FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 30h_96.34100000005b57.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 30h_96.34100000005b57.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences326
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGATATAAATCTCGTATGCCGTC11234.355828220858896No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTC144.294478527607362No Hit
CTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTC123.6809815950920246No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGTC103.067484662576687No Hit
CTTATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCGTC92.7607361963190185RNA PCR Primer, Index 27 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGTC61.8404907975460123No Hit
CTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTC61.8404907975460123No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTC51.5337423312883436No Hit
CTTATACACATCTCCGAGCCACGAGACAGATATAAATCTCGTATGCCGTCT20.6134969325153374No Hit
CTTATACACATCTCCGAGCCCACGAGACCAAGGCGAATCTCGTATGCCGTC20.6134969325153374No Hit
TTATACACATCTCCGAGCCCACGAGACAGATATAAATCTCGTATGCCGTCT20.6134969325153374No Hit
TCCCAGACCTATGTAAAGTTCCTTCGAAGAGTCGAGTTGTTTGGGAATGCA20.6134969325153374No Hit
TTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTCT20.6134969325153374Illumina PCR Primer Index 11 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACTGTGACCTATCTCGTATGCCGTC10.3067484662576687TruSeq Adapter, Index 4 (95% over 21bp)
CAATCAAAGCAAATGAGATTAAGAGCCCTGATCAAAAGAAATACGGACAAC10.3067484662576687No Hit
CAGGAAACCTTAGTCTTTCGGCGGATAGGATTCTCACCTATCTTTCGCTAC10.3067484662576687No Hit
CTTATACACATCTCCGCGCCCACGAGACAGATATAAATCTCGTATGCCGTC10.3067484662576687No Hit
CTCTTAGTATACACCTTTAATCCCAAACAATGAAGGTAAAGTTAGTTTGTA10.3067484662576687No Hit
CCTTTTTGCCACTTGGGAGTTGGGCGCACACAGACTGGGTTCCCTCCTGCA10.3067484662576687No Hit
CATTCCACCGCTACACCAGGAATTCCACTTACCTCTCCTTAACTCCTGTCT10.3067484662576687No Hit
GCGTTGATACCACTGCTTTGCAAAAAAAAAAAAAAAAAAAAAAGACCCCTG10.3067484662576687No Hit
GTTCGTCCTCCGCCTCCGCTTCTCGCCGGGGGCTGGCCGCTGTCCGGTCTC10.3067484662576687No Hit
GCCCAGATCACCAGTTAAGGTCCCCAAATCTATGCTAAGTGGAAAAGGATG10.3067484662576687No Hit
CTCTTTGAATGATGGCTGCTTCTAAGCCAACGTCCTAGTTGTCTACGCAAC10.3067484662576687No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTATTAGAATCA10.3067484662576687No Hit
GTAGAAAATGCAACAAAATATGGGCTTCTCAATGTTCCCTGGAGTGTGAGA10.3067484662576687No Hit
ATCCAGCAATATTTACCATTGGCAAGCCTATTCTAGGAATTTGCTATGGCA10.3067484662576687No Hit
GATCTGGTTGGAACAGGATAGTAATGACCATCTAATGAAACCTAAATGCAC10.3067484662576687No Hit
GGGTGAGAATCCCGTGCGATGAGATGTCCCAGACCTATGTAAAGTTCCTTC10.3067484662576687No Hit
CATGAGTGGTGAGGAGGGCATGTTAGTTAGCAAGTACATAACATTTTCATC10.3067484662576687No Hit
