##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename H23NVBCXX l02n01 36h_11.34100000005c2f.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 18253 Sequences flagged as poor quality 0 Sequence length 51 %GC 25 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.30619624171369 38.0 38.0 38.0 32.0 38.0 2 36.21470443214814 38.0 38.0 38.0 32.0 38.0 3 36.217608064427765 38.0 38.0 38.0 32.0 38.0 4 36.25475264340108 38.0 38.0 38.0 32.0 38.0 5 36.33117843642141 38.0 38.0 38.0 32.0 38.0 6 38.23957705582644 40.0 38.0 40.0 38.0 40.0 7 38.11696707390566 40.0 38.0 40.0 38.0 40.0 8 38.08042513559415 40.0 38.0 40.0 32.0 40.0 9 38.03133731441407 40.0 38.0 40.0 32.0 40.0 10 37.93195639073029 40.0 38.0 40.0 32.0 40.0 11 37.885114775653314 40.0 38.0 40.0 32.0 40.0 12 37.81060647564784 40.0 38.0 40.0 32.0 40.0 13 37.80518270969156 40.0 38.0 40.0 32.0 40.0 14 37.81893387388374 40.0 38.0 40.0 32.0 40.0 15 37.709581986522764 40.0 38.0 40.0 32.0 40.0 16 37.57387826658631 40.0 38.0 40.0 32.0 40.0 17 37.70377472196351 40.0 38.0 40.0 32.0 40.0 18 37.66734235468142 40.0 38.0 40.0 32.0 40.0 19 37.84955897660659 40.0 38.0 40.0 32.0 40.0 20 37.918424368596945 40.0 38.0 40.0 32.0 40.0 21 38.070728099490495 40.0 38.0 40.0 32.0 40.0 22 38.026242261546045 40.0 38.0 40.0 32.0 40.0 23 37.98756368816085 40.0 38.0 40.0 32.0 40.0 24 37.88012929381472 40.0 38.0 40.0 32.0 40.0 25 37.85027118829782 40.0 38.0 40.0 32.0 40.0 26 37.7822275790281 40.0 38.0 40.0 32.0 40.0 27 37.76984605270366 40.0 38.0 40.0 32.0 40.0 28 37.747219635128474 40.0 38.0 40.0 32.0 40.0 29 37.75105462115817 40.0 38.0 40.0 32.0 40.0 30 37.62871856681094 40.0 38.0 40.0 32.0 40.0 31 37.59294362570537 40.0 38.0 40.0 32.0 40.0 32 37.551854489672934 40.0 38.0 40.0 32.0 40.0 33 37.43488741576727 40.0 38.0 40.0 32.0 40.0 34 37.3747877061305 40.0 38.0 40.0 32.0 40.0 35 37.2752972114173 40.0 38.0 40.0 32.0 40.0 36 37.188462170602094 40.0 38.0 40.0 32.0 40.0 37 37.03966471264997 40.0 38.0 40.0 32.0 40.0 38 36.973045526762725 40.0 38.0 40.0 32.0 40.0 39 36.695776036815865 40.0 38.0 40.0 27.0 40.0 40 36.592779269161234 40.0 38.0 40.0 27.0 40.0 41 36.50528680216951 40.0 38.0 40.0 27.0 40.0 42 36.36350189010026 40.0 38.0 40.0 27.0 40.0 43 36.30285432531639 40.0 38.0 40.0 27.0 40.0 44 36.126171040376924 40.0 38.0 40.0 27.0 40.0 45 35.95507587793787 40.0 38.0 40.0 27.0 40.0 46 35.60061359776475 40.0 38.0 40.0 13.0 40.0 47 35.55015613871692 40.0 38.0 40.0 13.0 40.0 48 35.46019832356325 40.0 38.0 40.0 13.0 40.0 49 35.19251629869063 40.0 38.0 40.0 13.0 40.0 50 35.016106941324715 38.0 38.0 40.0 13.0 40.0 51 34.921601928450116 40.0 38.0 40.0 13.0 40.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 2201 1 1.5379431637520682 2201 2 0.9974934134468469 2201 3 0.37940655974513504 2201 4 0.6651439935297248 2201 5 0.9542081595143515 2201 6 0.8225237987526484 2201 7 0.10159252022644694 2201 8 0.7231071440224923 2201 9 0.8787358996665233 2201 10 0.8733625327170529 2201 11 0.7347810556020704 2201 12 1.6033372548261227 2201 13 1.0957525485371846 2201 14 1.265465772170515 2201 15 1.588436099037395 2201 16 1.8592408359758323 2201 17 0.3314354050216721 2201 18 0.704541972481664 2201 19 1.3773465560973577 2201 20 1.6884324931208 2201 21 1.5352427643934448 2201 22 0.9770493812515184 2201 23 1.1243933401578872 2201 24 1.46830605155737 2201 25 0.988417352996855 2201 26 1.3298234973696168 2201 27 1.6809315488116567 2201 28 0.3035789285439705 2201 29 0.24361637613346687 2201 30 0.1188853205215139 2201 31 0.33654639607340187 2201 32 -0.2744016295123046 2201 33 -0.39016135077113034 2201 34 0.5769044079944976 2201 35 0.14884984110813804 2201 36 -0.42149801870085213 2201 37 -0.02046797488036134 2201 38 0.803260346833099 2201 39 -0.08202746545896389 2201 40 -0.13929631499531325 2201 41 0.9119498582811403 2201 42 0.1903811772421804 2201 43 0.68790731018548 2201 44 -1.0911606476326057 2201 45 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2202 16 0.5963008773836975 2202 17 0.32729461827218387 2202 18 -0.06771475629680168 2202 19 0.9653382745238588 2202 20 0.8954718305949214 2202 21 0.25573965880337823 2202 22 0.31245310795959114 2202 23 0.364558971627865 2202 24 0.17016940559463478 2202 25 1.1229929223550386 2202 26 0.1786847810135086 2202 27 0.322753494981427 2202 28 -0.05666951866100334 2202 29 -0.5162179923965553 2202 30 0.027788012032900156 2202 31 -0.415006398957658 2202 32 -0.502144900733839 2202 33 -0.2680081416613973 2202 34 -0.15808524003862345 2202 35 0.23787675622201476 2202 36 0.017425376744284904 2202 37 0.1410227083494462 2202 38 0.4078152122575247 2202 39 1.156067772636277 2202 40 0.02012397485976436 2202 41 -1.2288368912012615 2202 42 -0.8323931498799695 2202 43 -0.2810367891934078 2202 44 0.46577517017277614 2202 45 -1.2808738994576316 2202 46 -0.6386005317118659 2202 47 -1.8529672140670357 2202 48 -0.5800559325450152 2202 49 -1.9461088329689176 2202 50 -3.819291872385584 2202 51 -2.265306460367704 1102 1 -0.1763425505336471 1102 2 -1.8410159033233455 1102 3 0.329717118751347 1102 4 -1.326574432971313 1102 5 -0.21349370383968846 1102 6 -0.1981801349947645 1102 7 0.6378244042844159 1102 8 -1.1505988601183006 1102 9 -0.9514918436046997 1102 10 -0.6442358109682473 1102 11 -0.8532106628244307 1102 12 -0.5208863476583545 1102 13 -0.1920321305518442 1102 14 -0.6517184928398407 1102 15 -0.21487653036219712 1102 16 0.6832573991228301 1102 17 0.9794685313156677 1102 18 0.584459156746675 1102 19 0.008816535393421532 1102 20 -0.4088759954920391 1102 21 -0.3964342542400985 1102 22 -1.6440686311708461 1102 23 0.1036894064104743 1102 24 0.9527781012468068 1102 25 0.9056016180072106 1102 26 0.4395543462308993 1102 27 0.14884045150316894 1102 28 -0.6218869099653475 1102 29 0.3968254858643121 1102 30 -0.18960329231492778 1102 31 -0.3280498772185254 1102 32 -0.28475359638601105 1102 33 0.07981794529512598 1102 34 0.015827803439634636 1102 35 -1.2838623742127737 1102 36 -1.2869224493426685 1102 37 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-0.6333677508506597 1213 30 -0.20651150487531567 1213 31 -1.8618663023393012 1213 32 -1.643449248559925 1213 33 -0.568732779342561 1213 34 -0.8754765443864514 1213 35 -1.4106739684156722 1213 36 -1.2905456377484654 1213 37 -0.2744362288486215 1213 38 -0.6332475896748377 1213 39 -0.03837667180816595 1213 40 -1.0837407594397561 1213 41 -2.3182088718775944 1213 42 -0.5159680291070217 1213 43 -0.2049498326716659 1213 44 0.14693459046263513 1213 45 0.24207296044575344 1213 46 0.7068100963074571 1213 47 -0.47615561986413724 1213 48 -0.7068675267479136 1213 49 -2.1707465141283393 1213 50 -2.2226735148976644 1213 51 -1.3051615328314767 >>END_MODULE >>Per sequence quality scores pass #Quality Count 16 1.0 17 3.0 18 3.0 19 3.0 20 4.0 21 8.0 22 23.0 23 36.0 24 53.0 25 97.0 26 127.0 27 165.0 28 241.0 29 315.0 30 378.0 31 469.0 32 565.0 33 657.0 34 780.0 35 957.0 36 1117.0 37 1410.0 38 2490.0 39 8351.