FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 36h_3.34100000005b9a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 36h_3.34100000005b9a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1051
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGTC141.3320647002854424No Hit
CTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTC141.3320647002854424No Hit
CTTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTC111.0466222645099905TruSeq Adapter, Index 12 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACGTCTTAGTATCTCGTATGCCGTC100.9514747859181731No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTC90.8563273073263558No Hit
CTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTC70.6660323501427212No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGTC60.570884871550904No Hit
CTTATACACATCTCCGAGCCCACGAGACGATCGTGTATCTCGTATGCCGTC50.47573739295908657No Hit
TTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTCT40.3805899143672693Illumina PCR Primer Index 11 (95% over 21bp)
GCGTTGATACCACTGCTTCGCAAAAAAAAAAAAGTACTCTGCGTTGATACC30.285442435775452No Hit
ACTCTGCGTTGATACCACTGCTTGCGCAAAAAAAAAAAGTACTCTGCGTTG30.285442435775452No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.19029495718363465No Hit
CTTATACACATCTCCGAGCCCACGAGACATAGACAAATCTCGTATGCCGTC20.19029495718363465No Hit
CCACTGTACCGTATGCGAATCTTTGCACCCAACCATGTGGCTGTCTCTTAT20.19029495718363465No Hit
TTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGTCT20.19029495718363465No Hit
AAAGTACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAA20.19029495718363465No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAACTGTCTCTTATAC20.19029495718363465No Hit
CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20.19029495718363465No Hit

[FAIL]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers