##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename H23NVBCXX l02n01 36h_1.34100000005b63.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 21990 Sequences flagged as poor quality 0 Sequence length 51 %GC 21 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.3556161891769 38.0 38.0 38.0 32.0 38.0 2 36.26566621191451 38.0 38.0 38.0 32.0 38.0 3 36.25638926784902 38.0 38.0 38.0 32.0 38.0 4 36.251841746248296 38.0 38.0 38.0 32.0 38.0 5 36.31841746248295 38.0 38.0 38.0 32.0 38.0 6 38.14461118690314 40.0 38.0 40.0 38.0 40.0 7 38.09768076398363 40.0 38.0 40.0 32.0 40.0 8 38.057344247385174 40.0 38.0 40.0 32.0 40.0 9 38.039154160982264 40.0 38.0 40.0 32.0 40.0 10 37.92196452933151 40.0 38.0 40.0 32.0 40.0 11 37.77098681218736 40.0 38.0 40.0 32.0 40.0 12 37.70959527057754 40.0 38.0 40.0 32.0 40.0 13 37.588449295134154 40.0 38.0 40.0 32.0 40.0 14 37.531650750341065 40.0 38.0 40.0 32.0 40.0 15 37.659163256025465 40.0 38.0 40.0 32.0 40.0 16 37.6762619372442 40.0 38.0 40.0 32.0 40.0 17 37.751614370168255 40.0 38.0 40.0 32.0 40.0 18 37.7487494315598 40.0 38.0 40.0 32.0 40.0 19 37.85175079581628 40.0 38.0 40.0 32.0 40.0 20 37.886039108685765 40.0 38.0 40.0 32.0 40.0 21 37.84711232378354 40.0 38.0 40.0 32.0 40.0 22 37.78226466575716 40.0 38.0 40.0 32.0 40.0 23 37.70650295588904 40.0 38.0 40.0 32.0 40.0 24 37.671259663483404 40.0 38.0 40.0 32.0 40.0 25 37.63260572987722 40.0 38.0 40.0 32.0 40.0 26 37.46430195543429 40.0 38.0 40.0 32.0 40.0 27 37.35989085948158 40.0 38.0 40.0 32.0 40.0 28 37.2806275579809 40.0 38.0 40.0 32.0 40.0 29 37.25047748976807 40.0 38.0 40.0 32.0 40.0 30 37.153888130968625 40.0 38.0 40.0 32.0 40.0 31 37.09104138244657 40.0 38.0 40.0 32.0 40.0 32 36.962255570713964 40.0 38.0 40.0 32.0 40.0 33 36.8675306957708 40.0 38.0 40.0 32.0 40.0 34 36.838017280582086 40.0 38.0 40.0 32.0 40.0 35 36.719918144611185 40.0 38.0 40.0 32.0 40.0 36 36.57994542974079 40.0 38.0 40.0 27.0 40.0 37 36.51518872214643 40.0 38.0 40.0 27.0 40.0 38 36.3843565256935 40.0 38.0 40.0 27.0 40.0 39 36.25302410186448 40.0 38.0 40.0 27.0 40.0 40 36.11336971350614 40.0 38.0 40.0 27.0 40.0 41 36.040472942246474 40.0 38.0 40.0 27.0 40.0 42 35.91859936334698 40.0 38.0 40.0 27.0 40.0 43 35.87066848567531 40.0 38.0 40.0 27.0 40.0 44 35.72369258753979 40.0 38.0 40.0 27.0 40.0 45 35.58767621646203 40.0 38.0 40.0 27.0 40.0 46 35.37207821737153 40.0 38.0 40.0 13.0 40.0 47 35.41882673942701 38.0 38.0 40.0 13.0 40.0 48 35.24515688949523 38.0 38.0 40.0 13.0 40.0 49 35.163028649386085 38.0 38.0 40.0 13.0 40.0 50 35.0918599363347 38.0 38.0 40.0 13.0 40.0 51 35.1112778535698 38.0 38.0 40.0 13.0 40.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 2201 1 -0.6401974573708884 2201 2 0.015014448067177 2201 3 1.0497923706152577 2201 4 0.5729733399278274 2201 5 -0.6394884337671058 2201 6 -0.1091417731453106 2201 7 0.6808212748825397 2201 8 0.10132084791983687 2201 9 -0.180258410895064 2201 10 -0.9027483256083855 2201 11 0.04539380922393832 2201 12 -0.6769471127202706 2201 13 -0.9897683888539603 2201 14 0.006718151554544249 2201 15 0.14853818340692015 2201 16 0.32939284890458964 2201 17 -1.066841126791381 2201 18 0.6763559497673342 2201 19 -0.38475393472920416 2201 20 -2.171459218758919 2201 21 -1.1213342181341943 2201 22 -0.8284740909281112 2201 23 -1.6825759185782942 2201 24 -0.12775148717098972 2201 25 0.7714973571175676 2201 26 -1.132154764857674 2201 27 0.4445950153182636 2201 28 -0.3392170008469151 2201 29 0.476424356237942 2201 30 0.1094561060460606 2201 31 