FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 30h_93.34100000005b20.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 30h_93.34100000005b20.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences455242
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG800.039.3747143
ACGAGAC950.035.52605422
CCACGAG950.035.52605420
GCCCACG1200.029.9997818
CCCACGA1200.029.9997819
GCGCTTA300.00514421529.9997813
TGCCGTC1050.029.99977945
CACGAGA1150.029.34761221
CGAGCCC1150.029.34761215
AGGTCGG406.160641E-428.1247945
CTCGTAT1150.027.39110639
AATCTCG1200.026.24980736
CAGGTCG450.001225468924.9998174
CGAGACC1101.5097612E-1024.54527523
ACTAGCC651.5212125E-524.230598
TCTCGTA1353.6379788E-1223.33316238
TCGTATG1353.6379788E-1223.33316240
ATGCCGT1353.6379788E-1223.33316244
ACGGAAC702.6985152E-522.49983640
GCAATCT1104.0363375E-922.49983634
Produced by FastQC (version 0.11.2)