##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename H23NVBCXX l02n01 30h_95.34100000005b4a.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 109544 Sequences flagged as poor quality 0 Sequence length 51 %GC 31 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.331894033447746 38.0 38.0 38.0 32.0 38.0 2 36.266066603373986 38.0 38.0 38.0 32.0 38.0 3 36.171985686117 38.0 38.0 38.0 32.0 38.0 4 36.22282370554298 38.0 38.0 38.0 32.0 38.0 5 36.23667202220113 38.0 38.0 38.0 32.0 38.0 6 38.179151756371866 40.0 38.0 40.0 38.0 40.0 7 38.02472066019134 40.0 38.0 40.0 32.0 40.0 8 37.956428467099975 40.0 38.0 40.0 32.0 40.0 9 37.94972796319287 40.0 38.0 40.0 32.0 40.0 10 37.826024245965094 40.0 38.0 40.0 32.0 40.0 11 37.85720806251369 40.0 38.0 40.0 32.0 40.0 12 37.8012214270065 40.0 38.0 40.0 32.0 40.0 13 37.8028280873439 40.0 38.0 40.0 32.0 40.0 14 37.826471554809025 40.0 38.0 40.0 32.0 40.0 15 37.88348061053093 40.0 38.0 40.0 32.0 40.0 16 37.83169320090557 40.0 38.0 40.0 32.0 40.0 17 37.83922442123713 40.0 38.0 40.0 32.0 40.0 18 37.87499087124808 40.0 38.0 40.0 32.0 40.0 19 37.91629847367268 40.0 38.0 40.0 32.0 40.0 20 37.88719601256116 40.0 38.0 40.0 32.0 40.0 21 37.9577247498722 40.0 38.0 40.0 32.0 40.0 22 37.94403162199664 40.0 38.0 40.0 32.0 40.0 23 37.95594464324837 40.0 38.0 40.0 32.0 40.0 24 37.96945519608559 40.0 38.0 40.0 32.0 40.0 25 37.97889432556781 40.0 38.0 40.0 32.0 40.0 26 37.92358321770248 40.0 38.0 40.0 32.0 40.0 27 37.873475498429855 40.0 38.0 40.0 32.0 40.0 28 37.800664573139564 40.0 38.0 40.0 32.0 40.0 29 37.669794785656904 40.0 38.0 40.0 32.0 40.0 30 37.54745125246476 40.0 38.0 40.0 32.0 40.0 31 37.33531731541664 40.0 38.0 40.0 32.0 40.0 32 37.09978638720514 40.0 38.0 40.0 32.0 40.0 33 36.77109654568027 40.0 38.0 40.0 32.0 40.0 34 36.47973417074417 40.0 38.0 40.0 27.0 40.0 35 36.20353465274228 40.0 38.0 40.0 27.0 40.0 36 35.86411852771489 40.0 38.0 40.0 27.0 40.0 37 35.62552033885927 40.0 38.0 40.0 13.0 40.0 38 35.38365405681735 40.0 38.0 40.0 13.0 40.0 39 35.031165559044766 40.0 38.0 40.0 13.0 40.0 40 34.68101402176295 40.0 38.0 40.0 13.0 40.0 41 34.33048820565252 38.0 32.0 40.0 13.0 40.0 42 33.88838275031038 38.0 32.0 40.0 13.0 40.0 43 33.484271160446944 38.0 32.0 40.0 13.0 40.0 44 32.9154494997444 38.0 27.0 40.0 13.0 40.0 45 32.28856897684948 38.0 27.0 40.0 13.0 40.0 46 31.520594464324837 38.0 27.0 40.0 13.0 40.0 47 30.71625100416271 38.0 13.0 40.0 13.0 40.0 48 29.95275870882933 38.0 13.0 40.0 13.0 40.0 49 29.239565836558825 38.0 13.0 40.0 13.0 40.0 50 28.61282224494267 32.0 13.0 40.0 13.0 40.0 51 27.817936171766597 32.0 13.0 40.0 13.0 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2201 1 -0.16754096333273338 2201 2 -0.34477398149322624 2201 3 -0.4321994679009933 2201 4 0.2043509423110592 2201 5 -0.11766203529778352 2201 6 -0.01724018688299367 2201 7 -0.3133777637168862 2201 8 -0.0024130955159265 2201 9 -0.17809001476351227 2201 10 -0.022935496601725447 2201 11 0.23627619335182715 2201 12 -0.16426209921499435 2201 13 -0.20716690102830881 2201 14 0.6625829253410416 2201 15 0.11658576229392281 2201 16 -0.5234954749208782 2201 17 -0.17273424894744238 2201 18 0.4317248522939252 2201 19 0.1197191345498183 2201 20 -0.42729093151143616 2201 21 0.6624084956239855 2201 22 -0.11386241838091138 2201 23 -0.10104259003118443 2201 24 -0.4849924806259125 2201 25 0.09249982590019812 2201 26 -0.543184460477022 2201 27 -0.5250318384629864 2201 28 -0.8942045405060384 2201 29 -0.29838498913114364 2201 30 -0.7492074502746888 2201 31 0.03772484269613585 2201 32 -0.32588175333241765 2201 33 -0.21557972963107375 2201 34 0.22757921320595642 2201 35 0.5888127472225904 2201 36 0.44244733117015755 2201 37 0.1893878074837474 2201 38 -0.5682067767956198 2201 39 0.526678939763535 2201 40 0.5862528758679133 