FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 30h_79.34100000005a4b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 30h_79.34100000005a4b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1030
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTCTTAGTATCTCGTATGCCGTC747.184466019417475No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTC646.213592233009709No Hit
CTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTC222.1359223300970873No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGTC201.9417475728155338No Hit
CTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGTC171.650485436893204No Hit
CTTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTC151.4563106796116505TruSeq Adapter, Index 12 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTC151.4563106796116505No Hit
CTTATACACATCTCCGAGCCCACGAGACGATCGTGTATCTCGTATGCCGTC70.6796116504854369No Hit
CTTATACACATCTCCGAGCCCACGAGACAGTGTCTTATCTCGTATGCCGTC70.6796116504854369No Hit
CTTATACACATCTCCGAGCCCACGAGACTCAACTGTATCTCGTATGCCGTC70.6796116504854369No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAA40.3883495145631068No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40.3883495145631068No Hit
TTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTCT30.2912621359223301TruSeq Adapter, Index 12 (95% over 22bp)
TCTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGT20.1941747572815534No Hit
CTTATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCGTC20.1941747572815534RNA PCR Primer, Index 27 (95% over 21bp)
CTCTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCG20.1941747572815534No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC204.1667453E-445.045
AGCCCAC150.00939052144.99999617
GCCCACG150.00939052144.99999618
GTATGCC250.001254007636.042
ATCTCGT250.001254007636.037
ATCTCCG250.001254007636.010
TATGCCG250.001254007636.043
CATCTCC250.001254007636.09
TCTCGTA250.001254007636.038
CGTATGC250.001254007636.041
CGAGCCC250.001254007636.015
CCGAGCC250.001254007636.014
TCGTATG250.001254007636.040
TCTCCGA250.001254007636.011
TCCGAGC250.001254007636.013
CTCGTAT250.001254007636.039
CTCCGAG250.001254007636.012
CACATCT300.003077203429.9999987
ACATCTC300.003077203429.9999988
ACACATC300.003077203429.9999986
Produced by FastQC (version 0.11.2)