FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 30h_92.34100000005b13.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 30h_92.34100000005b13.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences125978
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTAGGAGATTGTAAAGGGAGGTTTTGTGAAGTTCTAAAAGGTTCTAGTT1450.11509946181079236No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTTAG207.009971E-444.9992072
AGCCAGA207.009971E-444.99920715
TGCCGTC207.009971E-444.99920745
TAGTATG403.426121E-739.3743065
ACGAGAC301.1342783E-437.4993422
TTGTAAC250.002100189435.99936723
TATGCCG250.002100189435.99936743
ATGCCGT250.002100189435.99936744
GTTAGTA458.6196087E-734.9993863
GTATTGG352.7934628E-432.1678281
GCCCACG352.8065243E-432.1422918
CACGAGA352.8065243E-432.1422921
CCCACGA352.8065243E-432.1422919
AATCTCG352.8065243E-432.1422936
TCGTATG352.8065243E-432.1422940
CGAGCCC352.8065243E-432.1422915
CCACGAG352.8065243E-432.1422920
ATTTAGG300.005130519629.9994723
TTAGTAT554.126934E-628.6358574
CTCGTAT406.1360945E-428.12450439
Produced by FastQC (version 0.11.2)