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 36.33075784974519 26.395966902296014 12.576031563373336 24.69724368458546 2 7.795978743220293 27.244836465238592 41.88352599572673 23.075658795814384 3 21.95255574426122 34.83263025256122 34.88193721580014 8.332876787377417 4 8.524626088862105 35.02437955404591 30.866158987563686 25.584835369528296 5 9.37380156686572 32.69051662740372 40.56319509121788 17.372486714512682 6 23.486550156138716 46.37593820193941 11.31320878759656 18.824302854325317 7 10.776310743439435 40.196132142661476 18.320276118994137 30.707280994904945 8 15.931627677642032 37.708869774831534 23.223579685531146 23.13592286199529 9 17.14238755273106 26.965430340218045 22.357968553114556 33.53421355393634 10 11.702185942036927 40.77138004711554 22.308661589875637 25.217772420971897 11 14.863310140798772 28.42272503150167 30.40048211252945 26.31348271517011 12 16.578096751218975 34.443653098120855 26.138169068098392 22.84008108256177 13 19.887141839697584 24.932887744480357 35.966690407056376 19.213280008765683 14 15.942584780584014 27.59546375938202 29.666356215416645 26.79559524461732 15 21.947077192790225 26.28061140634416 26.488796362241825 25.28351503862379 16 20.68814376506684 36.297392066622834 23.044049967126888 19.97041420118343 17 11.702185942036927 45.285706459212186 29.463649810989974 13.548457787760915 18 19.286614432085912 29.976439647142623 38.617062078790205 12.119883841981261 19 12.124034405303238 35.65989152468087 33.39177121569057 18.824302854325317 20 6.261984331342793 30.433353421355392 35.42431381142826 27.880348435873554 21 7.659014956445516 37.17197173067441 34.8490659069742 20.31994740590588 22 3.8569002355777133 48.63857995945872 35.3969210540733 12.107598750890265 23 5.8127431107215255 36.61315948063332 37.4842491645209 20.089848244124255 24 17.498493398345477 40.80972990741248 37.54999178217279 4.141784912069249 25 6.223634471045855 50.950528680216955 36.21322522325097 6.612611625486221 26 17.00635546789393 39.52443567828183 38.24238439623055 5.226824457593689 27 4.50884786062565 51.93666794499534 38.18002520133676 5.374458993042239 28 3.199474059058785 39.60444858379444 38.689530488138935 18.506546869007835 29 4.081520845888347 40.546759436804905 51.449076864077135 3.9226428532296063 30 3.320002191420588 51.85996822440146 39.05659343669534 5.763436147482605 31 5.615515257765847 39.456527694077685 38.41560291458938 16.512354133567083 32 3.4295732208404095 39.64279844409138 51.05462115816578 5.873007176902427 33 5.566208294526928 40.96860790007122 49.70689749630198 3.7582863090998737 34 16.397304552676275 40.990522105955186 36.60220237769134 6.009970963677204 35 3.7199364488029367 42.946364981099 36.48715279680053 16.84654577329754 36 3.435051772311401 55.848353695282974 36.70081630416917 4.015778228236455 37 3.38026625760149 54.90056429080151 37.67599846600559 4.04317098559141 38 3.3473949487755434 41.867090341313755 49.24669917273873 5.538815537171972 39 3.8569002355777133 41.88352599572673 37.01857228948666 17.241001479208897 40 3.8569002355777133 41.675341039829064 48.78650084917548 5.68125787541774 41 4.771818331233222 41.80682627513285 35.60510600997097 17.81624938366296 42 16.7479318468197 41.554812907467266 36.90900126006684 4.788253985646195 43 4.278748698844026 42.710787267846385 48.096203363830604 4.91426066947899 44 4.026735331178436 54.54993699665809 36.2570536350189 5.166274037144579 45 5.182709691557552 41.75204076042294 47.34016326083383 5.725086287185668 46 17.547800361584397 41.14392154714294 34.394346134881935 6.91393195639073 47 4.552676272393579 40.90286528241933 34.70114501725744 19.843313427929655 48 5.966142551909275 40.574152194159865 33.819098230427876 19.640607023502984 49 17.673807045417192 40.69468032652166 