##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename H23NVBCXX l02n01 36h_10.34100000005c12.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 48387 Sequences flagged as poor quality 0 Sequence length 51 %GC 25 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.26006985347304 38.0 38.0 38.0 32.0 38.0 2 36.16190299047265 38.0 38.0 38.0 32.0 38.0 3 36.08465083596834 38.0 38.0 38.0 32.0 38.0 4 36.0999441998884 38.0 38.0 38.0 32.0 38.0 5 36.132845599024535 38.0 38.0 38.0 32.0 38.0 6 37.927108520883706 40.0 38.0 40.0 32.0 40.0 7 37.901502469671605 40.0 38.0 40.0 32.0 40.0 8 37.847872362411394 40.0 38.0 40.0 32.0 40.0 9 37.805608944551224 40.0 38.0 40.0 32.0 40.0 10 37.80292227251121 40.0 38.0 40.0 32.0 40.0 11 37.690309380618764 40.0 38.0 40.0 32.0 40.0 12 37.60774588215843 40.0 38.0 40.0 32.0 40.0 13 37.460743588153846 40.0 38.0 40.0 32.0 40.0 14 37.463843594353854 40.0 38.0 40.0 32.0 40.0 15 37.49058631450596 40.0 38.0 40.0 32.0 40.0 16 37.586459172918346 40.0 38.0 40.0 32.0 40.0 17 37.724078781490896 40.0 38.0 40.0 32.0 40.0 18 37.750036166739 40.0 38.0 40.0 32.0 40.0 19 37.75466550933102 40.0 38.0 40.0 32.0 40.0 20 37.71211275755885 40.0 38.0 40.0 32.0 40.0 21 37.69737739475479 40.0 38.0 40.0 32.0 40.0 22 37.63798127596255 40.0 38.0 40.0 32.0 40.0 23 37.596627193254385 40.0 38.0 40.0 32.0 40.0 24 37.51356769380205 40.0 38.0 40.0 32.0 40.0 25 37.40872548411763 40.0 38.0 40.0 32.0 40.0 26 37.19769772872879 40.0 38.0 40.0 32.0 40.0 27 37.02174137681609 40.0 38.0 40.0 32.0 40.0 28 36.93882654431975 40.0 38.0 40.0 32.0 40.0 29 36.861285055903444 40.0 38.0 40.0 32.0 40.0 30 36.75778618223903 40.0 38.0 40.0 32.0 40.0 31 36.58437183541034 40.0 38.0 40.0 27.0 40.0 32 36.458470250273834 40.0 38.0 40.0 27.0 40.0 33 36.32012730692128 40.0 38.0 40.0 27.0 40.0 34 36.1744270155207 40.0 38.0 40.0 27.0 40.0 35 36.00766734866803 40.0 38.0 40.0 27.0 40.0 36 35.78351623369913 40.0 38.0 40.0 27.0 40.0 37 35.63853927707856 38.0 38.0 40.0 27.0 40.0 38 35.53898774464216 38.0 38.0 40.0 27.0 40.0 39 35.270547874429084 38.0 38.0 40.0 13.0 40.0 40 35.16355632711265 38.0 38.0 40.0 13.0 40.0 41 34.96790460247587 38.0 32.0 40.0 13.0 40.0 42 34.845206357079384 38.0 32.0 40.0 13.0 40.0 43 34.738690144046956 38.0 32.0 40.0 13.0 40.0 44 34.57860582387831 38.0 32.0 40.0 13.0 40.0 45 34.4253415173497 38.0 32.0 40.0 13.0 40.0 46 34.242193151052966 38.0 32.0 40.0 13.0 40.0 47 34.196891727116785 38.0 32.0 40.0 13.0 40.0 48 34.088577510488356 38.0 32.0 40.0 13.0 40.0 49 33.975530617727905 38.0 32.0 40.0 13.0 40.0 50 33.90681381362763 38.0 32.0 40.0 13.0 40.0 51 33.90257713848761 38.0 32.0 40.0 13.0 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2201 1 -0.2734309694990884 2201 2 0.12321247905727972 2201 3 0.1353589176632397 2201 4 -0.43464053651951673 2201 5 0.1027063977983147 2201 6 -0.43007702741264353 2201 7 -0.10423070070257978 2201 8 -1.1653035151542426 2201 9 -0.8723555342925948 2201 10 -0.30675200195215524 2201 11 -1.1402981487500625 2201 12 -0.17689631826537067 2201 13 -0.766192775062926 2201 14 -0.571851305861145 2201 15 -1.303026644817436 2201 16 0.0986171973626142 2201 17 -1.2768211887707182 2201 18 -0.5088329249453949 2201 19 -0.24230499832920316 2201 20 -0.20010059787272638 2201 21 -0.10228280387985933 