0.04402573533941023 2201 32 0.49956667543096245 2201 33 -0.012874908576606003 2201 34 -0.5479419211369674 2201 35 -1.0270258546137967 2201 36 -1.7125329851164537 2201 37 -4.889353214159108 2201 38 -1.7185352433470342 2201 39 -1.3225260537764427 2201 40 -2.979140593602331 2201 41 -2.0971626980240075 2201 42 -2.756917512945712 2201 43 -2.4341269807285 2201 44 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1.8073268256184818 1212 39 1.4679911876028697 1212 40 1.3234456132942256 1212 41 1.6718028192173762 1212 42 0.9827376594680786 1212 43 0.8210454330646044 1212 44 0.9447087660275884 1212 45 0.3027103938912319 1212 46 1.415353923843746 1212 47 1.394377486348148 1212 48 1.1787294031499727 1212 49 0.7114345852632695 1212 50 2.4632090788886174 1212 51 1.7109511371767496 1213 1 -0.8277500225517116 1213 2 -0.7587450136653686 1213 3 -1.3782984620172058 1213 4 -0.41693414535392037 1213 5 -0.29045563309427536 1213 6 -1.4665850027500227 1213 7 0.13162026563106366 1213 8 -0.6118835255032096 1213 9 0.03084335529501203 1213 10 -1.0246995451205834 1213 11 -0.6299636340056693 1213 12 -0.7946931177665277 1213 13 -0.2706767151786025 1213 14 0.17660881597843314 1213 15 -0.4435560133971208 1213 16 -0.2273775463855685 1213 17 -1.7421985700209888 1213 18 -0.19664657336134894 1213 19 -0.46717613826158555 1213 20 -0.11931455938128721 1213 21 -0.6158674393284755 1213 22 0.5415847820744091 1213 23 -0.3999855064672744 1213 24 0.07662193587358956 1213 25 -0.2074765705527426 1213 26 0.13866104674872304 1213 27 -1.362806162141787 1213 28 0.9181421244684742 1213 29 -0.8557875865711395 1213 30 -0.9082057947108808 1213 31 0.21475744265648444 1213 32 -0.030290347277194485 1213 33 0.9862840485302087 1213 34 1.3772052613693404 1213 35 0.08987910753927508 1213 36 0.7239682764478843 1213 37 -0.3536088911986326 1213 38 -0.3938926865766419 1213 39 -1.0643258855678681 1213 40 0.45820171085519945 1213 41 1.5327784289734723 1213 42 0.591274244833933 1213 43 1.0015332379426525 1213 44 -0.4144375754358265 1213 45 0.058807954866843204 1213 46 -0.8200119298147897 1213 47 0.5187677302505875 1213 48 -0.07614864563051782 1213 49 0.612654097458396 1213 50 -0.6209372625747989 1213 51 -0.7012439847744716 >>END_MODULE >>Per sequence quality scores pass #Quality Count 15 2.0 16 2.0 17 6.0 18 3.0 19 6.0 20 8.0 21 16.0 22 32.0 23 51.0 24 82.0 25 141.0 26 236.0 27 240.0 28 333.0 29 355.0 30 477.0 31 578.0 32 663.0 33 788.0 34 1001.0 35 1277.0 36 1624.0 37 1906.0 38 2828.0 39 9335.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.04689987717782 32.07478506118364 12.568803166082882 12.309511895555657 2 7.34912911000955 34.48087680203738 30.115057528764382 28.054936559188686 3 22.132787630741248 41.75534333788086 31.45975443383356 4.6521145975443385 4 9.017735334242838 29.909049567985445 35.10231923601637 25.970895861755345 5 9.936334697589814 40.4456571168713 32.42382901318781 17.194179172351067 6 24.511141427921782 47.5943610732151 12.592087312414735 15.302410186448387 7 6.430195543428831 51.38699408822192 22.219190541155072 19.96361982719418 8 24.89768076398363 34.124602091859934 23.046839472487495 17.93087767166894 9 13.83356070941337 30.89586175534334 25.16143701682583 30.10914051841746 10 13.324238290131879 44.00636653024102 23.70168258299227 18.967712596634833 11 21.61535313111101 34.81286097594251 18.145436354540905 25.426349538405567 12 16.4574806730332 39.445202364711236 33.37880854934061 10.71850841291496 13 27.471577989995456 30.923146884947705 27.698954070031835 13.906321055025012 