35.20517175258861 6.426340875472524 50 4.771818331233222 40.45362406179806 47.59217662849943 7.1823809784692925 51 4.952610529775927 40.25639620884238 35.28187147318249 19.5091217881992 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1189.0 1 655.0 2 121.0 3 244.5 4 368.0 5 341.5 6 315.0 7 309.5 8 304.0 9 305.0 10 306.0 11 678.0 12 1050.0 13 1214.5 14 1379.0 15 1458.0 16 1537.0 17 1442.5 18 1348.0 19 1223.0 20 1098.0 21 1002.5 22 907.0 23 690.5 24 474.0 25 624.0 26 743.0 27 712.0 28 622.5 29 533.0 30 596.0 31 659.0 32 505.5 33 352.0 34 305.5 35 259.0 36 230.5 37 202.0 38 203.5 39 205.0 40 190.0 41 175.0 42 198.0 43 221.0 44 205.0 45 189.0 46 203.0 47 217.0 48 330.5 49 444.0 50 1456.0 51 2468.0 52 1317.0 53 166.0 54 136.5 55 107.0 56 80.0 57 53.0 58 52.0 59 51.0 60 36.0 61 21.0 62 17.5 63 14.0 64 10.5 65 7.0 66 7.5 67 8.0 68 6.0 69 4.0 70 4.5 71 5.0 72 5.5 73 6.0 74 4.0 75 2.0 76 2.0 77 1.0 78 0.0 79 0.0 80 0.0 81 0.5 82 1.0 83 0.5 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02191420588396428 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.00547855147099107 17 0.0 18 0.01095710294198214 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.00547855147099107 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 18253.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 27.557113899085085 #Duplication Level Percentage of deduplicated Percentage of total 1 91.21272365805169 25.135594148907032 2 3.7773359840954273 2.0818495589766064 3 1.1729622266401591 0.9697036103654194 4 0.6759443339960238 0.7450830000547856 5 0.31809145129224653 0.43828411767928555 6 0.25844930417495027 0.4273270147373035 7 0.19880715705765406 0.3834986029693749 8 0.07952286282306163 0.17531364707171423 9 0.13916500994035785 0.34514874267243745 >10 1.4314115308151094 9.225880677148963 >50 0.17892644135188868 3.4788801840793298 >100 0.4572564612326043 26.357311126938036 >500 0.039761431411530816 6.196241713690901 >1k 0.05964214711729623 24.039883854708815 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTC 2243 12.28839094943297 TruSeq Adapter, Index 12 (95% over 21bp) AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1130 6.190763162219909 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1015 5.560729743055936 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 569 3.117295786993919 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 562 3.0789459266969814 No Hit GCGTTGATACCACTGCTTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 482 2.6406618090176956 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 439 2.4050840957650794 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 305 1.6709581986522763 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 299 1.63808688982633 No Hit ATACCACTGCTTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 288 1.5778228236454281 No Hit CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 285 1.561387169232455 No Hit GCTTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 256 1.4025091765737139 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 233 1.2765024927409192 No Hit TTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTCT 216 1.183367117734071 TruSeq Adapter, Index 12 (95% over 22bp) GATACCACTGCTTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 213 1.1669314633210979 No Hit GCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 195 1.0683175368432587 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 166 0.9094395441845177 No Hit GCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 155 0.8491754780036158 