FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 30h_85.34100000005aa8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 30h_85.34100000005aa8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences273278
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTCTTAGTATCTCGTATGCCGTC3120.11416945381626037No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGCAA253.883723E-544.99853524
AGTATCG250.002102750836.0054178
GTATCGA250.002102750836.0054179
CGTACAG401.5546306E-533.75507710
TACTACT300.005141292229.9990234
TACGACG406.148608E-428.1292325
ACGGGGT1200.026.24914616
CGACGGT450.001223088825.0037637
ACGACGG450.001223088825.0037636
CTAAGGC450.001224394924.9991873
GTCTTCG551.3647799E-424.54465522
CGAGACG651.5186175E-524.2299823
TGCCGTC651.5186175E-524.2299845
CGGGGTG952.1011147E-823.68344117
AGAAGTC702.690134E-522.5033865
AGTGCAA500.002258251722.50338610
TCGTACA602.4583313E-422.5033869
GCGTCCC500.002258251722.50338611
TATGCCG702.6939419E-522.4992743
CCACGAG702.6939419E-522.4992720
Produced by FastQC (version 0.11.2)