FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 36h_17.34100000005c8c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 36h_17.34100000005c8c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences76346
Sequences flagged as poor quality0
Sequence length51
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA29533.867917114190658No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16822.2031278652450683No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10411.3635291960286067No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7620.9980876535771357No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7310.9574830377491945No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTC4830.6326461111256647No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4160.5448877478846305No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3160.4139051161815943No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2780.36413171613444056No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1710.22398030021219187No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1600.20957221072485788No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1390.18206585806722028No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1240.16241846331176488No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA930.12181384748382364No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTCGT406.672053E-945.037
CGTATGC406.672053E-945.041
CTCGTAT406.672053E-945.039
TCTCGTA406.672053E-945.038
TCGTATG406.672053E-945.040
CGAGACT406.672053E-945.023
ACGAGAC406.672053E-945.022
TGCCGTC406.672053E-945.045
CTATCTC451.888111E-839.99999635
CACGAGA451.888111E-839.99999621
TATGCCG451.888111E-839.99999643
TATCTCG451.888111E-839.99999636
ATGCCGT451.888111E-839.99999644
CCACGAG451.888111E-839.99999620
GATCCTA403.401983E-739.37531
TCCTATC403.401983E-739.37533
GAGACTA403.401983E-739.37524
GTGTAGC301.1299635E-437.4999961
CGTGGAA250.002094829236.09
CATGCTG250.002094829236.09