##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename H23NVBCXX l02n01 36h_2.34100000005b8d.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 448154 Sequences flagged as poor quality 0 Sequence length 51 %GC 35 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.55559249722194 38.0 38.0 38.0 32.0 38.0 2 36.44832579872097 38.0 38.0 38.0 32.0 38.0 3 36.432681622835005 38.0 38.0 38.0 32.0 38.0 4 36.477601449501734 38.0 38.0 38.0 32.0 38.0 5 36.45586784899834 38.0 38.0 38.0 32.0 38.0 6 38.44463510311188 40.0 38.0 40.0 38.0 40.0 7 38.39858843165519 40.0 38.0 40.0 38.0 40.0 8 38.360938427415576 40.0 38.0 40.0 38.0 40.0 9 38.392664128848565 40.0 38.0 40.0 38.0 40.0 10 38.35845490612602 40.0 38.0 40.0 38.0 40.0 11 38.25617756396239 40.0 38.0 40.0 38.0 40.0 12 38.25991734983956 40.0 38.0 40.0 38.0 40.0 13 38.16510395979953 40.0 38.0 40.0 38.0 40.0 14 38.09989423278605 40.0 38.0 40.0 38.0 40.0 15 38.07820972255073 40.0 38.0 40.0 32.0 40.0 16 38.047144508360965 40.0 38.0 40.0 32.0 40.0 17 38.1752991159289 40.0 38.0 40.0 38.0 40.0 18 38.08319238476059 40.0 38.0 40.0 32.0 40.0 19 38.16366025964289 40.0 38.0 40.0 38.0 40.0 20 38.174926476166675 40.0 38.0 40.0 38.0 40.0 21 38.13871347795624 40.0 38.0 40.0 38.0 40.0 22 38.19778469008421 40.0 38.0 40.0 38.0 40.0 23 38.11235646675027 40.0 38.0 40.0 32.0 40.0 24 38.05689338932599 40.0 38.0 40.0 32.0 40.0 25 37.97378579684662 40.0 38.0 40.0 32.0 40.0 26 37.73031368681301 40.0 38.0 40.0 32.0 40.0 27 37.48260642546982 40.0 38.0 40.0 32.0 40.0 28 37.21878193656645 40.0 38.0 40.0 32.0 40.0 29 36.86354913712697 40.0 38.0 40.0 32.0 40.0 30 36.36817254782954 40.0 38.0 40.0 27.0 40.0 31 35.764538529166316 40.0 38.0 40.0 27.0 40.0 32 35.04302315721828 38.0 32.0 40.0 13.0 40.0 33 34.27682671581644 38.0 32.0 40.0 13.0 40.0 34 33.53386782222182 38.0 32.0 40.0 13.0 40.0 35 32.6922419525431 38.0 27.0 40.0 13.0 40.0 36 31.81795766633791 38.0 27.0 40.0 13.0 40.0 37 31.02808409609197 38.0 27.0 40.0 13.0 40.0 38 30.288398630827796 38.0 27.0 40.0 13.0 40.0 39 29.633291234709496 32.0 13.0 38.0 13.0 40.0 40 29.072562110346 32.0 13.0 38.0 13.0 40.0 41 28.655598298799074 32.0 13.0 38.0 13.0 40.0 42 28.310183552975094 32.0 13.0 38.0 13.0 40.0 43 27.95943581893724 32.0 13.0 38.0 13.0 40.0 44 27.62102536181759 32.0 13.0 38.0 13.0 40.0 45 27.43203006109507 32.0 13.0 38.0 13.0 40.0 46 27.281363102861963 27.0 13.0 38.0 13.0 40.0 47 27.15348518589592 27.0 13.0 38.0 13.0 40.0 48 26.955278765781404 27.0 13.0 38.0 13.0 40.0 49 26.805292377173917 27.0 13.0 38.0 13.0 40.0 50 26.726727865867538 27.0 13.0 38.0 13.0 40.0 51 25.37914422274486 27.0 13.0 38.0 13.0 38.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2201 1 0.361948328020155 2201 2 0.18305506327352816 2201 3 0.17565690843829174 2201 4 0.33746363987799555 2201 5 0.4525091308925582 2201 6 0.2786540257238599 2201 7 0.4718718915030067 2201 8 0.27866013469616746 2201 9 0.45295821576459616 2201 10 0.407688700924993 2201 11 0.5215646856803531 2201 12 0.5420808207848467 2201 13 0.1319877949217627 2201 14 0.3816603805118177 2201 15 0.19945638143911282 2201 16 0.35509406766983176 2201 17 0.493237978714518 2201 18 0.4836299221668483 2201 19 0.40917547186238323 2201 20 0.17406120980468387 2201 21 0.35977010408903 2201 22 0.2719997976542743 2201 23 0.2441853372929117 2201 24 0.20227172490069734 2201 25 0.01821652917535488 2201 26 0.02665662899676846 2201 27 -0.09671465837545412 2201 28 -0.21610093719342416 2201 29 -0.456287669707784 2201 30 -0.08678547827986449 2201 31 -0.17956051248565075 