##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename H23NVBCXX l02n01 36h_25.34100000005d11.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 85672 Sequences flagged as poor quality 0 Sequence length 51 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.335593892987205 38.0 38.0 38.0 32.0 38.0 2 36.35047623494257 38.0 38.0 38.0 32.0 38.0 3 36.30987954057335 38.0 38.0 38.0 32.0 38.0 4 36.45251657484359 38.0 38.0 38.0 32.0 38.0 5 36.45471099075544 38.0 38.0 38.0 32.0 38.0 6 38.3590554673639 40.0 38.0 40.0 38.0 40.0 7 38.265967877486226 40.0 38.0 40.0 38.0 40.0 8 38.22685358110001 40.0 38.0 40.0 38.0 40.0 9 38.270426743860305 40.0 38.0 40.0 38.0 40.0 10 38.150329162386775 40.0 38.0 40.0 38.0 40.0 11 38.132610421141095 40.0 38.0 40.0 38.0 40.0 12 38.063953216920346 40.0 38.0 40.0 38.0 40.0 13 38.073454570921655 40.0 38.0 40.0 38.0 40.0 14 37.99718694555981 40.0 38.0 40.0 32.0 40.0 15 38.07243906994117 40.0 38.0 40.0 38.0 40.0 16 38.09329769352881 40.0 38.0 40.0 38.0 40.0 17 38.06859884209543 40.0 38.0 40.0 38.0 40.0 18 38.09904052665982 40.0 38.0 40.0 38.0 40.0 19 38.11594219815109 40.0 38.0 40.0 38.0 40.0 20 38.120751237277055 40.0 38.0 40.0 38.0 40.0 21 38.13069614343076 40.0 38.0 40.0 38.0 40.0 22 38.12852507236904 40.0 38.0 40.0 38.0 40.0 23 38.101083201045846 40.0 38.0 40.0 38.0 40.0 24 38.104304790363244 40.0 38.0 40.0 38.0 40.0 25 38.06974273975161 40.0 38.0 40.0 38.0 40.0 26 38.01019002708003 40.0 38.0 40.0 32.0 40.0 27 37.98216453450369 40.0 38.0 40.0 32.0 40.0 28 37.949423382201886 40.0 38.0 40.0 32.0 40.0 29 37.9347394714726 40.0 38.0 40.0 32.0 40.0 30 37.872280324960315 40.0 38.0 40.0 32.0 40.0 31 37.85962741619199 40.0 38.0 40.0 32.0 40.0 32 37.79742506303109 40.0 38.0 40.0 32.0 40.0 33 37.76628303296293 40.0 38.0 40.0 32.0 40.0 34 37.76365673732375 40.0 38.0 40.0 32.0 40.0 35 37.71667522644505 40.0 38.0 40.0 32.0 40.0 36 37.61263890185825 40.0 38.0 40.0 32.0 40.0 37 37.620319357549725 40.0 38.0 40.0 32.0 40.0 38 37.577189746941826 40.0 38.0 40.0 32.0 40.0 39 37.48078718834625 40.0 38.0 40.0 32.0 40.0 40 37.401543094593336 40.0 38.0 40.0 32.0 40.0 41 37.349670837613225 40.0 38.0 40.0 32.0 40.0 42 37.296654682976936 40.0 38.0 40.0 32.0 40.0 43 37.240148473246805 40.0 38.0 40.0 32.0 40.0 44 37.15014240358577 40.0 38.0 40.0 32.0 40.0 45 37.042452609954246 40.0 38.0 40.0 32.0 40.0 46 36.95827108039966 40.0 38.0 40.0 32.0 40.0 47 36.90592025399197 40.0 38.0 40.0 32.0 40.0 48 36.84309926230274 40.0 38.0 40.0 32.0 40.0 49 36.80082173872444 40.0 38.0 40.0 32.0 40.0 50 36.732503034830515 40.0 38.0 40.0 32.0 40.0 51 36.31461854514894 38.0 38.0 40.0 27.0 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2201 1 -0.3475696533587822 2201 2 0.0987476732459669 2201 3 -0.3027142810959802 2201 4 -0.48006489058120394 2201 5 0.3133406093335367 2201 6 0.32512751516270555 2201 7 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42578.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.27952921395544 20.133342674326283 13.819999065900706 25.767129045817573 2 14.429957160699903 22.167878695911007 39.68296583361543 23.719198309773663 3 19.274675506583247 24.904286114483146 30.126529087683256 25.694509291250352 4 12.697263983565227 18.921001027173407 36.0747968998039 32.30693808945747 5 13.444299187599215 36.68176300308152 34.74063871509945 15.133299094219815 6 30.913250536931553 38.37893360724624 16.314548510598563 14.393267345223643 7 25.639648893454105 33.94808105331964 20.843449435054627 19.56882061817163 8 24.330002801382015 36.374778223923805 19.937669250163413 