FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 36h_13.34100000005c49.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 36h_13.34100000005c49.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences91721
Sequences flagged as poor quality0
Sequence length51
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTC46795.10133993305786TruSeq Adapter, Index 12 (95% over 21bp)
TTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTCT5380.586561419958352TruSeq Adapter, Index 12 (95% over 22bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTGCAAGCAGTGGTATCAACG4060.44264672212470424No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTGCAAGCAGTGGTATCAAC3880.42302199060193413No Hit
GCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAGTACTCTGCGTTGATA3670.40012647049203565No Hit
ATACCACTGCTTGCAAAAAAAAAAAAAAAGTACTCTGCGTTGATACCACTG3620.3946751561801551No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTGCAAGCAGTGGTATCAACGC3450.37614068751976104No Hit
CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTGCAAGCAGTGGTAT3380.3685088474831282No Hit
ATACCACTGCTTGCAAAAAAAAAAAAAAGTACTCTGCGTTGATACCACTGC3290.3586964817217431No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAGTAC3190.34779385309798194No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTGCAAGCAGTGGTATC3120.34016201306134913No Hit
ATACCACTGCTTGCAAAAAAAAAAAAAAAAGTACTCTGCGTTGATACCACT3090.3368912244742207No Hit
GCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAGTACTCTGCGTTGAT3080.3358009616118446No Hit
CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTGCAAGCAGTGGTA3060.33362043588709234No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTGCAAGCAGTGGTATCA3010.3281691215752118No Hit
GATACCACTGCTTGCAAAAAAAAAAAAAAAGTACTCTGCGTTGATACCACT3000.32707885871283565No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAA2970.32380807012570734No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAGTA2780.30309307574056105No Hit
GATACCACTGCTTGCAAAAAAAAAAAAAAAAGTACTCTGCGTTGATACCAC2630.2867391328049193No Hit
GCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAGTACTCTGCGTTGA2610.284558607080167No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTGCAAGCAGTGGTATCAACGC2460.26820466414452526No Hit
GCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAGTACTCTGCGTTGATAC2410.26275334983264464No Hit
CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTGCAAGCAGTGGTATC2370.25839229838314015No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTGCAAGCAGTGGTATCAA2370.25839229838314015No Hit
CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTGCAAGCAGTGGT2240.2442188811722506No Hit
AAAAAAAAGTACTCTGCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAA2110.23004546396136108No Hit
ATACCACTGCTTGCAAAAAAAAAAAAAAAAAGTACTCTGCGTTGATACCAC2050.22350388678710437No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTGCAAGCAGTGGTATCAA2000.21805257247522378No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTGCAAGCAGTGGTAT2000.21805257247522378No Hit
TGATACCACTGCTTGCAAAAAAAAAAAAAAAGTACTCTGCGTTGATACCAC1970.2147817838880954No Hit
AAAGTACTCTGCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAGTACTC1890.20605968098908645No Hit
GCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAGTACTCTGCGTTG1880.20496941812671038No Hit
GCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAGTACTCTGCGTTGATA1880.20496941812671038No Hit
GATACCACTGCTTGCAAAAAAAAAAAAAAGTACTCTGCGTTGATACCACTG1840.20060836667720586No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTGCAAGCAGTGGTATCAACGCA1780.19406678950294914No Hit
ATACCACTGCTTGCAAAAAAAAAAAAAGTACTCTGCGTTGATACCACTGCT1770.19297652664057302No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAGTACT1760.19188626377819692No Hit
AAAGTACTCTGCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAGTACT1710.18643494946631634No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAGT1680.18316416087918796No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTGCAAGCAGTGGTATCAACGCA1640.1788031094296835No Hit
GTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAGTACTCTGCGTTGATAC1600.174442057980179No Hit
TGATACCACTGCTTGCAAAAAAAAAAAAAAAAAGTACTCTGCGTTGATACC1600.174442057980179No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTGCAAGCAGTGGTATCAAC1580.17226153225542679No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTGCGAAGCAGTGGTATCAACG1560.17008100653067457No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTGCGAAGCAGTGGTATCAAC1500.16353942935641783No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTGCAAGCAGTGGTATCAACG1460.15917837790691336No Hit
GTTGATACCACTGCTTGCAAAAAAAAAAAAAAAGTACTCTGCGTTGATACC1450.15808811504453724No Hit
GATACCACTGCTTGCAAAAAAAAAAAAAAAAAGTACTCTGCGTTGATACCA1450.15808811504453724No Hit
AAAAAAAAAGTACTCTGCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAA1440.1569978521821611No Hit
TGATACCACTGCTTGCAAAAAAAAAAAAAAGTACTCTGCGTTGATACCACT1410.15372706359503277No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTGCAAGCAGTGGTATCA1410.15372706359503277No Hit
