FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 30h_86.34100000005ab4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 30h_86.34100000005ab4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3696
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTCTTAGTATCTCGTATGCCGTC84722.916666666666664No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTCTTAGTATCTCGTATGCCG852.2997835497835495No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTCTTAGTATCTCGTATGCCGT411.1093073593073595No Hit
ATCCCTGACAAACTCATACATCAAATTGCACACCAGTTGGATAACATTTGT300.8116883116883116No Hit
CTTGTAGGCGTACGGTTTCAGGTTCTATTTCACCCTTCTATTCGAAGTGCT280.7575757575757576No Hit
CTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTC230.6222943722943723No Hit
GCTATCCTTTTTTCAATGGCATTTGTATTTACAGCTTGTTCAGGCAACAGT150.4058441558441558No Hit
ATGCGGCGGATCATCTCTTCGACGATGTCCATGATTTCATTTTCTTCCAGG140.3787878787878788No Hit
TATCAGTACTACAAGCACTCGTACTACAGTATTCACAAGTACTACCGCATT130.35173160173160173No Hit
GTACTTGCTACAGAAGATGATCCTGCGAATTCCTTACAAAAAAAAGCAACA130.35173160173160173No Hit
TTATACACATCTCCGAGCCCACGAGACGTCTTAGTATCTCGTATGCCGTCT120.3246753246753247No Hit
TGGAAGGATGGTTTAGGTGGAGTGAAAGAATGTGAAGAATGGAATGGAAGC110.2976190476190476No Hit
ACTAGAGAGTATGGACCAGTGCAGCATAGAATGACCTGTCAGTCCTTAAAA110.2976190476190476No Hit
GAACACAGGATTTCCTTACATCTTCTTCACGGGAAATGCGAACGCAAATAA110.2976190476190476No Hit
CCAACAGCTTGCTAACCTAACCTTCTCCGTCCCCACATCGCATTTGAATCA110.2976190476190476No Hit
ACATTACGCCAATTGCGATTGAGCAAGCGAAAACACAAAAGCCAACAGAAA100.27056277056277056No Hit
GGCTAGGGGCTGACAACAGTTACTGAACCCTATCAAACTCCGAATGCCATA100.27056277056277056No Hit
GAAACATCTAAGTACCCTGAGGAAAAGAAATCGAAGAGATTCCGTAAGTAG90.2435064935064935No Hit
TCATTGAAAGGAATCAGATGGAATCATTGAATCAAATCGAATGGAATCATC90.2435064935064935No Hit
GGATGGATCATCCTTTTTCGGTGAGTAACTTCAAGATCACGGATGAATCAC90.2435064935064935No Hit
GAAATCGCAGACACAAAACTGCATCGCATTGAACTGGATGCAAACAAAAGT90.2435064935064935No Hit
CGTTGAAATACCACCCTTTCTGTATTCGGTGTCTAACTCCTGTCTCTTATA90.2435064935064935No Hit
GTATAAGAGACAGGCATCATTCTACACCAGCATTTTCAGCTCATATAGGCA90.2435064935064935No Hit
TTCCTGACATTTTCACAAAAAGCATCTCCTTTAACTCCCTGGTTTCCCTGT90.2435064935064935No Hit
GAATAGAAGGGTGAAATAGAACCTGAAACCGTACGCCTACAAGCTGTCTCT80.21645021645021645No Hit
CTTCTTCACTTTGAACTCTAGCATAAGGGTCCCAACTATGAGCTCCTGTCT80.21645021645021645No Hit
ACCTGACAGTACTCAGAGTGCTTGCACGAAGAGATATGGACAAAGTAACGG80.21645021645021645No Hit
CTGAAAGACCGATAGTGAACAAGTACTGTGAAGGAAAGGTGAAAAGCACTT80.21645021645021645No Hit
ACTTACGGAATCTCTTCGATTTCTTTTCCTCAGGGTACTTAGATGTTTCAG80.21645021645021645No Hit
GAGCTGGATACTGTCGCGGAACTGGCCAATTAACTCTTTGACCACATGGTC80.21645021645021645No Hit
CCTGCGGTGTTCCGAACAGTCTATTAGCTATGTCACTTACACTATGCTGAA80.21645021645021645No Hit