2201 32 0.266204797762839 2201 33 0.05605907461546167 2201 34 -0.3938978588259019 2201 35 -1.065298568996539 2201 36 -1.3081706734714054 2201 37 -0.8456995714863638 2201 38 -1.0599676313161233 2201 39 -0.6495358829095785 2201 40 -1.0074363223465141 2201 41 -1.2575274390429811 2201 42 -1.6871211024663602 2201 43 -0.9891686533350068 2201 44 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1213 41 2.285421278905737 1213 42 2.389374624029365 1213 43 2.95378006461371 1213 44 2.8582321384945715 1213 45 2.6839216796514265 1213 46 2.712107645762565 1213 47 2.3012962105806913 1213 48 2.1851345744191626 1213 49 1.9982092039867965 1213 50 2.1555761563987517 1213 51 1.5687454356063917 >>END_MODULE >>Per sequence quality scores pass #Quality Count 14 4.0 15 8.0 16 15.0 17 16.0 18 20.0 19 35.0 20 90.0 21 307.0 22 704.0 23 1525.0 24 2849.0 25 4660.0 26 7419.0 27 11046.0 28 14782.0 29 18990.0 30 24866.0 31 30758.0 32 36211.0 33 38291.0 34 38863.0 35 38369.0 36 37449.0 37 39548.0 38 48111.0 39 53218.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 52.0961866411907 21.956500388389387 14.019964107463325 11.927348862956581 2 9.647813309590912 27.82616263112444 31.46964250250478 31.056381556779865 3 36.866345051031566 32.82063754869978 24.1356765754629 6.177340824805759 4 17.572754008666664 16.82323487015624 36.86768387652459 28.73632724465251 5 9.394315346956626 28.875341958344674 42.442106954305885 19.28823574039281 6 40.15204594849092 32.28934696555202 7.529331435176301 20.029275650780757 7 7.64714807856228 42.51328784301825 26.081436291988915 23.758127786430556 8 21.50555389442022 20.32113960825966 31.222079910030924 26.95122658728919 9 18.06700375317413 30.181143089205943 23.57537810663299 28.176475050986937 10 13.153737331363772 22.60361393628083 27.040704757739526 37.20194397461587 11 18.647271201795842 24.40700128975825 14.648797490103671 42.29693001834224 12 21.36475408007069 38.08891586374327 25.732672251056556 14.813657805129488 13 24.171824863774503 19.605091107074802 23.128433529545646 33.094650499605045 14 20.033068989677655 26.463447832664666 33.905309335630164 19.598173842027517 15 35.653815429517536 19.098345657965787 26.83296366873887 18.41487524377781 16 7.846361883680246 37.36901531650541 21.41186761398122 33.37275518583313 17 17.712477658299733 33.41001845351922 32.01830624808938 16.859197640091665 18 10.889502787087906 26.23968509700137 47.66634384943924 15.204468266471489 19 10.67244741762876 41.21596594027946 33.50053776157303 14.611048880518752 20 22.52596205768553 25.955363558062633 35.98495160145843 15.533722782793417 21 3.180156821092749 38.51622433359962 39.991386889328226 18.31223195597942 22 6.907893268831697 44.328958349138915 38.769485489363035 9.99366289266636 23 3.5902836971219716 37.86644769431937 45.36811006930653 13.175158539252132 24 5.097354927100952 43.77624655810279 44.98409029039125 6.142308224405004 25 9.328712897798525 38.89087233406374 42.68019475448172 9.10022001365602 26 6.312576285331126 39.02238744357396 46.00861759265251 8.656418678442407 27 8.63163109109815 41.19923062161668 45.77288164336367 4.396256643921509 28 6.868174779205362 40.23884646795521 45.1179728396935 7.775005913145927 29 8.236231295492175 39.08634085604502 44.71565577904024 7.961772069422565 30 10.384524409891597 38.24737022970603 45.2025938438606 6.1655115165417795 31 11.110020216264944 36.47384604399381 40.89152389580367 11.524609843937576 32 13.23406686094512 37.058466509280294 40.73510445070222 8.972362179072373 33 14.421158798091726 