##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename H23NVBCXX l02n01 36h_31.34100000005d8b.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 20061 Sequences flagged as poor quality 0 Sequence length 51 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.0611136035093 38.0 38.0 38.0 32.0 38.0 2 36.29036438861473 38.0 38.0 38.0 32.0 38.0 3 36.159812571656445 38.0 38.0 38.0 32.0 38.0 4 36.34674243557151 38.0 38.0 38.0 32.0 38.0 5 36.42291012412143 38.0 38.0 38.0 32.0 38.0 6 38.47769303623947 40.0 38.0 40.0 38.0 40.0 7 38.116345147300734 40.0 38.0 40.0 38.0 40.0 8 38.06385524151339 40.0 38.0 40.0 38.0 40.0 9 38.2304969841982 40.0 38.0 40.0 38.0 40.0 10 38.07507103334829 40.0 38.0 40.0 32.0 40.0 11 38.14007277802702 40.0 38.0 40.0 38.0 40.0 12 37.69423259059867 40.0 38.0 40.0 32.0 40.0 13 37.9799112706246 40.0 38.0 40.0 32.0 40.0 14 37.80090723293954 40.0 38.0 40.0 32.0 40.0 15 37.73505807287772 40.0 38.0 40.0 32.0 40.0 16 37.373610488011565 40.0 38.0 40.0 32.0 40.0 17 37.261602113553664 40.0 38.0 40.0 32.0 40.0 18 37.257614276456806 40.0 38.0 40.0 32.0 40.0 19 37.38781715766911 40.0 38.0 40.0 32.0 40.0 20 37.566621803499324 40.0 38.0 40.0 32.0 40.0 21 37.80943123473406 40.0 38.0 40.0 32.0 40.0 22 37.888639649070335 40.0 38.0 40.0 32.0 40.0 23 37.90613628433279 40.0 38.0 40.0 32.0 40.0 24 37.71372314440955 40.0 38.0 40.0 32.0 40.0 25 37.350530880813515 40.0 38.0 40.0 32.0 40.0 26 37.32052240665969 40.0 38.0 40.0 32.0 40.0 27 37.34848711430138 40.0 38.0 40.0 32.0 40.0 28 37.34105976770849 40.0 38.0 40.0 32.0 40.0 29 37.39703903095558 40.0 38.0 40.0 32.0 40.0 30 37.437415881561236 38.0 38.0 40.0 32.0 40.0 31 37.463536214545634 40.0 38.0 40.0 32.0 40.0 32 37.197497632221726 40.0 38.0 40.0 32.0 40.0 33 37.127511091171925 40.0 38.0 40.0 32.0 40.0 34 37.21524350730272 40.0 38.0 40.0 32.0 40.0 35 36.999152584616915 40.0 38.0 40.0 32.0 40.0 36 36.79587258860476 40.0 38.0 40.0 32.0 40.0 37 36.836747918847514 40.0 38.0 40.0 32.0 40.0 38 36.93225661731718 40.0 38.0 40.0 32.0 40.0 39 36.5468820098699 38.0 38.0 40.0 32.0 40.0 40 36.49484073575594 38.0 38.0 40.0 27.0 40.0 41 36.6243457454763 38.0 38.0 40.0 32.0 40.0 42 36.588455211604604 38.0 38.0 40.0 32.0 40.0 43 36.89751258661084 40.0 38.0 40.0 32.0 40.0 44 36.63675788844026 38.0 38.0 40.0 27.0 40.0 45 36.65714570559793 38.0 38.0 40.0 32.0 40.0 46 36.1913164847216 38.0 38.0 40.0 27.0 40.0 47 36.241513384178255 38.0 38.0 40.0 27.0 40.0 48 36.13070136084941 38.0 38.0 40.0 27.0 40.0 49 35.896316235481784 38.0 38.0 40.0 27.0 40.0 50 35.72214745027666 38.0 38.0 40.0 27.0 40.0 51 35.113852749115196 38.0 32.0 40.0 27.0 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2201 1 -0.48979737164205517 2201 2 0.8524311835695499 2201 3 -0.0734958303514972 2201 4 -0.3430012981437116 2201 5 -0.3316822363018588 2201 6 0.010491198256353584 2201 7 0.09201137420559036 2201 8 0.6201615050518114 2201 9 0.5904008719429683 2201 10 0.28349185140233857 2201 11 1.1141394127982736 2201 12 -0.1379673062728699 2201 13 0.06804357084400436 2201 14 -1.3300699336634665 2201 15 0.6946577393125537 2201 16 0.62687011147883 2201 17 1.3893169777466312 2201 18 -0.03950965738519585 2201 19 0.7967163147538798 2201 20 0.9053441349708109 2201 21 1.3655555105943051 2201 22 0.38546284047762214 2201 23 1.2040302793157451 2201 24 