FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 30h_78.34100000005a3e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 30h_78.34100000005a3e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences353558
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGATTACAATCTCGTATGCCGTC3980.11256993194893058No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCCGAC356.236696E-638.570827
GCCGACC301.1384288E-437.4994138
CACGAGA551.12411726E-732.72675721
AGCACCG450.001224987324.9996075
GTATCAA7850.024.3764651
CTCGTAT851.8862193E-723.82315439
ATCCTAT903.2563003E-722.512381
CCCACGA802.9655039E-622.49964719
TCGTATG802.9655039E-622.49964740
ATGCCGT802.9655039E-622.49964744
CGCCTAA702.696488E-522.49964522
ACCGTTC500.0022617422.4996458
GGTATCA4250.021.7178271
GCCCACG855.016642E-621.17613818
TATGCCG855.016642E-621.17613843
CTAATCA754.5916233E-520.99966825
TAAAACC654.2306862E-420.7689044
CACCGTT654.2306862E-420.7689047
AATCTCG550.003931590420.45422436
CCTATTC1109.936775E-820.4542243
Produced by FastQC (version 0.11.2)