FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 36h_16.34100000005c7f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 36h_16.34100000005c7f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42561
Sequences flagged as poor quality0
Sequence length51
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTTGCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT17844.191630835741641No Hit
GCGTTGATACCACTGCTTGCAAGCAGTGGTATCAACGCAGAGTACTTTTTT16963.9848687765794977No Hit
CTTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTC16083.7781067174173537TruSeq Adapter, Index 12 (95% over 21bp)
ATACCACTGCTTGCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTT7761.8232654307934495No Hit
GATACCACTGCTTGCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTT7321.7198844012123775No Hit
CTGCTTGCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTT6061.4238387255938536No Hit
TGATACCACTGCTTGCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTT5151.210027959869364No Hit
ACTCTGCGTTGATACCACTGCTTGCAAGCAGTGGTATCAACGCAGAGTACT4261.0009163318531051No Hit
CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3420.8035525481074222No Hit
TGCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT2980.7001715185263504No Hit
CTTGCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT2870.6743262611310825No Hit
CCACTGCTTGCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTT2850.6696271234228519No Hit
GTTGATACCACTGCTTGCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTT2820.6625784168605061No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTGCAAGCAGTGGTATCAACGCC1870.4393693757195554No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1780.41822325603251803No Hit
TTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTCT1730.4064754117619417TruSeq Adapter, Index 12 (95% over 22bp)
AAAGTACTCTGCGTTGATACCACTGCTTGCAAGCAGTGGTATCAACGCCTG1620.38063015436667375No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTGCAAGCAGTGGTATCAACGCA1430.3359883461384836No Hit
GCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAGTACTCTGCGTTGATAC1400.32893963957613775No Hit
TACCACTGCTTGCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTT1390.3265900707220225No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1350.3171917953055614No Hit
ACTCTGCGTTGATACCACTGCTTGCAAGCAGTGGTATCAACGCCTGTCTCT1310.30779351988910036No Hit
AAAGTACTCTGCGTTGATACCACTGCTTGCAAGCAGTGGTATCAACGCAGA1200.28194826249383237No Hit
CTGCGTTGATACCACTGCTTGCAAGCAGTGGTATCAACGCAGAGTACTTTT1190.2795986936397171No Hit
TTGATACCACTGCTTGCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTT1190.2795986936397171No Hit
GCGTTGATACCACTGCTTGCAAGCAGTGGTATCTGTCTCTTATACACATCT1180.2772491247856018No Hit
GCGTTGATACCACTGCTTGCAAGCAGTGGTATCCTGTCTCTTATACACATC1070.2514038673903339No Hit
GCGTTGATACCACTGCTTCGCAAAAAAAAAAAAGTACTCTGCGTTGATACC1050.24670472968210336No Hit
GCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAGTACTCTGCGTTGATA980.23025774770329643No Hit
GCGTTGATACCACTGCTTAAGCAGTGGTATCAACGCAGAGTACTTTTTTTT950.22320904114095064No Hit
CTCTGCGTTGATACCACTGCTTGCAAGCAGTGGTATCAACGCAGAGTACTT930.21850990343272012No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTGCGAAGCAGTGGTATCAACGC900.2114611968703743No Hit
GCTTGCAAGCAGTGGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACAC860.2020629214539132No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTGCAAGCAGTGGTATCTGTCTC840.1973637837456827No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT840.1973637837456827No Hit
GCGTTGATACCACTGCTTGCAAAAAAAAAAAAAGTACTCTGCGTTGATACC790.18561593947510632No Hit
ACTCTGCGTTGATACCACTGCTTGCAAGCAGTGGTATCTGTCTCTTATACA760.1785672329127605No Hit
GCTTGCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTG740.17386809520452995No Hit
CACTGCTTGCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTT740.17386809520452995No Hit
ATACCACTGCTTGCAAGCAGTGGTATCAACGCAGAGTACTTTTTCTGTCTC730.1715185263504147No Hit
GCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAGTACTCTGCGTTGAT710.16681938864218415No Hit