14 20.58663028649386 34.80673033196908 33.16962255570714 11.437016825829922 15 18.940427467030467 32.364711232378355 38.85402455661664 9.840836743974533 16 9.258753979081401 47.18963165075034 28.708503865393357 14.843110504774899 17 9.176898590268303 36.63028649386085 41.69167803547067 12.501136880400182 18 9.372868252307972 29.319205057073994 43.826458683887395 17.481468006730637 19 7.507958162801273 43.8881309686221 41.41882673942701 7.1850841291496135 20 7.576170986812188 37.9581628012733 43.71987266939518 10.745793542519328 21 4.297407912687586 36.12551159618008 43.73351523419736 15.843565256934971 22 15.025011368804 33.34697589813552 45.67530695770805 5.952705775352433 23 5.1659845384265575 28.335607094133696 45.62528422010004 20.8731241473397 24 7.1168713051387 43.13324238290132 46.780354706684854 2.969531605275125 25 14.824920418371986 33.86994088221919 44.91132332878581 6.39381537062301 26 5.115961800818554 38.05820827648931 50.4274670304684 6.398362892223738 27 17.471577989995453 31.4006366530241 47.27148703956344 3.8562983174170076 28 5.0795816280127335 31.909959072305593 52.39654388358345 10.613915416098227 29 6.03456116416553 39.636198271941794 49.88631195998181 4.442928603910869 30 5.320600272851296 35.4206457480673 47.63074124602092 11.628012733060482 31 4.83856298317417 33.81082310140973 55.320600272851294 6.030013642564802 32 7.644383810823101 31.527967257844473 52.90131878126421 7.926330150068213 33 4.643019554342883 34.738517507958164 54.50204638472032 6.116416552978627 34 7.949067758071851 35.225102319236015 52.064574806730334 4.761255115961801 35 5.375170532060027 32.26011823556162 51.4324693042292 10.932241928149159 36 7.480673033196908 32.487494315598 54.779445202364705 5.252387448840382 37 6.134606639381538 32.50113688040018 53.6243747157799 7.739881764438381 38 5.38881309686221 34.94770350159163 53.465211459754435 6.198271941791724 39 6.894042746703047 31.809913597089583 53.58344702137335 7.712596634834015 40 4.542974079126876 34.26102773988177 52.81036834924966 8.385629831741701 41 6.912232833105958 31.205093224192815 55.25693497044111 6.625738972260119 42 4.506593906321054 32.833105957253295 54.229195088676676 8.431105047748977 43 3.3424283765347886 36.46202819463392 54.04274670304684 6.152796725784448 44 3.0832196452933154 35.1068667576171 52.84220100045475 8.967712596634835 45 3.3469758981355158 37.20782173715325 52.91496134606639 6.530241018644839 46 5.48431105047749 34.59754433833561 52.910413824465664 7.007730786721236 47 2.7785356980445655 37.57162346521146 50.91405184174624 8.735788994997726 48 3.4106412005457027 36.20736698499318 49.57708049113233 10.804911323328787 49 4.547521600727603 38.5993633469759 49.40427467030468 7.448840381991814 50 3.0741246020918602 37.30331969076853 50.80491132332878 8.817644383810823 51 2.8923552685433624 38.81031424803311 48.624312156078034 9.67301832734549 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2743.0 1 1474.0 2 205.0 3 325.5 4 446.0 5 464.0 6 482.0 7 559.5 8 637.0 9 912.0 10 1187.0 11 1304.0 12 1421.0 13 1500.0 14 1579.0 15 1896.5 16 2214.0 17 1708.0 18 1202.0 19 1070.5 20 939.0 21 757.5 22 576.0 23 572.5 24 569.0 25 426.0 26 295.5 27 308.0 28 789.5 29 1271.0 30 759.5 31 248.0 32 171.5 33 95.0 34 219.5 35 344.0 36 436.5 37 529.0 38 470.0 39 411.0 40 562.5 41 714.0 42 949.5 43 1185.0 44 1034.5 45 884.0 46 685.5 47 487.0 48 411.5 49 336.0 50 439.5 51 543.0 52 309.0 53 75.0 54 44.0 55 13.0 56 15.0 57 17.0 58 15.5 59 14.0 60 13.5 61 13.0 62 9.0 63 5.0 64 3.5 65 2.0 66 3.0 67 4.0 68 2.5 69 1.0 70 2.0 71 3.0 72 2.5 73 2.0 74 2.5 75 1.5 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.031832651205093224 2 0.004547521600727603 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.004547521600727603 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.004547521600727603 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.004547521600727603 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 21990.