3041625891831856 1101 34 0.44329965319914066 1101 35 0.6703480716730326 1101 36 0.5054755927182057 1101 37 0.42966274908920354 1101 38 0.849360775887682 1101 39 1.0871063572439894 1101 40 1.155587100822821 1101 41 1.0823657358492653 1101 42 0.9117573944769006 1101 43 1.1320023214723847 1101 44 0.651503984805025 1101 45 1.0882362677861934 1101 46 0.9271931994342637 1101 47 0.6687625742352736 1101 48 0.9737475646528679 1101 49 0.757071002097959 1101 50 0.5839556174675025 1101 51 0.4769586390743754 2202 1 0.5319694467620693 2202 2 0.3419158033529115 2202 3 0.5383007388595544 2202 4 0.2220584139057138 2202 5 0.5564868955596367 2202 6 0.32914916186418 2202 7 0.5786736451736232 2202 8 0.7473146116341738 2202 9 0.4960821038090373 2202 10 0.24838124365530234 2202 11 0.03452135570176296 2202 12 0.7759719050314686 2202 13 0.44921830564411636 2202 14 0.16490167580608528 2202 15 0.4209441571844863 2202 16 -0.10414588725346619 2202 17 0.037416994951406934 2202 18 0.3285686731042574 2202 19 0.4398815885183467 2202 20 0.5513978235095607 2202 21 0.6216746226394179 2202 22 -0.14327430561870358 2202 23 0.6598613883580171 2202 24 0.7166682792140264 2202 25 0.5848451007693498 2202 26 0.7882298078434005 2202 27 0.08185611834214512 2202 28 0.1652251382006611 2202 29 -0.018925949308034262 2202 30 0.781485343754305 2202 31 0.5423460076373985 2202 32 0.6198181474821283 2202 33 0.5809280772162779 2202 34 0.5376067545484915 2202 35 0.6111537448730715 2202 36 0.5434446658985479 2202 37 0.5872516390724556 2202 38 0.8615739148613883 2202 39 0.20564275042826807 2202 40 0.5138555928941315 2202 41 0.7777267129003818 2202 42 0.4439153773391098 2202 43 0.5173564040095684 2202 44 0.20148921084658866 2202 45 0.6479230598673524 2202 46 0.5694664191722723 2202 47 1.0901158688280006 2202 48 0.3826907341594179 2202 49 0.7061993385502419 2202 50 1.0528318116958104 2202 51 1.124796716489918 1102 1 -0.1361300593656054 1102 2 -0.09662452955361545 1102 3 0.0071136161231351025 1102 4 -0.15959514380418227 1102 5 0.19285053192326984 1102 6 -0.16728398245628995 1102 7 -0.017540000335799277 1102 8 0.15905779802524478 1102 9 0.6527493801946207 1102 10 0.23109571228525994 1102 11 0.13978932716691617 1102 12 0.21112829793433718 1102 13 0.23429238649284656 1102 14 0.08890021248068081 1102 15 0.20281721370542982 1102 16 0.44505842955648944 1102 17 0.18118871618791133 1102 18 0.23226356975690265 1102 19 0.2244069223394547 1102 20 0.7011143042119627 1102 21 0.5542701960800613 1102 22 0.3578231884365408 1102 23 0.38731703130661543 1102 24 -0.19662969042196465 1102 25 -0.05069541505285713 1102 26 0.32728230464237384 1102 27 0.1595952805883485 1102 28 0.44206801363508674 1102 29 0.384768947344611 1102 30 0.7519444621007523 1102 31 0.03091377789530725 1102 32 0.25060285573162133 1102 33 -0.17835489333429422 1102 34 -2.567903573051922E-4 1102 35 -0.3656159642910026 1102 36 0.06841265565526555 1102 37 0.24035205977850893 1102 38 -0.30048937396246345 1102 39 -0.1541523840147505 1102 40 -0.12113617590045322 1102 41 0.055392708876240704 1102 42 -0.07026058754107822 1102 43 0.22341091288097914 1102 44 0.2289265622275991 1102 45 -0.6405349609850362 1102 46 -0.3145650423239772 1102 47 -0.3906779852052864 1102 48 -0.5442344533291461 1102 49 -0.6749969299699714 1102 50 -0.8341263006144146 1102 51 0.1527828148985506 2203 1 -0.0781811117614879 2203 2 0.33409647358117667 2203 3 0.10760622696057709 2203 4 0.2569297106517041 2203 5 0.09441793004239685 2203 6 -0.13369687990367396 2203 7 0.18071347033146878 2203 8 -0.26060185266888425 2203 9 -0.5468027917713414 2203 10 -0.2510359491567442 2203 11 0.02971319640113279 2203 12 0.7363410162505062 2203 13 0.29904832021429684 2203 14 0.049894390716232806 2203 15 -0.1973171347047824 2203 16 -0.042805430721166715 2203 17 0.5100736243831676 2203 18 0.3461014560085829 2203 19 0.18611762542946764 2203 20 0.1859777166615828 2203 21 0.14343275765641295 2203 22 -0.08091393650748557 2203 23 0.23577202458919544 2203 24 0.1396891631382644 2203 25 -0.016660484466200387 2203 26 -0.12036659817893991 2203 27 -0.03198555237179335 2203 28 0.17493859133324463 2203 29 0.31929648876870687 2203 30 -0.1729099937881884 2203 31 0.5306898638782869 2203 32 0.17868167346561137 2203 