FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 36h_26.34100000005d3b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 36h_26.34100000005d3b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences307892
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTTGCAGAATCTCGTATGCCGTC16540.5372013563197484No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCTCG2300.037.17324436
TATGCCG2500.036.89933843
CCCACGA2500.036.89933819
TCTCGTA2450.036.7340338
CGAGACC2300.036.19523
ATCTCGT2500.035.9993537
CTCGTAT2500.035.9993539
CTCCGAG2500.035.9993512
ACGAGAC2500.035.9993522
GCCCACG2550.035.29348418
TCCGAGC2550.035.29348413
TCGTATG2550.035.29348440
CGAGCCC2550.035.29348415
TCTCCGA2550.035.29348411
TGCCGTC2550.035.29348445
CACGAGA2600.034.6147621
ATGCCGT2600.034.6147644
CCGAGCC2650.033.9616514
CCACGAG2650.033.9616520
GGTATCA801.8189894E-1233.776821
Produced by FastQC (version 0.11.2)