19.357549724530767 9 23.875945466430107 18.13778130544402 21.708376132225233 36.27789709590065 10 15.477635633579231 30.28410682603418 31.78751517415258 22.450742366234007 11 31.285600896442244 24.8856102343823 24.510925389858997 19.317863479316465 12 22.77406854047997 26.62596881128023 29.967784106826034 20.632178541413765 13 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19.05056494537305 26 20.103887008287614 27.42500291817439 33.089763044239525 19.38134702929847 27 19.169623681015967 28.69315528994304 32.591745260995424 19.54547576804557 28 18.700392193482116 27.361331590251194 32.629097021197126 21.309179195069568 29 19.678541413764123 27.306471192454946 32.483191707909235 20.531795685871696 30 19.80459220003035 26.94619865292353 33.86251415364143 19.386694993404696 31 20.20730226911943 27.056681296106078 33.412316742926514 19.323699691847978 32 20.620506116350732 27.228265944532637 32.25674666168643 19.8944812774302 33 19.231487533850032 27.87842002054347 32.34428984965916 20.545802595947336 34 19.566486133159025 28.86007096834438 32.47268652535251 19.100756373144083 35 21.37804650294145 27.49322999346344 31.73498926136894 19.393734242226166 36 19.657531048650668 28.58110000933794 32.18437762629564 19.576991315715752 37 19.806938089457464 28.192408254739004 31.743159958913065 20.257493696890467 38 19.299187599215614 27.64263703427024 32.77850406200392 20.279671304510224 39 19.766084601736857 27.5539266037912 31.52138388271547 21.158604911756466 40 20.685871696703707 26.71001027173406 32.645438416285366 19.95867961527687 41 20.584321598655336 26.713511999252965 31.03931272761229 21.66285367447941 42 20.849285647586143 27.62396115416939 31.271593986366607 20.25515921187786 43 19.453263610047625 27.448874778223924 32.41082267251844 20.68703893921001 44 19.329535904379494 28.95345036884863 30.685638248202444 21.031375478569426 45 19.69488280885237 27.9647959660099 31.46769072742553 20.872630497712205 46 20.740732094499954 27.797880287608557 30.364646558969095 21.0967410589224 47 19.964515827808384 27.929778690820804 29.956111681763005 22.149593799607807 48 19.13694089083948 28.61495004202073 30.027313474647492 22.220795592492294 49 20.98702026332991 28.08152021664021 29.66663554019983 21.264823979830048 50 19.39490148473247 29.098188439630217 30.3319637687926 21.17494630684471 51 19.08369324150811 29.075522353215828 29.240107388817556 22.600677016458505 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 462.0 1 270.0 2 78.0 3 127.5 4 177.0 5 202.0 6 227.0 7 223.5 8 220.0 9 289.0 10 358.0 11 502.5 12 647.0 13 927.0 14 1207.0 15 1317.5 16 1428.0 17 1278.5 18 1129.0 19 1080.0 20 1031.0 21 1033.0 22 1035.0 23 998.5 24 962.0 25 1015.5 26 1219.5 27 1370.0 28 1589.5 29 1809.0 30 2266.0 31 2723.0 32 3106.0 33 3489.0 34 3969.0 35 4449.0 36 4782.5 37 5116.0 38 5199.0 39 5282.0 40 5348.5 41 5415.0 42 5453.0 43 5491.0 44 5436.5 45 5382.0 46 5468.5 47 5555.0 48 5381.5 49 5208.0 50 5130.0 51 5052.0 52 5288.0 53 5524.0 54 4693.0 55 3862.0 56 3452.5 57 3043.0 58 2676.5 59 2310.0 60 1990.0 61 1670.0 62 1410.0 63 1150.0 64 950.5 65 751.0 66 591.0 67 431.0 68 343.0 69 255.0 70 195.0 71 135.0 72 104.0 73 73.0 74 62.0 75 36.0 76 21.0 77 16.0 78 11.0 79 8.5 80 6.0 81 4.0 82 2.0 83 2.5 84 3.0 85 2.5 86 2.0 87 1.5 88 1.0 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03268279017648707 2 0.0035017275189093288 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.004668970025212438 17 0.0 18 0.0035017275189093288 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.002334485012606219 27 0.0 28 0.0 29 0.0 30 0.005836212531515548 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.002334485012606219 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 85672.