2201 41 1.3216965427598026 2201 42 0.7467612158599479 2201 43 0.7693637933564759 2201 44 0.8986005149182787 2201 45 0.8959036877318454 2201 46 0.364213479428102 2201 47 0.12405837022404853 2201 48 0.05565800492950146 2201 49 -0.18707092466126696 2201 50 0.6792328259330951 2201 51 1.1789022447899384 1101 1 0.7223371355578294 1101 2 0.32627564641163076 1101 3 -0.019881520587837542 1101 4 0.0906678851955931 1101 5 0.37651684430775134 1101 6 0.5970135683103592 1101 7 0.01141792637123018 1101 8 0.19230908865975493 1101 9 0.24646369933957857 1101 10 0.4476849663485538 1101 11 0.5837171831705703 1101 12 0.44387377258338745 1101 13 0.42845379020120333 1101 14 1.1117451405627037 1101 15 0.41033547694487993 1101 16 0.55174535966799 1101 17 0.6092590852990085 1101 18 0.19143813357959516 1101 19 0.4337857007736261 1101 20 0.43880047149271917 1101 21 0.847725575476062 1101 22 0.4380254508607493 1101 23 0.2895888303221881 1101 24 0.09095601957953647 1101 25 0.17773929081367612 1101 26 0.8393202192472202 1101 27 0.44236988732799887 1101 28 0.24563931649984738 1101 29 0.051019202075544 1101 30 0.3657996263814738 1101 31 0.809308644001888 1101 32 0.7494960487951445 1101 33 1.171536190562506 1101 34 1.0092986123750194 1101 35 0.9389930878152057 1101 36 0.9462139384842274 1101 37 1.0881687963893825 1101 38 1.2504938578206435 1101 39 1.2948842084481882 1101 40 1.150422167745738 1101 41 1.443780275581787 1101 42 1.2515779076373263 1101 43 1.6151383447877876 1101 44 1.3952448102202908 1101 45 1.8788284012414067 1101 46 2.3628438040411694 1101 47 1.82204494740526 1101 48 1.4549640360667873 1101 49 1.3597948014949672 1101 50 1.405389060238896 1101 51 2.4663971085072376 2202 1 0.32068509632189546 2202 2 0.48175663075167563 2202 3 0.44574401718534773 2202 4 0.28624523849106964 2202 5 0.3204593671093292 2202 6 0.5267158570730501 2202 7 0.8005678145144017 2202 8 0.5749024460853249 2202 9 0.49603348078855447 2202 10 0.2241864290916311 2202 11 0.6719119861304748 2202 12 0.6739116318158835 2202 13 0.44144639044212397 2202 14 0.7341423183290061 2202 15 0.5650420678930814 2202 16 0.9088697788729476 2202 17 0.5088460754900197 2202 18 0.280364308076237 2202 19 0.3381911387361072 2202 20 0.37856987434937395 2202 21 0.1416078676804986 2202 22 0.1812710196096745 2202 23 -0.1143863890892689 2202 24 0.429581041090465 2202 25 0.4236091927238519 2202 26 0.5816193071838924 2202 27 0.9181916047604517 2202 28 0.5049320372019679 2202 29 0.09761187115142889 2202 30 0.47266591445581696 2202 31 0.14251291176991288 2202 32 0.12950809491457704 2202 33 -0.24723854700417291 2202 34 -0.5343255486988028 2202 35 0.24776094188506903 2202 36 0.21023906324760588 2202 37 -0.2937362113545561 2202 38 -0.45321462608918495 2202 39 0.32848427728315244 2202 40 0.5014805053812594 2202 41 -0.37793715687389806 2202 42 0.14406629173645058 2202 43 0.3500806955019442 2202 44 -0.36421999790223225 2202 45 0.13661535701493932 2202 46 0.7429418938708068 2202 47 0.5407250368907164 2202 48 0.8103675810676485 2202 49 0.8364769560294683 2202 50 -0.25417900033587415 2202 51 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445.0 25 716.0 26 1054.0 27 1521.0 28 2016.0 29 2757.0 30 3801.0 31 4640.0 32 5588.0 33 6229.0 34 7402.0 35 8107.0 36 9614.0 37 11552.0 38 13269.0 39 30269.