FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 36h_1.34100000005b63.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 36h_1.34100000005b63.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21990
Sequences flagged as poor quality0
Sequence length51
%GC21

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA267912.18281036834925No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14566.62119145065939No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7973.6243747157799002No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6993.1787175989085945No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6873.1241473396998636No Hit
GCTTCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT5042.291950886766712No Hit
GCTTGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT5022.282855843565257No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4682.1282401091405183No Hit
GCTTGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3731.6962255570713962No Hit
CTTATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCGTC3511.596180081855389RNA PCR Primer, Index 27 (95% over 21bp)
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2951.3415188722146432No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2831.2869486130059118No Hit
GCGTTGATACCACTGCTTCAAGCAGTGGTATCAACGCAGAGTACTTTTTTT2611.1869031377899046No Hit
CCACTGCTTGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2471.123237835379718No Hit
GCGTTGATACCACTGCTTGAAGCAGTGGTATCAACGCAGAGTACTTTTTTT2281.0368349249658937No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2170.9868121873578899No Hit
CTGCTTGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2130.9686221009549795No Hit
CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1840.8367439745338789No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1500.6821282401091405No Hit
GCGTTGATACCACTGCTTGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1400.6366530241018644No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT1320.6002728512960437No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1140.5184174624829468No Hit
GATACCACTGCTTGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTT980.44565711687130516No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCAAGCCTGTCTCTTATACACA940.4274670304683947No Hit
CCACTGCTTCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTT910.4138244656662119No Hit
CTGCTTGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTT900.4092769440654843No Hit
GATACCACTGCTTCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTT900.4092769440654843No Hit
GCTTCAAGCAGTGGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACA870.39563437926330147No Hit
TGATACCACTGCTTGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTT870.39563437926330147No Hit
GCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA860.3910868576625739No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT860.3910868576625739No Hit
GCTTGAAGCAGTGGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACA780.35470668485675305No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTGAAGCCTGTCTCTTATACACA760.3456116416552979No Hit
GATACCACTGCTTGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA750.34106412005457026No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA740.3365165984538427No Hit
GTTGATACCACTGCTTCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTT640.2910413824465666No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT610.2773988176443838No Hit
TACCACTGCTTGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA590.26830377444292863No Hit
GCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA580.263756252842201No Hit
ACTCTGCGTTGATACCACTGCTTCAAGCAGTGGTATCAACGCAGAGTACTT570.2592087312414734No Hit
TGATACCACTGCTTCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTT570.2592087312414734No Hit
GCTTGAAGCAGTGGTATCAACGCAGAGTACTTTCTGTCTCTTATACACATC560.2546612096407458No Hit
ACTCTGCGTTGATACCACTGCTTGAAGCAGTGGTATCAACGCAGAGTACTT560.2546612096407458No Hit
AAAGTACTCTGCGTTGATACCACTGCTTGAAGCCTGTCTCTTATACACATC540.24556616643929058No Hit
GCTTCAAGCAGTGGTATCAACGCAGAGTACTTTCTGTCTCTTATACACATC510.2319236016371078No Hit
CCACTGCTTGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTT490.22282855843565258No Hit
AAAGTACTCTGCGTTGATACCACTGCTTCAAGCCTGTCTCTTATACACATC480.21828103683492497No Hit
CTGCTTCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTT480.21828103683492497No Hit
ATACCACTGCTTGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA460.20918599363346974No Hit
TTTTTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA460.20918599363346974No Hit
ATACCACTGCTTGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTT450.20463847203274216No Hit
TTCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT420.19099590723055934No Hit
TGATACCACTGCTTGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA420.19099590723055934No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT410.18644838562983176No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT410.18644838562983176No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTGGCAAAAAAAAAAAAAAAAAA400.18190086402910413No Hit
TTTTTTTTTTCGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA400.18190086402910413No Hit
AGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT400.18190086402910413No Hit
AAAGTACTCTGCGTTGATACCACTGCTTCAAGCAGTGGTATCAACGCCTGT380.17280582082764895No Hit
TTTTTTTTTTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA380.17280582082764895No Hit
ACTCTGCGTTGATACCACTGCTTCAAGCAGTGGTATCAACGCCTGTCTCTT380.17280582082764895No Hit
ACTCTGCGTTGATACCACTGCTTGGCAAAAAAAAAAAAAAAAAAAAAAAAA380.17280582082764895No Hit
GCTTAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT370.16825829922692134No Hit
AAAGTACTCTGCGTTGATACCACTGCTTGGCAAAAAAAAAAAAAAAAAAAA360.1637107776261937No Hit
TTATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCGTCT360.1637107776261937RNA PCR Primer, Index 38 (95% over 21bp)
CGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT360.1637107776261937No Hit
GTTGATACCACTGCTTGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTT350.1591632560254661No Hit
ATACCACTGCTTCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTT330.15006821282401092No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCAAGCAGTGGTATCAACGCCT330.15006821282401092No Hit
TTTTTTTTTTGCGCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA320.1455206912232833No Hit
TTTTTTTTTCGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA320.1455206912232833No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTGAAGCAGTGGTATCAACGCCT320.1455206912232833No Hit
ACTCTGCGTTGATACCACTGCTTGAAGCAGTGGTATCAACGCCTGTCTCTT320.1455206912232833No Hit
TTTTTTTTGCGCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA280.1273306048203729No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT260.11823556161891768No Hit
TTGATACCACTGCTTGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTT260.11823556161891768No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTGAAGCAGTGGTATCAACGCAG260.11823556161891768No Hit
TTTTTTTTTTTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA250.11368804001819009No Hit
GCTTGAAGCAGTGGTATCAACGCAGAGTACTTTTTTCTGTCTCTTATACAC250.11368804001819009No Hit
GTTGATACCACTGCTTGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA250.11368804001819009No Hit
CCACTGCTTCAAGCAGTGGTATCAACGCAGAGTACTTTTTCTGTCTCTTAT240.10914051841746249No Hit
AAAGTACTCTGCGTTGATACCACTGCTTGAAGCAGTGGTATCAACGCCTGT230.10459299681673487No Hit
TTTTTTTTTGCGCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA230.10459299681673487No Hit
CTTCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT220.10004547521600728No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC502.0008883E-1145.00000445
GTATGCC555.4569682E-1140.90909242
ATCTCGT555.4569682E-1140.90909237
TATGCCG555.4569682E-1140.90909243
TCTCGTA555.4569682E-1140.90909238
TATCTCG555.4569682E-1140.90909236
ATGCCGT555.4569682E-1140.90909244
CGTATGC555.4569682E-1140.90909241
TCGTATG555.4569682E-1140.90909240
CTCGTAT555.4569682E-1140.90909239
CTATCTC555.4569682E-1140.90909235
GCTATCT451.7846105E-840.034
ATTGCTA451.7846105E-840.031
TGCTATC451.7846105E-840.033
AATTGCT451.7846105E-840.030
TTGCTAT451.7846105E-840.032
CGAATTG403.256373E-739.37528
GAATTGC403.256373E-739.37529
GACGAAT403.256373E-739.37526
ACGAATT403.256373E-739.37527
Produced by FastQC (version 0.11.2)