36.97478991596639 37.1164822806446 11.487569005297287 34 14.715700406556675 37.4837667408971 35.929167205915824 11.8713656466304 35 18.31981863377321 36.36562431664115 33.26557388754758 12.048983162038049 36 18.390106972156893 34.54147458239801 32.351602350977565 14.716816094467527 37 19.490175252257036 34.95985754896754 30.223985504982664 15.325981693792759 38 21.212127973866128 33.38339053093357 28.44580211266663 16.95867938253368 39 21.277284147859888 32.401585169383736 28.373282398461242 17.94784828429513 40 23.7864662593662 30.091441781173433 28.481057850649556 17.64103410881081 41 24.56521642114095 29.72527300883179 27.96873396198628 17.74077660804099 42 26.184079579787305 28.53282576971309 27.580251431427588 17.702843219072015 43 25.906719565149476 28.122698893683868 27.38879938592537 18.581782155241278 44 25.520245272830323 28.474363723184442 28.246763389370617 17.758627614614618 45 24.888765915288047 26.50562083569487 28.929564390812086 19.676048858204993 46 23.99130655979864 27.82414080873985 29.184610647232873 18.999941984228634 47 23.556070261875437 27.46456559381638 28.00545350684589 20.97391063746229 48 21.36413233873253 29.123312797192032 27.77667448393741 21.735880380138035 49 21.13291413219563 28.69616247986183 27.76813327561508 22.40279011232746 50 18.635599895571378 28.274049264425322 28.390081066064493 24.70026977393881 51 18.834347888178797 28.14273983362491 26.686734621014672 26.33617765718162 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 8.0 1 7.5 2 7.0 3 17.0 4 27.0 5 30.0 6 33.0 7 33.0 8 33.0 9 38.0 10 43.0 11 77.0 12 111.0 13 214.5 14 318.0 15 466.5 16 615.0 17 765.5 18 916.0 19 1173.0 20 1430.0 21 1984.5 22 2539.0 23 3490.0 24 4441.0 25 5604.5 26 10616.5 27 14465.0 28 20222.0 29 25979.0 30 31856.0 31 37733.0 32 45809.0 33 53885.0 34 65170.5 35 76456.0 36 81453.0 37 86450.0 38 77645.0 39 68840.0 40 50469.5 41 32099.0 42 23618.0 43 15137.0 44 11046.5 45 6956.0 46 6752.5 47 6549.0 48 4554.5 49 2560.0 50 2822.5 51 3085.0 52 1801.5 53 518.0 54 283.5 55 49.0 56 35.5 57 22.0 58 22.0 59 22.0 60 20.0 61 18.0 62 14.0 63 10.0 64 8.5 65 7.0 66 8.0 67 9.0 68 6.5 69 4.0 70 3.0 71 2.0 72 2.0 73 2.0 74 3.0 75 3.5 76 3.0 77 1.5 78 0.0 79 0.5 80 1.0 81 0.5 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03347063732556219 2 0.002454513403874561 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.001785100657363317 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0022313758217041464 17 2.2313758217041462E-4 18 0.003570201314726634 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 2.2313758217041462E-4 27 0.0 28 0.0 29 0.0 30 0.003570201314726634 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 4.4627516434082924E-4 48 2.2313758217041462E-4 49 0.0 50 2.2313758217041462E-4 51 0.0022313758217041464 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 448154.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 33.38464995245998 #Duplication Level Percentage of deduplicated Percentage of total 1 85.80311776627548 28.645070514568072 2 6.27278491587601 4.188294572871835 3 2.318730361782584 2.3223000438675734 4 1.223828853096718 1.6342839144941803 5 0.8232869916734344 1.3742574013715725 6 0.5336724739691315 1.0689881239633663 7 0.3445777603639225 0.8052525537806535 8 0.31189129174566865 0.8329905278519776 9 0.2257826991156507 0.6783908737767836 >10 1.632439543052167 10.889594859497304 >50 0.1681632285730124 3.860325728629509 >100 0.26539351883892814 18.772985322515133 >500 0.053043295418972326 12.295619754133371 >1k 0.023287300218204566 12.631645808678716 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAGTACTCTGCGTTGATAC 4448 0.9925159654940042 No Hit GCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAGTACTCTGCGTTGATA 3871 0.8637655805816751 No Hit GCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAGTACTCTGCGTTGAT 3015 0.6727598102438002 No Hit GCGTTGATACCACTGCTTCGCAAAAAAAAAAAAGTACTCTGCGTTGATACC 2722 0.6073804986678686 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTGCGAAGCAGTGGTATCAACGC 2441 0.5446788380779821 No Hit GCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAGTACTCTGCGTTGA 2429 0.5420011870919371 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTGCGAAGCAGTGGTATCAACG 2110 0.4708202983795749 No Hit GCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAGTACTCTGCGTTG 1861 0.4152590404191417 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTGCGAAGCAGTGGTATCAA 1714 0.3824578158400907 No Hit AAAAAGTACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAA 1617 0.36081347036956046 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTGCGAAGCAGTGGTATCA 1546 0.344970702035461 No Hit CTTATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCGTC 1518 0.3387228497346894 RNA PCR Primer, Index 27 (95% over 21bp) GTATCAACGCAGAGTACTTTTTTTTTTTTTTTGCGAAGCAGTGGTATCAAC 1512 0.3373840242416669 No Hit GCGTTGATACCACTGCTTCAAAAAAAAGTACTCTGCGTTGATACCACTGCT 1484 0.3311361719408953 No Hit GCGTTGATACCACTGCTTCGAAAAAAAAAAAAAAAGTACTCTGCGTTGATA 1482 0.33068989677655447 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTGCGAAGCAGTGGTATCAACGCA 1466 0.32711969546182784 No Hit TACCACTGCTTCGCAAAAAAAAAAAAAGTACTCTGCGTTGATACCACTGCT 1292 0.2882937561641757 No Hit GCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAGTACTCTGCGTT 1280 0.28561610517813074 No Hit ACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAGTACTCTGCGTT 1251 0.2791451152951887 No Hit GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTGCGAAGCAGTGGTAT 1248 0.2784757025486775 No Hit GCGTTGATACCACTGCTTCGAAAAAAAAAAAAAAGTACTCTGCGTTGATAC 1242 0.277136877055655 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTGCGAAGCAGTGGTATCAA 1211 0.27021961200837213 No Hit GTATCAACGCAGAGTACTTTTTGCAAGCAGTGGTATCAACGCAGAGTACTT 1210 0.2699964744262017 No Hit CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTGCGAAGCAGTGGTATC 1188 0.2650874476184526 No Hit TACCACTGCTTCGCAAAAAAAAAAAAAAGTACTCTGCGTTGATACCACTGC 1171 0.2612941087215555 No Hit GATACCACTGCTTCGCAAAAAAAAAAAAAGTACTCTGCGTTGATACCACTG 1168 0.2606246959750443 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTGCGAAGCAGTGGTATCAAC 1160 0.25883959531768097 No Hit GCGTTGATACCACTGCTTGCAAAAAGTACTCTGCGTTGATACCACTGCTTG 1135 0.25326115576342056 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTGCGAAGCAGTGGTATCAACG 1129 0.2519223302703981 No Hit ACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAGTACTCTGCGT 1128 0.2516991926882277 No Hit CAGTGGTATCAACGCAGAGTACTTTTTGCAAGCAGTGGTATCAACGCAGAG 1124 0.2508066423595461 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTGCGAAGCAGTGGTATC 1099 0.2452282028052857 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTGCGAAGCAGTGGTATCAACGCA 1071 0.2389803505045141 No Hit GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTGCGAAGCAGTGGTA 1052 0.2347407364432762 No Hit GATACCACTGCTTCGCAAAAAAAAAAAAAAGTACTCTGCGTTGATACCACT 1049 0.23407132369676495 No Hit CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTGCGAAGCAGTGGTAT 1048 0.23384818611459451 