2201 22 0.30144858241528993 2201 23 0.00639652328968765 2201 24 -0.17495748015738855 2201 25 -1.1053574456895632 2201 26 -0.12876974384433737 2201 27 -0.14042538501647073 2201 28 0.2809835140235819 2201 29 0.18110983045711038 2201 30 0.8238618971618976 2201 31 0.17726865785284218 2201 32 -0.08892062019013025 2201 33 0.4060046560942041 2201 34 0.9526120896585084 2201 35 0.37857075510405735 2201 36 1.169344517642422 2201 37 0.30838939695239986 2201 38 0.1475013988022411 2201 39 0.3089574947569602 2201 40 0.47857128933081583 2201 41 0.33429087382001654 2201 42 1.2509476356544056 2201 43 0.20322958123451684 2201 44 -1.1532008485031966 2201 45 -0.7805371669225778 2201 46 0.9227104664144505 2201 47 0.5313294632056937 2201 48 -0.9290154789452529 2201 49 -1.0766528310657222 2201 50 -0.10424834614206446 2201 51 -1.5850838998356878 1101 1 0.66357299875488 1101 2 0.8060001774699757 1101 3 0.17801764782196727 1101 4 1.1154586698296924 1101 5 1.0526071914491055 1101 6 0.8754785281429136 1101 7 0.8357494580275784 1101 8 -0.3800852611859895 1101 9 0.5336464498343858 1101 10 0.9983075218573703 1101 11 0.7411502639483487 1101 12 1.2055441579251038 1101 13 1.0981425423973903 1101 14 0.6315217100118744 1101 15 0.9432531170873233 1101 16 0.883587435457855 1101 17 1.1825042080546808 1101 18 1.2664646941022255 1101 19 1.1542723826231764 1101 20 1.0714767830796532 1101 21 0.4800584659614131 1101 22 0.7067065189232267 1101 23 0.4627457296388968 1101 24 -0.7600269246018314 1101 25 0.6240572368501205 1101 26 0.8685020815524851 1101 27 1.14057659911051 1101 28 1.4940291489442146 1101 29 1.6156336399809206 1101 30 0.8588321352571384 1101 31 0.8030127054718932 1101 32 1.276902792508281 1101 33 0.07961576720531838 1101 34 0.7462628833092992 1101 35 0.5747513106596145 1101 36 0.6375984858963903 1101 37 0.697526301714305 1101 38 0.6182355257863676 1101 39 1.7960606693601378 1101 40 1.795039543299069 1101 41 1.6403424611216053 1101 42 1.591969461051228 1101 43 1.186116485996422 1101 44 0.5266106594333095 1101 45 0.48707196006154874 1101 46 0.9157660219700077 1101 47 -0.10904752092128689 1101 48 -0.3863567487865254 1101 49 -1.8574067993196905 1101 50 -1.382026123919843 1101 51 -0.32169104269283366 2202 1 1.2091087130405924 2202 2 0.7216251774699742 2202 3 1.094089076393395 2202 4 -0.3140056158845965 2202 5 1.053500048591964 2202 6 0.4826213852857677 2202 7 0.8830708865990076 2202 8 0.41723616738543967 2202 9 0.14034287840581783 2202 10 1.0742003790002244 2202 11 0.7724002639483487 2202 12 0.14215130078224547 2202 13 0.4084103995402444 2202 14 1.0868788528690203 2202 15 0.9874495456587553 2202 16 0.893855292600712 2202 17 0.8200042080546837 2202 18 0.8387861226736533 2202 19 1.1957902397660334 2202 20 0.6665660687939408 2202 21 0.03898703738998677 2202 22 0.7665279474946587 2202 23 1.1270314439246079 2202 24 1.4663123611124576 2202 25 1.562896522564408 2202 26 1.4251985101239129 2202 27 0.2722730276819405 2202 28 1.7301898632299313 2202 29 0.7334907828380608 2202 30 0.07624284954285088 2202 31 1.5534591340433224 2202 32 1.5952063639368532 2202 33 1.445687195776749 2202 34 1.3462628833093007 2202 35 1.4341263106596145 2202 36 1.423312771610675 2202 37 1.0845798731428786 2202 38 1.2316283829292232 2202 39 