CCCTAAATGGAAAATGCTCTTAAGAAAGGAGAATAAGGGGCTGTCTCTTAT10.3067484662576687No Hit
ACGTCACAGAGGGTGAGAATCCCGTGCGATGAGATGTCCCAGACCTATGTA10.3067484662576687No Hit
TGCCAAGGCTGTGGGCAAGGTCATCCCAGAGCTGAACGGGAAGCTCACTGG10.3067484662576687No Hit
GAGGCGAAGAAGGACGTACAAGCAACGAAAACCTCGAATAGGAGCAAATAT10.3067484662576687No Hit
TTTGTTACAGAACCCATCTAAAGGACTATCTTTTCTCCACTTAATTACCTT10.3067484662576687No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTATTTTTTTCCTTGTCTCATA10.3067484662576687No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA10.3067484662576687No Hit
CATGGAAGTGAGTGGAAGTAAAAAGAAAAAACCCTCTGTGGGAAGAGATCC10.3067484662576687No Hit
CTTATACACATCACCGAGCCCACGAGACGAATTGCTATCTCGTATGCCGTC10.3067484662576687RNA PCR Primer, Index 27 (95% over 21bp)
CATGTGTAAGCGTCAAGGCTTGAAGTGAGTCAAATGCCTGTCTCTTATACA10.3067484662576687No Hit
TGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACTGTCTCTTATACA10.3067484662576687No Hit
CTCCACAACTGCATTCACTATCACTTCTTGAGATGGGACAGATATCCGTGC10.3067484662576687No Hit
TTTCGGTACTGCTTCTGCCTTCATACAATGCTCCTCTACCACTTGTTATTA10.3067484662576687No Hit
CCTAAAAATCTTCTCACTATTTTTGCCACATAGACGAGTTGATTCATAAAA10.3067484662576687No Hit
CTTATACACATCTCAGACCCACGAGACGACGCTATATCTCGTATGCCGTCT10.3067484662576687TruSeq Adapter, Index 11 (95% over 21bp)
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTCGAAGAAGTGGTTT10.3067484662576687No Hit
ATACACATCTCCGAGCCCACGAGACAGATATAAATCTCGTATGCCGTCTTC10.3067484662576687RNA PCR Primer, Index 39 (95% over 23bp)
TTAGTCTACATATTGAGGACTTGCCTAAAAAACAACTCAGTTAATTGATCA10.3067484662576687No Hit
GCCTTTTAGAAGCAAGCATTTGACTCACTTCAAGCCTTGACGCTTACACAT10.3067484662576687No Hit
AAGTGGATGTTTCTCATTTTCCATGATTTTCAGTTTTTTTGCCATATTCCA10.3067484662576687No Hit
CTTATACACATCTCCGAGCCTACGAGACTGCGACCTATCTCGTATGCCGTC10.3067484662576687No Hit
GTGTTAGCTCTGTGAATATGGAACTCACCAGGGAAGTCCATGGGCTGTCTC10.3067484662576687No Hit
TCACAGAAGAGCAAGAGGTGACACACAGTTTACTTCTACAGGCTCAAGTAA10.3067484662576687No Hit
CTTATACACATCTCCGAGCCCACGAGACCAGCATCAATCTCGTATGCCGTC10.3067484662576687No Hit
CATCAAATCCAACAGTGACAGAAGATGGTAAGAGGATACCACCAAGTAGCT10.3067484662576687No Hit
CTTATACACATCTCCGAGCCCACGAAACTGCCACCTATCTCGTATGCCGTC10.3067484662576687No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTGCGAAGAAGGGGG10.3067484662576687No Hit
GGGTGAGAATCCCGTGCGATGAGATGTCCCAGACCTATGTAACTGTCTCTT10.3067484662576687No Hit
GTTAAGGTCCCCAAATCTATGCTAAGTGGAAAAGGATGTGGAGTTGCGTAG10.3067484662576687No Hit
CGATGATAGGGTTGCTGCTGTTCGGGGAGAAGCGGACACAAGACACCCATT10.3067484662576687No Hit
TAACGTAACTCGCCGGTTCATTCTACAAAAGGCACGCCATTACACTTTAAT10.3067484662576687No Hit
TTACATAGGTCTGGGACATCTCATCGCACGGGATTCTCACTGTCTCTTATA10.3067484662576687No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGATCATATCTCGTATGCCGTC10.3067484662576687TruSeq Adapter, Index 9 (95% over 21bp)