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 57.264832385144324 12.869496927137078 13.681317176070937 16.184353511647657 2 11.101880591564726 25.705678291035237 50.13237173635202 13.06006938104802 3 22.401044329219307 38.733294383991826 28.83407580515592 10.031585481632952 4 9.49664061929453 20.43562404148105 35.04527860950851 35.02245672971591 5 16.142372014898125 36.982399766303956 28.70353465274228 18.171693566055648 6 24.315343606222157 41.14145913970642 12.017089023588696 22.526108230482727 7 25.67735339224421 32.363251296282776 21.482691886365295 20.47670342510772 8 29.80902650989557 26.131965237712702 18.116008179361717 25.943000073030014 9 29.198313006645733 15.651245161761484 24.162893449207623 30.98754838238516 10 23.630687212444315 31.505148616081208 22.62287300080333 22.241291170671147 11 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24 18.498502884685607 30.345797122617395 43.808880449864894 7.346819542832105 25 9.481121741035565 37.94913459431826 44.91984955816841 7.649894106477763 26 13.766283559880597 33.7328720228586 44.340578585578264 8.160265831682535 27 6.706894033447747 39.16508434966771 44.242496165924194 9.885525450960344 28 6.258672314321186 34.0319871467173 41.977652815307096 17.73168772365442 29 7.0528737311034835 36.085043452859125 40.728839553056304 16.133243262981082 30 8.878867273440996 40.9545284414055 39.910170621046 10.256433664107503 31 15.413897611918498 32.877200029212005 39.67446870663843 12.034433652231066 32 10.142956255020813 33.73530270941357 38.92773679982473 17.19400423574089 33 9.338713211129775 40.629336157160594 38.493208208573726 11.53874242313591 34 14.529321551157526 37.28547432994961 38.59453735485284 9.590666764040021 35 8.305338494121084 39.69729058643102 37.14215292485211 14.85521799459578 36 8.2852552399036 46.44526400350544 37.09650916526692 8.172971591324034 37 7.679106112612284 47.2549842985467 37.60132914627912 7.464580442561893 38 7.895457533046082 40.60468852698459 42.69973709194479 8.800116848024537 39 7.662674359161616 40.80825969473453 36.85642298984883 14.672642956255022 40 7.498356824654934 40.98992185788359 41.807858029650184 9.70386328781129 41 8.424012269042576 40.83838457606076 34.11688453954576 16.62071861535091 42 14.226246987511868 40.6795442927043 34.21091068429124 10.883298035492588 43 7.858029650186227 41.135981888556195 38.474037829547946 12.531950631709632 44 8.031475936609947 45.97513327977799 32.55586796173227 13.437522821879794 45 8.70700357847075 38.767983641276565 37.50547725115022 15.019535529102463 46 15.089826918863652 37.83319944497188 29.331592784634488 17.74538085152998 47 8.877041473055751 37.13038953451219 28.605727535808512 25.38684145662355 48 10.22146352150734 36.31691375155189 27.25662747389177 26.204995253049002 49 16.256481413861096 35.384868180822316 28.07091214489155 20.287738260425034 50 9.854487694442415 34.593405389615135 34.037464397867524 21.51464251807493 51 9.898847888404024 34.15344446676039 27.729189870182037 28.218517774653545 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 8.0 1 12.0 2 16.0 3 31.0 4 46.0 5 69.0 6 92.0 7 108.5 8 125.0 9 203.5 10 282.0 11 638.0 12 994.0 13 2315.0 14 3636.0 15 5302.5 16 6969.0 17 7693.0 18 8417.0 19 8100.5 20 7784.0 21 7498.5 22 7213.0 23 7940.5 24 8668.0 25 8828.5 26 6881.5 27 4774.0 28 3643.0 29 2512.0 30 2365.5 31 2219.0 32 3590.5 33 4962.0 34 5328.5 35 5695.0 36 5454.5 37 5214.0 38 4240.5 39 3267.0 40 2705.0 41 2143.0 42 1982.0 43 1821.0 44 1759.0 45 1697.0 46 1644.5 47 1592.0 48 1647.5 49 1703.0 50 1677.0 51 1651.0 52 2175.5 53 2700.0 54 5535.5 55 8371.0 56 4816.5 57 1262.0 58 1176.5 59 1091.0 60 1021.5 61 952.0 62 860.0 63 768.0 64 663.5 65 559.0 66 480.0 67 401.0 68 342.5 69 284.0 70 247.0 71 210.0 72 182.0 73 154.0 74 144.5 75 97.5 76 60.0 77 54.0 78 48.0 79 39.5 80 31.0 81 22.5 82 14.0 83 12.0 84 10.0 85 6.5 86 3.0 87 2.0 88 1.0 89 1.0 90 1.0 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03377638209304024 2 0.0036515007668151606 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0018257503834075803 12 0.0 13 0.0 14 0.0 15 0.0 16 9.128751917037902E-4 17 0.0 18 0.002738625575111371 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 9.128751917037902E-4 27 0.0 28 0.0 29 0.0 30 0.002738625575111371 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.002738625575111371 48 0.0 49 0.0 50 0.0 51 0.005477251150222742 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 109544.