FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 36h_29.34100000005d61.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 36h_29.34100000005d61.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences301751
Sequences flagged as poor quality0
Sequence length51
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTTGCAGAATCTCGTATGCCGTC50371.669257102710513No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGAA20590.6823506798651868No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCAT9360.31018952712667064No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9120.30223594950803806No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGCC8650.28666019333821596No Hit
GAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGAAACATCCACTTGA7040.23330494347988903No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5700.1888974684425238No Hit
TTATACACATCTCCGAGCCCACGAGACCTTGCAGAATCTCGTATGCCGTCT5630.18657767497042263RNA PCR Primer, Index 40 (95% over 21bp)
GTCCTACAGTGTGCATTTCTCATTTTTCACGTTTTTCAGTGATTTCGTCAT5270.17464730854247376No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5040.1670251299912842No Hit
GTCCTTCAGTGTGCATTTCTCATTTTTCACGTTTTTTAGTGATTTCGTCAT5000.1656995337215121No Hit
CTGTAGGACATGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGAA4800.15907155237265164No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTAG4590.1521121719563481No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4360.14448999340515856No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4110.13620501671908297No Hit
ACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGAAACATCCA3670.12162345775158989No Hit
ATTCCAGGTCCTTCAGTGTGCATTTCTCATTTTTCACGTTTTTTAGTGATT3660.12129205868414686No Hit
CCATATTTCACGTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGT3390.11234428386318521No Hit
GCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3250.10770469691898288No Hit
TTCCAGGTCCTTCAGTGTGCATTTCTCATTTTTCACGTTTTTTAGTGATTT3180.1053849034468817No Hit
GTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGAAAC3020.10008251836779332No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGAGA6400.040.07812521
TATGCCG6350.040.0393743
CGAGACC6250.039.96000323
CCACGAG6450.039.7674420
ATGCCGT6400.039.72656244
GCCCACG6500.039.4615418
CCCACGA6500.039.4615419
TCGTATG6450.039.41860240
ACGAGAC6450.039.41860222
TCTCGTA6400.039.37538
CTTGCAG6400.039.37529
CGTATGC6500.039.11538741
ATCTCGT6450.039.06976737
TGCCGTC6550.038.81679545
CTCGTAT6500.038.7692339
AATCTCG6450.038.7209336
TTGCAGA6500.038.42307730
ACCTTGC6500.038.42307727
GAGACCT6550.038.12977224
CGAGCCC6750.038.015
Produced by FastQC (version 0.11.2)