##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename H23NVBCXX l02n01 36h_28.34100000005d55.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 9385 Sequences flagged as poor quality 0 Sequence length 51 %GC 51 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 35.83761321257325 38.0 32.0 38.0 32.0 38.0 2 36.182631859350025 38.0 38.0 38.0 32.0 38.0 3 36.02450719232818 38.0 32.0 38.0 32.0 38.0 4 36.32882258923814 38.0 38.0 38.0 32.0 38.0 5 36.30793819925413 38.0 38.0 38.0 32.0 38.0 6 38.52456046883324 40.0 38.0 40.0 38.0 40.0 7 37.949280767181676 40.0 38.0 40.0 32.0 40.0 8 37.90037293553543 40.0 38.0 40.0 32.0 40.0 9 38.21523708044752 40.0 38.0 40.0 38.0 40.0 10 38.00681939264784 40.0 38.0 40.0 32.0 40.0 11 38.22589238145978 40.0 38.0 40.0 38.0 40.0 12 37.685562067128394 38.0 38.0 40.0 32.0 40.0 13 38.17282898241875 40.0 38.0 40.0 38.0 40.0 14 37.949280767181676 40.0 38.0 40.0 32.0 40.0 15 37.826851358550876 40.0 38.0 40.0 32.0 40.0 16 37.27703782631859 40.0 38.0 40.0 32.0 40.0 17 37.165370271710174 40.0 38.0 40.0 32.0 40.0 18 36.92679808204582 38.0 38.0 40.0 32.0 40.0 19 37.29653702717102 40.0 38.0 40.0 32.0 40.0 20 37.5470431539691 40.0 38.0 40.0 32.0 40.0 21 37.661267980820455 40.0 38.0 40.0 32.0 40.0 22 37.802237613212576 40.0 38.0 40.0 32.0 40.0 23 37.74629728289824 40.0 38.0 40.0 32.0 40.0 24 37.50079914757592 40.0 38.0 40.0 32.0 40.0 25 37.05125199786894 40.0 38.0 40.0 32.0 40.0 26 37.0141715503463 38.0 38.0 40.0 32.0 40.0 27 37.21523708044752 40.0 38.0 40.0 32.0 40.0 28 37.405966968566865 40.0 38.0 40.0 32.0 40.0 29 37.57932871603623 40.0 38.0 40.0 32.0 40.0 30 37.60490143846564 38.0 38.0 40.0 32.0 40.0 31 37.75652637187 40.0 38.0 40.0 32.0 40.0 32 37.40298348428343 38.0 38.0 40.0 32.0 40.0 33 37.45796483750666 40.0 38.0 40.0 32.0 40.0 34 37.59563132658498 40.0 38.0 40.0 32.0 40.0 35 37.48993074054342 40.0 38.0 40.0 32.0 40.0 36 37.00703249866809 38.0 38.0 40.0 32.0 40.0 37 37.157059136920616 38.0 38.0 40.0 32.0 40.0 38 37.356526371870004 38.0 38.0 40.0 32.0 40.0 39 36.875546084176875 38.0 38.0 40.0 32.0 40.0 40 36.89216835375599 38.0 38.0 40.0 32.0 40.0 41 37.211614278103355 38.0 38.0 40.0 32.0 40.0 42 37.15386254661694 38.0 38.0 40.0 32.0 40.0 43 37.76089504528503 40.0 38.0 40.0 32.0 40.0 44 37.27948854555141 40.0 38.0 40.0 32.0 40.0 45 37.525306339904105 40.0 38.0 40.0 32.0 40.0 46 36.89025039957379 38.0 38.0 40.0 32.0 40.0 47 36.78923814597762 38.0 38.0 40.0 32.0 40.0 48 36.911241342567926 38.0 38.0 40.0 32.0 40.0 49 36.55812466702184 38.0 38.0 40.0 32.0 40.0 50 36.279595098561536 38.0 38.0 40.0 27.0 40.0 51 36.07341502397443 38.0 38.0 40.0 27.0 40.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 2201 1 0.7387220213754944 2201 2 0.9056849338178097 2201 3 0.5750701248651211 2201 4 -0.7459285014843289 2201 5 0.8126867978109971 2201 6 1.0646626774444812 2201 7 1.3479401351196572 2201 8 -0.6296216528166809 2201 9 0.6831933367119873 2201 10 0.853902304360048 2201 11 1.4048876948644775 2201 12 1.6066397780818136 2201 13 1.3225391888329696 2201 14 0.6955023621002994 2201 15 0.7445615342716394 2201 16 -0.8516881259945492 2201 17 -1.9120648191704461 