FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 36h_27.34100000005d48.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 36h_27.34100000005d48.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences447077
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTTGCAGAATCTCGTATGCCGTC15650.3500515571143226No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCACGAG2200.036.81820
ACGAGAC2150.036.62772422
TATGCCG2250.035.9998243
GCCCACG2350.035.42535418
AATCTCG2100.035.35696836
CTCGTAT2250.034.99982539
TCTCGTA2250.034.99982538
CCGAGCC2400.034.6873314
CGAGCCC2400.034.6873315
CGTATGC2350.034.4679141
CACGAGA2350.034.4679121
ATGCCGT2400.033.74983244
ATCTCCG2550.033.52924710
TGCCGTC2450.033.06105845
TCGTATG2400.032.81233640
CCCACGA2550.032.64689619
CGAGACC2350.031.59558723
ATCTCGT2650.029.71683337
CTCCGAG2850.029.2103812
TCTCCGA2900.028.70675511
Produced by FastQC (version 0.11.2)