##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename H23NVBCXX l02n01 36h_18.34100000005ca6.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 67781 Sequences flagged as poor quality 0 Sequence length 51 %GC 27 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.42808456647143 38.0 38.0 38.0 32.0 38.0 2 36.32751065932931 38.0 38.0 38.0 32.0 38.0 3 36.320901137486906 38.0 38.0 38.0 32.0 38.0 4 36.33692332659595 38.0 38.0 38.0 32.0 38.0 5 36.390020802289726 38.0 38.0 38.0 32.0 38.0 6 38.31830453962025 40.0 38.0 40.0 38.0 40.0 7 38.23379708177808 40.0 38.0 40.0 38.0 40.0 8 38.22043050412358 40.0 38.0 40.0 38.0 40.0 9 38.15516147592983 40.0 38.0 40.0 38.0 40.0 10 38.14220799339048 40.0 38.0 40.0 38.0 40.0 11 38.07680618462401 40.0 38.0 40.0 32.0 40.0 12 37.981882828521265 40.0 38.0 40.0 32.0 40.0 13 37.87660258774583 40.0 38.0 40.0 32.0 40.0 14 37.79714079166728 40.0 38.0 40.0 32.0 40.0 15 37.85726088431861 40.0 38.0 40.0 32.0 40.0 16 37.860683672415576 40.0 38.0 40.0 32.0 40.0 17 38.00239005030908 40.0 38.0 40.0 32.0 40.0 18 38.0 40.0 38.0 40.0 32.0 40.0 19 38.077543854472495 40.0 38.0 40.0 32.0 40.0 20 38.07519806435432 40.0 38.0 40.0 32.0 40.0 21 38.12373674038447 40.0 38.0 40.0 38.0 40.0 22 38.10820141337543 40.0 38.0 40.0 32.0 40.0 23 38.07720452634219 40.0 38.0 40.0 32.0 40.0 24 38.041353771705936 40.0 38.0 40.0 32.0 40.0 25 38.01423702807571 40.0 38.0 40.0 32.0 40.0 26 37.93830129387291 40.0 38.0 40.0 32.0 40.0 27 37.89188710700639 40.0 38.0 40.0 32.0 40.0 28 37.86406220032163 40.0 38.0 40.0 32.0 40.0 29 37.82529027308538 40.0 38.0 40.0 32.0 40.0 30 37.78162021805521 40.0 38.0 40.0 32.0 40.0 31 37.7702010888007 40.0 38.0 40.0 32.0 40.0 32 37.698632360100916 40.0 38.0 40.0 32.0 40.0 33 37.63774509080716 40.0 38.0 40.0 32.0 40.0 34 37.59884038299818 40.0 38.0 40.0 32.0 40.0 35 37.59653885307092 40.0 38.0 40.0 32.0 40.0 36 37.51732786474086 40.0 38.0 40.0 32.0 40.0 37 37.455348844071345 40.0 38.0 40.0 32.0 40.0 38 37.36291881205648 40.0 38.0 40.0 32.0 40.0 39 37.272687036190085 40.0 38.0 40.0 32.0 40.0 40 37.18086189345097 40.0 38.0 40.0 32.0 40.0 41 37.11692067098449 40.0 38.0 40.0 32.0 40.0 42 37.04349301426654 40.0 38.0 40.0 32.0 40.0 43 36.91817766040631 40.0 38.0 40.0 32.0 40.0 44 36.769079830631 40.0 38.0 40.0 27.0 40.0 45 36.650964134491964 40.0 38.0 40.0 27.0 40.0 46 36.52080966642569 40.0 38.0 40.0 27.0 40.0 47 36.38491612693823 40.0 38.0 40.0 27.0 40.0 48 36.27031173927797 40.0 38.0 40.0 27.0 40.0 49 36.15572210501468 40.0 38.0 40.0 27.0 40.0 50 36.05494165031499 40.0 38.0 40.0 27.0 40.0 51 35.91711541582449 40.0 38.0 40.0 27.0 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2201 1 -0.5743893284645267 2201 2 -0.36343291317533044 2201 3 0.3322584570177938 2201 4 -0.03526143061790776 2201 5 -0.4247925864708364 2201 6 -0.053186638471842684 2201 7 0.009691109662732345 2201 8 0.4987634256266986 2201 9 0.2773796528848891 2201 10 0.09423088743337615 2201 11 0.22734211167382057 2201 12 1.0541977512500011 2201 13 0.38036924705634334 2201 14 0.616962097752392 2201 15 -0.11535639135271936 2201 16 0.19878462674201103 2201 17 0.6429685414067237 2201 18 0.13218080076703131 2201 19 0.01760188180851685 2201 20 0.0456429371765239 2201 21 0.7016684497257444 2201 22 0.20306165949941857 2201 23 0.15248901943866855 2201 24 -0.03178634000442315 2201 25 0.43902612789145934 2201 26 -0.2444757599196734 2201 27 0.7103648155337936 2201 28 -0.24944328848584263 2201 29 0.2506178090880695 2201 30 0.6965008406884365 2201 31 -0.33745853818229676 2201 32 0.17246943228593636 2201 33 -0.1298994384081098 2201 34 -0.1866659439113647 2201 35 0.041589446361975035 2201 36 0.1765632551391576 2201 37 -0.6114651142652718 2201 38 0.25476637664993973 2201 39 0.06879775120709297 2201 40 -0.3173846327846661 2201 41 -0.2391089599342351 2201 42 -1.2709999335015496 2201 43 -0.6815592961451671 2201 44 0.08927940422167069 2201 45 -0.1853223507737809 2201 46 -0.4991030053217358 2201 47 -0.07760433895580832 2201 48 0.14870131824044108 2201 49 -0.44137795426824766 2201 50 -0.1279849136422726 2201 51 -0.13086031489190475 1101 1 0.34952371501373136 1101 2 0.07134969552031833 1101 3 -0.4286111081995969 1101 4 -0.3396092567048683 1101 5 -0.21827084734040625 1101 6 -0.08579533412401474 1101 7 0.16186502270620906 1101 8 0.4552851647571359 1101 9 0.0925970441892332 1101 10 0.6485787135203296 1101 11 0.7925595029781718 1101 12 0.3476760121195639 1101 13 -0.06528292685669612 1101 14 0.616962097752392 1101 15 0.05855665212554584 1101 16 0.6226976702202691 1101 17 0.5451424544502004 1101 18 0.751746018158336 1101 19 0.4741236209389541 1101 20 -0.019574454127820218 1101 21 0.4190597540735723 1101 22 0.7030616594994186 1101 23 0.4350977150908406 1101 24 0.1638658339086163 1101 25 0.3955478670218966 1101 26 0.4403068487759825 1101 27 0.047321337272919095 1101 28 0.33751323325328997 1101 29 0.30496563517502295 1101 30 0.19650084068843654 1101 31 0.47775885312204736 1101 32 -0.12100882858362638 1101 33 0.3483614311571017 1101 34 -0.034492030867880885 1101 35 0.17202422897067038 1101 36 -0.8777845709477958 1101 37 -0.2310303316565765 1101 38 -0.3647988407413649 1101 39 -0.4746805096624698 1101 40 -0.29564550234987763 1101 41 0.1087171270222882 1101 42 0.17465224041149696 1101 43 -0.5619940787538624 1101 44 -1.0737640740392038 1101 45 -0.3374962638172576 1101 46 -0.2599725705391265 1101 47 -1.2732565128688478 1101 48 -0.30782042088999617 1101 49 -0.5826823020943337 1101 50 -0.35624578320749123 1101 51 -0.03303422793538857 2202 1 0.716313724264424 2202 2 -1.044283977005115 2202 3 0.18147214804461953 2202 4 -0.06416984874926612 2202 5 -0.17918666602680844 2202 6 -0.2497638817650909 2202 7 0.3771749209485762 2202 8 -0.22834110721326084 2202 9 0.6430133254103225 2202 10 0.27230674312255587 2202 11 0.08580649650268413 2202 12 -0.4985312034215994 2202 13 0.15303344687133347 2202 14 0.05683258803916402 2202 15 -0.4162352072639095 2202 16 -0.28017004485835884 2202 17 0.2895920381689834 2202 18 -0.17702363956599498 2202 19 0.623059791151718 2202 20 -0.2873820396967375 2202 21 0.03631229801436575 2202 22 0.23081374090551776 2202 23 0.17099040704273705 2202 24 -0.006809466738928904 2202 25 0.007481262026523439 2202 26 -0.09854606519256492 2202 27 -0.07594415763919926 2202 28 -0.7825145188281155 2202 29 -0.05696775982960389 2202 30 -0.8543779752227536 2202 31 -0.30161209969941183 2202 32 -0.6103705307112861 2202 33 -0.4374850073257832 2202 34 -0.16099526861071212 2202 35 -0.19129677010426605 2202 36 -0.6220028873215284 2202 37 -0.5305215434974642 2202 38 0.7573103174455014 2202 39 0.06486620634122886 2202 40 -0.5979119223313774 2202 41 -0.3822634465207315 2202 42 -0.2952830047318926 2202 43 -1.077720258217326 2202 44 -0.6942543608107101 2202 45 0.624807158939447 2202 46 -1.0293990275233966 2202 47 -0.6469845424710741 2202 48 -0.30573901478453536 2202 49 0.14002815118966794 2202 50 0.026039138161614517 2202 51 0.035189638854625116 1102 1 0.27343675849198945 1102 2 0.6800453476942323 1102 3 0.2996497613656146 1102 4 0.09517335199078758 1102 5 0.44477263092046826 1102 6 0.2185524919629387 1102 7 0.2053432835757718 1102 8 0.41180690388757313 1102 9 -0.059576868854243514 1102 10 0.26814393091163424 1102 11 0.04255950297817179 1102 12 0.05419775125000115 1102 13 0.4129779427085225 1102 14 -0.18738572833456857 1102 15 0.13464360864728064 1102 16 0.42704549630722966 1102 17 0.03427288923280969 1102 18 0.0669634094626872 1102 19 0.19151492528678205 1102 20 0.24129511108957047 1102 21 0.12558149320400958 1102 22 0.5400181812385441 1102 23 0.7177064107430198 1102 