2201 18 -2.630245878025484 2201 19 1.147900865179075 2201 20 1.4303281801080843 2201 21 -0.7664656532757093 2201 22 0.9025910613831485 2201 23 1.1618630573261157 2201 24 0.6360168851884609 2201 25 1.569116468300166 2201 26 1.0648844320749689 2201 27 1.7051496342118995 2201 28 1.520516693059271 2201 29 1.6704430244677866 2201 30 1.1075460405338973 2201 31 -0.12137161507383354 2201 32 1.658189666129168 2201 33 2.1238528638302583 2201 34 1.965484754699645 2201 35 1.1729935600309815 2201 36 1.9540345983753724 2201 37 1.0205529897250543 2201 38 1.4213735378559704 2201 39 1.6407290541469663 2201 40 2.529267842983245 2201 41 1.64845008013787 2201 42 -0.1514686676681123 2201 43 1.4502680735640823 2201 44 -0.5504566246124867 2201 45 0.9763833953311405 2201 46 1.2006414654102358 2201 47 2.0127160503164205 2201 48 0.315253097610281 2201 49 2.433890879139888 2201 50 1.9939698612468462 2201 51 1.4742897904469245 1101 1 -1.9920472093937391 1101 2 -0.4020073738744969 1101 3 -1.1556991059041124 1101 4 -0.4766977322535624 1101 5 -1.4950055098813095 1101 6 0.2697908825726856 1101 7 -1.0494957623162406 1101 8 0.4280706548756257 1101 9 0.32421897773762964 1101 10 -1.671738721280981 1101 11 0.3664261564029374 1101 12 0.5938192652613026 1101 13 0.4379238042175828 1101 14 0.6570408236387593 1101 15 -1.8451820554719447 1101 16 0.5842093099028816 1101 17 -4.00180840891403 1101 18 -1.732809980589586 1101 19 -0.9418427245645091 1101 20 0.13545638523628867 1101 21 -0.6703118071218626 1101 22 -3.840998682206596 1101 23 -2.3637779683149134 1101 24 -3.5178292686576924 1101 25 0.22296262214631923 1101 26 0.12898699617753806 1101 27 1.3461752752375418 1101 28 0.23846541100799357 1101 29 0.8114686654934289 1101 30 -0.020659087671226928 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0.43036315605672826 2202 17 2.3058838987782764 2202 18 1.9594977117181003 2202 19 1.9043111215893305 2202 20 1.5200717698516684 2202 21 1.8681497313396775 2202 22 1.4666936254857177 2202 23 1.7131451086081668 2202 24 1.0975553467269208 2202 25 -0.39242199323829396 2202 26 0.7443716115621513 2202 27 1.0384829675452352 2202 28 1.3153884879310667 2202 29 -1.111608257583491 2202 30 0.44087937386723297 2202 31 -1.2944485381507533 2202 32 -2.0982205902810875 2202 33 -0.8376855977082016 2202 34 0.01676680598169611 2202 35 1.6088909959284123 2202 36 -0.7895551452143721 2202 37 -0.9281649589928946 2202 38 -0.8606777441953071 2202 39 0.15354956696747735 2202 40 0.6574729711883691 2202 41 -0.1848832531954656 2202 42 0.4703262041267635 2202 43 0.5912937145897246 2202 44 1.4815946574387908 2202 45 -0.8313089123611661 2202 46 -1.4147431499743774 2202 47 2.281946819547187 2202 48 1.8473043796615585 2202 49 -0.6173911721421632 2202 50 0.7375596048365907 2202 51 1.5960846622418003 1102 1 0.9530077356612097 1102 2 0.619970648103525 1102 3 -0.5677870179920248 1102 4 0.11121435565852522 1102 5 0.955543940668143 1102 6 0.5884722012539996 1102 7 1.1812734684529929 1102 8 0.6918069186118885 1102 9 1.159383812902469 1102 10 0.7348546853124276 1102 11 0.3334591234359081 1102 12 0.2971159685580034 1102 13 0.6796820459758237 1102 14 