FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 36h_10.34100000005c12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 36h_10.34100000005c12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48387
Sequences flagged as poor quality0
Sequence length51
%GC25

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23894.937276541219749No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA21014.342075350817368No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13732.8375390084113503No Hit
CTTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTC13342.756938847211028TruSeq Adapter, Index 12 (95% over 21bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11862.4510715688098044No Hit
GCTTCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT11582.393204786409573No Hit
GCTTGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT11422.3601380536094405No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11342.3436046872093743No Hit
GGTCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10252.1183375700084732No Hit
TTTTGGACCTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6991.4446028892057785No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6951.4363362060057454No Hit
GCGTTGATACCACTGCTTCAAGCAGTGGTATCAACGCAGAGTACTTTTTTT6671.378469423605514No Hit
GCGTTGATACCACTGCTTGAAGCAGTGGTATCAACGCAGAGTACTTTTTTT5771.19246905160477No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5641.1656023312046624No Hit
TTTTTGGACCTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5531.1428689524045716No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4760.9837353008039349No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3840.7936015872031745No Hit
CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3770.7791348916031166No Hit
GCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3570.7378014756029512No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT3280.6778680224027115No Hit
GATACCACTGCTTGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTT2760.5704011408022817No Hit
CCACTGCTTCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTT2750.5683344700022733No Hit
TTTTTGCGCAGGTCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2730.5642011284022568No Hit
GCTTAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT2380.49186765040196745No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2320.47946762560191786No Hit
GATACCACTGCTTCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTT2280.4712009424018848No Hit
TGATACCACTGCTTGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTT2170.44846756360179385No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2060.42573418480170294No Hit
CCACTGCTTGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTT1970.4071341476016285No Hit
ACTCTGCGTTGATACCACTGCTTGAAGCAGTGGTATCAACGCAGAGTACTT1920.39680079360158726No Hit
TTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTCT1860.3844007688015376TruSeq Adapter, Index 12 (95% over 22bp)
CTGCTTGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTT1840.38026742720152107No Hit
ACTCTGCGTTGATACCACTGCTTCAAGCAGTGGTATCAACGCAGAGTACTT1700.35133403600140534No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1400.2893339120011573No Hit
TGATACCACTGCTTCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTT1350.279000558001116No Hit
GCGTTGATACCACTGCTTAAGCAGTGGTATCAACGCAGAGTACTTTTTTTT1350.279000558001116No Hit
ATACCACTGCTTGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTT1340.27693388720110773No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1270.26246719160104987No Hit
GTTGATACCACTGCTTGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTT1250.2583338500010333No Hit
CTGCTTCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTT1240.25626717920102504No Hit
GTTGATACCACTGCTTCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTT1200.24800049600099203No Hit
ATACCACTGCTTCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTT1190.24593382520098372No Hit
GCGCAGGTCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1150.23766714200095068No Hit
GTCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA990.20460040920081843No Hit
TTCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT950.19633372600078533No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA910.18806704280075226No Hit
GCTTGAAGCAGTGGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACA900.186000372000744No Hit
GACCTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA900.186000372000744No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTGAAGCAGTGGTATCAACGCAG790.16326699320065308No Hit
AAAGTACTCTGCGTTGATACCACTGCTTCAAGCAGTGGTATCAACGCAGAG790.16326699320065308No Hit
CTGCTTAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT790.16326699320065308No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCAAGCCTGTCTCTTATACACA760.15706698080062828No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT740.15293363920061173No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCAAGCAGTGGTATCAACGCAG730.15086696840060349No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTGAAGCCTGTCTCTTATACACA720.1488002976005952No Hit
ATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTCTTC710.14673362680058694TruSeq Adapter, Index 12 (95% over 24bp)
TTTTTTTTTCGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA710.14673362680058694No Hit
GCTTCAAGCAGTGGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACA700.14466695600057866No Hit
AAAGTACTCTGCGTTGATACCACTGCTTGAAGCAGTGGTATCAACGCAGAG700.14466695600057866No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCAAGCAGTGGTATCAACGCCT700.14466695600057866No Hit
TACCACTGCTTCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTT670.13846694360055387No Hit
GCTTCAAGCAGTGGTATCAACGCAGAGTACTTTCTGTCTCTTATACACATC670.13846694360055387No Hit
CTTCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT640.13226693120052907No Hit
TTTTTTGCGCAGGTCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA630.1302002604005208No Hit
GCTTGAAGCAGTGGTATCAACGCAGAGTACTTTCTGTCTCTTATACACATC620.12813358960051252No Hit
CTGCGTTGATACCACTGCTTCAAGCAGTGGTATCAACGCAGAGTACTTTTT600.12400024800049601No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTGAAGCAGTGGTATCAACGCCT590.12193357720048774No Hit
CGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT540.11160022320044641No Hit
ACTCTGCGTTGATACCACTGCTTGAAGCAGTGGTATCAACGCCTGTCTCTT540.11160022320044641No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT530.10953355240043813No Hit
AGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT530.10953355240043813No Hit
CTGCGTTGATACCACTGCTTGAAGCAGTGGTATCAACGCAGAGTACTTTTT520.10746688160042986No Hit
TTGATACCACTGCTTGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTT510.10540021080042161No Hit
CTCTGCGTTGATACCACTGCTTCAAGCAGTGGTATCAACGCAGAGTACTTT500.10333354000041334No Hit
TTTTTTTTTTCGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA490.10126686920040506No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGCAG501.036824E-940.499075
TTGCGCA552.6357156E-936.8173374
AATCTCG1650.034.09012636
ATCTCGT1650.034.09012637
TATGCCG1650.034.09012643
CACGAGA1650.034.09012621
TCTCGTA1650.034.09012638
ACGAGAC1650.034.09012622
ATGCCGT1650.034.09012644
CGTATGC1650.034.09012641
TCGTATG1650.034.09012640
CGAGACT1650.034.09012623
CTCGTAT1650.034.09012639
TTGCAAT1600.033.74922632
TGCAATC1600.033.74922633
ACTTGCA1600.033.74922630
CAATCTC1600.033.74922635
GCAATCT1600.033.74922634
CGCAGGT606.166374E-933.7492267
AGGTCCA606.166374E-933.74922610
Produced by FastQC (version 0.11.2)