CCCCTAATCAGACCTCCTCTCACTAGAGAATTGCATTTGTTGGAAAGACTC80.21645021645021645No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTC80.21645021645021645No Hit
AACTACCAGTTCATACAAAAGGAATTTCGCACTATTGAACGTCTCTTCAAC70.1893939393939394No Hit
TCTTTTATTTGGAACTTCATGGGTGAACATCATTCTCTAACCTCAAGATCA70.1893939393939394No Hit
GTCCATGGATAACGTTTTTGTGATCGCGCTAATATTTAGCTTTCTAGCGAT70.1893939393939394No Hit
GCTTAGTACCACTATTGAAATTTCGAATACGGGACTATCACCCACTATGGT70.1893939393939394No Hit
GACAAGAAACATCAAGACAAATCTATGGTCCATTCATCTCCTTTGAGAGCC70.1893939393939394No Hit
GTTAAAGCGGATCAAAGACAATCTGTCTTGTTCTTGTTCCTGTCTCTTATA60.16233766233766234No Hit
GTCTTTATTACTTACTCAACGGTCACACTCTTGGCCAGGTTACGTGGTTTG60.16233766233766234No Hit
ACTCAGATCAACTCCTACACCCTTTAACCAACTATTCCTTCAGTTGGCAGG60.16233766233766234No Hit
ATCGTGATCACACCATTTTTGCCTACTTTTTTGATCGCTTCTGCAATCCTG60.16233766233766234No Hit
TGCAAATCCCTAGAACTGACGCATAGGTAGAATTCACCTTAATCGTGACTT60.16233766233766234No Hit
GTTACCTGAGTATAGACAACACGTCTTTGTCATCTCAACATCTCCTGTATC60.16233766233766234No Hit
ATGTAGATGTCATGTCTTCAGAATGCAGATCGGTGTAAAGATCTGGGCCCT60.16233766233766234No Hit
GTATCAACGCAGAGTACGGGGTGGTATCACTGTCTCTTATACACATCTCCG60.16233766233766234No Hit
GACAACCACTCATACAGAAAGGAATTCCGCAATTCATACATCTTGCAGCTT60.16233766233766234No Hit
GAGACAGAGACAAGACCATAAAGTTATGGAATACTCTGGGTGTCTGCAAGT60.16233766233766234No Hit
ATCCCAAACTACTCGACTCTTGGAACGTGTATCACAAAGCACGGTACTTCC60.16233766233766234No Hit
CACTTTTTCTTCAGCAAATCATCATCCCTGTCTCTTATACACATCTCCGAG60.16233766233766234No Hit
GTTCGATGGAGGCCGTAATGGATAAAAACATGCAGTATTTGGTTGATTTCG60.16233766233766234No Hit
GCATGATGATATCCTTGCAAAGAAGAATAAAGTCAAGGCAACAAGAAGGAG60.16233766233766234No Hit
GGCATACGCACTTCAAAAAACTCATCTAAATTGGAGGCCACGATGCATAAA60.16233766233766234No Hit
CGTCAAAGCCTGATTGCAAGTTATATTCTGATGGGCATTTTTTTGCTTTCG60.16233766233766234No Hit
TAATCAGACTCTTGTAATGGTGGTGTTGATGGGGGTGGTGATGGTTGGTGG60.16233766233766234No Hit
TCAACAGCATATGGTCTTAGCTTGTGGCAATGATAAGCCTGTCTCTTATAC60.16233766233766234No Hit
CCTTAGGCCTGAAAACGCTCGAAATATCCACTTCCAGATACGACAGAAACA60.16233766233766234No Hit
GGTATCAACGCAGAGTACGGGAATGGAATCATTGAATGGACTCGAAAGCTG60.16233766233766234No Hit
TCCAAAAACAAACAAACAAAAAAAGTTGCCTTGTCATTCCTTTCCAACACA60.16233766233766234No Hit
TCACTGAGTGGTCAGTATGCGGATACTGATACATATGAGCACGAAACTCAG50.13528138528138528No Hit
ACTCAGATCAACTCCTACACCCTTTAACCAACTATTCCGTCAGTTGGCAGG50.13528138528138528No Hit
ACTAAGGGAATCACTTTTGTTTTCTTCTCCTATGGGTACTTAGATGTTTCA50.13528138528138528No Hit
GGAGAGCAATAGTTTTCAGATGCAATCAGCTCAATATGCGCTGTCTCTTAT50.13528138528138528No Hit
CAGTAAGTCAGACAACACTTGCCACAGTAATGCCTGTCAAAGTGCTGTCTC50.13528138528138528No Hit
GAATGTACTCACAACGATCTTACCTTTAATGGATTTAGCGCAACATTTTAG50.13528138528138528No Hit
