FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 36h_11.34100000005c2f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 36h_11.34100000005c2f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18253
Sequences flagged as poor quality0
Sequence length51
%GC25

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTC224312.28839094943297TruSeq Adapter, Index 12 (95% over 21bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11306.190763162219909No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10155.560729743055936No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5693.117295786993919No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5623.0789459266969814No Hit
GCGTTGATACCACTGCTTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4822.6406618090176956No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4392.4050840957650794No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3051.6709581986522763No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2991.63808688982633No Hit
ATACCACTGCTTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2881.5778228236454281No Hit
CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2851.561387169232455No Hit
GCTTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2561.4025091765737139No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2331.2765024927409192No Hit
TTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTCT2161.183367117734071TruSeq Adapter, Index 12 (95% over 22bp)
GATACCACTGCTTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2131.1669314633210979No Hit
GCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1951.0683175368432587No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1660.9094395441845177No Hit
GCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1550.8491754780036158No Hit
CTGCTTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1510.8272612721196516No Hit
ACCACTGCTTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1400.7669972059387498No Hit
GCGTTGATACCACTGCTTGCGCGAAAAAAAAAAAAAAAAAAAAAAAAAAAA1390.7615186544677587No Hit
CCACTGCTTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1350.7396044485837945No Hit
TGATACCACTGCTTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1330.7286473456418123No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1310.7176902426998302No Hit
ACTCTGCGTTGATACCACTGCTTGCGCAAAAAAAAAAAAAAAAAAAAAAAA1170.6409905221059552No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTGCGCAAAAAAAAAAAAAAAAA1140.624554867692982No Hit
ATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1110.6081192132800087No Hit
AAAGTACTCTGCGTTGATACCACTGCTTGCGCAAAAAAAAAAAAAAAAAAA1080.5916835588670355No Hit
GTTGATACCACTGCTTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA870.4766339779762231No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT850.46567687503424093No Hit
GATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA830.45471977209225883No Hit
TACCACTGCTTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA690.3780200514983838No Hit
ATACCACTGCTTGCGCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA690.3780200514983838No Hit
CTGCTTGCGCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA650.35610584561441955No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAA630.34514874267243745No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT630.34514874267243745No Hit
GCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA510.27940612502054457No Hit
GCTTGCGCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA480.26297047060757134No Hit
TGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA470.25749191913658026No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT470.25749191913658026No Hit
AAAGTACTCTGCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAA460.25201336766558924No Hit
GATACCACTGCTTGCGCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA430.235577713252616No Hit
CCACTGCTTGCGCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA420.23009916178162493No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT410.22462061031063388No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT410.22462061031063388No Hit
ATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTCTTC400.21914205883964277TruSeq Adapter, Index 12 (95% over 24bp)
CCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA400.21914205883964277No Hit
ACTCTGCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAA390.21366350736865175No Hit
GTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA380.20818495589766065No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTGCGCGAAAAAAAAAAAAAAAA380.20818495589766065No Hit
TTGATACCACTGCTTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA360.19722785295567852No Hit
CTGCGTTGATACCACTGCTTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAA360.19722785295567852No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT340.1862707500136964No Hit
ACTCTGCGTTGATACCACTGCTTGCGCGAAAAAAAAAAAAAAAAAAAAAAA330.18079219854270528No Hit
TGCTTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA320.17531364707171423No Hit
TGATACCACTGCTTGCGCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA320.17531364707171423No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT310.16983509560072316No Hit
CTCTGCGTTGATACCACTGCTTGCGCAAAAAAAAAAAAAAAAAAAAAAAAA300.1643565441297321No Hit
CCACTGCTTCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA290.15887799265874103No Hit
CGCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT280.15339944118774998No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT280.15339944118774998No Hit
ACCACTGCTTGCGCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA270.14792088971675887No Hit
TATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTCTT270.14792088971675887RNA PCR Primer, Index 12 (95% over 23bp)
AGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT260.14244233824576782No Hit
TACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA260.14244233824576782No Hit
GTACTCTGCGTTGATACCACTGCTTGCGCAAAAAAAAAAAAAAAAAAAAAA250.13696378677477675No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTC250.13696378677477675No Hit
GTTGATACCACTGCTTGCGCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA250.13696378677477675No Hit
TACCACTGCTTGCGCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA240.13148523530378567No Hit
GCTTCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA230.12600668383279462No Hit
CGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT230.12600668383279462No Hit
TACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA230.12600668383279462No Hit
ACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAAAAAAA210.11504958089081246No Hit
TCTTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGT210.11504958089081246No Hit
AAAGTACTCTGCGTTGATACCACTGCTTGCGCGAAAAAAAAAAAAAAAAAA200.10957102941982139No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA190.10409247794883032No Hit
CTGCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA190.10409247794883032No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC2650.042.4528342
ATCTCGT2650.042.4528337
TGCCGTC2650.042.4528345
TATGCCG2650.042.4528343
TCTCGTA2650.042.4528338
ATGCCGT2650.042.4528344
CGTATGC2650.042.4528341
TCGTATG2650.042.4528340
CTCGTAT2650.042.4528339
AATCTCG2600.042.4038536
TGCAATC2600.042.4038533
CAATCTC2600.042.4038535
GCAATCT2600.042.4038534
AGACTAC2600.042.4038525
TACTTGC2600.042.4038529
CTACTTG2600.042.4038528
ACTACTT2600.042.4038527
GACTACT2600.042.4038526
GCCCACG2700.041.66666818
CCACGAG2700.041.66666820
Produced by FastQC (version 0.11.2)