H23NVBCXX l02n01 36h_31.34100000005d8b.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	H23NVBCXX l02n01 36h_31.34100000005d8b.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	20061
Sequences flagged as poor quality	0
Sequence length	51
%GC	46

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACCTTGCAGAATCTCGTATGCCGTC	8902	44.374657295249484	No Hit
TCTTATACACATCTCCGAGCCCACGAGACCTTGCAGAATCTCGTATGCCGT	823	4.102487413389163	No Hit
TTATACACATCTCCGAGCCCACGAGACCTTGCAGAATCTCGTATGCCGTCT	548	2.7316684113453964	RNA PCR Primer, Index 40 (95% over 21bp)
CTCTTATACACATCTCCGAGCCCACGAGACCTTGCAGAATCTCGTATGCCG	283	1.4106973730123125	No Hit
GTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCAC	124	0.618114750012462	No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCTGTCTCTTATACACATCTCC	120	0.5981755645281891	No Hit
AAAAAGTACTCTGCGTTGATACCACCTGTCTCTTATACACATCTCCGAGCC	101	0.5034644334778925	No Hit
GTGGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCC	97	0.4835252479936195	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	95	0.473555655251483	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	94	0.4685708588804147	No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCC	93	0.46358606250934653	No Hit
AAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC	69	0.34395094960370864	No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTC	61	0.3040725786351628	No Hit
CTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGTC	51	0.25422461492448034	No Hit
AAAAAAAGTACTCTGCGTTGATACCACCTGTCTCTTATACACATCTCCGAG	48	0.2392702258112756	No Hit
AAAAAAAAGTACTCTGCGTTGATACCACCTGTCTCTTATACACATCTCCGA	47	0.23428542944020736	No Hit
AAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGCCCA	43	0.21434624395593438	No Hit
CAGTGGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAG	41	0.2043766512137979	No Hit
CTTATACACATCTCCGAGCCCACGAGACGATCGTGTATCTCGTATGCCGTC	41	0.2043766512137979	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTCTGTCTCTTATACACATCTCCGA	40	0.19939185484272964	No Hit
GGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCA	39	0.19440705847166143	No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTGTCTCTTATACACATCTCCGAG	37	0.18443746572952494	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	36	0.1794526693584567	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTCTGTCTCTTATACACATCTCCGAG	35	0.17446787298738844	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTCTGTCTCTTATACACATCTCCG	32	0.15951348387418374	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	31	0.1545286875031155	No Hit
GTGGTATCAACGCAGAGTACTTTTTTCTGTCTCTTATACACATCTCCGAGC	28	0.13957429838991078	No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCCAAGCCTGTCTCTTATACAC	27	0.13458950201884254	No Hit
AAAAAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGC	26	0.1296047056477743	No Hit
CAGTGGTATCAACGCAGAGTACTTTTTTTTCTGTCTCTTATACACATCTCC	26	0.1296047056477743	No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTGGAAGCCTGTCTCTTATACAC	26	0.1296047056477743	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	26	0.1296047056477743	No Hit
TATACACATCTCCGAGCCCACGAGACCTTGCAGAATCTCGTATGCCGTCTT	26	0.1296047056477743	RNA PCR Primer, Index 40 (100% over 21bp)
AAAAAAGTACTCTGCGTTGATACCACCTGTCTCTTATACACATCTCCGAGC	25	0.12461990927670605	No Hit
AAAGTACTCTGCGTTGATACCACTGCTTCTGTCTCTTATACACATCTCCGA	24	0.1196351129056378	No Hit
CAGTGGTATCAACGCAGAGTACTTTTTTTCTGTCTCTTATACACATCTCCG	24	0.1196351129056378	No Hit
CTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTC	23	0.11465031653456956	No Hit
CGGAGATGTGTATAAGAGACAGCTTATACACATCTCCGAGCCCACGAGACC	21	0.10468072379243307	No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTCTGTCTCTTATACACATCTC	21	0.10468072379243307	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAGACT	20	6.868575E-4	45.0	23
TGCCGTC	995	0.0	41.15578	45
GCCGTCT	85	0.0	39.705883	45
ATGCCGT	1040	0.0	39.375004	44
ATCTCGT	1055	0.0	38.815166	37
AATCTCG	1045	0.0	38.75598	36
GAATCTC	1045	0.0	38.75598	35
AGAATCT	1025	0.0	38.634144	34
CAGAATC	1025	0.0	38.634144	33
TATGCCG	1060	0.0	38.632076	43
TCTCGTA	1060	0.0	38.632076	38
CTCGTAT	1060	0.0	38.632076	39
GTATGCC	1065	0.0	38.450706	42
TCGTATG	1065	0.0	38.450706	40
CGTATGC	1070	0.0	38.271027	41
TTGCAGA	1035	0.0	38.26087	30
GCAGAAT	1035	0.0	38.26087	32
CTTGCAG	1035	0.0	38.26087	29
TGCAGAA	1035	0.0	38.26087	31
ACCTTGC	1045	0.0	38.110046	27

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content