FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 30h_82.34100000005a71.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 30h_82.34100000005a71.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences142327
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTCTATGTGGCAAAATAGTGAGAAGATTTTTAGGTAGAGGTGAAAAGCCT2960.20797178328778096No Hit
TCCCAATGGTGTAGAAGCTATTAATGGTTCGTTTGTTCAACGATTAAAGTC2900.2037561390319476No Hit
GTGTAGAAGCTATTAATGGTTCGTTTGTTCAACGATTAAAGTCCTACGTGA2270.1594918743456969No Hit
TCTAGGAGCTATAGAACTAGTACCGCAAGGGAAAGATGAAAGACCTGTCTC1920.1349006161866687No Hit
ATCCTATTCCCATCCTCAAAACGCCTAATCAACAACCGTCTCCATTCTTTC1820.1278745424269464No Hit
CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAAC1810.12717193505097416No Hit
CTTATACACATCTCCGAGCCCACGAGACGTCTTAGTATCTCGTATGCCGTC1750.12295629079514077No Hit
GTGTTGGAAAGAATGGAGACGGTTGTTGATTAGGCGTTTTGAGGATGGGAA1620.11382239490750175No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCCAG253.8747916E-544.9975418
CCCCAGC253.8747916E-544.9975419
ACCCCCA253.8747916E-544.9975417
TGCCGTC207.014335E-444.9975445
GTTGCAA207.014335E-444.997548
CCACACC207.014335E-444.9975436
CCCAGCC301.1352678E-437.4979520
GCCCACG250.00210148735.9980318
CTCGTAT250.00210148735.9980339
GACGTCT250.00210148735.9980326
CGTCTTA250.00210148735.9980328
TATGCCG250.00210148735.9980343
GCCATGT250.00210148735.9980322
GCTCTCT250.00210148735.9980344
ACGTCTT250.00210148735.9980327
ACCACAC250.00210148735.9980335
GGTTGCA250.00210148735.998037
CTTAGTA250.00210148735.9980331
TGAGATT458.6088585E-735.01038710
TTGAGAT458.6325963E-734.998099
Produced by FastQC (version 0.11.2)