FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 30h_87.34100000005ac1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 30h_87.34100000005ac1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences139875
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTCTTAGTATCTCGTATGCCGTC32302.3092046470062555No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG4450.043.48245243
ATGCCGT4450.043.48245244
TGCCGTC4450.043.48245245
TCGTATG4600.042.55367740
CTCGTAT4650.042.09610439
CGTATGC4600.042.06455241
GACGTCT4400.041.93115226
CGTCTTA4400.041.93115228
TAGTATC4400.041.93115233
CTTAGTA4350.041.89588531
AGACGTC4450.041.46001425
ACGTCTT4450.041.46001427
GAGACGT4550.041.043324
CGAGACG4700.040.69083823
AGTATCT4550.040.54880534
TTAGTAT4550.040.54880532
TCTCGTA4850.040.36018438
ACGAGAC4900.039.94834522
CCACGAG4900.039.94834520
GTATGCC4850.039.89627542