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTGCGAAGCAGTGGTATCAACGC 972 0.21688972986964303 No Hit GTATCAACGCAGAGTACTTTTTTTTGAAGCAGTGGTATCAACGCAGAGTAC 946 0.21108815273321227 No Hit TGATACCACTGCTTCGCAAAAAAAAAAAAAGTACTCTGCGTTGATACCACT 936 0.2088567769115081 No Hit GCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAGTACTCTGCGT 902 0.201270099117714 No Hit TACCACTGCTTCGCAAAAAAAAAAAAAAAGTACTCTGCGTTGATACCACTG 898 0.20037754878903233 No Hit CCACTGCTTCGCAAAAAAAAAAAAAAGTACTCTGCGTTGATACCACTGCTT 885 0.19747676022081698 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTGCGAAGCAGTGGTATCAAC 883 0.19703048505647613 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTGCGAAGCAGTGGTATCA 859 0.19167518308438616 No Hit GTGGTATCAACGCAGAGTACTTTTTGCAAGCAGTGGTATCAACGCAGAGTA 857 0.19122890792004535 No Hit ACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAGTACTCTGCG 857 0.19122890792004535 No Hit GCGTTGATACCACTGCTTCGAAAAAAAAAAAAAAAAGTACTCTGCGTTGAT 850 0.18966694484485244 No Hit TGATACCACTGCTTCGCAAAAAAAAAAAAAAGTACTCTGCGTTGATACCAC 849 0.189443807262682 No Hit AAAGTACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAA 842 0.18788184418748913 No Hit GCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAGTACTCTGCGTTGATA 834 0.18609674353012579 No Hit ACGCAGAGTACTTTTTTTTTTTTTGCGAAGCAGTGGTATCAACGCAGAGTA 833 0.18587360594795538 No Hit CAGTGGTATCAACGCAGAGTACTTTTTTTTGAAGCAGTGGTATCAACGCAG 829 0.18498105561927375 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTGCGAAGCAGTGGTAT 828 0.1847579180371033 No Hit CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTGCGAAGCAGTGGTATCA 827 0.1845347804549329 No Hit GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTGCGAAGCAGTGGTATC 814 0.18163399188671753 No Hit GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTGCGAAGCAGTGGT 811 0.18096457914020628 No Hit CCACTGCTTCGCAAAAAAAAAAAAAGTACTCTGCGTTGATACCACTGCTTC 808 0.18029516639369503 No Hit GATACCACTGCTTCGCAAAAAAAAAAAAGTACTCTGCGTTGATACCACTGC 792 0.1767249650789684 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTCGAAGCAGTGGTATCAACG 791 0.17650182749679796 No Hit ACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAGTACTCTGCGTTG 782 0.17449358925726424 No Hit AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTGCGAAGCAGTGGT 780 0.17404731409292343 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTGAAGCAGTGGTATCAACGCAGAG 762 0.17003083761385596 No Hit GCGTTGATACCACTGCTTGCAAAAAGTACTCTGCGTTGATACCACTGCTTC 759 0.1693614248673447 No Hit TACCACTGCTTCGCAAAAAAAAAAAAGTACTCTGCGTTGATACCACTGCTT 757 0.16891514970300386 No Hit GCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAGTACTCTGCGTTGAT 748 0.16690691146347014 No Hit AAAAAGTACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAGTACT 743 0.16579122355261808 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTGCGAAGCAGTGGTATCAACGCAG 739 0.1648986732239364 No Hit GCGTTGATACCACTGCTTCGCAAAAAAAAAAAGTACTCTGCGTTGATACCA 738 0.164675535641766 No Hit CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTGCGAAGCAGTGGTA 733 0.16355984773091392 No Hit GTTGATACCACTGCTTCGCAAAAAAAAAAAAAGTACTCTGCGTTGATACCA 731 0.1631135725665731 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTCGAAGCAGTGGTATCAACGC 731 0.1631135725665731 No Hit ACGCAGAGTACTTTTTTTTTTTTTTGCGAAGCAGTGGTATCAACGCAGAGT 721 0.16088219674486895 No Hit AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTGCGAAGCAGTGG 718 0.1602127839983577 No Hit GATACCACTGCTTCGCAAAAAAAAAAAAAAAGTACTCTGCGTTGATACCAC 716 0.15976650883401688 No Hit ATACCACTGCTTGCAAAAAGTACTCTGCGTTGATACCACTGCTTGCAAAAA 