24 0.02256148608253028 1102 25 0.16728699745667797 1102 26 0.2446546748629359 1102 27 -0.061374314900994875 1102 28 0.05490453760111791 1102 29 -0.10807784308584445 1102 30 -0.0208904636593914 1102 31 0.39080233138291476 1102 32 0.16159986706854568 1102 33 0.5657527355049297 1102 34 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28 0.8397732418628507 2204 29 0.41290795114574763 2204 30 0.5230182750405703 2204 31 0.23841317942424212 2204 32 0.6562188941886475 2204 33 1.1462520897881845 2204 34 0.9654003496745176 2204 35 0.734551133835069 2204 36 1.2776179258236198 2204 37 1.3265460781583158 2204 38 0.755519712853129 2204 39 0.9632230633035235 2204 40 1.4532783030741427 2204 41 0.9930262101045102 2204 42 1.2956165107515716 2204 43 1.139469545869467 2204 44 1.174083536808837 2204 45 1.4600715364410277 2204 46 1.6363898917940674 2204 47 1.4140013433515506 2204 48 1.0870353690368262 2204 49 0.6371842497782438 2204 50 0.7448088874769709 2204 51 0.9655667191158415 2205 1 0.5603522630038853 2205 2 0.32524633210768883 2205 3 -0.08488674396662077 2205 4 0.33245801154779997 2205 5 0.4960441649647649 2205 6 0.37749424750026606 2205 7 0.17765665519185347 2205 8 0.3720201934691971 2205 9 0.018766035165441508 2205 10 -0.20601521593003724 2205 11 -0.15124689570927785 2205 12 -0.2542517975604994 2205 13 -0.4270548710732669 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30 -1.515699029525706 1213 31 -0.8914818996358989 1213 32 -0.9642924106731812 1213 33 -0.6162719238072043 1213 34 -0.9129800256764469 1213 35 -1.5160030260585344 1213 36 -0.9008215599159968 1213 37 -1.0459232583275693 1213 38 -0.6405937790930807 1213 39 -0.5786714246527325 1213 40 -0.9126150026743431 1213 41 -0.5643847548725915 1213 42 -1.203349057447042 1213 43 -0.5473931702528887 1213 44 -0.5502403881209688 1213 45 -0.6017727076848729 1213 46 0.009819772095525536 1213 47 -1.0175783817851425 1213 48 -0.39656149811257535 1213 49 -0.18991786341814532 1213 50 0.18577238681197628 1213 51 -0.6548706977601739 >>END_MODULE >>Per sequence quality scores pass #Quality Count 15 1.0 16 5.0 17 5.0 18 5.0 19 6.0 20 22.0 21 19.0 22 51.0 23 101.0 24 158.0 25 235.0 26 358.0 27 545.0 28 730.0 29 866.0 30 1138.0 31 1446.0 32 1775.0 33 2163.0 34 2531.0 35 3232.0 36 4110.0 37 5546.0 38 9641.0 39 33092.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.14433293978749 25.83972845336482 14.239964580873671 17.775974025974026 2 10.89849513130717 28.310711124225435 36.08439067571555 24.706403068751843 3 20.547055959634704 34.25591242383559 31.25802215960225 13.939009456927456 4 10.774405806937047 24.12180404538145 36.541213614434724 28.56257653324678 5 11.551909827237722 38.13015446806627 32.880895826263995 17.43703987843201 6 28.59060798748912 41.42016199229872 13.049379619657426 16.93985040055473 7 14.46718106844101 44.578864283501275 20.315427627211164 20.63852702084655 8 21.09293164751184 37.20364113837211 21.956005370236497 19.747421843879554 9 20.16936899721161 26.44251338870775 22.299759519629394 31.088358094451245 10 13.95671353329104 35.48339505171066 26.985438397190954 23.57445301780735 11 23.075788200233106 31.44981632020773 21.64028267508594 23.83411280447323 12 19.90528319145483 36.60760390079816 28.94026349566988 14.546849412077131 13 24.84767117628834 29.63219781354657 27.336569245068677 18.183561765096414 14 18.419616116610847 30.71067113202815 32.18158480990248 18.68812794145852 15 21.162272613269206 33.38693734232307 29.6351484929405 15.815641551467225 16 13.031867807612866 39.182649749188556 28.723812334021837 19.06167010917675 17 12.684970714507015 37.88377273867308 33.19661852141456 16.23463802540535 18 13.051047506639128 31.454706403068748 41.52847447624668 13.96577161404544 19 13.34444755905047 36.44974255322288 38.40751833109574 