0.1646961331270802 2201 25 0.8036109378185259 2201 26 0.5066251625307103 2201 27 -0.6339886988348127 2201 28 0.9692894280332993 2201 29 -0.8370529420891089 2201 30 -0.4187476196954236 2201 31 -1.5252536286834086 2201 32 0.021958273268630535 2201 33 1.1290867769230033 2201 34 0.16173408837094172 2201 35 -0.32201282440266965 2201 36 -0.8164907351237005 2201 37 0.3838806787601712 2201 38 0.06679205113206166 2201 39 0.5980460736986544 2201 40 0.2669824203483415 2201 41 0.1504535756812686 2201 42 0.039098579196213734 2201 43 0.02600493003486548 2201 44 0.5249617397659634 2201 45 0.6481963642769983 2201 46 1.5640269367951376 2201 47 1.4257627146766438 2201 48 0.9341134896056431 2201 49 1.431248780783612 2201 50 1.0763717609407308 2201 51 1.9794035533669856 1101 1 1.2006788188341346 1101 2 -0.29042595928759596 1101 3 -0.1806386874943513 1101 4 1.1054114002689914 1101 5 0.45006379544417285 1101 6 0.9152531030182587 1101 7 1.3618526440468628 1101 8 -0.49888611399580896 1101 9 -0.5656837841416902 1101 10 -0.14375682584633864 1101 11 1.0109648096236725 1101 12 -0.26495143325699644 1101 13 1.2201599729604098 1101 14 1.1328930292994954 1101 15 0.5610598557146673 1101 16 -0.03715105254233464 1101 17 0.26762385605351113 1101 18 0.8626067447312096 1101 19 1.1379861560237217 1101 20 0.6698943995210698 1101 21 1.4131745582133561 1101 22 0.6182670732818565 1101 23 0.6908027660882254 1101 24 0.6422093606403081 1101 25 0.4795368637444497 1101 26 1.3426039985095457 1101 27 0.8805615657154462 1101 28 -0.6087529000090299 1101 29 1.518767163730999 1101 30 -0.12112857207637262 1101 31 1.1705135670837876 1101 32 1.0669318182421748 1101 33 0.5153301631663894 1101 34 1.4408346174714737 1101 35 -0.5191027714926193 1101 36 1.9268955082625467 1101 37 -0.051304506425012164 1101 38 -0.22950424516423595 1101 39 0.2382577139102935 1101 40 1.220686124052044 1101 41 0.8065382317659271 1101 42 -1.382859092761457 1101 43 0.13447053850047297 1101 44 0.976019940824159 1101 45 0.4405244066050358 1101 46 -0.6264492536810522 1101 47 -0.8096870207730973 1101 48 -1.0645637590716035 1101 49 -1.029068679533843 1101 50 -0.36807268350371203 1101 51 -0.26662819266475424 2202 1 0.5340121521674632 2202 2 0.5984629296012898 2202 3 1.1526946458389844 2202 4 -0.4871811923236038 2202 5 0.45006379544417285 2202 6 1.2115493993145492 2202 7 -0.15666587447165625 2202 8 -0.017404632514328 2202 9 0.5824643640064622 2202 10 -0.291904973994491 2202 11 0.6035574022162606 2202 12 1.1054189371133774 2202 13 -0.002062249261811644 2202 14 -0.015255118848649829 2202 15 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18 0.746204628329096 1215 19 0.5538591718967325 1215 20 -0.10894157931490867 1215 21 0.7655555105943037 1215 22 0.3283199833347652 1215 23 -0.08168400639854667 1215 24 -0.24244672401577816 1215 25 0.6107537949613828 1215 26 0.3066251625307146 1215 27 1.0445827297366108 1215 28 -1.3664248576809896 1215 29 -0.358481513517674 1215 30 -0.1401761911239916 1215 31 0.23188922845945115 1215 32 1.036243987554343 1215 33 -0.7566275087912828 1215 34 -0.4311230544861999 1215 35 -0.42915568154553085 1215 36 -0.08791930655227276 1215 37 -1.5232621783826872 1215 38 -0.6189222345822216 1215 39 0.3409032165558017 1215 40 -0.42587472250880154 1215 41 -0.9352607100330204 1215 42 -0.4537585636609265 1215 43 0.1260049300348669 