714 0.15932023366967604 No Hit GCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAGTACTCTGCGTTGATAC 712 0.15887395850533523 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTGCGAAGCAGTGGTATCAACG 711 0.1586508209231648 No Hit AAAAAGTACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAGTAC 701 0.15641944510146066 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTGCGAAGCAGTGGTATCAACGC 689 0.1537417941154157 No Hit ACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAGTACTCTGC 688 0.15351865653324528 No Hit AAAGTACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAGTACTCT 671 0.14972531763634822 No Hit GTTGATACCACTGCTTCGCAAAAAAAAAAAAAAGTACTCTGCGTTGATACC 671 0.14972531763634822 No Hit CCACTGCTTCGCAAAAAAAAAAAAAAAGTACTCTGCGTTGATACCACTGCT 669 0.14927904247200738 No Hit GCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAGTACTCTGCGTTGA 657 0.1466013914859624 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTCGAAGCAGTGGTATCA 657 0.1466013914859624 No Hit TACCACTGCTTCGCAAAAAAAAAAAAAAAAGTACTCTGCGTTGATACCACT 656 0.146378253903792 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTGCGAAGCAGTGGTATCAA 653 0.14570884115728075 No Hit GCGTTGATACCACTGCTTCGAAAAAAAAAAAAAGTACTCTGCGTTGATACC 645 0.14392374049991744 No Hit ATACCACTGCTTCGCAAAAAAAAAAAAAGTACTCTGCGTTGATACCACTGC 636 0.1419155022603837 No Hit GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTGCGAAGCAGTGG 630 0.1405766767673612 No Hit AAAGTACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAGTACTC 628 0.1401304016030204 No Hit GCTTAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT 613 0.13678333787046418 No Hit ATACCACTGCTTCGCAAAAAAAAAAAAAAGTACTCTGCGTTGATACCACTG 600 0.13388254930224877 No Hit GGTATCAACGCAGAGTACTTTTTGCAAGCAGTGGTATCAACGCAGAGTACT 594 0.1325437238092263 No Hit AAAAAGTACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAGTA 582 0.12986607282318133 No Hit TGATACCACTGCTTCGCAAAAAAAAAAAAGTACTCTGCGTTGATACCACTG 577 0.12875038491232924 No Hit CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTGCGAAGCAGTGGT 564 0.12584959634411386 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTGCGAAGCAGTGGTA 560 0.1249570460154322 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTGCGAAGCAGTGGTATCAAC 559 0.12473390843326178 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTGCGAAGCAGTGGTATC 559 0.12473390843326178 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTCGAAGCAGTGGTATCAA 556 0.12406449568675053 No Hit TGATACCACTGCTTCGCAAAAAAAAAAAAAAAGTACTCTGCGTTGATACCA 546 0.1218331198650464 No Hit ACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAGTACTCTG 546 0.1218331198650464 No Hit ACGCAGAGTACTTTTTTTTTTTTGCGAAGCAGTGGTATCAACGCAGAGTAC 543 0.12116370711853515 No Hit AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTGCGAAGCAGTGGTA 538 0.12004801920768307 No Hit GCGTTGATACCACTGCTTAAGCAGTGGTATCAACGCAGAGTACTTTTTTTT 538 0.12004801920768307 No Hit GATACCACTGCTTCGCAAAAAAAAAAAAAAAAGTACTCTGCGTTGATACCA 529 0.11803978096814935 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTCGAAGCAGTGGTATCAAC 527 0.1175935058038085 No Hit AAAGTACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAGTACT 527 0.1175935058038085 No Hit ACTCTGCGTTGATACCACTGCTTGCAAAAAGTACTCTGCGTTGATACCACT 526 0.1173703682216381 No Hit GCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAAGTACTCTGCG 524 0.11692409305729726 No Hit AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTGCGAAGCAGTG 523 0.11670095547512685 No Hit TATCAACGCAGAGTACTTTTTGCAAGCAGTGGTATCAACGCAGAGTACTTT 