No Hit CTGCTTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 151 0.8272612721196516 No Hit ACCACTGCTTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 140 0.7669972059387498 No Hit GCGTTGATACCACTGCTTGCGCGAAAAAAAAAAAAAAAAAAAAAAAAAAAA 139 0.7615186544677587 No Hit CCACTGCTTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 135 0.7396044485837945 No Hit TGATACCACTGCTTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 133 0.7286473456418123 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 131 0.7176902426998302 No Hit ACTCTGCGTTGATACCACTGCTTGCGCAAAAAAAAAAAAAAAAAAAAAAAA 117 0.6409905221059552 No Hit AAAAAGTACTCTGCGTTGATACCACTGCTTGCGCAAAAAAAAAAAAAAAAA 114 0.624554867692982 No Hit ATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 111 0.6081192132800087 No Hit AAAGTACTCTGCGTTGATACCACTGCTTGCGCAAAAAAAAAAAAAAAAAAA 108 0.5916835588670355 No Hit GTTGATACCACTGCTTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 87 0.4766339779762231 No Hit CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 85 0.46567687503424093 No Hit GATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 83 0.45471977209225883 No Hit TACCACTGCTTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 69 0.3780200514983838 No Hit ATACCACTGCTTGCGCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 69 0.3780200514983838 No Hit CTGCTTGCGCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 65 0.35610584561441955 No Hit AAAAAGTACTCTGCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAA 63 0.34514874267243745 No Hit AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 63 0.34514874267243745 No Hit GCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 51 0.27940612502054457 No Hit GCTTGCGCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 48 0.26297047060757134 No Hit TGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 47 0.25749191913658026 No Hit GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT 47 0.25749191913658026 No Hit AAAGTACTCTGCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAA 46 0.25201336766558924 No Hit GATACCACTGCTTGCGCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 43 0.235577713252616 No Hit CCACTGCTTGCGCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 42 0.23009916178162493 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 41 0.22462061031063388 No Hit AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT 41 0.22462061031063388 No Hit ATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTCTTC 40 0.21914205883964277 TruSeq Adapter, Index 12 (95% over 24bp) CCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 40 0.21914205883964277 No Hit ACTCTGCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAA 39 0.21366350736865175 No Hit GTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 38 0.20818495589766065 No Hit AAAAAGTACTCTGCGTTGATACCACTGCTTGCGCGAAAAAAAAAAAAAAAA 38 0.20818495589766065 No Hit TTGATACCACTGCTTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 36 0.19722785295567852 No Hit CTGCGTTGATACCACTGCTTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAA 36 0.19722785295567852 No Hit AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT 34 0.1862707500136964 No Hit ACTCTGCGTTGATACCACTGCTTGCGCGAAAAAAAAAAAAAAAAAAAAAAA 33 0.18079219854270528 No Hit TGCTTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 32 0.17531364707171423 No Hit TGATACCACTGCTTGCGCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 32 0.17531364707171423 No Hit CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 31 0.16983509560072316 