FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 30h_94.34100000005b3d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 30h_94.34100000005b3d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences151194
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCAGCAGCAATCTCGTATGCCGTC2200.14550841964628225No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC250.00210133636.00000445
GCCCACG300.005133290330.00000218
ATGCCGT300.005133290330.00000244
GGTTATA300.005133290330.0000021
CTAGAAC450.001221631425.03
TTTGTCC500.002255590422.50000234
GCCATCC500.002255590422.5000023
TCTCCAC500.002255590422.5000023
GTGTTGG754.568763E-521.01
GGTCAAG550.003920983520.4545461
TTACACT550.003920983520.4545464
GTATCAA1552.3646862E-1120.322581
TCTCGTA600.006484973218.75000238
ACACATC3550.018.38028145
GGTATCA850.0025513715.8823531
AACGCAG2051.2823875E-915.3658546
ATCTCGT900.0037338715.037
TATCAAC2152.5120244E-914.6511632
CAACGCA2203.4742698E-914.3181825
ACTGTGT950.00534749314.2105268
Produced by FastQC (version 0.11.2)