FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 36h_24.34100000005d04.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 36h_24.34100000005d04.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22178
Sequences flagged as poor quality0
Sequence length51
%GC33

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTTGCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT12935.830101902786546No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTC9604.328613941744071No Hit
GCGTTGATACCACTGCTTGCAAGCAGTGGTATCAACGCAGAGTACTTTTTT6032.7189106321579946No Hit
CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3461.5601046081702588No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2881.2985841825232212No Hit
GATACCACTGCTTGCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTT2541.1452791054197853No Hit
ATACCACTGCTTGCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTT2431.0956804040039678No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2241.0100099197402832No Hit
CTGCTTGCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTT2190.9874650554603662No Hit
TGATACCACTGCTTGCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTT1960.8837586797727478No Hit
GCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1400.631256199837677No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1310.5906754441338263No Hit
TGCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT1280.5771485255658761No Hit
CTTGCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT1260.5681305798539092No Hit
GCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1160.5230408512940752No Hit
ACTCTGCGTTGATACCACTGCTTGCAAGCAGTGGTATCAACGCAGAGTACT1070.48246009559022457No Hit
TTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTCT1070.48246009559022457RNA PCR Primer, Index 27 (95% over 21bp)
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1040.4689331770222743No Hit
GTTGATACCACTGCTTGCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTT1000.45089728559834075No Hit
CCACTGCTTGCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTT990.44638831274235724No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT900.40580755703850657No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT850.3832626927585896No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAA830.3742447470466228No Hit
AAAGTACTCTGCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAA740.33366399134277214No Hit
GCTTGCAAGCAGTGGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACAC710.3201370727748219No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAA680.30661015420687165No Hit
AAAGTACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAA620.2795563170709712No Hit
ACTCTGCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAA620.2795563170709712No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT610.27504734421498783No Hit
GCGTTGATACCACTGCTTGCAAGCAGTGGTATCCTGTCTCTTATACACATC600.27053837135900444No Hit
CTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGTC570.2570114527910542No Hit
ACTCTGCGTTGATACCACTGCTTGCAAGCAGTGGTATCAACGCCTGTCTCT560.2525024799350708No Hit
GCTTGCAAGCAGTGGTATCTGTCTCTTATACACATCTCCGAGCCCACGAGA560.2525024799350708No Hit
GCGTTGATACCACTGCTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA550.2479935070790874No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTGCAAGCCTGTCTCTTATACAC500.22544864279917037No Hit
GCGTTGATACCACTGCTTGCAAGCAGTGGTATCTGTCTCTTATACACATCT490.22093966994318695No Hit
GCTTGCAAGCAGTGGTATCAACGCAGAGTACTTTCTGTCTCTTATACACAT480.21643069708720356No Hit
GCGTTGATACCACTGCTTGCAAGCAGTGGTATCACTGTCTCTTATACACAT440.19839480566326992No Hit
ACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAAAAAAA430.1938858328072865No Hit
GATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA410.18486788709531968No Hit
TTGATACCACTGCTTGCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTT400.18035891423933628No Hit
GCTTGCAAGCAGTGGTATCACTGTCTCTTATACACATCTCCGAGCCCACGA390.1758499413833529No Hit
GATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA390.1758499413833529No Hit
ATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA390.1758499413833529No Hit
GCTTGCGCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTT380.17134096852736946No Hit
AAAGTACTCTGCGTTGATACCACTGCTTGCAAGCAGTGGTATCCTGTCTCT380.17134096852736946No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTGCAAGCAGTGGTATCTGTCTC370.16683199567138607No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT360.16232302281540265No Hit
ATACCACTGCTTGCAAGCAGTGGTATCAACGCAGAGTACTTTTTCTGTCTC350.15781404995941925No Hit
CTGCGTTGATACCACTGCTTGCAAGCAGTGGTATCAACGCAGAGTACTTTT350.15781404995941925No Hit
GCTTGCAAGCAGTGGTATCCTGTCTCTTATACACATCTCCGAGCCCACGAG350.15781404995941925No Hit
TACCACTGCTTGCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTT340.15330507710343583No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTGCAAGCAGTGGTATCCTGTCT340.15330507710343583No Hit
CACTGCTTGCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTT330.14879610424745243No Hit
GCGTTGATACCACTGCTTGCGCAAGCAGTGGTATCAACGCAGAGTACTTTT320.14428713139146904No Hit
CTTATACACATCTCCGAGCCCACGAGACGTCTTAGTATCTCGTATGCCGTC320.14428713139146904No Hit
AAAGTACTCTGCGTTGATACCACTGCTTGCAAGCCTGTCTCTTATACACAT320.14428713139146904No Hit
CAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT320.14428713139146904No Hit
TGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA310.1397781585354856No Hit
ATACCACTGCTTGCAAGCAGTGGTATCAACGCCTGTCTCTTATACACATCT300.13526918567950222No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTGGAAAAAAAAAAAAAAAAAAA300.13526918567950222No Hit
AAAGTACTCTGCGTTGATACCACTGCTTGCAAGCAGTGGTATCAACGCCTG300.13526918567950222No Hit
GATACCACTGCTTGCAAGCAGTGGTATCAACGCAGAGTACTTTCTGTCTCT300.13526918567950222No Hit
CGTTGATACCACTGCTTGCAAGCAGTGGTATCAACGCAGAGTACTTTTTTT290.1307602128235188No Hit
CTCTGCGTTGATACCACTGCTTGCAAGCAGTGGTATCAACGCAGAGTACTT290.1307602128235188No Hit
GTTGATACCACTGCTTGCAAGCCTGTCTCTTATACACATCTCCGAGCCCAC290.1307602128235188No Hit
TACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA280.1262512399675354No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTGCAAGCAGTGGTATCAACGCA270.12174226711155198No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTGCAAGCAGTGGTATCAACGCC260.11723329425556857No Hit
GATACCACTGCTTGCGCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTT260.11723329425556857No Hit
GCTTGCAAGCAGTGGTATCAACCTGTCTCTTATACACATCTCCGAGCCCAC260.11723329425556857No Hit
ATACCACTGCTTGCAAGCAGTGGTATCAACGCAGAGTACTTTCTGTCTCTT250.11272432139958519No Hit
AAAGTACTCTGCGTTGATACCACTGCTTGCAAGCAGTGGTATCACTGTCTC250.11272432139958519No Hit
ATACCACTGCTTGCAAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGA250.11272432139958519No Hit
CCACTGCTTCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA230.10370637568761837No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGTC230.10370637568761837No Hit
AAAGTACTCTGCGTTGATACCACTGCTTGCAAGCAGTGGTATCAACGCAGA230.10370637568761837No Hit
GTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA230.10370637568761837No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCTCG1650.043.63636436
CCTATCT1600.043.5937534
CTATCTC1600.043.5937535
TCCTATC1450.043.4482833
GATCCTA1450.043.4482831
CTAGATC1450.043.4482828
ACTAGAT1450.043.4482827
AGACTAG1450.043.4482825
AGATCCT1450.043.4482830
TAGATCC1450.043.4482829
GACTAGA1450.043.4482826
GTATGCC1700.042.3529442
ATCTCGT1700.042.3529437
TGCCGTC1700.042.3529445
TATGCCG1700.042.3529443
TCTCGTA1700.042.3529438
ATGCCGT1700.042.3529444
CGTATGC1700.042.3529441
TCGTATG1700.042.3529440
CTCGTAT1700.042.3529439
Produced by FastQC (version 0.11.2)