No Hit CTCTGCGTTGATACCACTGCTTGCGCAAAAAAAAAAAAAAAAAAAAAAAAA 30 0.1643565441297321 No Hit CCACTGCTTCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 29 0.15887799265874103 No Hit CGCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT 28 0.15339944118774998 No Hit TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 28 0.15339944118774998 No Hit ACCACTGCTTGCGCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 27 0.14792088971675887 No Hit TATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTCTT 27 0.14792088971675887 RNA PCR Primer, Index 12 (95% over 23bp) AGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 26 0.14244233824576782 No Hit TACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 26 0.14244233824576782 No Hit GTACTCTGCGTTGATACCACTGCTTGCGCAAAAAAAAAAAAAAAAAAAAAA 25 0.13696378677477675 No Hit CTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTC 25 0.13696378677477675 No Hit GTTGATACCACTGCTTGCGCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 25 0.13696378677477675 No Hit TACCACTGCTTGCGCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 24 0.13148523530378567 No Hit GCTTCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 23 0.12600668383279462 No Hit CGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 23 0.12600668383279462 No Hit TACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 23 0.12600668383279462 No Hit ACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAAAAAAA 21 0.11504958089081246 No Hit TCTTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGT 21 0.11504958089081246 No Hit AAAGTACTCTGCGTTGATACCACTGCTTGCGCGAAAAAAAAAAAAAAAAAA 20 0.10957102941982139 No Hit CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 19 0.10409247794883032 No Hit CTGCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 19 0.10409247794883032 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0 0.0 0.0 2 0.0 0.0 0.0 3 0.0 0.0 0.0 4 0.0 0.0 0.0 5 0.0 0.0 0.0 6 0.0 0.0 0.0 7 0.0 0.0 0.0 8 0.0 0.0 0.0 9 0.0 0.0 0.0 10 0.0 0.0 0.00547855147099107 11 0.0 0.0 0.01095710294198214 12 0.0 0.0 0.01095710294198214 13 0.0 0.0 0.01095710294198214 14 0.0 0.0 0.01095710294198214 15 0.0 0.0 0.01643565441297321 16 0.0 0.0 0.02191420588396428 17 0.0 0.0 0.02191420588396428 18 0.0 0.0 0.03287130882594642 19 0.0 0.0 0.04382841176792856 20 0.0 0.0 0.04382841176792856 21 0.0 0.0 0.04382841176792856 22 0.0 0.0 0.06026406618090177 23 0.0 0.0 0.08217827206486605 24 0.0 0.0 0.1095710294198214 25 0.0 0.0 0.12052813236180354 26 0.0 0.0 0.19174930148468744 27 0.0 0.0 0.21366350736865172 28 0.0 0.0 0.3396701912014463 29 0.0 0.0 0.48759108091820524 30 0.0 0.0 0.6190763162219909 31 0.0 0.0 0.9203966471264997 32 0.0 0.0 1.1395387059661426 33 0.0 0.0 1.3696378677477674 34 0.0 0.0 1.6873938530652495 35 0.0 0.0 1.9394072207308388 36 0.0 0.0 2.1585492795704817 37 0.0 0.0 2.3612556839971512 38 0.0 0.0 2.5749191913658027 39 0.0 0.0 2.7776255957924723 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATGCC 265 0.0 42.45283 42 ATCTCGT 265 0.0 42.45283 37 TGCCGTC 265 0.0 42.45283 45 TATGCCG 265 0.0 42.45283 43 TCTCGTA 265 0.0 42.45283 38 ATGCCGT 265 0.0 42.45283 44 CGTATGC 265 0.0 42.45283 41 TCGTATG 265 0.0 42.45283 40 CTCGTAT 265 0.0 42.45283 39 AATCTCG 260 0.0 42.40385 36 TGCAATC 260 0.0 42.40385 33 CAATCTC 260 0.0 42.40385 35 GCAATCT 260 0.0 42.40385 34 AGACTAC 260 0.0 42.40385 25 TACTTGC 260 0.0 42.40385 29 CTACTTG 260 0.0 42.40385 28 ACTACTT 260 0.0 42.40385 27 GACTACT 260 0.0 42.40385 26 GCCCACG 270 0.0 41.666668 18 CCACGAG 270 0.0 41.666668 20 >>END_MODULE