FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 36h_2.34100000005b8d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 36h_2.34100000005b8d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences448154
Sequences flagged as poor quality0
Sequence length51
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAGTACTCTGCGTTGATAC44480.9925159654940042No Hit
GCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAGTACTCTGCGTTGATA38710.8637655805816751No Hit
GCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAGTACTCTGCGTTGAT30150.6727598102438002No Hit
GCGTTGATACCACTGCTTCGCAAAAAAAAAAAAGTACTCTGCGTTGATACC27220.6073804986678686No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTGCGAAGCAGTGGTATCAACGC24410.5446788380779821No Hit
GCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAGTACTCTGCGTTGA24290.5420011870919371No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTGCGAAGCAGTGGTATCAACG21100.4708202983795749No Hit
GCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAGTACTCTGCGTTG18610.4152590404191417No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTGCGAAGCAGTGGTATCAA17140.3824578158400907No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAA16170.36081347036956046No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTGCGAAGCAGTGGTATCA15460.344970702035461No Hit
CTTATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCGTC15180.3387228497346894RNA PCR Primer, Index 27 (95% over 21bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTGCGAAGCAGTGGTATCAAC15120.3373840242416669No Hit
GCGTTGATACCACTGCTTCAAAAAAAAGTACTCTGCGTTGATACCACTGCT14840.3311361719408953No Hit
GCGTTGATACCACTGCTTCGAAAAAAAAAAAAAAAGTACTCTGCGTTGATA14820.33068989677655447No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTGCGAAGCAGTGGTATCAACGCA14660.32711969546182784No Hit
TACCACTGCTTCGCAAAAAAAAAAAAAGTACTCTGCGTTGATACCACTGCT12920.2882937561641757No Hit
GCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAGTACTCTGCGTT12800.28561610517813074No Hit
ACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAGTACTCTGCGTT12510.2791451152951887No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTGCGAAGCAGTGGTAT12480.2784757025486775No Hit
GCGTTGATACCACTGCTTCGAAAAAAAAAAAAAAGTACTCTGCGTTGATAC12420.277136877055655No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTGCGAAGCAGTGGTATCAA12110.27021961200837213No Hit
GTATCAACGCAGAGTACTTTTTGCAAGCAGTGGTATCAACGCAGAGTACTT12100.2699964744262017No Hit
CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTGCGAAGCAGTGGTATC11880.2650874476184526No Hit
TACCACTGCTTCGCAAAAAAAAAAAAAAGTACTCTGCGTTGATACCACTGC11710.2612941087215555No Hit
GATACCACTGCTTCGCAAAAAAAAAAAAAGTACTCTGCGTTGATACCACTG11680.2606246959750443No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTGCGAAGCAGTGGTATCAAC11600.25883959531768097No Hit
GCGTTGATACCACTGCTTGCAAAAAGTACTCTGCGTTGATACCACTGCTTG11350.25326115576342056No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTGCGAAGCAGTGGTATCAACG11290.2519223302703981No Hit
ACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAGTACTCTGCGT11280.2516991926882277No Hit
CAGTGGTATCAACGCAGAGTACTTTTTGCAAGCAGTGGTATCAACGCAGAG11240.2508066423595461No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTGCGAAGCAGTGGTATC10990.2452282028052857No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTGCGAAGCAGTGGTATCAACGCA10710.2389803505045141No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTGCGAAGCAGTGGTA10520.2347407364432762No Hit
GATACCACTGCTTCGCAAAAAAAAAAAAAAGTACTCTGCGTTGATACCACT10490.23407132369676495No Hit
CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTGCGAAGCAGTGGTAT10480.23384818611459451No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTGCGAAGCAGTGGTATCAACGC9720.21688972986964303No Hit
GTATCAACGCAGAGTACTTTTTTTTGAAGCAGTGGTATCAACGCAGAGTAC9460.21108815273321227No Hit
TGATACCACTGCTTCGCAAAAAAAAAAAAAGTACTCTGCGTTGATACCACT9360.2088567769115081No Hit
GCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAGTACTCTGCGT9020.201270099117714No Hit
TACCACTGCTTCGCAAAAAAAAAAAAAAAGTACTCTGCGTTGATACCACTG8980.20037754878903233No Hit