33 0.058635702276987445 2203 34 0.024105054694196326 2203 35 -0.2344023503187671 2203 36 0.23110858042015536 2203 37 0.03824726801854439 2203 38 0.12888814507022772 2203 39 0.2963178354209859 2203 40 0.15732329566246506 2203 41 0.010072511831907605 2203 42 -0.1650209995428753 2203 43 0.23930880808921984 2203 44 -0.7567429406832815 2203 45 -0.48383993187621144 2203 46 -0.06181089991466138 2203 47 -0.3771983613808345 2203 48 -0.22032043631168108 2203 49 -0.15018725688443624 2203 50 -0.5412915491589771 2203 51 -0.5091518022084784 1103 1 0.5424206892488357 1103 2 0.15931061810128 1103 3 -0.0620401600370073 1103 4 0.07735580333784497 1103 5 0.21890727772524343 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7.0 21 16.0 22 22.0 23 74.0 24 147.0 25 256.0 26 386.0 27 623.0 28 848.0 29 1171.0 30 1409.0 31 1795.0 32 2149.0 33 2783.0 34 3386.0 35 4354.0 36 5791.0 37 8381.0 38 15051.0 39 49479.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.50558075531319 19.96636257071505 13.630294072677234 25.897762601294534 2 14.819645404524149 22.14897085795802 39.050336254330546 23.981047483187286 3 19.406574147663587 24.35552566689083 29.85979499093151 26.378105194514074 4 13.55790589146339 18.501762751930876 35.53422591754804 32.40610543905769 5 14.187605714169266 36.73147072609076 33.716451672067 15.364471887672964 6 31.490085794053513 38.142691202543254 15.911638238470788 14.455584764932444 7 26.62774347374213 33.24264840740967 20.510077235026795 19.6195308838214 8 24.36265818915449 36.782417313688335 19.782559964133604 19.072364533023578 9 24.677508100507428 18.234802632919646 20.847343644922663 36.24034562165026 10 15.839294084082248 30.159360926005174 31.205803835259115 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30.9469951702635 21.29261682052536 24 20.898290232520225 27.479570418373378 31.66432312363718 19.95781622546922 25 20.42244910435899 28.16429255568462 31.230258197305943 20.183000142650446 26 20.319536997411912 28.039982881946568 31.722402233498396 19.918077887143117 27 20.3490860182185 28.427176947688043 31.25165576409692 19.972081269996536 28 19.914002160135315 27.730227629353386 31.006093211876667 21.34967699863463 29 20.555929163864604 27.778117421695093 31.402457663385707 20.263495751054595 30 20.876131083394473 27.70542920029347 31.210768729110622 20.207670987201436 31 20.81066210185242 27.350166085875564 30.668826801980803 21.17034501029121 32 20.714882517169002 28.18161439546779 30.713659799066654 20.38984328829655 33 20.060728332416293 28.663569114140735 31.020358256403984 20.255344297038985 34 20.270628273318252 27.9869984308451 31.183387336716184 20.558985959120456 35 20.367426789753623 28.08583481078437 30.619918077887142 20.92682032157486 36 19.931323999918487 28.257015345112187 31.709156120723037 20.102504534246297 37 20.585478184671192 27.990055226100957 30.52719528845958 20.897271300768274 38 20.066841922928003 28.154103238165106 31.410609117401318 20.368445721505573 39 20.56713741313607 27.53153593772289 30.555725377514214 21.345601271626826 40 21.238613437671944 27.17490982453995 31.302602351694485 20.28387438609362 41 20.928858185078763 27.15860691650873 30.653542825701535 21.25899207271097 42 21.327260500091704 27.479570418373378 30.33156039208494 20.86160868944998 43 20.29915836237289 27.509119439179962 31.140592203134233 21.051129995312916 44 19.947626907949704 28.53925944040268 30.312200688797862 21.20091296284975 45 20.232927798496057 27.636485908173867 30.796193270974708 21.334393022355364 46 21.099019787654623 27.364431130402885 30.036070184019074 21.50047889792342 47 20.56102382262436 27.451040329318744 29.51947178577979 22.468464062277107 48 20.21968168572069 27.758757718408024 29.47056306168613 22.55099753418516 49 21.56874732530415 27.616107273134844 29.157751013837093 21.65739438772391 50 20.144280736076297 28.456725968494627 29.553096533594182 21.845896761834894 51 20.219280815985492 28.122357064979266 29.165775074129552 22.492587044905697 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 755.0 1 429.0 2 103.0 3 139.5 4 176.0 5 209.0 6 242.0 7 229.0 8 216.0 9 236.5 10 257.0 11 416.5 12 576.0 13 841.5 14 1107.0 15 1258.5 16 1410.0 17 1267.5 18 1125.0 19 1033.5 20 942.0 21 970.0 22 998.0 23 963.5 24 929.0 25 999.0 26 1292.0 27 1515.0 28 1752.0 29 1989.0 30 2502.0 31 3015.0 32 3524.0 33 4033.0 34 4521.5 35 5010.0 36 5231.5 37 5453.0 38 5513.5 39 5574.0 40 5727.0 41 5880.0 42 5992.5 43 6105.0 44 6200.0 45 6295.0 46 6317.5 47 6340.0 48 6278.0 49 6216.0 50 6050.0 51 5884.0 52 5828.5 53 5773.0 54 5670.0 55 5567.0 56 4705.5 57 3844.0 58 3420.5 59 2997.0 60 2681.5 61 2366.0 62 2000.0 63 1634.0 64 1387.0 65 1140.0 66 915.5 67 691.0 68 564.0 69 437.0 70 320.5 71 204.0 72 170.5 73 137.0 74 111.5 75 60.0 76 34.0 77 22.0 78 10.0 79 8.0 80 6.0 81 4.0 82 2.0 83 1.