FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 30h_81.34100000005a64.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 30h_81.34100000005a64.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46211
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT780.16879098050247776No Hit
CTTATACACATCTCCGAGCCCACGAGACGTCTTAGTATCTCGTATGCCGTC550.119019281123542No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT490.10603535954642833No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAATTT250.002086288836.013
TCTGGGA250.002086288836.034
AACGCAG2250.025.06
CGGGGTG551.3406533E-424.54545417
ACGCAGA2400.023.4375027
GGGGTGG602.4181523E-422.50000218
TACGGGT500.002232121822.515
CGCAGAG2550.022.0588258
AGAGTAC2600.021.63461511
GTATCAA2650.021.2264161
GCAGAGT2650.021.2264169
CAGAGTA2650.021.22641610
TATCAAC3050.018.4426232
GAGTACG2100.018.21428512
AGTACGG2100.018.21428513
ACGGGGT750.001088456618.016
GGTGGTA750.001088456618.020
CAACGCA3150.017.8571435
ATCAACG3150.017.8571433
TCAACGC3150.017.8571434
Produced by FastQC (version 0.11.2)