520 0.11603154272861561 No Hit CCACTGCTTCGCAAAAAAAAAAAAAAAAGTACTCTGCGTTGATACCACTGC 517 0.11536212998210438 No Hit AAAAAGTACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAGT 506 0.1129076165782298 No Hit GTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAGTACTCTGCGTTGATAC 506 0.1129076165782298 No Hit GATACCACTGCTTCGCAAAAAAAAAAAAAAAAAGTACTCTGCGTTGATACC 504 0.11246134141388898 No Hit GTTGATACCACTGCTTCGCAAAAAAAAAAAAGTACTCTGCGTTGATACCAC 503 0.11223820383171856 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTGCGAAGCAGTGGTATCAACGCAGAG 498 0.11112251592086649 No Hit AAAAAGTACTCTGCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAA 480 0.10710603944179903 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTGCGAAGCAGTGGTATCAACGCAG 476 0.10621348911311736 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTGCAAGCAGTGGTATCAACG 474 0.10576721394877654 No Hit TGATACCACTGCTTCGCAAAAAAAAAAAAAAAAGTACTCTGCGTTGATACC 472 0.10532093878443571 No Hit CCACTGCTTCGCAAAAAAAAAAAAGTACTCTGCGTTGATACCACTGCTTCG 472 0.10532093878443571 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTGCAAGCAGTGGTATCAAC 467 0.10420525087358364 No Hit AAAAAGTACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAGTACTC 465 0.1037589757092428 No Hit GCGTTGATACCACTGCTTGCAAAAAAAAAAAAAGTACTCTGCGTTGATACC 463 0.10331270054490196 No Hit GCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAGTACTCTGCGTTG 458 0.10219701263404991 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTGCGAAGCAGTGGTATCA 454 0.10130446230536824 No Hit GTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAGTACTCTGCGTTGATA 453 0.10108132472319784 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0 0.0 0.0 2 0.0 0.0 0.0 3 0.0 0.0 2.2313758217041465E-4 4 0.0 0.0 4.462751643408293E-4 5 0.0 0.0 4.462751643408293E-4 6 0.0 0.0 4.462751643408293E-4 7 0.0 0.0 4.462751643408293E-4 8 0.0 0.0 6.694127465112439E-4 9 0.0 0.0 8.925503286816586E-4 10 0.0 0.0 0.0015619630751929024 11 0.0 0.0 0.0020082382395337317 12 0.0 0.0 0.004685889225578707 13 0.0 0.0 0.006694127465112439 14 0.0 0.0 0.008032952958134927 15 0.0 0.0 0.009371778451157414 16 0.0 0.0 0.013165117348054464 17 0.0 0.0 0.017404731409292343 18 0.0 0.0 0.027669060189131414 19 0.0 0.0 0.03994162720850422 20 0.0 0.0 0.047082029837957486 21 0.0 0.0 0.058908321692989464 22 0.0 0.0 0.0656024491581019 23 0.0 0.0 0.07073461354802144 24 0.0 0.0 0.07809815375964513 25 0.0 0.0 0.08479228122475756 26 0.0 0.0 0.08947817045033626 27 0.0 0.0 0.09327150934723331 28 0.0 0.0 0.10599035153094695 29 0.0 0.0 0.1173703682216381 30 0.0 0.0 0.13165117348054464 31 0.0 0.0 0.17315476376424174 32 0.0 0.0 0.21443521646576846 33 0.0 0.0 0.2664262731114751 34 0.0 0.0 0.3141777156959438 35 0.0 0.0 0.3764331011214895 36 0.0 0.0 0.4286472953493665 37 0.0 0.0 0.5183486033818732 38 0.0 0.0 0.6113969751469361 39 0.0 0.0 0.7359077459980274 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGCCGTC 155 0.0 40.644253 45 ATGCCGT 155 0.0 40.644253 44 GTATGCC 160 0.0 39.374123 42 TATGCCG 160 0.0 39.374123 43 TCTCGTA 160 0.0 39.374123 38 CGTATGC 160 0.0 39.374123 41 TCGTATG 160 0.0 39.374123 40 CTCGTAT 160 0.0 39.374123 39 CGAGACG 155 0.0 39.192673 23 GCTATCT 145 0.0 38.792236 34 CGAATTG 145 0.0 38.792236 28 AGACGAA 145 0.0 38.792236 25 GAATTGC 145 0.0 38.792236 29 GACGAAT 145 0.0 38.792236 26 ACGAATT 145 0.0 38.792236 27 AATTGCT 145 0.0 38.792236 30 ATCTCGT 165 0.0 38.180965 37 ACGAGAC 165 0.0 38.180965 22 TATCTCG 165 0.0 38.180965 36 CTATCTC 160 0.0 37.967903 35 >>END_MODULE