FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 36h_28.34100000005d55.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 36h_28.34100000005d55.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9385
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTTGCAGAATCTCGTATGCCGTC696674.22482685135854No Hit
TTATACACATCTCCGAGCCCACGAGACCTTGCAGAATCTCGTATGCCGTCT3944.198188598827917RNA PCR Primer, Index 40 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACCTTGCAGAATCTCGTATGCCGT1021.0868407032498668No Hit
CGGAGATGTGTATAAGAGACAGCTTATACACATCTCCGAGCCCACGAGACC460.49014384656366544No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCTTGCAGAATCTCGTATGCCG360.38359083644112946No Hit
ATACACATCTCCGAGCCCACGAGACCTTGCAGAATCTCGTATGCCGTCTTC180.19179541822056473RNA PCR Primer, Index 40 (100% over 22bp)
GGAGATGTGTATAAGAGACAGCTTATACACATCTCCGAGCCCACGAGACCT160.17048481619605754No Hit
CTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGTC130.13851891315929674No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTC130.13851891315929674No Hit
CTTATACACATCTCCGAGCCCACGAGACCTTGCAGAATATCGTATGCCGTC120.12786361214704314No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGTC120.12786361214704314No Hit
TATACACATCTCCGAGCCCACGAGACCTTGCAGAATCTCGTATGCCGTCTT120.12786361214704314RNA PCR Primer, Index 40 (100% over 21bp)
GCGTCAGATGTGTATAAGAGACAGCTTATACACATCTCCGAGCCCACGAGA120.12786361214704314No Hit
CTTATACACATCTCCGAGCCCACGAGACCTTGCAGAATCTCGTATGCCGAC100.10655301012253596No Hit
CTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTC100.10655301012253596No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTCT405.657057E-945.045
TGCCGTC7850.042.7070145
CTTATAC8100.041.9444431
AATCTCG8000.041.62536
GAATCTC8000.041.62535
TCGTATG8200.041.4329340
ATCTCGT8150.041.41104537
TCTCGTA8150.041.41104538
CGTATGC8150.041.41104541
CTCGTAT8150.041.41104539
ATGCCGT8100.041.38888544
TTGCAGA8050.041.3664630
AGAATCT8050.041.3664634
GCAGAAT8050.041.3664632
CAGAATC8050.041.3664633
TGCAGAA8050.041.3664631
CCTTGCA8000.041.3437528
CTTGCAG8000.041.3437529
GTATGCC8150.041.1349742
TATGCCG8150.041.1349743
Produced by FastQC (version 0.11.2)