FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 30h_84.34100000005a9b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 30h_84.34100000005a9b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences232414
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG458.662864E-734.9986643
CGTCTTA352.8105814E-432.1485528
GTATAGC300.005134465630.0053061
CGAGACG453.479905E-529.99885423
CCCACGA602.405759E-729.99885219
GCCCACG554.147407E-628.6352718
CGTATGC554.147407E-628.6352741
ATGCCGT554.147407E-628.6352744
TGCCGTC554.147407E-628.6352745
CGGGATG406.152608E-428.12392417
TAGTATC406.152608E-428.12392433
TATCTCG608.157278E-626.24899536
GACGTCT450.001222349225.00442526
AGACGTC450.001222349225.00442525
TTACTCT450.001223884224.9990444
GTATCTC450.001223884224.99904435
CTCGTAT702.69182E-522.4991439
TCTCGTA702.69182E-522.4991438
CCACGAG802.95883E-622.4991420
CACGAGA754.583724E-520.99919721
Produced by FastQC (version 0.11.2)