1.7407035265029975 2202 40 1.3753966861562148 2202 41 0.5358781754073192 2202 42 1.0192016039083711 2202 43 1.107991485996422 2202 44 1.7690213737190206 2202 45 1.0464469600615516 2202 46 0.8068374505414369 2202 47 1.4186310505072868 2202 48 1.6154289654991913 2202 49 1.1265217721088803 2202 50 1.3179738760801598 2202 51 1.2863446715928788 1102 1 0.940724638099141 1102 2 0.9363792758306317 1102 3 0.07863240192033061 1102 4 0.1159709649116607 1102 5 -0.4315116610099139 1102 6 -0.5958329472669206 1102 7 -0.142221853447829 1102 8 -0.12445001528435284 1102 9 -0.5931465829524996 1102 10 -0.09441788797869322 1102 11 -0.6128456376909952 1102 12 -1.3072632191240743 1102 13 -0.46512590022555855 1102 14 -0.031388126053698784 1102 15 -0.24603991569956207 1102 16 -0.08951707273886456 1102 17 -0.17507775915843382 1102 18 -0.34880169934039884 1102 19 -0.38594278131125037 1102 20 0.5579009634075192 1102 21 0.15116502333846427 1102 22 0.0904155353166658 1102 23 -0.24627066380372753 1102 24 0.4133849606440734 1102 25 0.31488715488291064 1102 26 0.5654795405688802 1102 27 0.25353766468428063 1102 28 0.47328119812453906 1102 29 0.6627647875219012 1102 30 1.0127768073882848 1102 31 0.6839040989145175 1102 32 1.209023694147625 1102 33 1.1072797016315477 1102 34 1.3036399324896237 1102 35 1.3439623762333852 1102 36 -0.813221186234756 1102 37 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1213 42 -1.702725306390633 1213 43 -0.7175899093524123 1213 44 -0.5638689917294784 1213 45 -1.3163437376128684 1213 46 -1.7426642105881314 1213 47 -1.5807044976654723 1213 48 -1.5825776790190815 1213 49 -2.0797905202499223 1213 50 -2.172723798338449 1213 51 -1.855848019437019 >>END_MODULE >>Per sequence quality scores pass #Quality Count 15 2.0 16 9.0 17 9.0 18 8.0 19 8.0 20 12.0 21 30.0 22 73.0 23 120.0 24 251.0 25 338.0 26 533.0 27 699.0 28 873.0 29 1047.0 30 1283.0 31 1491.0 32 1700.0 33 2184.0 34 2673.0 35 3403.0 36 4550.0 37 4041.0 38 6065.0 39 16985.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.67603770464693 23.982966760377046 15.755746651232016 13.585248883744006 2 10.368487403641474 26.085518837704342 35.984871969743935 27.561121788910242 3 24.506582346498025 31.91766383532767 37.0988075309484 6.476946287225909 4 9.105751544836423 21.85297703928741 37.776675553351104 31.264595862525056 5 10.730154793642921 35.55913778494224 34.30880195093724 19.401905470477608 6 27.740922148510965 44.25362184057701 12.276024552049105 15.729431458862916 7 9.223551780436894 48.500630334594 22.809845619691238 19.465972265277863 8 26.279785892905117 30.444127554921774 22.481244962489924 20.79484158968318 9 14.863496393659453 25.068716804100276 25.81271829210325 34.25506851013702 10 13.123359580052494 38.94434455535578 26.43065286130572 21.501643003286006 11 23.32492869838383 28.793452651593437 22.514777001612035 25.3668416484107 12 19.63957261247856 32.364064728129456 35.29667059334118 12.6996920660508 13 29.11319155971645 22.11544423088846 31.936263872527743 16.835100336867338 14 21.88191043048753 27.098187529708394 35.53020439374212 15.489697646061959 15 18.58557050447434 27.321387976109285 40.0500134333602 14.043028086056172 16 11.054622109244217 41.57728315456631 31.40512947692562 15.962965259263854 17 10.936821873643748 