11.798291556630915 20 13.114294566323897 32.045853557781676 39.93597025715171 14.903881618742714 21 9.139729422699576 37.811481093521785 39.04191440079078 14.006875082987857 22 9.74461869845532 39.13928682079049 39.64237765745563 11.473716823298565 23 9.091043212699725 35.92894764019415 40.810846697452085 14.169162449654033 24 11.64485622814653 37.82328381109751 41.22836783169325 9.303492129062716 25 11.053245009663474 37.73181274988566 40.03776869624231 11.177173544208555 26 10.47343650875614 37.48543102049246 41.699001195025154 10.342131275726237 27 10.792109883300629 38.9563446983668 41.024770953512046 9.226774464820526 28 8.905150410882106 37.450022867765306 41.05427774745135 12.590548973901242 29 9.87739927118219 37.64181702837078 42.86894557471858 9.611838125728449 30 9.820005901445853 38.62643847742697 41.596341103570374 9.957214517556801 31 12.05647600359983 37.973768460187955 40.2546436316962 9.715111904516014 32 9.821336362697512 40.0805535474543 40.2841504256355 9.813959664212685 33 8.915477788760862 39.74565143624319 41.85538720290347 9.483483572092474 34 9.153007479972263 39.79433764624305 40.06875082987858 10.98390404390611 35 9.144155441790472 40.04219471533321 39.83417181806111 10.979478024815213 36 9.006948849972707 40.23546421563565 41.51753441229843 9.240052522093212 37 8.96859001785161 41.51310839320754 39.4358300998805 10.082471489060357 38 9.383160472698838 40.046620734424096 40.82264941502781 9.74756937784925 39 9.499712308759092 39.74565143624319 39.331080981395964 11.423555273601748 40 9.726914622091737 39.874005989879166 40.72822767442204 9.670851713607059 41 9.95411693542438 39.50222038624393 38.92831324412446 11.615349434207225 42 11.360115666632243 40.163172570484356 38.7143889880645 9.762322774818902 43 9.36398105663829 40.38299818533218 40.10710966199967 10.145911096029861 44 9.597084728758796 41.94833360381228 38.18179135746006 10.272790309968869 45 9.918708782697216 40.220710818666 39.22190584382054 10.638674554816246 46 11.451586727844086 39.93597025715171 37.60493353594665 11.007509479057553 47 10.392446149306581 40.079669519032166 37.06107996459133 12.466804367069932 48 10.03230993936354 40.23251353624172 36.82300349655508 12.91217302783966 49 12.052049984508933 39.949248314424395 36.93807999291837 11.0606217081483 50 9.999852466030305 40.6279045750284 38.28801581564155 11.084227143299746 51 10.09472100091475 40.55740800849833 36.38791348225088 12.959957508336037 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3431.0 1 1963.5 2 496.0 3 762.0 4 1028.0 5 1092.0 6 1156.0 7 1852.5 8 2549.0 9 1850.0 10 1151.0 11 1856.5 12 2562.0 13 4000.0 14 5438.0 15 5675.5 16 5913.0 17 4477.5 18 3042.0 19 2470.0 20 1898.0 21 2678.5 22 3459.0 23 2369.0 24 1279.0 25 1441.5 26 2377.0 27 3150.0 28 2257.5 29 1365.0 30 1493.5 31 1622.0 32 1895.5 33 2169.0 34 2301.5 35 2434.0 36 2442.0 37 2450.0 38 2317.0 39 2184.0 40 2109.0 41 2034.0 42 1926.0 43 1818.0 44 1816.0 45 1814.0 46 1728.5 47 1643.0 48 1779.5 49 1916.0 50 2343.5 51 2771.0 52 2084.0 53 1397.0 54 1290.5 55 1184.0 56 1053.5 57 923.0 58 816.0 59 709.0 60 602.5 61 496.0 62 401.0 63 306.0 64 243.5 65 181.0 66 137.5 67 94.0 68 75.0 69 56.0 70 43.0 71 30.0 72 21.0 73 12.0 74 11.0 75 6.5 76 3.0 77 3.0 78 3.0 79 2.0 80 1.0 81 0.5 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03098213363626975 2 0.0014753396969652264 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0014753396969652264 17 0.0 18 0.0014753396969652264 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0014753396969652264 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0014753396969652264 48 0.0 49 0.0 50 0.0 51 0.004426019090895679 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 67781.