CTGAAAGACCGATAGTGAACAAGTACTGTGAAGGAAAGGTGAAAAGCACCT50.13528138528138528No Hit
GTTCACTATCGGTCTTTCAGGAGTATTTAGCCTTGGAGGACTGTCTCTTAT50.13528138528138528No Hit
ACCTGACAGTACTCAGAGTGCTTGCACGAAGAGATATGGAAAAAGTAACGG50.13528138528138528No Hit
CTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGTC50.13528138528138528No Hit
CCTTATGAAGAAGAACGATGTCAACAAGTCTCTGCTACAATACCTGTCTCT50.13528138528138528No Hit
ATCACGGGGTGCAGGATATCAACCACACTGGCAATATCTTTATCAGACAGC50.13528138528138528No Hit
ACTTGGGACGTTATAACCGCGATCTATGATGCCTGTCTCTTATACACATCT50.13528138528138528No Hit
GTACATGATGTTTTCGATTTAGAAAATTTGGCGCGGAGAAAAGTTGACTAC50.13528138528138528No Hit
CCTTTAATTCTGCTAGCTTTGGGGTGGATTTGTTCATGCTTCTCTAATTCT50.13528138528138528No Hit
GTAGTGAAATTGCCCATTTAAGTTTGTGAAGGTGCAGGCCTGTGAAAACAG50.13528138528138528No Hit
ACGTCGAACAACGGCTCTTTGTCTTCCTGATTGTCTTTGTTGTCTGTCTCT50.13528138528138528No Hit
GAGTCGAGTTGTTTGGGAATGCAGCTCTAAGTGGGTGGTAAATTCCTGTCT50.13528138528138528No Hit
CACCTAATGTGTTTATGAATCAGGCTCCGTTTGCATTTGATTTGTCAATGT50.13528138528138528No Hit
TACTCTGGTAAGACTATACAGAAGACTGATTGGTCTTTAGCCCCTAGCATT40.10822510822510822No Hit
CCTATAAACAACGCAATAGTAAAAAACGGCAACAAATCTAACCGCCGCAAA40.10822510822510822No Hit
AACGTAACTAGATTTAGATGCTGTAAGAGATACGCAAATACTTGAGGGTCG40.10822510822510822No Hit
ACTCTGTTAGTTGAGGACACACATCACAAATAAGTTTCTGAGAATGCTTCT40.10822510822510822No Hit
CATTAGGTGCGGCTAAATTAAGCTGCTCTTTCATCCATTTCATCAGGTTGA40.10822510822510822No Hit
GCTTTAGTTAGCCTACTGCGTCCCTCCATCACTTCATACTCTAGTACAGGA40.10822510822510822No Hit
CTATCGGTCTTTCAGGAGTATTTAGCCTTGGAGGATGGTCTGTCTCTTATA40.10822510822510822No Hit
AACTTGTTGTAGGCACACGGTTTCAGGTTCTATTTCACCTGTCTCTTATAC40.10822510822510822No Hit
ACTACTTCCTTCCTACAACTTGGAAGTGAAGGAGGTTCTGTGCCTGTCTCT40.10822510822510822No Hit
CCACTGTAGTCAAATTACAACCAAAACCCCCCGACTAATGCTGTCTCTTAT40.10822510822510822No Hit
GTCTGGATTCACTATCGCGAAGACGTGATCAATGATGATCAGTTCCTGTCT40.10822510822510822No Hit
ATCAACGCAGAGTACGGGGGAAGAGAAAGAAAAATCGATTTCCTAAGTAGC40.10822510822510822No Hit
GTATGAATCTATGCCCGCTGTACCCTCATGCTGTCTCTTATACACATCTCC40.10822510822510822No Hit
TTATTAAACCCCTGAAATTTCAGACGGCATGAGTTTTGTCCGTTTTTCACG40.10822510822510822No Hit
TCTTTGTGATGTTTGTATTCAGGACACAGAGTTGAACATTCCCTGTCTCTT40.10822510822510822No Hit
GGTTTTAGGGTCTGGCATAAGATGGGTTTTTCAAGGCAGGGACTGTCTCTT40.10822510822510822No Hit
GGATTAGAAGCCGTTTCTAGGGGATATGATAGTGCAGTTTTTGTAGCTGTC40.10822510822510822No Hit
ATCAAGTCCAGACAAACTCCGAATGCACGTTAAGTACTGTCTCTTATACAC40.10822510822510822No Hit
CATGATTAGATAGTGCCGATTTTTTGGGTTGCAAAAAATATAGGCACGAGG40.10822510822510822No Hit
GGTGGGCCGTAGACTTAAGTGAACTTGAAAACGAGTTTTTGACTCCGTCGG40.10822510822510822No Hit
GGGTTAATACCTCTGTTTGATGACGGTACCTGAAGAATAAGCACCGGCTAA40.10822510822510822No Hit