31.66552999772666 43.60675388017442 13.790894248455162 18 10.957715041540942 24.469887984127638 45.6309676352664 18.941429339065017 19 8.7998842664352 37.371608076549485 43.88162109657552 9.946886560439786 20 8.70275073883481 31.791596916527165 46.15909231818463 13.346560026453385 21 6.822080310827289 30.218860437720874 46.13635893938455 16.82270031206729 22 17.10996755326844 26.616653233306465 48.25469650939302 8.018682704032075 23 7.293281253229174 21.716576766486867 48.79203091739517 22.198111062888792 24 10.320953975241284 35.95593857854382 49.490565647797965 4.23254179841693 25 15.617831235662472 28.68952404571476 48.15756298179263 7.53508173683014 26 8.785417570835142 30.313927294521253 53.43170686341373 7.468948271229876 27 18.523570380474094 25.556451112902224 50.28623390580115 5.633744600822535 28 6.681546696426727 25.39731746130159 54.68617603901874 13.234959803252941 29 5.850745034823403 32.62033190733048 55.13257693182053 6.396346126025586 30 7.111708174020874 29.238400330680996 50.191174950914544 13.45871654438359 31 6.481079628825924 24.961249922499846 58.11478289623246 10.442887552441773 32 9.279351892037118 24.066381466096264 56.368446070225474 10.285820571641144 33 6.832413664827329 27.990989315311964 57.74898216463099 7.427614855229711 34 10.914088494843655 28.778390890115112 53.46270692541385 6.8448136896273795 35 7.570215140430281 25.802384938103213 52.49137164940997 14.136028272056544 36 9.463285593237854 28.350590034513402 55.43637753942174 6.7497468328269985 37 7.944282555231777 27.3007212681092 54.20464174261682 10.550354434042202 38 6.636079938826544 28.89825779651559 56.13904561142456 8.326616653233307 39 9.213218426436853 25.70525141050282 53.38417343501354 11.69735672804679 40 6.770413540827082 28.30718994771323 55.26484386302106 9.65755264843863 41 9.655485977638623 25.29605059210118 54.78950957901916 10.258953851241035 42 7.876082418831504 27.470188273709883 54.95897658461983 9.694752722838778 43 5.8714117428234855 31.328662657325314 54.396842127017585 8.403083472833613 44 5.617211234422468 31.208795750924835 51.46010292020584 11.713890094446855 45 6.042945419224171 30.84092834852336 54.06824146981627 9.04788476243619 46 9.531485729638126 29.908859817719634 51.305102610205225 9.254551842437019 47 5.325810651621303 31.77713022092711 50.31930063860127 12.57775848885031 48 5.964411928823857 32.45499824332982 48.3993634653936 13.181226362452724 49 8.039349412032157 33.51726703453407 48.572963812594296 9.87041974083948 50 5.141876950420567 33.951267902535804 50.75950151900304 10.14735362804059 51 5.5056110112220225 34.92053650773968 47.35156136978941 12.22229111124889 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2170.0 1 1234.0 2 298.0 3 543.5 4 789.0 5 868.5 6 948.0 7 1435.5 8 1923.0 9 1706.5 10 1490.0 11 1900.0 12 2310.0 13 2745.0 14 3180.0 15 4202.5 16 5225.0 17 3753.5 18 2282.0 19 2229.5 20 2177.0 21 1930.5 22 1684.0 23 1672.0 24 1660.0 25 1405.0 26 1191.0 27 1232.0 28 2150.0 29 3068.0 30 1978.5 31 889.0 32 713.5 33 538.0 34 926.0 35 1314.0 36 1388.5 37 1463.0 38 1216.5 39 970.0 40 1122.0 41 1274.0 42 1970.5 43 2667.0 44 2347.5 45 2028.0 46 1767.0 47 1506.0 48 1316.0 49 1126.0 50 1654.5 51 2183.0 52 1214.0 53 245.0 54 218.5 55 192.0 56 160.0 57 