FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 30h_90.34100000005af8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 30h_90.34100000005af8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46042
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1290.2801789670301029No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT640.1390035185265627No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA550.11945614873376484No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT530.11511228877980975No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA651.3425961E-831.1538451
CACATCT751.6393842E-624.045
TATCAAC907.972361E-620.02
AACGCAG951.271584E-518.9473696
ACACATC1104.4839588E-516.36363644
ATCAACG1104.4839588E-516.3636363
CAACGCA1156.5590815E-515.6521735
TCAACGC1156.5590815E-515.6521734
ACGCAGA1251.3354488E-414.4000017
CGCAGAG1301.8636626E-413.8461538
TACACAT1301.8636626E-413.84615343
ATACACA1403.490713E-412.85714342
TATACAC1454.6937386E-412.41379341
GCAGAGT1558.224074E-411.6129049
CAGAGTA1558.224074E-411.61290410
AGAGTAC1400.00512701811.25000110
TTATACA1600.00107307911.2540
CTTATAC1650.001387903210.90909139
TCTTATA1850.00359230519.72973138
CTCTTAT1950.00554704679.23076937
Produced by FastQC (version 0.11.2)