1215 44 0.17496173976596197 1215 45 -0.8875179214372935 1215 46 -2.1216873489191457 1215 47 0.21147700039092854 1215 48 -0.8158865103943569 1215 49 -1.1116083620735253 1215 50 0.07637176094073084 1215 51 -2.1777393037758657 1212 1 0.7383132274362794 1212 2 0.35473533103498767 1212 3 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0.47362211056501735 1212 40 -0.1281788699742421 1212 41 -1.176735364410895 1212 42 -0.2777216973014802 1212 43 -0.870308434020437 1212 44 -0.589093559773211 1212 45 -0.8736930366446671 1212 46 -0.3253739848638446 1212 47 0.1377442814969143 1212 48 0.6080766232461983 1212 49 -1.0111475325804378 1212 50 0.30217821255363475 1212 51 -0.6680618844210287 1213 1 -0.37589775774244316 1213 2 -0.2363719052335398 1213 3 -0.6130711199267864 1213 4 0.09039638525397464 1213 5 -0.06044671506633392 1213 6 0.014352202117358104 1213 7 -0.5180272358330171 1213 8 -0.13852575363544872 1213 9 -1.8169350353929374 1213 10 -0.5051181872076995 1213 11 -0.5325787339198769 1213 12 -0.7364229047284638 1213 13 0.44738720018763445 1213 14 -1.5597996633931928 1213 15 -1.1306318359770273 1213 16 -0.1742881896794657 1213 17 -1.1628065743769227 1213 18 -1.3425984604739938 1213 19 -1.1472991292615617 1213 20 -1.8456211159944402 1213 21 -0.4336722886334954 1213 22 0.28893774395252336 1213 23 -0.4523403770549166 1213 24 -0.5659988475679043 1213 25 -2.4193620351544496 1213 26 -2.7134520575465046 1213 27 -0.7971161119622323 1213 28 -0.15329744455357286 1213 29 -0.8496012046373664 1213 30 -0.8868943378421434 1213 31 -0.14880575223553194 1213 32 -1.3815166302062707 1213 33 -0.32651167867545183 1213 34 -0.9685747919379395 1213 35 -0.4330166854065354 1213 36 -0.9690003876333506 1213 37 0.18793473281422735 1213 38 0.4992244835644968 1213 39 -0.5525330768804935 1213 40 -1.6181527147867953 1213 41 0.5983300235577147 1213 42 0.02655031664795615 1213 43 0.7460821501120805 1213 44 -0.8698259050216848 1213 45 -0.2581742920936634 1213 46 0.11228948505768699 1213 47 -0.27114848223455823 1213 48 0.8173181228102777 1213 49 0.400360749895583 1213 50 0.130425814994787 1213 51 -0.6480095740461422 >>END_MODULE >>Per sequence quality scores pass #Quality Count 16 1.0 17 1.0 18 0.0 19 1.0 20 5.0 21 8.0 22 12.0 23 27.0 24 54.0 25 68.0 26 124.0 27 183.0 28 269.0 29 363.0 30 470.0 31 510.0 32 651.0 33 670.0 34 830.0 35 1002.0 36 1280.0 37 1855.0 38 3127.0 39 8550.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 14.203072012766807 17.240175543586673 13.614602034709755 54.94215040893676 2 4.5860126613827825 18.204476347141217 66.47724440456606 10.732266586909924 3 9.102238173570608 22.007875978266288 63.03773490852899 5.852150939634116 4 6.83415582473456 62.723692737151694 21.74368177059967 8.698469667514082 5 4.127411395244504 26.155226558995064 63.63092567668611 6.086436369074323 6 13.957429838991079 67.36952295498729 10.502965953840786 8.170081252180848 7 5.7923333831812975 22.984896066995663 16.4498280245252 54.77294252529784 8 8.509047405413488 66.62678829569812 9.750261701809482 15.11390259707891 9 9.520961068740343 16.90344449429241 9.501021883256069 64.07457255371118 10 6.106375554558596 60.425701610089234 19.311101141518368 14.156821693833807 11 9.800109665520164 16.130801056776832 55.78984098499576 18.279248292707244 12 9.221873286476248 12.905637804695678 