128.0 58 113.5 59 99.0 60 77.0 61 55.0 62 47.5 63 40.0 64 32.0 65 24.0 66 19.0 67 14.0 68 15.0 69 16.0 70 12.0 71 8.0 72 10.0 73 12.0 74 7.0 75 3.0 76 4.0 77 2.5 78 1.0 79 1.0 80 1.0 81 0.5 82 0.0 83 0.5 84 1.0 85 1.0 86 1.0 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.022733378800090933 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0020666708000082667 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0020666708000082667 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.004133341600016533 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 48387.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 33.54059946021258 #Duplication Level Percentage of deduplicated Percentage of total 1 86.94205395525107 29.16088607961267 2 6.582688410322546 4.415746306840241 3 2.3503948950478426 2.3650096124438424 4 0.9823207430117051 1.3179050633125609 5 0.6197467786558288 1.0393339234826091 6 0.3185106427147945 0.640982273466708 7 0.2389692705623082 0.5610620811060685 8 0.19358236504097098 0.5194294854719893 9 0.09330836625130952 0.28166566848497915 >10 1.226461115063453 8.250400087316894 >50 0.18573840742939485 4.377457009651547 >100 0.17335584693410094 12.60491653158671 >500 0.03714768148587878 7.797604872505453 >1k 0.055721522228818154 26.667601004717717 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2389 4.937276541219749 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2101 4.342075350817368 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1373 2.8375390084113503 No Hit CTTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTC 1334 2.756938847211028 TruSeq Adapter, Index 12 (95% over 21bp) ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1186 2.4510715688098044 No Hit GCTTCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT 1158 2.393204786409573 No Hit GCTTGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT 1142 2.3601380536094405 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1134 2.3436046872093743 No Hit GGTCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1025 2.1183375700084732 No Hit TTTTGGACCTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 699 1.4446028892057785 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 695 1.4363362060057454 No Hit GCGTTGATACCACTGCTTCAAGCAGTGGTATCAACGCAGAGTACTTTTTTT 667 1.378469423605514 No Hit GCGTTGATACCACTGCTTGAAGCAGTGGTATCAACGCAGAGTACTTTTTTT 577 1.19246905160477 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 564 1.1656023312046624 No Hit TTTTTGGACCTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 553 1.1428689524045716 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 476 0.9837353008039349 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 384 0.7936015872031745 No Hit CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 377 0.7791348916031166 No Hit GCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 357 0.7378014756029512 No Hit AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT 328 0.6778680224027115 No Hit GATACCACTGCTTGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTT 276 0.5704011408022817 No Hit CCACTGCTTCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTT 275 0.5683344700022733 No Hit TTTTTGCGCAGGTCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 