FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 30h_9.341000000055e0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 30h_9.341000000055e0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences326643
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTGGAGAAAGAAATTCGTACATCTAGGAGCTATAGAACCTGTCTCTTA3420.10470146306518124No Hit
CTTATACACATCTCCGAGCCCACGAGACATAGACAAATCTCGTATGCCGTC3330.10194616140557122No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCCTA250.00210508135.999395
CGCCCTT250.00210508135.9993924
TGCCGTC702.0008883E-1135.3565445
ACGTGGA300.005142354429.9994936
AATCTCG901.2732926E-1129.99948736
ATATGTA1001.8189894E-1229.271911
GTAGACG702.8734576E-828.9280819
TTCGTTG406.1572326E-428.12452136
TGTGCGA406.1572326E-428.12452110
TATGCCG903.929017E-1027.4995343
TAGGTTG507.14381E-526.9995425
TACGGAT608.1691815E-626.24955638
ATGCCGT957.366907E-1026.0521944
CGTAGTC801.0491385E-725.31206924
CGTTGTC450.001224796824.99957338
GGTTATT450.001224796824.9995732
CACGAGA1001.3369572E-924.7495821
TCTAGTG551.3654337E-424.5450363
CGTATGC1052.3519533E-923.57102841
CTCGTAT1052.3519533E-923.57102839
Produced by FastQC (version 0.11.2)