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.49296686123034 #Duplication Level Percentage of deduplicated Percentage of total 1 91.32280388845267 44.285137026373725 2 6.0891074909463505 5.905577755458616 3 1.0639577010233083 1.5478339661242235 4 0.35705118287887333 0.6925788471643319 5 0.20512744208347386 0.4973619125641422 6 0.13341153586504573 0.38817127125657036 7 0.07746143592179254 0.2629434391625162 8 0.08809921645130533 0.3417753907098807 9 0.03405464106812908 0.14862695227090894 >10 0.40673589060049575 3.994542417132372 >50 0.07913603625186388 2.6405232823907205 >100 0.09739815384286983 10.083872828590255 >500 0.02739323076829799 9.701594862750733 >1k 0.018262153845531995 19.509460048050993 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3504 5.169590298166153 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3288 4.850916923621664 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2099 3.09673802393001 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1633 2.4092297251442143 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1615 2.3826736105988404 No Hit CTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTC 1079 1.591891533025479 No Hit GCTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 896 1.3219043684808427 No Hit GCGTTGATACCACTGCTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 839 1.2378100057538248 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 828 1.2215812690872072 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 825 1.2171552499963116 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 717 1.0578185627240673 No Hit GCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 717 1.0578185627240673 No Hit GCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 691 1.0194597306029713 No Hit CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 537 0.7922574172703265 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 523 0.7716026615128133 No Hit ATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 421 0.6211180124223602 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 359 0.5296469512105162 No Hit GATACCACTGCTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 351 0.5178442336347945 No Hit AAAAAGTACTCTGCGTTGATACCACTGCTTGGAAAAAAAAAAAAAAAAAAA 339 0.5001401572712117 No Hit GCGTTGATACCACTGCTTCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 311 0.4588306457561853 No Hit AAAAAGTACTCTGCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAA 290 0.4278485121199156 No Hit ATACCACTGCTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 284 0.4189964739381243 No Hit CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 280 0.4130951151502633 No Hit TGATACCACTGCTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 271 0.3998170578775763 No Hit GCTTCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 262 0.38653900060488927 No Hit GATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 258 0.38063764181702836 No Hit ACTCTGCGTTGATACCACTGCTTGGAAAAAAAAAAAAAAAAAAAAAAAAAA 247 0.36440890515041086 No Hit AAAGTACTCTGCGTTGATACCACTGCTTGGAAAAAAAAAAAAAAAAAAAAA 244 0.35998288605951523 No Hit CCACTGCTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 239 0.3526061875746891 No Hit AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 208 0.3068706569687671 No Hit AAAGTACTCTGCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAA 208 0.3068706569687671 No Hit ACTCTGCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAA 202 0.2980186187869757 No Hit GTTGATACCACTGCTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 197 0.2906419203021496 No Hit TGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 157 0.23162833242354053 No Hit TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 155 0.22867765302961007 No Hit GCGTTGATACCACTGCTTCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 148 0.2183502751508535 