CGTTGATACCACTGCTTGCAAGCAGTGGTATCAACGCAGAGTACTTTTTTT710.16681938864218415No Hit
AAAGTACTCTGCGTTGATACCACTGCTTGCAAGCAGTGGTATCTGTCTCTT710.16681938864218415No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTGCAAGCCTGTCTCTTATACAC680.15977068207983836No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTGCAAGCAGTGGTATCCTGTCT670.15742111322572308No Hit
GCTTGCAAGCAGTGGTATCTGTCTCTTATACACATCTCCGAGCCCACGAGA660.1550715443716078No Hit
GCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAGTACTCTGCGTTGATAC660.1550715443716078No Hit
GCGTTGATACCACTGCTTGCAAGCAGTGGTATCACTGTCTCTTATACACAT640.15037240666337726No Hit
GCTTAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT640.15037240666337726No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT640.15037240666337726No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTGCGAAGCAGTGGTATCAACGCA630.148022837809262No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT600.14097413124691618No Hit
GATACCACTGCTTGCAAGCAGTGGTATCAACGCAGAGTACTTTTTCTGTCT590.1386245623928009No Hit
AAAGTACTCTGCGTTGATACCACTGCTTGCAAGCAGTGGTATCCTGTCTCT590.1386245623928009No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTGCGAAGCAGTGGTATCAACG580.13627499353868563No Hit
ATACCACTGCTTGCAAGCAGTGGTATCAACGCCTGTCTCTTATACACATCT580.13627499353868563No Hit
ACCACTGCTTGCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTT560.1315758558304551No Hit
ACTCTGCGTTGATACCACTGCTTGCAAGCAGTGGTATCCTGTCTCTTATAC560.1315758558304551No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTGCGAAGCAGTGGTAT550.12922628697633984No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTGCGAAGCAGTGGTATCA550.12922628697633984No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTGCGAAGCAGTGGTATCAA550.12922628697633984No Hit
TTTTTGCAGAGCGGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA530.1245271492681093No Hit
GTACTCTGCGTTGATACCACTGCTTGCAAGCAGTGGTATCAACGCAGAGTA520.12217758041399403No Hit
TACCACTGCTTCGCAAAAAAAAAAAAAGTACTCTGCGTTGATACCACTGCT510.11982801155987877No Hit
CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTGCGAAGCAGTGGTATC500.1174784427057635No Hit
GATACCACTGCTTGCAAGCAGTGGTATCAACGCAGAGTACTTTCTGTCTCT490.11512887385164822No Hit
CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTGCAAGCAGTGGTATCA480.11277930499753297No Hit
GCTTGCAAGCAGTGGTATCACTGTCTCTTATACACATCTCCGAGCCCACGA480.11277930499753297No Hit
AAAGTACTCTGCGTTGATACCACTGCTTGCAAGCAGTGGTATCACTGTCTC480.11277930499753297No Hit
AAAAAAGTACTCTGCGTTGATACCACTGCTTGCAAGCAGTGGTATCAACGC460.10808016728930242No Hit
ATACCACTGCTTGCAAGCAGTGGTATCAACGCAGAGTCTGTCTCTTATACA460.10808016728930242No Hit
AAAAAAAAGTACTCTGCGTTGATACCACTGCTTGCAAGCAGTGGTATCAAC450.10573059843518715No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTGCGAAGCAGTGGTATCAAC440.10338102958107188No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTGCAAGCAGTGGTATCAACGC440.10338102958107188No Hit
GCTTGCAAGCAGTGGTATCAACGCAGAGTACTTTCTGTCTCTTATACACAT430.1010314607269566No Hit
CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTGCGAAGCAGTGGTAT430.1010314607269566No Hit
ATACCACTGCTTGCAAGCAGTGGTATCAACGCAGAGTACTTTCTGTCTCTT430.1010314607269566No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT430.1010314607269566No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTGCAAGCAGTGGTATCAAC430.1010314607269566No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT430.1010314607269566No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCTCG1850.042.5675736
TTGCAAT1800.042.532
TGCAATC1800.042.533
ACTTGCA1800.042.530
CAATCTC1800.042.535
GCAATCT1800.042.534
TACTTGC1800.042.529
CTACTTG1800.042.528
GTATGCC2050.041.70731742
TGCCGTC2050.041.70731745
TATGCCG2050.041.70731743
ATGCCGT2050.041.70731744
CGTATGC2050.041.70731741
ATCTCGT2000.041.62500437
TCTCGTA2000.041.62500438
TCGTATG2000.041.62500440
CTCGTAT2000.041.62500439
AGACTAC1900.041.44737225
GAGACTA1900.041.44737224
GACTACT1900.041.44737226
Produced by FastQC (version 0.11.2)