FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 30h_8.341000000055c6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 30h_8.341000000055c6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences188
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGATATAAATCTCGTATGCCGTC5227.659574468085108No Hit
CTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTC1910.106382978723403No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGTC84.25531914893617No Hit
CTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGTC42.127659574468085No Hit
CTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTC42.127659574468085No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTC31.5957446808510638No Hit
CTTATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCGTC31.5957446808510638RNA PCR Primer, Index 27 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACAGATATAAATCTCGTATGCCGGC21.0638297872340425No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTC21.0638297872340425No Hit
GGTTCTCTCCGAAATAGCTTTAGGGCTAGCCTCGCGAGAGAGGATAATGGA10.5319148936170213No Hit
GGTATGAGTAGCGAAAGATAGGTGAGAATCCTATCCGCCGAAAGACTAAGG10.5319148936170213No Hit
GAATGGAATACAATGGAATGGATTCAACTTGAATGGAATGGAAAGAATGGA10.5319148936170213No Hit
CTTATACACATCTCCGAACCCACGAGACTACTTGCAATCTCGTATGCCGTC10.5319148936170213Illumina PCR Primer Index 12 (95% over 21bp)
TTGTAGGACCTGGAATATGGCGAGAAAACTGAAAATCACAGAATATGAGAA10.5319148936170213No Hit
GGTCTACGTCAAGTAACTGCTCGCCCTATTCAGACTCGCTTTCGCTGCGGC10.5319148936170213No Hit
GTACTAGTTCTATAGCTCCTAGATGTACGAATTTCTTTCTCCAATACCTGT10.5319148936170213No Hit
CATGTAAATTTGGGTAAATGAGTTTGTCCAATTGGCTTGTTGAGGGACAAA10.5319148936170213No Hit
CGGGTGGGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGATATAA10.5319148936170213No Hit
CAAAGATACAGGAACCCAAGAGAGGAGGAAAACTGGCAGAGCTGTCTCTTA10.5319148936170213No Hit
CTGATACACCACAGCTACATTAACCAACAGCCATACCTGTCTCTTATACAC10.5319148936170213No Hit
CACCAAGGTCTGCCACTAAAGGCCTATTTCTCTGTCCTCGTTCCAATTGCC10.5319148936170213No Hit
GTTCGCTATCGGTCTCTCGCCAATATTTAGCTTTAGATCTGTTTCTTATAC10.5319148936170213No Hit
CTGCCTAATAGTCCAAATCATTACAGGTCTTTTTCTTAGCCATACACTACA10.5319148936170213No Hit
AAACGATAAAGCCGGAGGATAGTTCAACAACATCTGTCTCTTATACACATC10.5319148936170213No Hit
CTTATACCATCTCCGAGCCCACGAGACAGATATAAATCTCGTATGCCGTCT10.5319148936170213RNA PCR Primer, Index 39 (95% over 21bp)
TTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTCT10.5319148936170213RNA PCR Primer, Index 44 (95% over 22bp)
AGTCCAAACAGTGCTCTACCTCCATTATCCTCTCTCGCGAGGCTAGCCCTA10.5319148936170213No Hit
GGTCTGGGACATCTCATCGCACGGGATTCTCACCCTCTGTGACGTTCCTGT10.5319148936170213No Hit
ACAGCTTATACACATCTCCGAGCCCACGAGACAGATATAAATCTCGTATGC10.5319148936170213No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTAAAA10.5319148936170213No Hit
GGCTAGAAGCTTTTCTCGGCAGTGTGACATCATGACCTTCGCTACTTTATT10.5319148936170213No Hit
TTATACACATCTCCGAGCCCACGTGACAGATATAAATCTCGTATGCCGTCT10.5319148936170213RNA PCR Primer, Index 39 (95% over 21bp)
TATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGTCTT10.5319148936170213Illumina PCR Primer Index 4 (95% over 21bp)
TTCCAAGAGAGAGCAGTGGAAGAAGCTGAGGATGGAGAGCTGGGATCGGGA10.5319148936170213No Hit
CTGTAACTACATCTGGAGTTGGTTCTACTTTCTTGTAGCTGTCTCTTATAC10.5319148936170213No Hit
GTCTTAACCTCGCTACTTAACGTAACTCGCCGGTTCATTCTACAAAAGGCA10.5319148936170213No Hit
ATATATGCCCTGCTTATAACAATGTCTGCAGTGACTATATAGCCTAGAAAA10.5319148936170213No Hit
ATTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTC10.5319148936170213No Hit
CCATGGAGAGGAGGAGGTGGAGACCTTTGCCTTTCAGGCAGAAATTGCCCA10.5319148936170213No Hit
TTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGTCT10.5319148936170213Illumina Paired End PCR Primer 2 (95% over 21bp)
CTTATACACATCTCCGAGCCACGAGACAGATATAAATCTCGTATGCCGTCT10.5319148936170213No Hit
GCTTCAGACTTCTCGGAAAGCGGATTTGCCTACTTTCCATCCTTTGCGCTG10.5319148936170213No Hit
GGCATGATGATGTGAATGGTATGATTTTTGTGATGTTTCCTATTTTTCGGA10.5319148936170213No Hit
TTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGTCT10.5319148936170213No Hit
TGGTAGGAGGGCGTTCTAAGTGCGGTGAAGTCTAATCGCTGTCTCTTATAA10.5319148936170213No Hit
CTACAGACGAAAAAGCTGAAGACGCTAAAGAAGATATTGATCAAAAAGCAG10.5319148936170213No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGATCTTGGGAGCGGG10.5319148936170213No Hit
GCCTACTACGACAACTAAAATTTCACTTCACATCAAAACATCACTTCGGAT10.5319148936170213No Hit
ATTCAGGTTAAGATAAGTAGGTTGGCTACTAGGATTCAGTACAAAATTTGT10.5319148936170213No Hit
CAATCAAACACCGTGATAGCTGGTTCTCCCCGAAATGCATTTAGGCTGTCT10.5319148936170213No Hit
CTTATACACCATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGT10.5319148936170213No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAGAG10.5319148936170213No Hit
CTGCTGGTGACCCATCTTGCCTTCAGACGCCCAACGAGGAGAAAGGCCCGT10.5319148936170213No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTAGTATGCCGTC10.5319148936170213No Hit
AAACAGGCTGTCCTAAAACCCTTTCTGAGATGAGTCTGATTCCTGTCTCTT10.5319148936170213No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTGGCCAAACATGGGTATAA10.5319148936170213No Hit
AGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10.5319148936170213No Hit
CTCCACCTTAAATTTCTCGTGCCGCCCCTTCAGGCAGTCGATCTCGGACTG10.5319148936170213No Hit
CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTGA10.5319148936170213No Hit
ATTCCATTCCATTCCATTCCATTTCGTTCTTTTCCATTCTATTCCGTACCA10.5319148936170213No Hit
AAGTTCCCCAGAGCGACTAATTTCTTCAAACAGAGCTGTCCTCTCCTTGGG10.5319148936170213No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTGCAAACCTGTGTCA10.5319148936170213No Hit
ACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGTCTTCTG10.5319148936170213Illumina PCR Primer Index 4 (95% over 24bp)
GAATTGAACCCGGGTCCTTCGCAACAACAAGTGCTCTTAACCCCTGACCCA10.5319148936170213No Hit
CTACCGTCTCGTCTTCCTCTGCTGCCTCTGCTCGGTTCTTAGAATTCATCT10.5319148936170213No Hit
CCTTTAAATTGGACTTGGTACATGGGTAATGAAACTGCACCTGTCTCTTAT10.5319148936170213No Hit