0.7669309335288688 1102 15 1.077894867604975 1102 16 1.957835683529261 1102 17 1.6355542284486049 1102 18 0.7507065029268958 1102 19 1.695519912798126 1102 20 1.4541377039176027 1102 21 0.6263914895814366 1102 22 0.9502101090021995 1102 23 1.471386866849926 1102 24 1.493159742331315 1102 25 0.28340218258588123 1102 26 1.0410749082654505 1102 27 0.10991153897380457 1102 28 1.2824214549640374 1102 29 1.5037763578011223 1102 30 -0.20197776898991293 1102 31 -0.44280018650240294 1102 32 1.4677134756529782 1102 33 0.8381385781159736 1102 34 0.1321514213663093 1102 35 1.6968030838405 1102 36 0.7873679317087081 1102 37 1.0443625135345727 1102 38 1.4689925854750214 1102 39 2.4502528636707765 1102 40 2.7673630810784786 1102 41 1.6722596039473885 1102 42 2.217578951379508 1102 43 1.5693156926117027 1102 44 1.580495756339893 1102 45 2.04781196675971 1102 46 1.6292128939816664 1102 47 -0.5587125211121489 1102 48 0.8747769071340912 1102 49 1.1957956410446542 1102 50 -0.8393634720864895 1102 51 -0.29951973336259385 2203 1 0.7053886880421629 2203 2 -0.5609817328488589 2203 3 0.9417367915317882 2203 4 -0.31259516815099886 2203 5 1.4126867978109985 2203 6 -0.6686706558888531 2203 7 0.24794013511966284 2203 8 -0.3462883194833495 2203 9 0.2165266700453259 2203 10 -1.5794310289732891 2203 11 -0.028445638468852508 2203 12 1.0399731114151507 2203 13 0.2892058554996382 2203 14 -0.2711643045663692 2203 15 1.277894867604978 2203 16 1.881645207338785 2203 17 0.7212685141628867 2203 18 -0.16357921135882236 2203 19 0.2479008651790764 2203 20 0.9303281801080843 2203 21 0.6168676800576236 2203 22 0.43592439471648703 2203 23 0.6618630573261157 2203 24 -0.4306497814782091 2203 25 -0.5308835316998355 2203 26 0.29821776540830314 2203 27 0.43848296754523375 2203 28 0.12051669305927248 2203 29 0.7037763578011251 2203 30 1.7742127072005687 2203 31 1.2952950515928308 2203 32 1.4915229994624966 2203 33 -0.5761471361697446 2203 34 1.5654847546996393 2203 35 2.0396602266976487 2203 36 -0.9792987349579576 2203 37 0.02055298972505426 2203 38 -0.5786264621440296 2203 39 0.5740623874802964 2203 40 -0.4040654903500922 2203 41 1.3484500801378658 2203 42 0.5318646656652248 2203 43 0.5502680735640837 2203 44 1.466210042054179 2203 45 1.076383395331142 2203 46 2.000641465410233 2203 47 0.5127160503164205 2203 48 0.36525309761027813 2203 49 0.5672242124732207 2203 50 2.127303194580179 2203 51 1.4576231237802588 1103 1 0.39256817522164766 1103 2 -0.940468912336037 1103 3 -0.1556991059041124 1103 4 -2.0151592707150954 1103 5 -0.03346704834284964 1103 6 -1.1148245020427012 1103 7 -0.5879573007777736 1103 8 -2.1873139605089875 1103 9 -1.2911656376469836 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47 -0.17478394968357946 1214 48 -1.5180802357230547 1214 49 -1.5452757875267764 1214 50 -0.46436347208648954 1214 51 0.057623123780260244 1215 1 -1.6436309198009766 1215 2 0.08215552205310672 1215 3 0.36918777192394714 1215 4 0.3423067926333161 1215 5 0.40092209192864203 1215 6 0.9077999323464425 1215 7 -0.5246088844881811 1215 8 0.47331952365390606 1215 9 0.840056081810026 1215 10 0.02056897102671229 1215 11 -1.2715828933708195 