TGATACCACTGCTTGCAAAAAAAAAAAAAAAAGTACTCTGCGTTGATACCA1380.1504562750079044No Hit
GATACCACTGCTTGCAAAAAAAAAAAAAAAAAAGTACTCTGCGTTGATACC1370.14936601214552828No Hit
GCGTTGATACCACTGCTTGCAAAAAAAAAAAAAGTACTCTGCGTTGATACC1370.14936601214552828No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTGCGAAGCAGTGGTATCA1360.14827574928315215No Hit
TCTTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGT1340.14609522355839993No Hit
GATACCACTGCTTCGCAAAAAAAAAAAAAAGTACTCTGCGTTGATACCACT1320.14391469783364771No Hit
ATACCACTGCTTGCAAAAAAAAAAAAAAAGTACTCTGCGTTGATACCTGTC1300.14173417210889544No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTGCAAGCAGTGGTATCAA1300.14173417210889544No Hit
ACTCTGCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAGTACTCTGCGT1250.13628285779701488No Hit
GATACCACTGCTTGCAAAAAAAAAAAAAGTACTCTGCGTTGATACCACTGC1230.13410233207226263No Hit
ACTCTGCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAGTACTCTGCG1210.13192180634751038No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTGCAAGCAGTGGTATCAAC1210.13192180634751038No Hit
GCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAGTACTCTGCGTTGAT1190.12974128062275814No Hit
CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTGCAAGCAGTGG1190.12974128062275814No Hit
AAAGTACTCTGCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAA1180.12865101776038204No Hit
AAAGTACTCTGCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAGTAC1170.12756075489800592No Hit
GCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAGTACTCTGCGTTGATAC1130.12319970344850144No Hit
GCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAGTACTCTGCGTT1130.12319970344850144No Hit
TTGATACCACTGCTTGCAAAAAAAAAAAAAAAAGTACTCTGCGTTGATACC1130.12319970344850144No Hit
AAAAAAAAAAGTACTCTGCGTTGATACCACTGCTTGCAAAAAAAAAAAAAA1120.1221094405861253No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTGCAAGCAGTGGTATCAACG1120.1221094405861253No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAG1110.1210191777237492No Hit
ATACCACTGCTTGCAAAAAAAAAAAAAAAAGTACTCTGCGTTGATACCTGT1110.1210191777237492No Hit
CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTGCAAGCAGTGGTATCA1090.11883865199899696No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTGCAAGCAGTGGTA1090.11883865199899696No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTGCAAGCAGTGGTATCAAC1070.11665812627424473No Hit
AAAGTACTCTGCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAGTACTCT1050.11447760054949248No Hit
AAAAAAAGTACTCTGCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAA1040.11338733768711637No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAA1030.11229707482474025No Hit
GTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAGTACTCTGCGTTGATA1030.11229707482474025No Hit
GCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAGTACTCTGCGTTGA1030.11229707482474025No Hit
GTTGATACCACTGCTTGCAAAAAAAAAAAAAAGTACTCTGCGTTGATACCA1030.11229707482474025No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1020.11120681196236412No Hit
TTGATACCACTGCTTGCAAAAAAAAAAAAAAAGTACTCTGCGTTGATACCA1020.11120681196236412No Hit
TGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAGTACTCTGCGTTGATAC1020.11120681196236412No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTGCGAAGCAGTGGTATCAACGC1010.11011654909998801No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTGCAAGCAGTGGTATCTGTC990.10793602337523577No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTGCGAAGCAGTGGTATCAACGC980.10684576051285964No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTGCGAAGCAGTGGTATCAA980.10684576051285964No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTGCAAGCAGTGGTATCAACGCAG970.10575549765048353No Hit
ATACCACTGCTTGCAAAAAAAAAAAAAAAAAAGTACTCTGCGTTGATACCA970.10575549765048353No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTGCGAAGCAGTGGTATC970.10575549765048353No Hit
CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT950.10357497192573129No Hit
TGATACCACTGCTTGCAAAAAAAAAAAAAGTACTCTGCGTTGATACCACTG930.10139444620097905No Hit
ACTCTGCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAGTACTCTGCGTT930.10139444620097905No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTGCAAAAAAAAAAAAAGTACTC930.10139444620097905No Hit
GATACCACTGCTTCGCAAAAAAAAAAAAAAAGTACTCTGCGTTGATACCAC930.10139444620097905No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAGTA920.10030418333860293No Hit
AAAAAAAAGTACTCTGCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAG920.10030418333860293No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATACC301.13175025E-437.49999641
TGCCGTC6100.036.88524645
ATGCCGT6350.035.4330744
ACGAGAC6400.035.1562522
ATCTCGT6350.035.0787437
TCTCGTA6350.035.0787438
CGAGACT6300.035.023
AGACTAC6300.035.025
GAGACTA6300.035.024
GACTACT6300.035.026
CTACTTG6250.034.92000228
ACTACTT6250.034.92000227
AGCCCAC6450.034.8837217
GTATGCC6450.034.8837242
GCCCACG6450.034.8837218
CCACGAG6450.034.8837220
TATGCCG6450.034.8837243
GAGCCCA6450.034.8837216
CACGAGA6450.034.8837221
CCCACGA6450.034.8837219
Produced by FastQC (version 0.11.2)