273 0.5642011284022568 No Hit GCTTAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT 238 0.49186765040196745 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 232 0.47946762560191786 No Hit GATACCACTGCTTCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTT 228 0.4712009424018848 No Hit TGATACCACTGCTTGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTT 217 0.44846756360179385 No Hit CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 206 0.42573418480170294 No Hit CCACTGCTTGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTT 197 0.4071341476016285 No Hit ACTCTGCGTTGATACCACTGCTTGAAGCAGTGGTATCAACGCAGAGTACTT 192 0.39680079360158726 No Hit TTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTCT 186 0.3844007688015376 TruSeq Adapter, Index 12 (95% over 22bp) CTGCTTGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTT 184 0.38026742720152107 No Hit ACTCTGCGTTGATACCACTGCTTCAAGCAGTGGTATCAACGCAGAGTACTT 170 0.35133403600140534 No Hit AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 140 0.2893339120011573 No Hit TGATACCACTGCTTCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTT 135 0.279000558001116 No Hit GCGTTGATACCACTGCTTAAGCAGTGGTATCAACGCAGAGTACTTTTTTTT 135 0.279000558001116 No Hit ATACCACTGCTTGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTT 134 0.27693388720110773 No Hit TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 127 0.26246719160104987 No Hit GTTGATACCACTGCTTGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTT 125 0.2583338500010333 No Hit CTGCTTCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTT 124 0.25626717920102504 No Hit GTTGATACCACTGCTTCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTT 120 0.24800049600099203 No Hit ATACCACTGCTTCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTT 119 0.24593382520098372 No Hit GCGCAGGTCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 115 0.23766714200095068 No Hit GTCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 99 0.20460040920081843 No Hit TTCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT 95 0.19633372600078533 No Hit CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 91 0.18806704280075226 No Hit GCTTGAAGCAGTGGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACA 90 0.186000372000744 No Hit GACCTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 90 0.186000372000744 No Hit AAAAAGTACTCTGCGTTGATACCACTGCTTGAAGCAGTGGTATCAACGCAG 79 0.16326699320065308 No Hit AAAGTACTCTGCGTTGATACCACTGCTTCAAGCAGTGGTATCAACGCAGAG 79 0.16326699320065308 No Hit CTGCTTAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT 79 0.16326699320065308 No Hit AAAAAGTACTCTGCGTTGATACCACTGCTTCAAGCCTGTCTCTTATACACA 76 0.15706698080062828 No Hit GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT 74 0.15293363920061173 No Hit AAAAAGTACTCTGCGTTGATACCACTGCTTCAAGCAGTGGTATCAACGCAG 73 0.15086696840060349 No Hit AAAAAGTACTCTGCGTTGATACCACTGCTTGAAGCCTGTCTCTTATACACA 72 0.1488002976005952 No Hit ATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTCTTC 71 0.14673362680058694 TruSeq Adapter, Index 12 (95% over 24bp) TTTTTTTTTCGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 71 0.14673362680058694 No Hit GCTTCAAGCAGTGGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACA 70 0.14466695600057866 No Hit AAAGTACTCTGCGTTGATACCACTGCTTGAAGCAGTGGTATCAACGCAGAG 70 0.14466695600057866 No Hit AAAAAGTACTCTGCGTTGATACCACTGCTTCAAGCAGTGGTATCAACGCCT 70 0.14466695600057866 No Hit TACCACTGCTTCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTT 67 0.13846694360055387 No Hit GCTTCAAGCAGTGGTATCAACGCAGAGTACTTTCTGTCTCTTATACACATC 67 0.13846694360055387 No Hit CTTCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT 64 0.13226693120052907 No Hit TTTTTTGCGCAGGTCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 63 0.1302002604005208 No Hit GCTTGAAGCAGTGGTATCAACGCAGAGTACTTTCTGTCTCTTATACACATC 62 0.12813358960051252 No Hit CTGCGTTGATACCACTGCTTCAAGCAGTGGTATCAACGCAGAGTACTTTTT 60 0.12400024800049601 No Hit AAAAAGTACTCTGCGTTGATACCACTGCTTGAAGCAGTGGTATCAACGCCT 59 0.12193357720048774 No Hit CGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 54 0.11160022320044641 No Hit ACTCTGCGTTGATACCACTGCTTGAAGCAGTGGTATCAACGCCTGTCTCTT 54 0.11160022320044641 No Hit CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 53 0.10953355240043813 No Hit AGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 53 0.10953355240043813 No Hit CTGCGTTGATACCACTGCTTGAAGCAGTGGTATCAACGCAGAGTACTTTTT 52 0.10746688160042986 No Hit TTGATACCACTGCTTGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTT 51 0.10540021080042161 No Hit CTCTGCGTTGATACCACTGCTTCAAGCAGTGGTATCAACGCAGAGTACTTT 50 0.10333354000041334 No Hit TTTTTTTTTTCGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 49 0.10126686920040506 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0 0.0 0.0 2 0.0 0.0 0.0 3 0.0 0.0 0.0 4 0.0 0.0 0.0 5 0.0 0.0 0.0 6 0.0 0.0 0.0 7 0.0 0.0 0.0 8 0.0 0.0 0.0 9 0.0 0.0 0.0 10 0.0 0.0 0.0248000496000992 11 0.0 0.0 0.045466757600181866 12 0.0 0.0 0.05993345320023973 13 0.0 0.0 0.0744001488002976 14 0.0 0.0 0.12813358960051252 15 0.0 0.0 0.1798003596007192 16 0.0 0.0 0.217000434000868 17 0.0 0.0 0.2542005084010168 18 0.0 0.0 0.3534007068014136 19 0.0 0.0 0.5146010292020584 20 0.0 0.0 0.6406679480025627 21 0.0 0.0 0.7460681588029843 22 0.0 0.0 0.899001798003596 23 0.0 0.0 0.9548019096038192 24 0.0 0.0 1.1180689028044724 25 0.0 0.0 1.1676690020046707 26 0.0 0.0 1.2462024924049848 27 0.0 0.0 1.279269225205117 28 0.0 0.0 1.3268026536053072 29 0.0 0.0 1.5252030504061007 30 0.0 0.0 1.595469857606382 31 0.0 0.0 1.9302705272077212 32 0.0 0.0 2.1824043648087295 33 0.0 0.0 2.5461384256101844 34 0.0 0.0 3.3748734164134997 35 0.0 0.0 3.627007254014508 36 0.0 0.0 4.466075598817865 37 0.0 0.0 4.761609523219047 38 0.0 0.0 5.340277347221361 39 0.0 0.0 5.947878562423791 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGCGCAG 50 1.036824E-9 40.49907 5 TTGCGCA 55 2.6357156E-9 36.817337 4 AATCTCG 165 0.0 34.090126 36 ATCTCGT 165 0.0 34.090126 37 TATGCCG 165 0.0 34.090126 43 CACGAGA 165 0.0 34.090126 21 TCTCGTA 165 0.0 34.090126 38 ACGAGAC 165 0.0 34.090126 22 ATGCCGT 165 0.0 34.090126 44 CGTATGC 165 0.0 34.090126 41 TCGTATG 165 0.0 34.090126 40 CGAGACT 165 0.0 34.090126 23 CTCGTAT 165 0.0 34.090126 39 TTGCAAT 160 0.0 33.749226 32 TGCAATC 160 0.0 33.749226 33 ACTTGCA 160 0.0 33.749226 30 CAATCTC 160 0.0 33.749226 35 GCAATCT 160 0.0 33.749226 34 CGCAGGT 60 6.166374E-9 33.749226 7 AGGTCCA 60 6.166374E-9 33.749226 10 >>END_MODULE