1215 12 -1.5012033591730827 1215 13 0.35195095353885364 1215 14 -0.8633211673114687 1215 15 0.019071338193207055 1215 16 0.40321383478976003 1215 17 -0.8434373681900524 1215 18 -0.08122627018234851 1215 19 0.7459400808653527 1215 20 1.1264066114806326 1215 21 -0.5752891826874773 1215 22 0.6476891005988392 1215 23 1.152059135757483 1215 24 -0.24633605598801012 1215 25 1.186763527123695 1215 26 -0.3958998816505215 1215 27 -0.37328173833711986 1215 28 -1.2049735030191613 1215 29 -0.20210599514005168 1215 30 -0.9708853320151221 1215 31 -0.018430438603246557 1215 32 0.17779750926641924 1215 33 1.3297352167714322 1215 34 -0.3678485786336907 1215 35 1.0749543443447038 1215 36 2.22854440229694 1215 37 -0.31278034360828144 1215 38 -0.7158813641048098 1215 39 -1.6926042791863694 1215 40 -0.4511243138795038 1215 41 -0.47900090025429165 1215 42 0.34362937154757844 1215 43 -1.245810357808466 1215 44 -0.07496642853405433 1215 45 -0.5236166046688595 1215 46 -3.4464173581191773 1215 47 1.1009513444340655 1215 48 -1.1210214121936417 1215 49 0.8848712712967526 1215 50 0.7782835867370395 1215 51 -1.2364945232785658 1212 1 -0.33270655005307503 1212 2 1.477113505246379 1212 3 0.7893558391508364 1212 4 0.896928641372817 1212 5 0.5269725120967124 1212 6 0.5884722012539996 1212 7 1.3241306113101388 1212 8 1.4060926328976038 1212 9 1.4450980986167536 1212 10 1.1634261138838582 1212 11 0.9048876948644775 1212 12 1.8685445399865799 1212 13 1.1082534745472543 1212 14 1.1955023621002994 1212 15 1.220752010462121 1212 16 1.5292642549578304 1212 17 1.492697085591459 1212 18 1.1792779314983264 1212 19 0.7669484842266954 1212 20 -0.18871943893953613 1212 21 0.41210577529572134 1212 22 0.7359243947164842 1212 23 -0.17147027600721998 1212 24 1.3503025994741762 1212 25 1.7834021825858812 1212 26 2.1839320511225893 1212 27 2.038482967545235 1212 28 1.8538500263926068 1212 29 1.2180620720868376 1212 30 1.298022231010087 1212 31 1.4857712420690206 1212 32 1.6819991899386864 1212 33 1.4809957209731124 1212 34 1.06072284993774 1212 35 1.8396602266976458 1212 36 2.287367931708708 1212 37 2.258648227820288 1212 38 0.11184972833216023 1212 39 0.8073957208136306 1212 40 -0.6612083474929449 1212 41 1.0294024610902497 1212 42 1.7890075228080775 1212 43 -0.35925573595972793 1212 44 0.6519243277684623 1212 45 -1.095045176097429 1212 46 2.4863557511245205 1212 47 1.7984303360307052 1212 48 2.0890626214197994 1212 49 0.2672242124732236 1212 50 2.6606365279135105 1212 51 1.343337409494545 1213 1 0.17989849196372631 1213 2 1.1998025808766357 1213 3 0.36918777192394714 1213 4 0.6952479691039031 1213 5 -1.069666143365474 1213 6 1.0254469911699715 1213 7 0.8871558213941668 1213 8 0.47331952365390606 1213 9 -1.336414506425264 1213 10 0.6088042651443573 1213 11 0.9637112242762456 1213 12 -0.20708571211426374 1213 13 -9.902229317333422E-4 1213 14 0.6072670679826544 1213 15 0.37201251466380114 1213 16 0.520860893613289 1213 17 -1.6081432505429945 1213 18 0.21289137687647752 1213 19 -1.3717069779581763 1213 20 -0.3441816238134834 1213 21 0.6600049349595878 1213 22 1.2947479241282522 1213 23 1.152059135757483 1213 24 1.5771933557766928 