FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 36h_30.34100000005d7e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 36h_30.34100000005d7e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1372
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGTC312.259475218658892No Hit
CTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTC261.8950437317784257No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTC161.1661807580174928No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGTC80.5830903790087464No Hit
CTTATACACATCTCCGAGCCCACGAGACATAGACAAATCTCGTATGCCGTC80.5830903790087464No Hit
CTTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTC80.5830903790087464TruSeq Adapter, Index 12 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTC60.43731778425655976No Hit
CTTATACACATCTCCGAGCCCACGAGACGATCGTGTATCTCGTATGCCGTC50.36443148688046645No Hit
CTTATACACATCTCCGAGCCCACGAGACCTTGCAGAATCTCGTATGCCGTC40.2915451895043732No Hit
CTTATACACATCTCCGAGCCCACGAGACAGTGTCTTATCTCGTATGCCGTC30.21865889212827988No Hit
CTCACAACCATCCGTAACAAGATCTGACTCCCTCTTCTGGAGCTGTCTCTT20.1457725947521866No Hit
CTTATACACATCTCCGAGCCCACGAGACTGATTACAATCTCGTATGCCGTC20.1457725947521866No Hit
CTTATACACATCTCCGAGCCACGAGACCATTCCGAATCTCGTATGCCGTCT20.1457725947521866RNA PCR Primer, Index 18 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACTCAACTGTATCTCGTATGCCGTC20.1457725947521866No Hit
CTTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCGTC20.1457725947521866RNA PCR Primer, Index 46 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCCCAC250.001447556536.00000417
GTATGCC250.001447556536.00000442
ATCTCGT250.001447556536.00000437
GCCCACG250.001447556536.00000418
TGCCGTC250.001447556536.00000445
CCACGAG250.001447556536.00000420
TATGCCG250.001447556536.00000443
CACGAGA250.001447556536.00000421
CCCACGA250.001447556536.00000419
TCTCGTA250.001447556536.00000438
ACGAGAC250.001447556536.00000422
TATCTCG250.001447556536.00000436
ATGCCGT250.001447556536.00000444
CGTATGC250.001447556536.00000441
TCGTATG250.001447556536.00000440
CTCGTAT250.001447556536.00000439
GAGCCCA300.003547963229.99999816
TTATACA300.003547963229.9999982
CGAGCCC300.003547963229.99999815
CCGAGCC300.003547963229.99999814
Produced by FastQC (version 0.11.2)