FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 30h_91.34100000005b06.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 30h_91.34100000005b06.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences85284
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCAGCAGCAATCTCGTATGCCGTC1120.13132592279911823No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCACGA206.9965556E-445.00000419
AATCTCG206.9965556E-445.00000436
GCCCACG250.002096207336.018
TCGTATG250.002096207336.040
CGAGCCC250.002096207336.015
CAGACTG250.002096207336.05
ACGGGGT352.7986703E-432.1428616
CAGAAGT300.0051208830.00000222
CTCGTAT300.0051208830.00000239
TCCGAGC300.0051208830.00000213
CCGAGCC300.0051208830.00000214
CCTATTC300.0051208830.0000023
GACTGTG300.0051208830.0000027
TACGGGG755.5117198E-827.015
AGCAATC450.001217260725.00000233
CGAGACC450.001217260725.00000223
GCAATCT450.001217260725.00000234
GTATCAA1350.024.9999981
GGTATCA602.4416132E-422.5000021
CTCCGAG654.19405E-420.7692345
Produced by FastQC (version 0.11.2)