CTTATACACATCTACGAGCCCACGAGACTAGATCCTATCTCATATGCCGTC10.3067484662576687No Hit
TTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGTCT10.3067484662576687Illumina Paired End PCR Primer 2 (95% over 21bp)
CCTTAACTGGTGATCTGGGCTGTTTCCCTTTCGACTACGGATCTTATCCTG10.3067484662576687No Hit
TTTTTGCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTT10.3067484662576687No Hit
GGTGGAAACTATAGTCATGTATTAAGATTGGGGTTTGCAATCGTATAGAAT10.3067484662576687No Hit
TTACGGAGGTAGAGCACTGAATTTCTAAGGGGGCGTCAAAGCTTACCAAGG10.3067484662576687No Hit
TTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGTCT10.3067484662576687No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTACCAC10.3067484662576687No Hit
CTCCTATGGGATGAACAAATGTTTATCAGGAGGGGAAGTTGATTCATCGTG10.3067484662576687No Hit
TAACTGAACATCTCACGACACGAGCTGACGACAACCATGCACCACCTGTAT10.3067484662576687No Hit
CATTCGCTACTCATGCCTACATTCTCACTCGCATACCCTCCACCACTAGAT10.3067484662576687No Hit
CTTATACACATCTCCGAGCCAACGAGACTAGATCCTATCTCGTATGCCGTC10.3067484662576687No Hit
CTTATACACATCTCCGAACCCACGAGACTAGATCCTATCTCGTATGCCGTC10.3067484662576687No Hit
CTCTCCTACCACGTGCTTTCGCACATCCACAGTTTCGGTACTATGCTTAGC10.3067484662576687No Hit
GTACTTATCAGCTCAGTTTCCTCATAGTCTTTGTTCACACCCTGTCTCTTA10.3067484662576687No Hit
CCCCAAATCTATGCTAAGTGGAAAAGGATGTGGAGTTGCGTAGACAACTAG10.3067484662576687No Hit
CTTTACATAGGTCTGGGACATCTCATCGCACGGGATTCTCACCCTCTGTGA10.3067484662576687No Hit
CATTCAAAGAGTGCGTAATAGCTCACTAGTCGAGTGGCGCTGCGCCTGTCT10.3067484662576687No Hit
GGGCTAAGCATAGTACCGAAACTGTGGATGTGCGAAAGCACGTGGTAGGAG10.3067484662576687No Hit
GATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAATCCTTCGTTCTGGA10.3067484662576687No Hit
TGCTAAACATACGCCAAATGTTGGTGAAAGGTATCCTGTAGTTACTTTACC10.3067484662576687No Hit
CTTATACAATCTCCGAGCCCACGAGACCAAGGCGAATCTCGTATGCCGTCT10.3067484662576687No Hit
TTATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCGTCT10.3067484662576687RNA PCR Primer, Index 38 (95% over 21bp)
ATTTGTGACATGAATTTGGTGGTAACTTCTTGGTCTCATACTTCTGTCTCT10.3067484662576687No Hit
CTACTCAAGATACTGAACATGACATGGTAATTTCCACCAGCCCCAGTGGCT10.3067484662576687No Hit
CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTCCAA10.3067484662576687No Hit
ATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAACTGTCC10.3067484662576687No Hit
GAATTAATGCTCACCAATTCAGGTGTAATGAGAGAAGGATCCATAACTGTC10.3067484662576687No Hit
GATGTGTATCTGTCTCTATACACATCTCCGAGCCCACGAGACTAGATCCTA10.3067484662576687No Hit
GTACGGGAAGCATGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA10.3067484662576687No Hit
TTTTTGATGCGTACTGGACCCAGCCGAGCCTTTCCTTCTGGCTAGCCTTTT10.3067484662576687No Hit
GTACTATGGTACACAAGAAGCTAGAGCAGTTATGCTACTGTCTCTTATACA10.3067484662576687No Hit
TCACAAGACTATGTATTCAACTTGTGATGACTGTGTCTTCCACAGCCGGGT10.3067484662576687No Hit