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.5756878494532 #Duplication Level Percentage of deduplicated Percentage of total 1 87.5029619643697 40.7551064235506 2 6.88770471291696 6.415991694160561 3 2.053222534017997 2.868907554896566 4 0.9513105392611289 1.7723177089808535 5 0.5940592488394326 1.3834359069013031 6 0.28929378292028 0.8084434158049464 7 0.2532858997696887 0.8257875503038639 8 0.193046103703622 0.7193004053322456 9 0.1590687145186307 0.6667861314630176 >10 0.8786450232655664 7.930163298788756 >50 0.12068976102607429 3.985400513085012 >100 0.08110475318489686 7.341267324847791 >500 0.019781645669995046 6.494552121059072 >1k 0.01384715196899653 11.769245111988734 >5k 0.0019781645669995043 6.2632948388366545 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACCAGCAGCAATCTCGTATGCCGTC 6798 6.205725553202366 No Hit GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2388 2.179945957788651 No Hit GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA 2361 2.1552983276126487 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1995 1.8211860074490616 No Hit GTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1901 1.7353757394289053 No Hit GTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1501 1.3702256627473892 No Hit TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA 1483 1.3537939092967208 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1145 1.0452420945008398 No Hit TTATACACATCTCCGAGCCCACGAGACCAGCAGCAATCTCGTATGCCGTCT 902 0.8234134229168188 RNA PCR Primer, Index 36 (95% over 21bp) GAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 803 0.7330387789381436 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 803 0.7330387789381436 No Hit GGTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA 786 0.7175199006791791 No Hit ATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAA 714 0.6517928868765063 No Hit GAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA 698 0.6371868838092456 No Hit GTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA 657 0.5997590009493902 No Hit GTATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAA 603 0.5504637405973855 No Hit GTACGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA 564 0.5148616081209377 No Hit GTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 519 0.4737822244942671 No Hit TATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAA 411 0.3751917037902578 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 403 0.3678887022566275 No Hit TATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAA 386 0.35236982399766303 No Hit GAGTACGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA 346 0.3158548163295114 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTT 344 0.3140290659461038 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTTT 295 0.26929818155261814 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 295 0.26929818155261814 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGT 288 0.2629080552106916 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTTT 230 0.20996129409187178 No Hit GTTCTATAGCTCCTAGATGTACGAATTTCTTTCTCCAATACCTGTCTCTTA 218 0.19900679179142627 No Hit GGTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA 204 0.18622653910757322 No Hit ATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA 196 0.1789235375739429 No Hit GGTATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAA 196 0.1789235375739429 No Hit GTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG 192 0.17527203680712775 No Hit GCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA 189 0.17253341123201635 No Hit GTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT 185 0.16888191046520118 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 185 0.16888191046520118 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG 184 0.1679690352734974 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTTTT 183 0.1670561600817936 No Hit GTACGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 179 0.16340465931497847 No Hit ATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAA 177 0.16157890893157087 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTT 177 0.16157890893157087 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 173 0.1579274081647557 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTT 165 0.1506244066311254 No Hit TCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA 165 0.1506244066311254 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTTTT 162 0.14788578105601402 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGT 146 0.13327977798875337 No Hit GTATCAACGCAGAGTACGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAA 140 0.12780252683853063 No Hit GTATCAACGCAGAGTACGGGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA 140 0.12780252683853063 No Hit GTATCAACGCAGAGTACGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAA 139 0.12688965164682683 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGT 136 0.12415102607171548 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTT 131 0.11958665011319652 No Hit GTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC 121 0.11045789819615862 No Hit GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAG 120 0.10954502300445483 No Hit GTATTGGAGAAAGAAATTCGTACATCTAGGAGCTATAGAACCTGTCTCTTA 117 0.10680639742934345 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGT 115 0.10498064704593589 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTT 112 0.1022420214708245 No Hit GTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGT 111 0.10132914627912072 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0 0.0 0.0 2 0.0 0.0 0.0 3 0.0 0.0 0.0 4 0.0 0.0 0.0018257503834075805 5 0.0 0.0 0.0045643759585189516 6 0.0 0.0 0.0045643759585189516 7 0.0 0.0 0.005477251150222741 8 0.0 0.0 0.006390126341926532 9 0.0 0.0 0.009128751917037903 10 0.0 0.0 0.010041627108741694 11 0.0 0.0 0.010041627108741694 12 0.0 0.0 0.010954502300445482 13 0.0 0.0 0.012780252683853063 14 0.0 0.0 0.012780252683853063 15 0.0 0.0 0.013693127875556854 16 0.0 0.0 0.015518878258964435 17 0.0 0.0 0.017344628642372014 18 0.0 0.0 0.020996129409187176 19 0.0 0.0 0.02647338055940992 20 0.0 0.0 0.03195063170963266 21 0.0 0.0 0.04473088439348572 22 0.0 0.0 0.06116263784415395 23 0.0 0.0 0.12688965164682683 24 0.0 0.0 0.17618491199883152 25 0.0 0.0 0.21178704447527935 26 0.0 0.0 0.2921200613452129 27 0.0 0.0 0.356934199956182 28 0.0 0.0 0.45643759585189514 29 0.0 0.0 0.6216680055502811 30 0.0 0.0 0.7695537866062951 31 0.0 0.0 0.9247425691959396 32 0.0 0.0 1.104578981961586 33 0.0 0.0 1.3474037829547945 34 0.0 0.0 1.636785218724896 35 0.0 0.0 1.8695683926093625 36 0.0 0.0 2.112393193602571 37 0.0 0.0 2.477543270284087 38 0.0 0.0 2.8399547213904914 39 0.0 0.0 3.412327466588768 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGCCGTC 785 0.0 39.839954 45 GCCGTCT 85 0.0 39.70508 45 ATGCCGT 805 0.0 39.129642 44 GTATGCC 815 0.0 38.92559 42 CTCGTAT 815 0.0 38.92559 39 TATGCCG 810 0.0 38.8881 43 TCTCGTA 810 0.0 38.8881 38 GCAGCAA 810 0.0 38.8881 31 CGTATGC 810 0.0 38.8881 41 TCGTATG 810 0.0 38.8881 40 AGCAATC 800 0.0 38.81171 33 AATCTCG 795 0.0 38.772797 36 CACGAGA 825 0.0 38.726486 21 ACGAGAC 825 0.0 38.726486 22 CAGCAGC 805 0.0 38.57065 29 CAGCAAT 805 0.0 38.57065 32 ATCTCGT 820 0.0 38.413857 37 CGAGACC 815 0.0 38.37345 23 GACCAGC 810 0.0 38.332554 26 GAGACCA 810 0.0 38.332554 24 >>END_MODULE