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 83.62000465834932 #Duplication Level Percentage of deduplicated Percentage of total 1 90.66801206048949 75.81659590861403 2 7.277161301312543 12.170325238306285 3 1.2496086270273725 3.1347683763942715 4 0.37275930237862526 1.2468053840537472 5 0.17350699226642677 0.7254327750787394 6 0.059459073708479596 0.29831808122905196 7 0.05606502316545652 0.3281710248786156 8 0.03912403343921936 0.26172414867527494 9 0.013638917085513645 0.1026437679202942 >10 0.07119977156812408 1.1217432610648161 >50 0.006955481621892738 0.4407962288232945 >100 0.009729547654678579 2.303606147400839 >500 0.0027798682821418364 2.049069657560756 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACCTTGCAGAATCTCGTATGCCGTC 987 1.1520683537211691 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 776 0.905780184891213 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 492 0.5742833131011299 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 449 0.5240918853300962 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 358 0.4178728172565132 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 306 0.3571762069287515 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 132 0.15407601083201045 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 125 0.1459053132878887 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 120 0.14006910075637313 No Hit TTATACACATCTCCGAGCCCACGAGACCTTGCAGAATCTCGTATGCCGTCT 96 0.11205528060509852 RNA PCR Primer, Index 40 (95% over 21bp) GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 86 0.10038285554206741 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0 0.0 0.0 2 0.0 0.0 0.0 3 0.0 0.0 0.0 4 0.0 0.0 0.0 5 0.0 0.0 0.0 6 0.0 0.0 0.0 7 0.0 0.0 0.0 8 0.0 0.0 0.0 9 0.0 0.0 0.0 10 0.0 0.0 0.0011672425063031094 11 0.0 0.0 0.0011672425063031094 12 0.0 0.0 0.0011672425063031094 13 0.0 0.0 0.0011672425063031094 14 0.0 0.0 0.0011672425063031094 15 0.0 0.0 0.0011672425063031094 16 0.0 0.0 0.0011672425063031094 17 0.0 0.0 0.002334485012606219 18 0.0 0.0 0.0035017275189093288 19 0.0 0.0 0.0035017275189093288 20 0.0 0.0 0.0035017275189093288 21 0.0 0.0 0.0035017275189093288 22 0.0 0.0 0.004668970025212438 23 0.0 0.0 0.012839667569334206 24 0.0 0.0 0.016341395088243532 25 0.0 0.0 0.016341395088243532 26 0.0 0.0 0.022177607619759083 27 0.0 0.0 0.02684657764497152 28 0.0 0.0 0.04085348772060883 29 0.0 0.0 0.07003455037818657 30 0.0 0.0 0.10621906807358297 31 0.0 0.0 0.18092258847698198 32 0.0 0.0 0.3046502941451116 33 0.0 0.0 0.4540573349519096 34 0.0 0.0 0.6279764683910729 35 0.0 0.0 0.7913904192735083 36 0.0 0.0 0.9874871603324307 37 0.0 0.0 1.2921374544775424 38 0.0 0.0 1.6960033616584183 39 0.0 0.0 2.1570641516481466 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTATGC 115 0.0 41.086426 41 TCCGAGC 115 0.0 41.086426 13 CCCACGA 115 0.0 41.086426 19 GTATGCC 115 0.0 41.086426 42 TCGTATG 115 0.0 41.086426 40 CGAGCCC 115 0.0 41.086426 15 CCACGAG 115 0.0 41.086426 20 GCCCACG 120 0.0 39.374493 18 CACGAGA 120 0.0 39.374493 21 TATGCCG 120 0.0 39.374493 43 TCTCGTA 120 0.0 39.374493 38 CGAGACC 120 0.0 39.374493 23 ACGAGAC 120 0.0 39.374493 22 TGCCGTC 120 0.0 39.374493 45 ATCTCGT 125 0.0 37.799507 37 CTCGTAT 125 0.0 37.799507 39 CCGAGCC 125 0.0 37.799507 14 GAGCCCA 125 0.0 37.799507 16 ACCTTGC 125 0.0 37.799507 27 CCTTGCA 125 0.0 37.799507 28 >>END_MODULE