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03770047482219641 2 0.0020378635039025087 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.003056795255853763 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.003056795255853763 18 0.0020378635039025087 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.006113590511707526 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.003056795255853763 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 98142.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 83.79667669114642 #Duplication Level Percentage of deduplicated Percentage of total 1 90.44770152863495 75.7921680245233 2 7.377888881972473 12.364851386117019 3 1.276665638030949 3.2094101313832675 4 0.42374437980815954 1.4203348317789877 5 0.17345917647363499 0.7267651265036849 6 0.11010560245334272 0.5535890142399994 7 0.0388053837699088 0.22762335383500354 8 0.02827624903653707 0.18955645588424294 9 0.028325330115253594 0.21362116768541087 >10 0.07679788240169184 1.1656824356645672 >50 0.006096702083081228 0.3186267893509172 >100 0.008493317073615666 1.5952367957132558 >500 0.0036399281464362244 2.222534487320338 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 746 0.7601230869556357 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 730 0.7438201789244157 No Hit CTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGTC 710 0.7234415438853905 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 463 0.47176540115343074 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 355 0.36172077194269525 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 280 0.2853008905463512 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 129 0.1314421960017118 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 121 0.12329074198610178 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 115 0.11717715147439424 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 106 0.10800676570683296 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0 0.0 0.0 2 0.0 0.0 0.0 3 0.0 0.0 0.0 4 0.0 0.0 0.0 5 0.0 0.0 0.0 6 0.0 0.0 0.0 7 0.0 0.0 0.0 8 0.0 0.0 0.0 9 0.0 0.0 0.0 10 0.0 0.0 0.0 11 0.0 0.0 0.0010189317519512543 12 0.0 0.0 0.0010189317519512543 13 0.0 0.0 0.0020378635039025087 14 0.0 0.0 0.0020378635039025087 15 0.0 0.0 0.0020378635039025087 16 0.0 0.0 0.0020378635039025087 17 0.0 0.0 0.0020378635039025087 18 0.0 0.0 0.0020378635039025087 19 0.0 0.0 0.0020378635039025087 20 0.0 0.0 0.0020378635039025087 21 0.0 0.0 0.003056795255853763 22 0.0 0.0 0.003056795255853763 23 0.0 0.0 0.00713252226365878 24 0.0 0.0 0.009170385767561288 25 0.0 0.0 0.009170385767561288 26 0.0 0.0 0.012227181023415051 27 0.0 0.0 0.018340771535122577 28 0.0 0.0 0.029549020806586376 29 0.0 0.0 0.0468708605897577 30 0.0 0.0 0.0825334719080516 31 0.0 0.0 0.15997228505634692 32 0.0 0.0 0.28631982229830244 33 0.0 0.0 0.416743086548063 34 0.0 0.0 0.5481852825497748 35 0.0 0.0 0.7193658168775855 36 0.0 0.0 0.9476065293146665 37 0.0 0.0 1.2267938293493101 38 0.0 0.0 1.5915713965478593 39 0.0 0.0 1.9848790528010434 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCTAATG 20 7.001746E-4 45.0 2 TAGATCT 20 7.001746E-4 45.0 4 CGACCTA 65 0.0 41.538464 31 GACCTAT 65 0.0 41.538464 32 ATCTCGT 80 0.0 39.375 37 GCCCACG 80 0.0 39.375 18 CGTATGC 75 0.0 39.0 41 CTCGTAT 75 0.0 39.0 39 TATGCCG 75 0.0 39.0 43 TCTCGTA 75 0.0 39.0 38 CGAGCCC 75 0.0 39.0 15 TCTCCGA 75 0.0 39.0 11 ACGAGAC 75 0.0 39.0 22 TGCCGTC 75 0.0 39.0 45 TGCGACC 70 0.0 38.57143 29 CGAGACT 70 0.0 38.57143 23 CCCACGA 85 0.0 37.058823 19 CTATCTC 80 0.0 36.5625 35 TCCGAGC 80 0.0 36.5625 13 ACCTATC 80 0.0 36.5625 33 >>END_MODULE