FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 36h_14.34100000005c56.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 36h_14.34100000005c56.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences227690
Sequences flagged as poor quality0
Sequence length51
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTC24911.09403135842593TruSeq Adapter, Index 12 (95% over 21bp)
AAAAAGTACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAA21640.95041503799025No Hit
GCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAGTACTCTGCGTTGA14880.6535201370284158No Hit
GCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAGTACTCTGCGTTGAT14260.6262901313188984No Hit
GCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAGTACTCTGCGTTG13460.5911546400808116No Hit
GCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAGTACTCTGCGTTGATA11800.5182484957617813No Hit
GCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAGTACTCTGCGTT11110.4879441345689315No Hit
AAAGTACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAA10820.475207518995125No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTGCGAAGCAGTGGTATCAAC8580.3768281435284817No Hit
GCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAGTACTCTGCGTTGATAC8520.3741929816856252No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTGCGAAGCAGTGGTATCAA8430.3702402389213404No Hit
GCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAGTACTCTGCGT8190.3596995915499143No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTGCGAAGCAGTGGTATCAACG6970.3061179674118319No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTGCGAAGCAGTGGTATCA6360.2793271553427906No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTGCGAAGCAGTGGTATC6330.27800957442136237No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTGCGAAGCAGTGGTAT6210.27273925073564936No Hit
GCGTTGATACCACTGCTTCGCAAAAAAAAAAAAGTACTCTGCGTTGATACC5900.25912424788089067No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTGCGAAGCAGTGGTATCAACGC5890.2586850542404146No Hit
GCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAAGTACTCTGCG5730.2516579559927972No Hit
GCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5400.2371645658570864No Hit
AAAAAAAAGTACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAA5070.22267117572137554No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTGCGAAGCAGTGGTATCA5060.22223198208089945No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAG5010.22003601387851907No Hit
GCGTTGATACCACTGCTTGCAAAAAAAAAAAAAGTACTCTGCGTTGATACC4910.21564407747375816No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTGCGAAGCAGTGGTATC4650.20422504282137993No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAGTA4630.2033466555404278No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAGT4610.20246826825947561No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTGCGAAGCAGTGGTA4550.19983310641661908No Hit
GATACCACTGCTTCGCAAAAAAAAAAAAAAAAAGTACTCTGCGTTGATACC4530.19895471913566692No Hit
ACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAGTACTCTGC4370.19192762088804954No Hit
AAAAAAGTACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAA4290.18841407176424085No Hit
ACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAGTACTCTG4210.18490052264043216No Hit
ACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAAAAAAA4210.18490052264043216No Hit
GCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAGTACTCTGCGTTGATAC4180.1835829417190039No Hit
GATACCACTGCTTCGCAAAAAAAAAAAAAAAGTACTCTGCGTTGATACCAC4060.17831261803329088No Hit
CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTGCGAAGCAGTGGT4040.17743423075233872No Hit
ACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAGTACTCTGCGT4040.17743423075233872No Hit
ACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAGTACTCTGCG4040.17743423075233872No Hit
TACCACTGCTTCGCAAAAAAAAAAAAAAAGTACTCTGCGTTGATACCACTG3990.17523826254995828No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTGCGAAGCAGTGGT3940.17304229434757784No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTGCGAAGCAGTGGTATCAAC3830.1682111643023409No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTGCGAAGCAGTGGTATCAA3830.1682111643023409No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTGCGAAGCAGTGG3750.16469761517853224No Hit
GCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAAAGTACTCTGC3750.16469761517853224No Hit
AAAGTACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAGTAC3720.16338003425710396No Hit
TACCACTGCTTCGCAAAAAAAAAAAAAAAAGTACTCTGCGTTGATACCACT3710.16294084061662786No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAGTAC3710.16294084061662786No Hit
CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTGCGAAGCAGTGGTA3700.16250164697615177No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTGCGAAGCAGTGGTATCAA3660.16074487241424743No Hit
AAAGTACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAGTA3640.15986648513329527No Hit
GATACCACTGCTTCGCAAAAAAAAAAAAAAGTACTCTGCGTTGATACCACT3550.1559137423690105No Hit
TGATACCACTGCTTCGCAAAAAAAAAAAAAAAAGTACTCTGCGTTGATACC3540.15547454872853442No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTGCGAAGCAGTGGTATCAACG3540.15547454872853442No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAA3510.15415696780710617No Hit
GATACCACTGCTTCGCAAAAAAAAAAAAAAAAGTACTCTGCGTTGATACCA3460.15196099960472573No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTGCGAAGCAGTGGTATCAACGCA3460.15196099960472573No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTGCGAAGCAGTGGTA3440.15108261232377357No Hit
AAAGTACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAGTACT3440.15108261232377357No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTGCGAAGCAGTGGT3400.1493258377618692No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTGCAAGCAGTGGTATCAACGCA3300.14493390135710835No Hit
TGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAGTACTCTGCGTTGATAC3300.14493390135710835No Hit
GTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAGTACTCTGCGTTGATA3210.1409811585928236No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTGCGAAGCAGTG3200.1405419649523475No Hit
TGATACCACTGCTTCGCAAAAAAAAAAAAAAAGTACTCTGCGTTGATACCA3180.1396635776713953No Hit
TACCACTGCTTCGCAAAAAAAAAAAAAAGTACTCTGCGTTGATACCACTGC3170.13922438403091922No Hit
ACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAGTACTCT3160.13878519039044315No Hit
CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTGCGAAGCAGTGGTAT3120.1370284158285388No Hit
GTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAGTACTCTGCGTTGATAC3090.13571083490711056No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTGCGAAGCAGTGGTATCAAC3060.13439325398568228No Hit
CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTGCGAAGCAGTGG3010.13219728578330187No Hit
GCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAGTACTCTGCGTTGATA3010.13219728578330187No Hit
AAAAAAAGTACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAA3010.13219728578330187No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTGCGAAGCAGTG2990.13131889850234968No Hit
GATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAGTACTCTGCGTTGATAC2990.13131889850234968No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTGCGAAGCAGT2980.13087970486187359No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTGCAAGCAGTGGTATCAACGC2970.13044051122139752No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTGCGAAGCAGTGGTATCAACGC2960.13000131758092143No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTGCGAAGCAGTGGTATCA2960.13000131758092143No Hit
TACCACTGCTTCGCAAAAAAAAAAAAAAAAAGTACTCTGCGTTGATACCAC2950.12956212394044533No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTGCGAAGCAG2940.12912293029996924No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTGCGAAGCAGTGG2880.12648776845711274No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTGCAAGCAGTGGTATCAAC2870.12604857481663664No Hit
TTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTCT2860.12560938117616055TruSeq Adapter, Index 12 (95% over 22bp)
AAAGTACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAGT2860.12560938117616055No Hit
AAAGTACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAGTACTC2810.12341341297378014No Hit
TGATACCACTGCTTCGCAAAAAAAAAAAAAAGTACTCTGCGTTGATACCAC2810.12341341297378014No Hit
GCGTTGATACCACTGCTTGCAAAAAAAAAAAAGTACTCTGCGTTGATACCA2800.12297421933330406No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTGCGAAGCAGTGGTAT2800.12297421933330406No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTGCGAAGCAGTGGTATCAAC2760.1212174447713997No Hit
ACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAGTACTCTGCGTT2740.12033905749044753No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTGCGAAGCAGTGGTATCAA2700.1185822829285432No Hit
GTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAGTACTCTGCGTTGAT2690.1181430892880671No Hit
ACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAGTACTC2650.11638631472616276No Hit
CCACTGCTTCGCAAAAAAAAAAAAAAAAGTACTCTGCGTTGATACCACTGC2600.11419034652378234No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTGCGAAGCAGTGGTAT2590.11375115288330626No Hit
GCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAAAAGTACTCTG2590.11375115288330626No Hit
ATACCACTGCTTGCAAAAAAAAAAAAAGTACTCTGCGTTGATACCACTGCT2570.11287276560235408No Hit
CCACTGCTTCGCAAAAAAAAAAAAAAAGTACTCTGCGTTGATACCACTGCT2540.11155518468092582No Hit
GTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAGTACTCTGCGTTGA2500.10979841011902149No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2500.10979841011902149No Hit
GCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAGTACTCTGCGTTGAT2500.10979841011902149No Hit
TGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAGTACTCTGCGTTGATA2500.10979841011902149No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTGCGAAGCAGTGGTATC2460.10804163555711713No Hit
TACCACTGCTTCGCAAAAAAAAAAAAAGTACTCTGCGTTGATACCACTGCT2450.10760244191664105No Hit
GATACCACTGCTTCGCAAAAAAAAAAAAAGTACTCTGCGTTGATACCACTG2410.10584566735473672No Hit
CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTGCGAAGCAGTGGTATC2390.10496728007378453No Hit
CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTGCGAAGCAGTG2380.10452808643330845No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTGCGAAGCAGTGG2370.10408889279283236No Hit
AAAAAAAAAGTACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAA2370.10408889279283236No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTGCAAGCAGTGGTATCAACG2360.10364969915235628No Hit
TACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAGTACTCTGCGTTGATACC2310.10145373094997585No Hit
GTTGATACCACTGCTTCGCAAAAAAAAAAAAAAGTACTCTGCGTTGATACC2300.10101453730949976No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2280.10013615002854759No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTTTTC207.021527E-444.9997843
TGCCGTC2450.040.40796345
AATCTCG2550.038.8233436
ATGCCGT2550.038.8233444
TGCAATC2450.038.5712433
CAATCTC2450.038.5712435
GCAATCT2450.038.5712434
GTATGCC2600.038.07673642
ATCTCGT2600.038.07673637
TATGCCG2600.038.07673643
TCTCGTA2600.038.07673638
CGTATGC2600.038.07673641
TCGTATG2600.038.07673640
CGAGACT2600.038.07673623
CTCGTAT2600.038.07673639
TTGCAAT2500.037.79981632
AGACTAC2500.037.79981625
GAGACTA2500.037.79981624
GACTACT2500.037.79981626
GCCCACG2650.037.3583118
Produced by FastQC (version 0.11.2)