CCACTGCTTCGCAAAAAAAAAAAAAAGTACTCTGCGTTGATACCACTGCTT8850.19747676022081698No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTGCGAAGCAGTGGTATCAAC8830.19703048505647613No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTGCGAAGCAGTGGTATCA8590.19167518308438616No Hit
GTGGTATCAACGCAGAGTACTTTTTGCAAGCAGTGGTATCAACGCAGAGTA8570.19122890792004535No Hit
ACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAGTACTCTGCG8570.19122890792004535No Hit
GCGTTGATACCACTGCTTCGAAAAAAAAAAAAAAAAGTACTCTGCGTTGAT8500.18966694484485244No Hit
TGATACCACTGCTTCGCAAAAAAAAAAAAAAGTACTCTGCGTTGATACCAC8490.189443807262682No Hit
AAAGTACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAA8420.18788184418748913No Hit
GCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAGTACTCTGCGTTGATA8340.18609674353012579No Hit
ACGCAGAGTACTTTTTTTTTTTTTGCGAAGCAGTGGTATCAACGCAGAGTA8330.18587360594795538No Hit
CAGTGGTATCAACGCAGAGTACTTTTTTTTGAAGCAGTGGTATCAACGCAG8290.18498105561927375No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTGCGAAGCAGTGGTAT8280.1847579180371033No Hit
CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTGCGAAGCAGTGGTATCA8270.1845347804549329No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTGCGAAGCAGTGGTATC8140.18163399188671753No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTGCGAAGCAGTGGT8110.18096457914020628No Hit
CCACTGCTTCGCAAAAAAAAAAAAAGTACTCTGCGTTGATACCACTGCTTC8080.18029516639369503No Hit
GATACCACTGCTTCGCAAAAAAAAAAAAGTACTCTGCGTTGATACCACTGC7920.1767249650789684No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTCGAAGCAGTGGTATCAACG7910.17650182749679796No Hit
ACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAGTACTCTGCGTTG7820.17449358925726424No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTGCGAAGCAGTGGT7800.17404731409292343No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTGAAGCAGTGGTATCAACGCAGAG7620.17003083761385596No Hit
GCGTTGATACCACTGCTTGCAAAAAGTACTCTGCGTTGATACCACTGCTTC7590.1693614248673447No Hit
TACCACTGCTTCGCAAAAAAAAAAAAGTACTCTGCGTTGATACCACTGCTT7570.16891514970300386No Hit
GCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAGTACTCTGCGTTGAT7480.16690691146347014No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAGTACT7430.16579122355261808No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTGCGAAGCAGTGGTATCAACGCAG7390.1648986732239364No Hit
GCGTTGATACCACTGCTTCGCAAAAAAAAAAAGTACTCTGCGTTGATACCA7380.164675535641766No Hit
CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTGCGAAGCAGTGGTA7330.16355984773091392No Hit
GTTGATACCACTGCTTCGCAAAAAAAAAAAAAGTACTCTGCGTTGATACCA7310.1631135725665731No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTCGAAGCAGTGGTATCAACGC7310.1631135725665731No Hit
ACGCAGAGTACTTTTTTTTTTTTTTGCGAAGCAGTGGTATCAACGCAGAGT7210.16088219674486895No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTGCGAAGCAGTGG7180.1602127839983577No Hit
GATACCACTGCTTCGCAAAAAAAAAAAAAAAGTACTCTGCGTTGATACCAC7160.15976650883401688No Hit
ATACCACTGCTTGCAAAAAGTACTCTGCGTTGATACCACTGCTTGCAAAAA7140.15932023366967604No Hit
GCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAGTACTCTGCGTTGATAC7120.15887395850533523No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTGCGAAGCAGTGGTATCAACG7110.1586508209231648No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAGTAC7010.15641944510146066No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTGCGAAGCAGTGGTATCAACGC6890.1537417941154157No Hit
ACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAGTACTCTGC6880.15351865653324528No Hit
AAAGTACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAGTACTCT6710.14972531763634822No Hit
GTTGATACCACTGCTTCGCAAAAAAAAAAAAAAGTACTCTGCGTTGATACC6710.14972531763634822No Hit
CCACTGCTTCGCAAAAAAAAAAAAAAAGTACTCTGCGTTGATACCACTGCT6690.14927904247200738No Hit
GCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAGTACTCTGCGTTGA6570.1466013914859624No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTCGAAGCAGTGGTATCA6570.1466013914859624No Hit
TACCACTGCTTCGCAAAAAAAAAAAAAAAAGTACTCTGCGTTGATACCACT6560.146378253903792No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTGCGAAGCAGTGGTATCAA6530.14570884115728075No Hit
GCGTTGATACCACTGCTTCGAAAAAAAAAAAAAGTACTCTGCGTTGATACC6450.14392374049991744No Hit
ATACCACTGCTTCGCAAAAAAAAAAAAAGTACTCTGCGTTGATACCACTGC6360.1419155022603837No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTGCGAAGCAGTGG6300.1405766767673612No Hit
AAAGTACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAGTACTC6280.1401304016030204No Hit
GCTTAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT6130.13678333787046418No Hit
ATACCACTGCTTCGCAAAAAAAAAAAAAAGTACTCTGCGTTGATACCACTG6000.13388254930224877No Hit
GGTATCAACGCAGAGTACTTTTTGCAAGCAGTGGTATCAACGCAGAGTACT5940.1325437238092263No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAGTA5820.12986607282318133No Hit
TGATACCACTGCTTCGCAAAAAAAAAAAAGTACTCTGCGTTGATACCACTG5770.12875038491232924No Hit
CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTGCGAAGCAGTGGT5640.12584959634411386No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTGCGAAGCAGTGGTA5600.1249570460154322No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTGCGAAGCAGTGGTATCAAC5590.12473390843326178No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTGCGAAGCAGTGGTATC5590.12473390843326178No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTCGAAGCAGTGGTATCAA5560.12406449568675053No Hit
TGATACCACTGCTTCGCAAAAAAAAAAAAAAAGTACTCTGCGTTGATACCA5460.1218331198650464No Hit
ACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAGTACTCTG5460.1218331198650464No Hit
ACGCAGAGTACTTTTTTTTTTTTGCGAAGCAGTGGTATCAACGCAGAGTAC5430.12116370711853515No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTGCGAAGCAGTGGTA5380.12004801920768307No Hit
GCGTTGATACCACTGCTTAAGCAGTGGTATCAACGCAGAGTACTTTTTTTT5380.12004801920768307No Hit
GATACCACTGCTTCGCAAAAAAAAAAAAAAAAGTACTCTGCGTTGATACCA5290.11803978096814935No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTCGAAGCAGTGGTATCAAC5270.1175935058038085No Hit
AAAGTACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAGTACT5270.1175935058038085No Hit
ACTCTGCGTTGATACCACTGCTTGCAAAAAGTACTCTGCGTTGATACCACT5260.1173703682216381No Hit
GCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAAGTACTCTGCG5240.11692409305729726No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTGCGAAGCAGTG5230.11670095547512685No Hit
TATCAACGCAGAGTACTTTTTGCAAGCAGTGGTATCAACGCAGAGTACTTT5200.11603154272861561No Hit
CCACTGCTTCGCAAAAAAAAAAAAAAAAGTACTCTGCGTTGATACCACTGC5170.11536212998210438No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAGT5060.1129076165782298No Hit
GTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAGTACTCTGCGTTGATAC5060.1129076165782298No Hit
GATACCACTGCTTCGCAAAAAAAAAAAAAAAAAGTACTCTGCGTTGATACC5040.11246134141388898No Hit
GTTGATACCACTGCTTCGCAAAAAAAAAAAAGTACTCTGCGTTGATACCAC5030.11223820383171856No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTGCGAAGCAGTGGTATCAACGCAGAG4980.11112251592086649No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAA4800.10710603944179903No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTGCGAAGCAGTGGTATCAACGCAG4760.10621348911311736No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTGCAAGCAGTGGTATCAACG4740.10576721394877654No Hit
TGATACCACTGCTTCGCAAAAAAAAAAAAAAAAGTACTCTGCGTTGATACC4720.10532093878443571No Hit
CCACTGCTTCGCAAAAAAAAAAAAGTACTCTGCGTTGATACCACTGCTTCG4720.10532093878443571No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTGCAAGCAGTGGTATCAAC4670.10420525087358364No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAGTACTC4650.1037589757092428No Hit
GCGTTGATACCACTGCTTGCAAAAAAAAAAAAAGTACTCTGCGTTGATACC4630.10331270054490196No Hit
GCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAGTACTCTGCGTTG4580.10219701263404991No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTGCGAAGCAGTGGTATCA4540.10130446230536824No Hit
GTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAGTACTCTGCGTTGATA4530.10108132472319784No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC1550.040.64425345
ATGCCGT1550.040.64425344
GTATGCC1600.039.37412342
TATGCCG1600.039.37412343
TCTCGTA1600.039.37412338
CGTATGC1600.039.37412341
TCGTATG1600.039.37412340
CTCGTAT1600.039.37412339
CGAGACG1550.039.19267323
GCTATCT1450.038.79223634
CGAATTG1450.038.79223628
AGACGAA1450.038.79223625
GAATTGC1450.038.79223629
GACGAAT1450.038.79223626
ACGAATT1450.038.79223627
AATTGCT1450.038.79223630
ATCTCGT1650.038.18096537
ACGAGAC1650.038.18096522
TATCTCG1650.038.18096536
CTATCTC1600.037.96790335
Produced by FastQC (version 0.11.2)