22.282039778675042 55.59044913015303 13 14.690194905538108 8.543940980010966 58.97512586610837 17.790738248342556 14 9.520961068740343 11.818952195802801 15.393051193858732 63.267035541598126 15 17.74587508100294 10.762175365136335 13.24460395792832 58.24734559593241 16 55.35117890434176 16.39499526444345 13.638402871242711 14.615422959972085 17 16.435692921236292 60.33399800598206 14.64606181455633 8.584247258225323 18 59.29710867397806 13.76370887337986 16.385842472582254 10.55333998005982 19 11.016399980060815 20.118638153631423 13.12496884502268 55.73999302128508 20 8.887891929614675 11.126065500224316 17.86052539753751 62.1255171726235 21 5.169233836797766 19.799611185883055 13.902597078909325 61.12855789840985 22 6.226010667464234 60.92418124719605 14.062110562783511 18.787697522556204 23 7.821145506206071 15.637306216041075 15.273416080953092 61.26813219679976 24 51.921639001046806 20.706844125417476 16.170679427745377 11.20083744579034 25 14.625392552714223 58.6411445092468 14.211654453915557 12.521808484123422 26 53.52674343253078 19.171526843128458 17.815662230197894 9.486067494142866 27 11.943572105079507 60.430686406460296 16.689098250336475 10.936643238123722 28 7.4971337420866355 15.009221873286476 16.504660784606948 60.98898360001994 29 6.8989581775584465 12.476945316783809 21.369822042769552 59.25427446288819 30 6.5902293120638085 11.660019940179462 66.38584247258225 15.363908275174476 31 10.911719256268382 10.951597627236927 66.90095209610686 11.235731020387817 32 52.878719904291906 12.761078709934697 21.868301679876375 12.491899705897014 33 11.225761427645681 15.7120781616071 18.54344250037386 54.51871791037336 34 10.303574098998055 59.58825581974976 16.434873635411993 13.673296445840188 35 51.597627236927366 21.589153083096555 14.809830018443746 12.003389661532326 36 10.00947111310503 64.06460296096904 17.102836349135135 8.823089576790789 37 6.649718359005035 66.16818702955985 17.367030556801755 9.815064054633368 38 5.548078360998953 20.0438662080654 62.060714819799614 12.347340611136035 39 5.862120532376252 15.682169383380689 23.463436518618213 54.99227356562485 40 5.323762524300882 13.743083595035143 62.74861671900703 18.184537161656948 41 8.419321070734261 13.105029659538408 19.699915258461694 58.775734011265634 42 51.5627336623299 13.35426947809182 20.537361048801156 14.54563581077713 43 9.15707093365236 15.457853546682617 60.41573201734709 14.96934350231793 44 7.108319625143313 59.24928966651712 23.59304122426599 10.049349484073575 45 7.975674193709187 16.479736802751606 63.13244603957928 12.412142963959923 46 52.85379592243656 12.965455361148498 18.68800159513484 15.492747121280095 47 11.056278351029361 10.732266586909924 16.883505308808136 61.327949753252575 48 9.58077862519316 11.435122875230547 12.81591147001645 66.16818702955985 49 53.45197148696476 10.607646677633218 16.778824585015702 19.16155725038632 50 9.815064054633368 12.432082149444195 59.44369672498878 18.30915707093365 51 6.8590798065899 11.425153282488411 19.480584218134688 62.23518269278699 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 99.0 1 61.5 2 24.0 3 21.5 4 19.0 5 23.0 6 27.0 7 19.0 8 11.0 9 20.0 10 29.0 11 42.5 12 56.0 13 59.5 14 63.0 15 102.5 16 142.0 17 101.5 18 61.0 19 47.0 20 33.0 21 39.0 22 45.0 23 43.0 24 41.0 25 39.5 26 