GGCGTGGATAATAATCGGGCATAAGCTATTTACCGAAGCTGTGGGATGTGT40.10822510822510822No Hit
CCCTTGGAAGATGGAACTCTGGCAGAACTTTTTGTGCGATGTGTATTTTGT40.10822510822510822No Hit
CAGGTACATGGGTGACAATGACGATCTGAAGTTGAATCCACGGTTCCTGTC40.10822510822510822No Hit
ATTCAAAAGGAGTCCACCCTTCATCTGGTGTTGAGCTGTCTCTTATACACA40.10822510822510822No Hit
GAACTGGGTGACTAGAGTGTGTCAGAGGGAGGTAGAATTCCACGTGTAGCA40.10822510822510822No Hit
CACTATTTCATGGGCCAGGCATACTGCAATAGCTGTCTCTTATACACATCT40.10822510822510822No Hit
TGTCAGGACTATGTTTATTGTCCGATTTAGGCTTCAGGATGACCGGCAGCT40.10822510822510822No Hit
TTTTTGCATTGCTTCAAAATGGGTGATGCGGTCACTCCTGTCTCTTATACA40.10822510822510822No Hit
GAATCAGATAATGCCCTACGGCTTTCGGTACACTGATCCTGTCTCTTATAC40.10822510822510822No Hit
CATTCTCGTCCGGAAGCTGAGGAAACTTTGAAAACCATTGAGGAAAATGGC40.10822510822510822No Hit
TGTAGGACTAGAACGTGAGAAGAAATCAAGATAGCAGAAGTGGTCTGGAAA40.10822510822510822No Hit
AAGAAGATGTGTTAGGATCGTTTCTAGGAGAATGCTTATGATCTGTCTCTT40.10822510822510822No Hit
GGGTTAATATTCCCATACTTGCTCACACTAAAAAGGGGACGGAGTGACGTA40.10822510822510822No Hit
GGATGTTTGCTTGGAAGCAGCCATTCATTTAAAGAGTGCGTAACCTGTCTC40.10822510822510822No Hit
CACACACACACACACAAAACAGAAAGAAAACAGATGAGCAACGAGTTTGAC40.10822510822510822No Hit
GCTGAGTATGGTGGCTCATGTCTATAATCCTAGTACTTTGTGAGACCAAGG40.10822510822510822No Hit
GTATCTTTATCACGCTTTAAATATCAGGCAGAGGATAGTGTTAGGTAGCAG40.10822510822510822No Hit
CCAGGCTTTCCGAAAATGGTTGCTATGACCACAAACCTAAAGACCTGTCTC40.10822510822510822No Hit
ATACCAGATATGGACAAAGTCAGGCCGTCTTGATCCTTTTGGATAATCGAT40.10822510822510822No Hit
GAAATAATAGCTAAGTTGACCAAACGAAGTCAGAGTGCGAAGACCTGTCTC40.10822510822510822No Hit
ATTTAGACCAAATGAGATCACAAAGGAATGCAGCTGTCTCTTATACACATC40.10822510822510822No Hit
AACCAGTACCGTGAGGGAAAGGTGAAAAGAACCCCGGAAGGGGAGTGAAAG40.10822510822510822No Hit
CTTTCAATGATTATTCCATTCGAGTCCATTCGGTCTGTCTCTTATACACAT40.10822510822510822No Hit
GGGTAGGGGTGAAAGGCTAATCAAACTTGGAGATAGCTCGTACCTGTCTCT40.10822510822510822No Hit
CACGGCGATCAGCTTCAAATTCTTTTCTTTGATGATATGCGACAGAAGCTG40.10822510822510822No Hit
AACAACAGCAGTTACTCAAACATCAGCAGCAACTTGAGCCACAAATTTCAA40.10822510822510822No Hit
AGCATGGCTGAGGAAACCTTAGTCTTTCGGTGAGGGGGTTTCTCGCCCCCT40.10822510822510822No Hit
TATCAACGCAGAGTACGGGCAGTGGTATCTGTCTCTTATACACATCTCCGA40.10822510822510822No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC850.039.70588345
ATGCCGT900.037.544
TATCTCG850.037.05882336
GTATCTC750.036.00000435
AGTATCT750.036.00000434
TAGTATC750.036.00000433
TTAGTAT750.036.00000432
AGCCCAC1000.036.017
GCCCACG1000.036.018
CCACGAG1000.036.020
GAGCCCA1000.036.016
CACGAGA1000.036.021
CCCACGA1000.036.019
ACGAGAC1000.036.022
CGAGCCC1000.036.015
CCGAGCC1000.036.014
TCTCCGA1000.036.011
TCCGAGC1000.036.013
CTCCGAG1000.036.012
GTATGCC950.035.52631442
Produced by FastQC (version 0.11.2)