1213 25 -0.6955894140527761 1213 26 0.5452765889377176 1213 27 0.27377708519229316 1213 28 -0.5579146794897483 1213 29 -0.7903412892577037 1213 30 0.08793819739664599 1213 31 -1.2537245562503045 1213 32 1.1777975092664192 1213 33 -1.3761471361697417 1213 34 0.6909749507780774 1213 35 1.1926014031682328 1213 36 -1.6538085388795238 1213 37 0.5107490681564215 1213 38 0.6958833417775381 1213 39 -1.1631925144804853 1213 40 -0.4511243138795038 1213 41 -0.3025303120189946 1213 42 -1.0681353343347766 1213 43 0.695366112779773 1213 44 -0.7220252520634673 1213 45 1.0646186894487855 1213 46 0.7888767595278807 1213 47 2.2185984032575945 1213 48 0.23191976427694527 1213 49 0.6495771536496946 1213 50 0.9547541749723365 1213 51 0.057623123780260244 >>END_MODULE >>Per sequence quality scores pass #Quality Count 17 1.0 18 0.0 19 0.0 20 0.0 21 0.0 22 5.0 23 7.0 24 14.0 25 26.0 26 47.0 27 81.0 28 103.0 29 136.0 30 184.0 31 230.0 32 250.0 33 334.0 34 414.0 35 490.0 36 603.0 37 847.0 38 1540.0 39 4073.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 5.733162830349531 3.250213128729753 7.534100596760443 83.48252344416028 2 3.0477408354646207 3.0690537084398977 88.88533674339301 4.997868712702473 3 3.612147043153969 8.843899840170485 83.2605221097496 4.283431006925946 4 2.2802344166222697 81.80074587107086 11.411827384123601 4.507192328183271 5 2.9621736814065 11.635588705380927 82.95151838039425 2.450719232818327 6 4.198188598827917 84.7735748534896 4.123601491742142 6.9046350559403304 7 4.006393180607352 11.124134256792754 4.230154501864678 80.63931806073522 8 4.070324986680874 83.88918486947257 3.612147043153969 8.428343100692596 9 4.091635588705381 8.311134789557805 3.953116675546084 83.64411294619073 10 3.345764517847629 82.29088971763451 9.706979222163026 4.656366542354822 11 4.44326052210975 5.434203516249334 82.28023441662226 7.842301545018647 12 3.3670751198721365 5.093233883857219 10.346297282898242 81.1933937133724 13 4.283431006925946 3.431006925945658 82.7810335641982 9.504528502930208 14 3.399041022908897 4.613745338305807 5.221097496004262 86.76611614278103 15 8.396377197655834 5.242408098028769 4.091635588705381 82.26957911561001 16 82.12040490143848 8.939797549280767 3.601491742141715 5.338305807139052 17 9.206180074587108 83.06872669152904 3.718700053276505 4.006393180607352 18 82.6851358550879 5.082578582844965 4.240809802876931 7.991475759190197 19 4.730953649440597 5.1891315929675015 4.0170484816196055 86.0628662759723 20 4.112946190729888 4.070324986680874 4.006393180607352 87.81033564198188 21 3.612147043153969 9.525839104954716 3.7506659563132656 83.11134789557805 22 4.123601491742142 82.15237080447523 3.740010655301012 9.984017048481618 23 7.863612147043154 5.700586041555674 4.240809802876931 82.19499200852424 24 81.11880660628663 9.206180074587108 4.635055940330314 5.039957378795951 25 9.504528502930208 81.85402237613214 4.890783164624401 3.7506659563132656 26 81.23601491742141 10.527437400106553 4.762919552477358 3.473628129994672 27 4.3686734150239745 82.42940863079382 4.85881726158764 8.343100692594566 28 3.6334576451784764 6.265316995205114 