FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 36h_32.34100000005d98.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 36h_32.34100000005d98.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55096
Sequences flagged as poor quality0
Sequence length51
%GC31

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTTGCAGAATCTCGTATGCCGTC42817.770074052562799No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13272.408523304777116No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9051.6425874836648757No Hit
GCGTTGATACCACTGCTTCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8421.5282416146362712No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6121.110788441992159No Hit
CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6101.1071584144039495No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5440.9873675039930304No Hit
TTATACACATCTCCGAGCCCACGAGACCTTGCAGAATCTCGTATGCCGTCT5310.9637723246696674RNA PCR Primer, Index 40 (95% over 21bp)
AAAAAGTACTCTGCGTTGATACCACTGCTTCGAAAAAAAAAAAAAAAAAAA5130.9311020763757805No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4600.8349063452882243No Hit
AAAGTACTCTGCGTTGATACCACTGCTTCGAAAAAAAAAAAAAAAAAAAAA3990.7241905038478292No Hit
GCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3600.6534049658777407No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3300.5989545520545956No Hit
GCTTCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3130.5680993175548134No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT3010.5463191520255554No Hit
ACTCTGCGTTGATACCACTGCTTCGAAAAAAAAAAAAAAAAAAAAAAAAAA3000.5445041382314506No Hit
GCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3000.5445041382314506No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAA2990.5426891244373457No Hit
GATACCACTGCTTCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2920.5299840278786119No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT2840.5154639175257731No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2390.43378829679105557No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAA2370.43015826920284594No Hit
AAAGTACTCTGCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAA2210.4011180484971686No Hit
CCACTGCTTCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2140.3884129519384347No Hit
TGATACCACTGCTTCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2120.38478292435022504No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2070.37570785537970086No Hit
AAAGTACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAA1790.3248874691447655No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1620.29403223464498335No Hit
GCGTTGATACCACTGCTTGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1610.2922172208508785No Hit
ACTCTGCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAA1520.27588209670393493No Hit
ACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAAAAAAA1490.27043705532162043No Hit
ATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1480.2686220415275156No Hit
GTTGATACCACTGCTTCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1330.24139683461594308No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGAA1270.23050675185131408No Hit
ATACCACTGCTTCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1260.22869173805720924No Hit
TACCACTGCTTCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1240.22506171046899956No Hit
AGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1190.21598664149847538No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTGGCAAAAAAAAAAAAAAAAAA1080.1960214897633222No Hit
GTACTCTGCGTTGATACCACTGCTTCGAAAAAAAAAAAAAAAAAAAAAAAA1040.18876143458690287No Hit
CCACTGCTTCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1000.18150137941048353No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT960.1742413242340642No Hit
AAAGTACTCTGCGTTGATACCACTGCTTGGCAAAAAAAAAAAAAAAAAAAA940.1706112966458545No Hit
AAAAAAGTACTCTGCGTTGATACCACTGCTTCGAAAAAAAAAAAAAAAAAA930.16879628285174966No Hit
TACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA910.16516625526354No Hit
ATACACATCTCCGAGCCCACGAGACCTTGCAGAATCTCGTATGCCGTCTTC850.15427617249891099RNA PCR Primer, Index 40 (100% over 22bp)
GATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA830.15064614491070133No Hit
GCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA820.1488311311165965No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCAT820.1488311311165965No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGCC800.1452011035283868No Hit
TTTCGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT790.14338608973428196No Hit
GAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGAAACATCCACTTGA790.14338608973428196No Hit
GATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA760.13794104835196747No Hit
TGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA740.1343110207637578No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT720.13068099317554813No Hit
GTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA710.12886597938144329No Hit
AAAAAAAAGTACTCTGCGTTGATACCACTGCTTCGAAAAAAAAAAAAAAAA700.12705096558733847No Hit
ACTCTGCGTTGATACCACTGCTTGGCAAAAAAAAAAAAAAAAAAAAAAAAA670.12160592420502397No Hit
CTCTGCGTTGATACCACTGCTTCGAAAAAAAAAAAAAAAAAAAAAAAAAAA670.12160592420502397No Hit
CTTATACACATCTCCGAGCCCACGAGACGTCTTAGTATCTCGTATGCCGTC650.11797589661681428No Hit
GTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA640.11616088282270945No Hit
TGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA590.10708581385218527No Hit
GCAGTGGACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA580.10527080005808044No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT570.1034557862639756No Hit
GCTTCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA560.10164077246987077No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCTCG351.1839802E-745.00000436
TGCCGTC5100.037.94117745
TCGTATG5050.037.8712940
CTCGTAT5000.037.839
ATGCCGT5150.037.5728244
TATGCCG5100.037.543
ATCTCGT5050.037.42574337
TCTCGTA5050.037.42574338
AATCTCG4700.037.34042736
GAATCTC4700.037.34042735
CGTATGC5150.037.13592541
CCTTGCA4850.037.11340328
CTTGCAG4850.037.11340329
AGACCTT4850.037.11340325
GACCTTG4850.037.11340326
GAGACCT4850.037.11340324
TTGCAGA4800.037.03124630
GCAGAAT4800.037.03124632
TGCAGAA4800.037.03124631
AGAATCT4750.036.9473734
Produced by FastQC (version 0.11.2)