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 25.152341973624377 #Duplication Level Percentage of deduplicated Percentage of total 1 85.57222925329958 21.52341973624375 2 6.129090580365214 3.0832196452933154 3 2.097269933104321 1.5825375170532059 4 1.030555053335744 1.0368349249658937 5 0.7593563550894956 0.9549795361527967 6 0.30735852467908154 0.4638472032742156 7 0.21695895859699874 0.3819918144611187 8 0.48815765684324713 0.9822646657571623 9 0.2350388718134153 0.5320600272851296 >10 2.350388718134153 11.864483856298317 >50 0.4158380039775809 7.662573897226012 >100 0.2711986982462484 16.625738972260116 >500 0.0903995660820828 14.502046384720327 >1k 0.03615982643283312 18.80400181900864 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2679 12.18281036834925 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1456 6.62119145065939 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 797 3.6243747157799002 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 699 3.1787175989085945 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 687 3.1241473396998636 No Hit GCTTCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT 504 2.291950886766712 No Hit GCTTGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT 502 2.282855843565257 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 468 2.1282401091405183 No Hit GCTTGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 373 1.6962255570713962 No Hit CTTATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCGTC 351 1.596180081855389 RNA PCR Primer, Index 27 (95% over 21bp) GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 295 1.3415188722146432 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 283 1.2869486130059118 No Hit GCGTTGATACCACTGCTTCAAGCAGTGGTATCAACGCAGAGTACTTTTTTT 261 1.1869031377899046 No Hit CCACTGCTTGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 247 1.123237835379718 No Hit GCGTTGATACCACTGCTTGAAGCAGTGGTATCAACGCAGAGTACTTTTTTT 228 1.0368349249658937 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 217 0.9868121873578899 No Hit CTGCTTGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 213 0.9686221009549795 No Hit CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 184 0.8367439745338789 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 150 0.6821282401091405 No Hit GCGTTGATACCACTGCTTGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 140 0.6366530241018644 No Hit AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT 132 0.6002728512960437 No Hit CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 114 0.5184174624829468 No Hit GATACCACTGCTTGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTT 98 0.44565711687130516 No Hit AAAAAGTACTCTGCGTTGATACCACTGCTTCAAGCCTGTCTCTTATACACA 94 0.4274670304683947 No Hit CCACTGCTTCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTT 91 0.4138244656662119 No Hit CTGCTTGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTT 90 0.4092769440654843 No Hit GATACCACTGCTTCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTT 90 0.4092769440654843 No Hit GCTTCAAGCAGTGGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACA 87 0.39563437926330147 No Hit TGATACCACTGCTTGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTT 87 0.39563437926330147 No Hit GCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 86 0.3910868576625739 No Hit AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 86 0.3910868576625739 No Hit GCTTGAAGCAGTGGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACA 78 0.35470668485675305 No Hit AAAAAGTACTCTGCGTTGATACCACTGCTTGAAGCCTGTCTCTTATACACA 76 0.3456116416552979 No Hit GATACCACTGCTTGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 75 0.34106412005457026 No Hit CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 74 0.3365165984538427 No Hit GTTGATACCACTGCTTCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTT 64 0.2910413824465666 No Hit TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 61 0.2773988176443838 No Hit TACCACTGCTTGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 59 0.26830377444292863 No Hit GCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 58 0.263756252842201 No Hit ACTCTGCGTTGATACCACTGCTTCAAGCAGTGGTATCAACGCAGAGTACTT 57 0.2592087312414734 No Hit TGATACCACTGCTTCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTT 57 0.2592087312414734 No Hit GCTTGAAGCAGTGGTATCAACGCAGAGTACTTTCTGTCTCTTATACACATC 56 0.2546612096407458 No Hit ACTCTGCGTTGATACCACTGCTTGAAGCAGTGGTATCAACGCAGAGTACTT 56 0.2546612096407458 No Hit AAAGTACTCTGCGTTGATACCACTGCTTGAAGCCTGTCTCTTATACACATC 54 0.24556616643929058 No Hit GCTTCAAGCAGTGGTATCAACGCAGAGTACTTTCTGTCTCTTATACACATC 51 0.2319236016371078 No Hit CCACTGCTTGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTT 49 0.22282855843565258 No Hit AAAGTACTCTGCGTTGATACCACTGCTTCAAGCCTGTCTCTTATACACATC 48 0.21828103683492497 No Hit CTGCTTCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTT 48 0.21828103683492497 No Hit ATACCACTGCTTGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 46 0.20918599363346974 No Hit TTTTTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 46 0.20918599363346974 No Hit ATACCACTGCTTGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTT 45 0.20463847203274216 No Hit TTCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT 42 0.19099590723055934 No Hit TGATACCACTGCTTGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 42 0.19099590723055934 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 41 0.18644838562983176 No Hit GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT 41 0.18644838562983176 No Hit AAAAAGTACTCTGCGTTGATACCACTGCTTGGCAAAAAAAAAAAAAAAAAA 40 0.18190086402910413 No Hit TTTTTTTTTTCGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 40 0.18190086402910413 No Hit AGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 40 0.18190086402910413 No Hit AAAGTACTCTGCGTTGATACCACTGCTTCAAGCAGTGGTATCAACGCCTGT 38 0.17280582082764895 No Hit TTTTTTTTTTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 38 0.17280582082764895 No Hit ACTCTGCGTTGATACCACTGCTTCAAGCAGTGGTATCAACGCCTGTCTCTT 38 0.17280582082764895 No Hit ACTCTGCGTTGATACCACTGCTTGGCAAAAAAAAAAAAAAAAAAAAAAAAA 38 0.17280582082764895 No Hit GCTTAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT 37 0.16825829922692134 No Hit AAAGTACTCTGCGTTGATACCACTGCTTGGCAAAAAAAAAAAAAAAAAAAA 36 0.1637107776261937 No Hit TTATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCGTCT 36 0.1637107776261937 RNA PCR Primer, Index 38 (95% over 21bp) CGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 36 