53.0 27 68.0 28 96.5 29 125.0 30 181.0 31 237.0 32 358.0 33 479.0 34 788.0 35 1097.0 36 1225.0 37 1353.0 38 1286.5 39 1220.0 40 1136.0 41 1052.0 42 920.0 43 788.0 44 783.5 45 779.0 46 573.0 47 367.0 48 319.0 49 271.0 50 1026.0 51 1781.0 52 5645.0 53 9509.0 54 4829.0 55 149.0 56 92.0 57 35.0 58 25.0 59 15.0 60 14.5 61 14.0 62 12.0 63 10.0 64 8.5 65 7.0 66 7.5 67 8.0 68 6.5 69 5.0 70 2.5 71 0.0 72 1.5 73 3.0 74 1.5 75 0.0 76 0.0 77 0.5 78 1.0 79 0.5 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04486316733961417 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.004984796371068242 18 0.004984796371068242 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.004984796371068242 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 20061.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 24.62987886944818 #Duplication Level Percentage of deduplicated Percentage of total 1 76.82655332928557 18.922287024575045 2 11.39445456385347 5.6128807138228405 3 4.573972879983809 3.379691939584268 4 2.206031167779802 2.1733712177857534 5 1.2345679012345678 1.5203628931758137 6 0.44525399716656544 0.6579931209810079 7 0.44525399716656544 0.7676586411445092 8 0.42501517911353975 0.8374457903394645 9 0.26310463468933415 0.5832211754149843 >10 1.9024488969844162 8.414336274363192 >50 0.14167172637117992 2.791485967798215 >100 0.08095527221210282 3.1304521210308556 >500 0.04047763610605141 6.83415582473456 >1k 0.0 0.0 >5k 0.020238818053025704 44.374657295249484 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACCTTGCAGAATCTCGTATGCCGTC 8902 44.374657295249484 No Hit TCTTATACACATCTCCGAGCCCACGAGACCTTGCAGAATCTCGTATGCCGT 823 4.102487413389163 No Hit TTATACACATCTCCGAGCCCACGAGACCTTGCAGAATCTCGTATGCCGTCT 548 2.7316684113453964 RNA PCR Primer, Index 40 (95% over 21bp) CTCTTATACACATCTCCGAGCCCACGAGACCTTGCAGAATCTCGTATGCCG 283 1.4106973730123125 No Hit GTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCAC 124 0.618114750012462 No Hit AAAAAGTACTCTGCGTTGATACCACTGCTTCTGTCTCTTATACACATCTCC 120 0.5981755645281891 No Hit AAAAAGTACTCTGCGTTGATACCACCTGTCTCTTATACACATCTCCGAGCC 101 0.5034644334778925 No Hit GTGGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCC 97 0.4835252479936195 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 95 0.473555655251483 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 94 0.4685708588804147 No Hit AAGCAGTGGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCC 93 0.46358606250934653 No Hit AAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC 69 0.34395094960370864 No Hit CTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTC 61 0.3040725786351628 No Hit CTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGTC 51 0.25422461492448034 No Hit AAAAAAAGTACTCTGCGTTGATACCACCTGTCTCTTATACACATCTCCGAG 48 0.2392702258112756 No Hit AAAAAAAAGTACTCTGCGTTGATACCACCTGTCTCTTATACACATCTCCGA 47 0.23428542944020736 No Hit AAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGCCCA 43 0.21434624395593438 No Hit CAGTGGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAG 41 0.2043766512137979 No Hit CTTATACACATCTCCGAGCCCACGAGACGATCGTGTATCTCGTATGCCGTC 41 0.2043766512137979 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTCTGTCTCTTATACACATCTCCGA 40 0.19939185484272964 No Hit GGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCA 