FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 36h_25.34100000005d11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 36h_25.34100000005d11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences85672
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTTGCAGAATCTCGTATGCCGTC9871.1520683537211691No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7760.905780184891213No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4920.5742833131011299No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4490.5240918853300962No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3580.4178728172565132No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3060.3571762069287515No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1320.15407601083201045No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1250.1459053132878887No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1200.14006910075637313No Hit
TTATACACATCTCCGAGCCCACGAGACCTTGCAGAATCTCGTATGCCGTCT960.11205528060509852RNA PCR Primer, Index 40 (95% over 21bp)
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT860.10038285554206741No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC1150.041.08642641
TCCGAGC1150.041.08642613
CCCACGA1150.041.08642619
GTATGCC1150.041.08642642
TCGTATG1150.041.08642640
CGAGCCC1150.041.08642615
CCACGAG1150.041.08642620
GCCCACG1200.039.37449318
CACGAGA1200.039.37449321
TATGCCG1200.039.37449343
TCTCGTA1200.039.37449338
CGAGACC1200.039.37449323
ACGAGAC1200.039.37449322
TGCCGTC1200.039.37449345
ATCTCGT1250.037.79950737
CTCGTAT1250.037.79950739
CCGAGCC1250.037.79950714
GAGCCCA1250.037.79950716
ACCTTGC1250.037.79950727
CCTTGCA1250.037.79950728
Produced by FastQC (version 0.11.2)