0.1637107776261937 No Hit GTTGATACCACTGCTTGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTT 35 0.1591632560254661 No Hit ATACCACTGCTTCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTT 33 0.15006821282401092 No Hit AAAAAGTACTCTGCGTTGATACCACTGCTTCAAGCAGTGGTATCAACGCCT 33 0.15006821282401092 No Hit TTTTTTTTTTGCGCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 32 0.1455206912232833 No Hit TTTTTTTTTCGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 32 0.1455206912232833 No Hit AAAAAGTACTCTGCGTTGATACCACTGCTTGAAGCAGTGGTATCAACGCCT 32 0.1455206912232833 No Hit ACTCTGCGTTGATACCACTGCTTGAAGCAGTGGTATCAACGCCTGTCTCTT 32 0.1455206912232833 No Hit TTTTTTTTGCGCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 28 0.1273306048203729 No Hit CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 26 0.11823556161891768 No Hit TTGATACCACTGCTTGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTT 26 0.11823556161891768 No Hit AAAAAGTACTCTGCGTTGATACCACTGCTTGAAGCAGTGGTATCAACGCAG 26 0.11823556161891768 No Hit TTTTTTTTTTTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 25 0.11368804001819009 No Hit GCTTGAAGCAGTGGTATCAACGCAGAGTACTTTTTTCTGTCTCTTATACAC 25 0.11368804001819009 No Hit GTTGATACCACTGCTTGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 25 0.11368804001819009 No Hit CCACTGCTTCAAGCAGTGGTATCAACGCAGAGTACTTTTTCTGTCTCTTAT 24 0.10914051841746249 No Hit AAAGTACTCTGCGTTGATACCACTGCTTGAAGCAGTGGTATCAACGCCTGT 23 0.10459299681673487 No Hit TTTTTTTTTGCGCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 23 0.10459299681673487 No Hit CTTCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT 22 0.10004547521600728 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0 0.0 0.0 2 0.0 0.0 0.0 3 0.0 0.0 0.0 4 0.0 0.0 0.0 5 0.0 0.0 0.0 6 0.0 0.0 0.0 7 0.0 0.0 0.0 8 0.0 0.0 0.0 9 0.0 0.0 0.0 10 0.0 0.0 0.009095043201455207 11 0.0 0.0 0.013642564802182811 12 0.0 0.0 0.022737608003638016 13 0.0 0.0 0.031832651205093224 14 0.0 0.0 0.11368804001819009 15 0.0 0.0 0.15006821282401092 16 0.0 0.0 0.16825829922692132 17 0.0 0.0 0.18644838562983174 18 0.0 0.0 0.21373351523419737 19 0.0 0.0 0.2728512960436562 20 0.0 0.0 0.32287403365165984 21 0.0 0.0 0.35470668485675305 22 0.0 0.0 0.4138244656662119 23 0.0 0.0 0.4320145520691223 24 0.0 0.0 0.5411550704865848 25 0.0 0.0 0.5820827648931333 26 0.0 0.0 0.6502955889040473 27 0.0 0.0 0.6957708049113234 28 0.0 0.0 0.768531150522965 29 0.0 0.0 1.0277398817644383 30 0.0 0.0 1.1186903137789905 31 0.0 0.0 1.5461573442473853 32 0.0 0.0 1.8371987266939518 33 0.0 0.0 2.1828103683492497 34 0.0 0.0 3.533424283765348 35 0.0 0.0 3.7744429286039107 36 0.0 0.0 5.5707139608913145 37 0.0 0.0 6.0300136425648025 38 0.0 0.0 6.844020009095043 39 0.0 0.0 7.748976807639837 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGCCGTC 50 2.0008883E-11 45.000004 45 GTATGCC 55 5.4569682E-11 40.909092 42 ATCTCGT 55 5.4569682E-11 40.909092 37 TATGCCG 55 5.4569682E-11 40.909092 43 TCTCGTA 55 5.4569682E-11 40.909092 38 TATCTCG 55 5.4569682E-11 40.909092 36 ATGCCGT 55 5.4569682E-11 40.909092 44 CGTATGC 55 5.4569682E-11 40.909092 41 TCGTATG 55 5.4569682E-11 40.909092 40 CTCGTAT 55 5.4569682E-11 40.909092 39 CTATCTC 55 5.4569682E-11 40.909092 35 GCTATCT 45 1.7846105E-8 40.0 34 ATTGCTA 45 1.7846105E-8 40.0 31 TGCTATC 45 1.7846105E-8 40.0 33 AATTGCT 45 1.7846105E-8 40.0 30 TTGCTAT 45 1.7846105E-8 40.0 32 CGAATTG 40 3.256373E-7 39.375 28 GAATTGC 40 3.256373E-7 39.375 29 GACGAAT 40 3.256373E-7 39.375 26 ACGAATT 40 3.256373E-7 39.375 27 >>END_MODULE