4.496537027171017 85.60468833244539 29 3.558870538092701 5.27437400106553 9.025039957378796 82.14171550346298 30 3.516249334043687 5.12519978689398 85.37027171017581 5.988279168886521 31 7.7996803409696325 4.603090037293554 82.51465103889186 5.082578582844965 32 80.51145444858817 5.348961108151305 5.764517847629196 8.375066595631326 33 4.656366542354822 8.716036228023441 5.647309536494406 80.98028769312732 34 8.524240809802878 80.86307938199255 4.485881726158764 6.126798082045818 35 80.04262120404901 10.239744272775706 5.327650506126798 4.389984017048482 36 4.805540756526372 85.45551411827384 5.082578582844965 4.656366542354822 37 3.3883857218966438 83.30314331379861 9.344698987746405 3.9637719765583377 38 3.835908364411295 5.551411827384124 81.79009057005861 8.822589238145978 39 3.399041022908897 4.826851358550879 10.186467767714438 81.5876398508258 40 4.272775705913692 3.5801811401172086 82.31220031965904 9.834842834310068 41 7.810335641981886 3.7932871603622806 6.0841768779968035 82.31220031965904 42 80.58604155567394 3.7506659563132656 9.802876931273309 5.860415556739478 43 4.166222695791156 8.407032498668087 82.226957911561 5.199786893979755 44 3.4842834310069257 81.32125732551944 10.484816196057539 4.7096430474160895 45 7.128396377197656 5.466169419286095 82.72775705913692 4.677677144379329 46 81.02290889717635 4.294086307938199 5.65796483750666 9.025039957378796 47 4.187533297815663 4.421949920085242 5.508790623335109 85.88172615876398 48 8.375066595631326 3.8998401704848162 4.539158231220032 83.18593500266383 49 80.05327650506126 4.85881726158764 9.23814597762387 5.849760255727224 50 4.262120404901439 3.931806073521577 82.23761321257325 9.56846030900373 51 2.898241875332978 3.9637719765583377 10.506126798082045 82.63185935002664 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.5 16 1.0 17 0.5 18 0.0 19 1.0 20 2.0 21 1.5 22 1.0 23 0.5 24 0.0 25 1.0 26 2.0 27 2.0 28 7.0 29 12.0 30 16.0 31 20.0 32 46.0 33 72.0 34 90.0 35 108.0 36 103.5 37 99.0 38 89.0 39 79.0 40 86.5 41 94.0 42 97.0 43 100.0 44 97.5 45 95.0 46 112.5 47 130.0 48 174.0 49 218.0 50 523.5 51 829.0 52 4031.5 53 7234.0 54 3689.5 55 145.0 56 98.0 57 51.0 58 41.0 59 31.0 60 26.5 61 22.0 62 16.0 63 10.0 64 10.0 65 10.0 66 7.0 67 4.0 68 5.0 69 6.0 70 4.0 71 2.0 72 1.5 73 1.0 74 1.0 75 1.0 76 1.0 77 1.0 78 1.0 79 1.0 80 1.0 81 1.0 82 1.0 83 0.5 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.010655301012253596 2 0.010655301012253596 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 9385.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 15.045285029302077 #Duplication Level Percentage of deduplicated Percentage of total 1 89.1643059490085 13.415023974427278 2 4.603399433427762 1.3851891315929674 3 1.9830028328611897 0.8950452850293021 4 1.0623229461756374 0.6393180607352157 5 0.9915014164305949 0.7458710708577517 6 0.49575070821529743 0.44752264251465107 7 0.3541076487252125 0.37293553542887586 8 0.2124645892351275 0.2557272242940863 9 0.0708215297450425 0.09589770911028236 >10 0.84985835694051 2.237613212573255 >50 0.0 0.0 >100 0.141643059490085 5.285029302077784 