TGCCTACAAGTAGTCAAAGCACATTAAAGTGTAATGGGCCCTTTTGTAGAA10.5319148936170213No Hit
CCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAACTGTCTCTTATACAC10.5319148936170213No Hit
CTTATACACATCTCCGAGCCCACGAGACCAAGGCGAATCTCGTATGCCGTC10.5319148936170213No Hit
GTACTACAGGCCTCTCAGGCTTACATCGTACATACAGCCAGGACCTGTCTC10.5319148936170213No Hit
GAAGGCCCCCAAGAGCTTGAGGGTCTGCGCAGGTTGGAAGCTGGGAAATGT10.5319148936170213No Hit
AAAGTACTCTGCGTTGATACCACTGCTTGGCCAAAAAGAAAAAAAAAAAAA10.5319148936170213No Hit
GGTCTGGCTCTTGTCTATGTTTCTTGGAACAGAACGTCACAGAGGGTGAGA10.5319148936170213No Hit
CTTATACACATCTCCGAGCCCACGAGACAGATGTAAATCTCGTATGCCGTC10.5319148936170213No Hit
GAAAATGAGAAACATCCACTTGACTTGAAAAATGACGAAATCACTGAAAAA10.5319148936170213No Hit
GTCCTACAGTGTGCATTTCTCATTTTTCACGATTTTCAGTGATTTCGTCAT10.5319148936170213No Hit
GCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAAAAAAAATTAT10.5319148936170213No Hit
GCATAATGACATCACGTCAAACTTTGTCTCCCCAACTTCGTATTTTTTGAT10.5319148936170213No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCC10.5319148936170213No Hit
CCCAAAAACCGAGTAATTGGAAGTGGTTGCAATCTGGATTCAGCGCGGTTC10.5319148936170213No Hit
CTATCCAACAGATCAGGAATTGGATGAAATGCCAGTTTCAAAGAGAACGTT10.5319148936170213No Hit
CTTATACACATCTCCGAGCCCACGAGCCTGCGACCTATCTCGTATGCCGTC10.5319148936170213No Hit
ATGTTTTCTAATTGCCTCATCTTCTTCAAGATCTTCAAGAAAATCTTGATA10.5319148936170213No Hit
CTTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCGTC10.5319148936170213RNA PCR Primer, Index 46 (95% over 21bp)
CCATATATCTATACGCGGGAGTCAGACTGCGAGTGATAAGATCCGTAGTCG10.5319148936170213No Hit
CTTCTGGAGTATGTGAGGATAGCTACAGTGTACTTACTGTCTCTTATACAC10.5319148936170213No Hit
CTTATACACATCTCCGAGCCCCACGAGACAGATATAAATCTCGTATGCCGT10.5319148936170213No Hit
TCATTATATTTCCTTCAATCCTATTCCCATCCTCAAAACGCCTAATCAACA10.5319148936170213No Hit
AAGCTGTTGTTATGGTCTTTCCAAAGAAACTAGTCACAGTGTCATCTTAAT10.5319148936170213No Hit
GTGTATGTACCTAGCTGTACTATATGTAGTTGGTCTGTCTCTTATACACAT10.5319148936170213No Hit
TTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTCT10.5319148936170213Illumina PCR Primer Index 11 (95% over 21bp)
ATTCAAAGAGTGCGTAATAGCTCACTAGTCAAGTGACTCTGCGCCGAAGAT10.5319148936170213No Hit
GTGGTGGACCTCATGGCCTACATGGCCTCCAAGGAGTAAGAAACCCTGGAC10.5319148936170213No Hit
CTTATACACATCTCGAGCCCACGAGACAGATATAAATCTCGTATGCCGTCT10.5319148936170213No Hit
AACTCAGAGATTCACCTGCCTCTGCCTCCCGAGTGATGGGATTAAAGGTGT10.5319148936170213No Hit
CTTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTC10.5319148936170213TruSeq Adapter, Index 12 (95% over 21bp)
GCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAGTACTCTCCGTTGAT10.5319148936170213No Hit
GTCCGATAGCTTGTCCCGGCCCTCAGCATCCCTCACTTCTGCTCCTGCAGG10.5319148936170213No Hit
AGGCTCTAACCCCAGAGCCCCCGGGGCTCATTTCAAGAAACGTCTTCTCCT10.5319148936170213No Hit
CTTTAGGGATGTATCTGGATATCAGAGGTACTACTGCTGTCTCTTATAAAC10.5319148936170213No Hit

[WARN]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.2)