GAAGTAGTATTCCTGCAATCCCGTGGAAATGAGGTTTGCCCTGTCTCTTAT10.3067484662576687No Hit
GGTGAGAATCCTATCCGCCGAAAGACTAAGGTTTCCTGCTGTCTCTTATAC10.3067484662576687No Hit
TCTGTTGGGTGTCCAGCAACTTCTGGTCCTGTTACCTGTCTCTTATCACAT10.3067484662576687No Hit
CTGTGGATGTGCGAAAGCACGTGGTAGGAGAGCGTTCTAAGTGCTGTATCT10.3067484662576687No Hit
GTTTCATGTAGTGTATCATCTCGCATTAACCCCAGTTTATTGAATCCTGCA10.3067484662576687No Hit
GTCCTAAAGTGTGTATTTCTCATTTTTCGTGATTTTAAGTTTTCTTGCCAT10.3067484662576687No Hit
GACTGCGAGTGATAAGATCCGTAGTCGAAAGGGAAACAGCCCAGATCACCA10.3067484662576687No Hit
GCTTCTTCTCTACTTGTTTTTCGATCCCCATCATAACTCACTTTAGCAAGT10.3067484662576687No Hit
CCATCAGTTCAAGCAGGTGCTGAAGAAGCAGCTCAAACAGGTATCTTAGGC10.3067484662576687No Hit
GAGTCAGACGGCGAGTGATAAGATCCGTCGTCGAAAGGGAAACAGCCCAGC10.3067484662576687No Hit
GTAGGATGGTGAGTGGCACTGGCTCCTCTTTGATCCCAGGCCCCTGTCTCT10.3067484662576687No Hit
CTTATACACATCTCCGCGCCCACGAGACTGCGACCTATCTCGTATGCCGTC10.3067484662576687No Hit
GCTTCGCAAAAAAAAAAAAAAAAAAAAAAAATACCTTCGTTGGTTACCCCC10.3067484662576687No Hit
GTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAT10.3067484662576687No Hit
TCCTAGAGCAAGAGGTCCCATTGGGTGGTTTGCACCAAGTTGCATTCCTTT10.3067484662576687No Hit
TTCCCAAACAACTCGACTCTTCGAAGGAACTTTACATAGGTCTGGGACCTG10.3067484662576687No Hit
GGGTGAGAATCCCGTGCGATGAGATGTCCCAGACCTATGTAAAGTTCCTGT10.3067484662576687No Hit
CTTATACACATCTCCGAGCCCACGAGACAGATATAAATTCGTATGCCGTCT10.3067484662576687No Hit
GTACTCAAGACTGTACCACCAGACTCCATAAATGACTTGTTCATGGCACGT10.3067484662576687No Hit
TCCTACAGTGGACATTTCTAAATGTCCACTCTAGGACCTGTCTCTTATACA10.3067484662576687No Hit
ATGTAAAGTTCCTTCGAAGAGTCGAGTTGTTTGGGAATGCAGCTCTAAGTG10.3067484662576687No Hit
AAATGGAGACGTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATC10.3067484662576687No Hit
GAATGGAATGGAATGGAACGGAACGGAATGGAATCGAATGGAATGGAATCT10.3067484662576687No Hit
CTGTTTGGACTAGGGGCCCGTCAGGGGTTACTGAATCCAGATAAACTCCGA10.3067484662576687No Hit
CTTATACACATCTCCGAGCCCAAGACCAAGGCGAATCTCGTATGCCGTCTT10.3067484662576687No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCTG10.3067484662576687No Hit
ACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGCAGGCGCGGGTAAC10.3067484662576687No Hit
TCTCTAGCCTTTACAACACCCATCAGGACTTTAGGCACTCCAACACCTGTC10.3067484662576687No Hit
CGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTTTTTTATAC10.3067484662576687No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTCTGAAGACTGTGTCT10.3067484662576687No Hit
CTTACACACATCTCCGAGCCCACGAGACAGATATAAATCTCGTATGCCGTC10.3067484662576687No Hit
TTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGCC10.3067484662576687No Hit
GAGTGATAAGATCCGTAGTCGAAAGGGAAACAGCCCAGATCACCAGTTAAG10.3067484662576687No Hit
GTTCAGTTTGGTTCCCTCATCGGGTAATAATAATAAGTGTTGGGATTAAGG10.3067484662576687No Hit
TTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTCT10.3067484662576687Illumina PCR Primer Index 8 (95% over 21bp)
CCCCTTAACGGGCTCTGACTACTTGTAAGCACAAGGTTTCAGGTCCCGTAC10.3067484662576687No Hit
CATCTACTCCGCCCTCATCCTGCACGACGACGAGGTGACGGTCACGGAGGA10.3067484662576687No Hit
CGTCAGATGTGTATAAGAGACAGCTTATACACATCTCCGAGCCCACGAGAC10.3067484662576687No Hit
CACCAGAAGAGGGCATCAGATCTCATTACGGGTGGTTGTGAGCCACCATGT10.3067484662576687No Hit
AAGGAGGTGGACGAACAGATGCTTAATGTCCAAAACAAGAACAGCAGCTAC10.3067484662576687No Hit
TTCCATCACTGTACTTGTTCGCTATCGGTCTCTCGCCAATATCTGTCTCTT10.3067484662576687No Hit
ACTCATACCGGCATTCTCACTTCTAAGCGCTCCATCTATCCTCTCGATTAG10.3067484662576687No Hit
CCTCAAATCTGTTAACAATAGAAGGCTTAAACAAGAATGGATGCTGCTGGA10.3067484662576687No Hit
TCCTTGCTCCAGGCAAGATGATCCTCAACACTGTCTATTGAAAGAGCTGTC10.3067484662576687No Hit
CTTATACACATCTCCGAGCCCACGAGACAGATATAAATCTCGTATGCCGCC10.3067484662576687No Hit
CTTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTC10.3067484662576687TruSeq Adapter, Index 12 (95% over 21bp)
GCTAAATGCTATCTGTTCAGAACCTCCTTCACTTTTTTTCCTGTCTCTTAT10.3067484662576687No Hit
GTGTAAGCGTCAAGGCTTGAAGTGAGTCAAATGCTTGCTTCTAAAACTGTC10.3067484662576687No Hit
CGGAGATGTGTATAAGAGACAGGGACATGGGTAGGTCACTTGGTTTCGGGT10.3067484662576687No Hit
CTTATACACATCTCCGAGCCCACGAGACGACGCTAGATCTCGTATGCCGTC10.3067484662576687No Hit
CCTCCAGACTTCTACAACCAGCAAGGTCAGGATTGGGGTCAGCCTCCATTT10.3067484662576687No Hit
CTATCAAGACCAAAGCGCCCGTACTCTGCGTTGATACCCTGTCTCTTAACA10.3067484662576687No Hit
AAAATGTACTCTGCGTTGATACCACTGCTTGCGCAAAAAAAAAAAAAAAAA10.3067484662576687No Hit
ATCTATACGCGGGAGTCAGACTGCGAGTGATAAGATCCGTAGTCGAAAGGG10.3067484662576687No Hit
GTACGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA10.3067484662576687No Hit
CAGTAGGCTAATGGCGCATGTAACTGGAATCATGTGTAAGCGTCAAGGCTT10.3067484662576687No Hit
CTTATACACACTCCGAGCCCACGAGACAGATATAAATCTCGTATGCCGTCT10.3067484662576687No Hit
CCTCTATTTCCAAGGAATGTTTCTGTCCGTATGTGAAGCTGTCTCTTATAC10.3067484662576687No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTCGAAGGAGTGG10.3067484662576687No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCCCAC150.003679487644.99999617
GTATGCC150.003679487644.99999642
ATCTCGT150.003679487644.99999637
ATCTCCG150.003679487644.99999610
CACATCT150.003679487644.9999967
GCCCACG150.003679487644.99999618
TATACAC150.003679487644.9999963
TGCCGTC150.003679487644.99999645
CCACGAG150.003679487644.99999620
TATGCCG150.003679487644.99999643
CATCTCC150.003679487644.9999969
GAGCCCA150.003679487644.99999616
CACGAGA150.003679487644.99999621
CCCACGA150.003679487644.99999619
ACATCTC150.003679487644.9999968
TTATACA150.003679487644.9999962
TACACAT150.003679487644.9999965
ACACATC150.003679487644.9999966
TCTCGTA150.003679487644.99999638
ACGAGAC150.003679487644.99999622