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0708215297450425 74.22482685135854 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACCTTGCAGAATCTCGTATGCCGTC 6966 74.22482685135854 No Hit TTATACACATCTCCGAGCCCACGAGACCTTGCAGAATCTCGTATGCCGTCT 394 4.198188598827917 RNA PCR Primer, Index 40 (95% over 21bp) TCTTATACACATCTCCGAGCCCACGAGACCTTGCAGAATCTCGTATGCCGT 102 1.0868407032498668 No Hit CGGAGATGTGTATAAGAGACAGCTTATACACATCTCCGAGCCCACGAGACC 46 0.49014384656366544 No Hit CTCTTATACACATCTCCGAGCCCACGAGACCTTGCAGAATCTCGTATGCCG 36 0.38359083644112946 No Hit ATACACATCTCCGAGCCCACGAGACCTTGCAGAATCTCGTATGCCGTCTTC 18 0.19179541822056473 RNA PCR Primer, Index 40 (100% over 22bp) GGAGATGTGTATAAGAGACAGCTTATACACATCTCCGAGCCCACGAGACCT 16 0.17048481619605754 No Hit CTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGTC 13 0.13851891315929674 No Hit CTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTC 13 0.13851891315929674 No Hit CTTATACACATCTCCGAGCCCACGAGACCTTGCAGAATATCGTATGCCGTC 12 0.12786361214704314 No Hit CTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGTC 12 0.12786361214704314 No Hit TATACACATCTCCGAGCCCACGAGACCTTGCAGAATCTCGTATGCCGTCTT 12 0.12786361214704314 RNA PCR Primer, Index 40 (100% over 21bp) GCGTCAGATGTGTATAAGAGACAGCTTATACACATCTCCGAGCCCACGAGA 12 0.12786361214704314 No Hit CTTATACACATCTCCGAGCCCACGAGACCTTGCAGAATCTCGTATGCCGAC 10 0.10655301012253596 No Hit CTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTC 10 0.10655301012253596 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0 0.0 0.0 2 0.0 0.0 0.0 3 0.0 0.0 0.0 4 0.0 0.0 0.0 5 0.0 0.0 0.0 6 0.0 0.0 0.0 7 0.0 0.0 0.0 8 0.0 0.0 0.0 9 0.0 0.0 0.0 10 0.0 0.0 0.0 11 0.0 0.0 0.0 12 0.0 0.0 0.021310602024507193 13 0.0 0.0 0.031965903036760786 14 0.0 0.0 0.031965903036760786 15 0.0 0.0 0.07458710708577518 16 0.0 0.0 0.09589770911028236 17 0.0 0.0 0.15982951518380395 18 0.0 0.0 0.2983484283431007 19 0.0 0.0 0.38359083644112946 20 0.0 0.0 0.4581779435269046 21 0.0 0.0 0.4581779435269046 22 0.0 0.0 0.46883324453915826 23 0.0 0.0 0.47948854555141185 24 0.0 0.0 0.47948854555141185 25 0.0 0.0 0.47948854555141185 26 0.0 0.0 0.47948854555141185 27 0.0 0.0 0.5327650506126798 28 0.0 0.0 0.5647309536494406 29 0.0 0.0 0.5966968566862014 30 0.0 0.0 0.6393180607352158 31 0.0 0.0 0.7565263718700054 32 0.0 0.0 0.9163558870538092 33 0.0 0.0 1.1188066062866275 34 0.0 0.0 1.3851891315929674 35 0.0 0.0 1.5556739477890251 36 0.0 0.0 1.7581246670218433 37 0.0 0.0 2.0245071923281834 38 0.0 0.0 2.3335109216835375 39 0.0 0.0 2.717101758124667 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCCGTCT 40 5.657057E-9 45.0 45 TGCCGTC 785 0.0 42.70701 45 CTTATAC 810 0.0 41.944443 1 AATCTCG 800 0.0 41.625 36 GAATCTC 800 0.0 41.625 35 TCGTATG 820 0.0 41.43293 40 ATCTCGT 815 0.0 41.411045 37 TCTCGTA 815 0.0 41.411045 38 CGTATGC 815 0.0 41.411045 41 CTCGTAT 815 0.0 41.411045 39 ATGCCGT 810 0.0 41.388885 44 TTGCAGA 805 0.0 41.36646 30 AGAATCT 805 0.0 41.36646 34 GCAGAAT 805 0.0 41.36646 32 CAGAATC 805 0.0 41.36646 33 TGCAGAA 805 0.0 41.36646 31 CCTTGCA 800 0.0 41.34375 28 CTTGCAG 800 0.0 41.34375 29 GTATGCC 815 0.0 41.13497 42 TATGCCG 815 0.0 41.13497 43 >>END_MODULE