FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 30h_83.34100000005a8e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 30h_83.34100000005a8e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences200932
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG250.002103209735.99920343
TGCCGTC300.005137827729.99933445
ACGAGAC450.001223191624.99944722
TAGACAG500.002258450522.4995025
TATTGGA500.002258450522.4995022
TTTACAC550.003925916320.4540943
AGTGCCA550.003925916320.45409410
TAGATCA550.003925916320.4540945
GTATCAA2000.020.269731
GGTATCA1351.9117579E-920.0194871
GTATTGG706.910581E-419.3045061
GAGTACT706.9570896E-419.28528611
TACGAGG706.9570896E-419.28528620
GTGTAGC600.006455824718.7682691
CGTATGC600.00649307518.74958441
GCTTACT600.00649307518.7495842
CCGCAAG600.00649307518.74958423
AAGTCAT600.00649307518.7495847
AGCATTA901.8563407E-417.4996135
ATCTCCG1853.0013325E-1017.0266545
Produced by FastQC (version 0.11.2)