FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 36h_15.34100000005c62.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 36h_15.34100000005c62.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences202491
Sequences flagged as poor quality0
Sequence length51
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTC18030.8904099441456658TruSeq Adapter, Index 12 (95% over 21bp)
AAAAAGTACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAA13840.6834871673308938No Hit
GCGTTGATACCACTGCTTGCGCAAGCAGTGGTATCAACGCAGAGTACTTTT9570.47261359764137667No Hit
GCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAGTACTCTGCGTTGA8340.41187015719217146No Hit
AAAGTACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAA7880.38915309816238747No Hit
GCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAGTACTCTGCGTTGAT7830.3866838526156718No Hit
GCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAGTACTCTGCGTTGATA7440.3674237373512897No Hit
GCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAGTACTCTGCGTTG6600.3259404121664667No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTGGCAAAAAAAAAAAAAAAAAA6420.3170511281982903No Hit
GCGTTGATACCACTGCTTGGCAAAAAAAAAAAAAAGTACTCTGCGTTGATA6160.3042110513553689No Hit
GCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5290.26124617884251644No Hit
GCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAGTACTCTGCGTTGATAC5170.25531998953039886No Hit
GCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAGTACTCTGCGTT5140.2538384422023695No Hit
CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5130.25334459309302637No Hit
GCGTTGATACCACTGCTTGGCAAAAAAAAAAAAAAAGTACTCTGCGTTGAT4950.24445530912485No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTGCGAAGCAGTGGTATCAACG4620.22815828851652664No Hit
ATACCACTGCTTGCGCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTT4550.22470134475112472No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTGCGAAGCAGTGGTATCAAC4340.214330513454919No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTGCGAAGCAGTGGTATCAA4280.2113674187988602No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4260.21037972058017396No Hit
AAAGTACTCTGCGTTGATACCACTGCTTGGCAAAAAAAAAAAAAAAAAAAA4250.2098858714708308No Hit
GCGTTGATACCACTGCTTGGCAAAAAAAAAAAAAGTACTCTGCGTTGATAC4210.20791047503345828No Hit
GCTTGCGCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTT4120.2034658330493701No Hit
GATACCACTGCTTGCGCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTT4040.19951504017462507No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTGCGCAAGCAGTGGTATCAACG4010.19803349284659566No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3870.1911196053157918No Hit
GCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAGTACTCTGCGT3860.19062575620644867No Hit
ACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAAAAAAA3800.18766266155038988No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTGCGAAGCAGTGGTATCAACGC3640.17976107580089978No Hit
CTGCTTGCGCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTT3490.17235333916075282No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTGCGAAGCAGTGGTATC3480.17185949005140969No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTGCGAAGCAGTGGTATCA3430.16939024450469403No Hit
ACTCTGCGTTGATACCACTGCTTGCGCAAGCAGTGGTATCAACGCAGAGTA3410.1684025462860078No Hit
GCGTTGATACCACTGCTTCGCAAAAAAAAAAAAGTACTCTGCGTTGATACC3290.16247635697389018No Hit
GCGTTGATACCACTGCTTGGCAAAAAAAAAAAAAAAAGTACTCTGCGTTGA3240.16000711142717455No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTGCGAAGCAGTGGTAT3200.158031714989802No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT3170.15655016766177263No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTGCCAAGCAGTGGTATCAACG3150.15556246944308635No Hit
AAAAAAGTACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAA2980.14716703458425312No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTGCGAAGCAGTGGTATCA2960.14617933636556685No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2910.1437100908188512No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAGTA2900.14321624170950809No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTGCCAAGCAGTGGTATCA2750.13580850506936112No Hit
GCGTTGATACCACTGCTTGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2750.13580850506936112No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTGCCAAGCAGTGGTATCAAC2740.13531465596001796No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAGT2690.1328454104133023No Hit
TGATACCACTGCTTGCGCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTT2690.1328454104133023No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAG2670.13185771219461606No Hit
CGCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT2650.1308700139759298No Hit
GCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAAGTACTCTGCG2620.1293884666479004No Hit
AAAAAAAAGTACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAA2490.12296842822643969No Hit
GCGTTGATACCACTGCTTGGCAAAAAAAAAAAAAAAAAGTACTCTGCGTTG2440.12049918267972404No Hit
GATACCACTGCTTCGCAAAAAAAAAAAAAAAGTACTCTGCGTTGATACCAC2440.12049918267972404No Hit
ACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAGTACTCTGCG2420.11951148446103776No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTGCGAAGCAGTGGTATC2410.11901763535169464No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTGCGAAGCAGTGGTATCAA2400.11852378624235152No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTGCCAAGCAGTGGTATCAACGC2380.11753608802366525No Hit
CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTGCGAAGCAGTGGTA2360.11654838980497899No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTGCCAAGCAGTGGTATCAA2350.11605454069563587No Hit
GCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAGTACTCTGCGTTGATA2350.11605454069563587No Hit
GCGTTGATACCACTGCTTGCAAAAAAAAAAAAAGTACTCTGCGTTGATACC2290.11309144603957708No Hit
GATACCACTGCTTCGCAAAAAAAAAAAAAAAAAGTACTCTGCGTTGATACC2280.11259759693023394No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTGCGAAGCAGTGGTA2280.11259759693023394No Hit
GATACCACTGCTTGGCAAAAAAAAAAAAAAGTACTCTGCGTTGATACCACT2270.1121037478208908No Hit
ACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAGTACTCTGC2260.11160989871154767No Hit
TTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTCT2220.10963450227417515TruSeq Adapter, Index 12 (95% over 22bp)
GCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAGTACTCTGCGTTGATAC2220.10963450227417515No Hit
GATACCACTGCTTCGCAAAAAAAAAAAAAAGTACTCTGCGTTGATACCACT2200.10864680405548888No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTGCCAAGCAGTGGTATC2190.10815295494614575No Hit
ACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAGTACTCTGCGT2170.1071652567274595No Hit
TGATACCACTGCTTCGCAAAAAAAAAAAAAAAAGTACTCTGCGTTGATACC2160.10667140761811636No Hit
TACCACTGCTTCGCAAAAAAAAAAAAAAAGTACTCTGCGTTGATACCACTG2120.10469601118074383No Hit
AAAGTACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAGTAC2120.10469601118074383No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC1950.039.22883245
GACTACT1800.037.50741226
ATGCCGT2050.037.3152344
AGACTAC1850.036.493725
TTGCAAT1850.036.48468432
AATCTCG1850.036.48468436
TGCAATC1850.036.48468433
CAATCTC1850.036.48468435
GCAATCT1850.036.48468434
GTATGCC2100.036.42677342
ATCTCGT2100.036.42677337
TCTCGTA2100.036.42677338
CGTATGC2100.036.42677341
TCGTATG2100.036.42677340
CTCGTAT2100.036.42677339
GCTTCTG250.002100979436.00711427
CTTCTGT250.002100979436.00711428
GAGACGA250.002100979436.00711424
TATCTCG250.002103529235.99822236
CGAGACG250.002103529235.99822223
Produced by FastQC (version 0.11.2)