No Hit GTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 146 0.21539959575692302 No Hit CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 141 0.20802289727209688 No Hit GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT 140 0.20654755757513166 No Hit CTGCTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 133 0.1962201796963751 No Hit CCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 133 0.1962201796963751 No Hit AAAAAGTACTCTGCGTTGATACCACTGCTTCGAAAAAAAAAAAAAAAAAAA 132 0.19474483999940986 No Hit TACCACTGCTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 126 0.1858928018176185 No Hit GATACCACTGCTTCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 123 0.18146678272672284 No Hit TTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTCT 115 0.16966406515100102 RNA PCR Primer, Index 27 (95% over 21bp) AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT 110 0.1622873666661749 No Hit CCACTGCTTCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 102 0.1504846490904531 No Hit GCTTCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 97 0.14310795060562695 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 93 0.13720659181776604 No Hit CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 86 0.12687921393900947 No Hit AAAGTACTCTGCGTTGATACCACTGCTTCGAAAAAAAAAAAAAAAAAAAAA 86 0.12687921393900947 No Hit ACTCTGCGTTGATACCACTGCTTCGAAAAAAAAAAAAAAAAAAAAAAAAAA 85 0.12540387424204422 No Hit TGATACCACTGCTTCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 81 0.11950251545418333 No Hit TACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 79 0.11655183606025286 No Hit GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 76 0.1121258169693572 No Hit GCTTAGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 76 0.1121258169693572 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 73 0.10769979787846151 No Hit CTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 70 0.10327377878756583 No Hit ATACCACTGCTTCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 69 0.10179843909060061 No Hit TTGATACCACTGCTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 68 0.10032309939363539 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0 0.0 0.0 2 0.0 0.0 0.0 3 0.0 0.0 0.0 4 0.0 0.0 0.0 5 0.0 0.0 0.0 6 0.0 0.0 0.0 7 0.0 0.0 0.0 8 0.0 0.0 0.0 9 0.0 0.0 0.0 10 0.0 0.0 0.0 11 0.0 0.0 0.0 12 0.0 0.0 0.0 13 0.0 0.0 0.0 14 0.0 0.0 0.0 15 0.0 0.0 0.0 16 0.0 0.0 0.0029506793939304524 17 0.0 0.0 0.0029506793939304524 18 0.0 0.0 0.004426019090895679 19 0.0 0.0 0.005901358787860905 20 0.0 0.0 0.010327377878756584 21 0.0 0.0 0.02360543515144362 22 0.0 0.0 0.032457473333234975 23 0.0 0.0 0.048686209999852466 24 0.0 0.0 0.07966834363612221 25 0.0 0.0 0.08999572151487881 26 0.0 0.0 0.1180271757572181 27 0.0 0.0 0.12835455363597467 28 0.0 0.0 0.17113940484796625 29 0.0 0.0 0.21244891636299257 30 0.0 0.0 0.2419557103022971 31 0.0 0.0 0.3762116227261327 32 0.0 0.0 0.4721087030288724 33 0.0 0.0 0.5621044245437512 34 0.0 0.0 0.6948849972706216 35 0.0 0.0 0.7907820775733613 36 0.0 0.0 1.0002803145424235 37 0.0 0.0 1.1787964178752157 38 0.0 0.0 1.3941960136321387 39 0.0 0.0 1.6140216284799576 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGCCGTC 105 0.0 42.857143 45 TATGCCG 105 0.0 42.857143 43 CACGAGA 105 0.0 42.857143 21 ACGAGAC 105 0.0 42.857143 22 CTCGTAT 105 0.0 42.857143 39 CGAGACT 100 0.0 42.75 23 TATCTCG 95 0.0 42.63158 36 CTAGATC 95 0.0 42.63158 28 ACTAGAT 95 0.0 42.63158 27 TAGATCC 95 0.0 42.63158 29 GACTAGA 95 0.0 42.63158 26 CCTATCT 90 0.0 42.5 34 AGCCCAC 110 0.0 40.90909 17 GTATGCC 110 0.0 40.90909 42 ATCTCGT 110 0.0 40.90909 37 CCACGAG 110 0.0 40.90909 20 TCTCGTA 110 0.0 40.90909 38 ATGCCGT 110 0.0 40.90909 44 CGAGCCC 110 0.0 40.90909 15 TCCTATC 100 0.0 40.5 33 >>END_MODULE