39 0.19440705847166143 No Hit AAAAAGTACTCTGCGTTGATACCACTGCTGTCTCTTATACACATCTCCGAG 37 0.18443746572952494 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 36 0.1794526693584567 No Hit GTGGTATCAACGCAGAGTACTTTTTTTCTGTCTCTTATACACATCTCCGAG 35 0.17446787298738844 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTCTGTCTCTTATACACATCTCCG 32 0.15951348387418374 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 31 0.1545286875031155 No Hit GTGGTATCAACGCAGAGTACTTTTTTCTGTCTCTTATACACATCTCCGAGC 28 0.13957429838991078 No Hit AAAAAGTACTCTGCGTTGATACCACTGCTTCCAAGCCTGTCTCTTATACAC 27 0.13458950201884254 No Hit AAAAAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGC 26 0.1296047056477743 No Hit CAGTGGTATCAACGCAGAGTACTTTTTTTTCTGTCTCTTATACACATCTCC 26 0.1296047056477743 No Hit AAAAAGTACTCTGCGTTGATACCACTGCTTGGAAGCCTGTCTCTTATACAC 26 0.1296047056477743 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 26 0.1296047056477743 No Hit TATACACATCTCCGAGCCCACGAGACCTTGCAGAATCTCGTATGCCGTCTT 26 0.1296047056477743 RNA PCR Primer, Index 40 (100% over 21bp) AAAAAAGTACTCTGCGTTGATACCACCTGTCTCTTATACACATCTCCGAGC 25 0.12461990927670605 No Hit AAAGTACTCTGCGTTGATACCACTGCTTCTGTCTCTTATACACATCTCCGA 24 0.1196351129056378 No Hit CAGTGGTATCAACGCAGAGTACTTTTTTTCTGTCTCTTATACACATCTCCG 24 0.1196351129056378 No Hit CTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTC 23 0.11465031653456956 No Hit CGGAGATGTGTATAAGAGACAGCTTATACACATCTCCGAGCCCACGAGACC 21 0.10468072379243307 No Hit AAGCAGTGGTATCAACGCAGAGTACTTTTTTCTGTCTCTTATACACATCTC 21 0.10468072379243307 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0 0.0 0.0 2 0.0 0.0 0.0 3 0.0 0.0 0.0 4 0.0 0.0 0.0 5 0.0 0.0 0.0 6 0.0 0.0 0.0 7 0.0 0.0 0.0 8 0.0 0.0 0.009969592742136484 9 0.0 0.0 0.04486316733961418 10 0.0 0.0 0.08474153830816011 11 0.0 0.0 0.09969592742136484 12 0.0 0.0 0.1545286875031155 13 0.0 0.0 0.17446787298738847 14 0.0 0.0 0.18443746572952494 15 0.0 0.0 0.26419420766661683 16 0.0 0.0 0.34893574597477695 17 0.0 0.0 0.43367728428293706 18 0.0 0.0 0.5333732117043019 19 0.0 0.0 0.6131299536413938 20 0.0 0.0 0.7028562883206221 21 0.0 0.0 0.8474153830816011 22 0.0 0.0 1.111609590748218 23 0.0 0.0 2.2032799960121627 24 0.0 0.0 2.906136284332785 25 0.0 0.0 3.220178455710084 26 0.0 0.0 4.541149494043168 27 0.0 0.0 5.253975375105927 28 0.0 0.0 6.430387318678032 29 0.0 0.0 7.62673844773441 30 0.0 0.0 8.489108219929216 31 0.0 0.0 10.2337869498031 32 0.0 0.0 11.036339165545087 33 0.0 0.0 11.599621155475798 34 0.0 0.0 12.721200338966153 35 0.0 0.0 13.4988285728528 36 0.0 0.0 14.435970290613628 37 0.0 0.0 15.687154179751758 38 0.0 0.0 16.52459997009122 39 0.0 0.0 17.511589651562733 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGAGACT 20 6.868575E-4 45.0 23 TGCCGTC 995 0.0 41.15578 45 GCCGTCT 85 0.0 39.705883 45 ATGCCGT 1040 0.0 39.375004 44 ATCTCGT 1055 0.0 38.815166 37 AATCTCG 1045 0.0 38.75598 36 GAATCTC 1045 0.0 38.75598 35 AGAATCT 1025 0.0 38.634144 34 CAGAATC 1025 0.0 38.634144 33 TATGCCG 1060 0.0 38.632076 43 TCTCGTA 1060 0.0 38.632076 38 CTCGTAT 1060 0.0 38.632076 39 GTATGCC 1065 0.0 38.450706 42 TCGTATG 1065 0.0 38.450706 40 CGTATGC 1070 0.0 38.271027 41 TTGCAGA 1035 0.0 38.26087 30 GCAGAAT 1035 0.0 38.26087 32 CTTGCAG 1035 0.0 38.26087 29 TGCAGAA 1035 0.0 38.26087 31 ACCTTGC 1045 0.0 38.110046 27 >>END_MODULE